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Application of the Jerusalem artichoke (Helianthus tuberosus L.), as a plant origin medium additive, during the micropropogation of Ada keiliana
61-64.Views:314A procedure for in vitro propagation of Ada keiliana seedlings are suited for acclimatization, was worked out. M medium was supplemented, with Jerusalem artichoke, as plant origin complex additive. The apply of JAD (1,5g/flask) gave the best response, considering the shoot (29 mm), and the root development (24,9) mm) too. The plantlets with satisfying growth (25-30 mm, 4-5 roots) were transferred in small pine bark: Novobalt peat: coconut fibres: perlit (2:3:1:1) mix, among greenhouse circumstances.
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Susceptibility of some traditional pear cultivars of Hungarian and foreign origin to the pathogenic bacterium Erwinia amylovora
41-45.Views:320Fire blight, a disease caused by the bacterium Erwinia amylovora (Burrill) Winslow et al., has been causing serious damage in Hungarian pear plantations since 1996. A prospective control measure could be the use of resistant cultivars. For that purpose ten pear cultivars have been tested under laboratory and greenhouse conditions for resistance to Erwinia amylovora strains collected in Hungary. Six of these cultivars are Hungarian ones of unknown origin, while four are traditional old varieties. Resistant cultivars should serve as germplasm for future breeding. Inoculations were made with a mixture of different pear isolates of the bacteria collected from various growing regions of Hungary (Ea 21, 23), at a density of 5x108 cells/ml. Susceptibility/resistance has been assessed on the basis of intensity of blight symptoms observed on shoots, flower parts and fruits. Cultivars were assigned to three susceptibility groups (low, moderate and high). Complete resistance was not found among the cultivars tested. The highest level of resistance was found in cultivar 'Kieffer', while the other cultivars displayed either moderate or high susceptibility to infection.
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Effects of irrigation frequency and repellent plants on false codling moth (Thaumatotibia leucotreta) infestation, growth, yield, and quality of sweet pepper (Capsicum annuum L.)
34-40.Views:202Sweet pepper (Capsicum annum L.) is an important vegetable and spice crop grown worldwide for its pungency, which is derived from high concentrations of capsaicinoids. Despite the economic importance of sweet pepper as an export vegetable in Kenya, its production is currently constrained by the false codling moth (FCM) (Thaumatotibia leucotreta) and inadequate management strategies for the pest, among other factors. This study, therefore, evaluated repellent plants (Artemisia (Artemisia absinthium), Lavender (Lavandula angustifolia), and Spearmint (Mentha spicata) under different irrigation frequencies to control FCM. A split-plot factorial greenhouse experiment was conducted at Kenya Agricultural and Livestock Research Organization, Muguga (trial 1) and the Horticulture Research and Teaching Field, Egerton University, Kenya (trial 2). Irrigation frequency at three levels (irrigating once a week, irrigating twice a week and irrigating thrice a week) was the main-plot factor, while repellent plants at four levels: sweet pepper (Capsicum annum L.), artemisia (Artemisia absinthium), lavender (Lavandula angustifolia) and spearmint (Mentha spicata) constituted the sub-plot factor. Irrigating thrice or twice a week and using lavender as a repellent plant significantly reduced false codling moth infestation by 90%, increased yield by 28% and improved fruit quality in terms of fruit collar diameter and total soluble salts (TSS). The findings demonstrate that integrating lavender repellent plants with frequent irrigation can be adopted as an eco-friendly management strategy for FCM and enhancing sweet pepper yield and quality.
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Micropropagation of an orchid Dendrobium strongylanthum Rchb.f.
61-64.Views:556A simple and reliable procedure for in vitro propagation of an orchid Dendrobium strongylanthum Rchb.f. was studied. Protochorm was induced from seed explants on 1/2 MS medium supplemented with 0.2 mg/L NAA. A mass of protochorm could be multiplied on proliferated medium of 1/2 Ms containing 0.5 mg/L V-6-BA. And bud differentiation of green global body was cultured in the same media, 2-2.5 cm shoots were formed after 30 day of culture. Addition of mashed banana and 0.5 mg/L NAA to 1/2 MS medium promoted root formation and vigorous growth. The plantlets were acclimatized and transplanted to compound materials of humus:sawdust (1:1) in greenhouse, the survival rate was more than 98%.
