Effects of nitrogen fertilizer application rate and time to first harvest on leaf area index; leaf nitrate content and yield of irrigated leaf rape (Brassica napus L. var. Giant)

July 21, 2021

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Masaka, J., Chandiposha, M., Makaure, B. T., & Mazina, B. (2021). Effects of nitrogen fertilizer application rate and time to first harvest on leaf area index; leaf nitrate content and yield of irrigated leaf rape (Brassica napus L. var. Giant). International Journal of Horticultural Science, 27, 64-75.

In Zimbabwe, smallholder cultivation of leaf rape is of considerable importance due to the increasing demand for the leaf vegetable from the rapidly increasing urban populations.  A two-season field trial was conducted in the Agricultural Practice Experimental Plots of the Midlands State University in central Zimbabwe. The objective of the study was to establish selected biometric responses of leaf rape to N fertilizer rates and time to first harvesting. Increasing the rate of N fertilizer application considerably improves the yield of fresh rape leaf; LAI and concentration of leaf nitrate. Delaying rape leaf harvest after N fertilizer side dressing can be used as a strategy to boost rape leaf yield for vegetable markets with high single delivery demand. When harvesting is deliberately delayed after N fertilizer application rape LAI are significantly increased. Delaying the first leaf harvests by 7; 14 and 21 days after N fertilizer side dressing amendments increases the content of nitrate in fresh rape leaves. Fresh rape yield can be more accurately predicted by values of rape LAI. Nitrate concentrations in fresh rape leaf is a poor predictor of rape yield under field conditions.

  1. Anjana, S.U., Iqba, M. (2006): Nitrate accumulation in plants, factors affecting the process, and human health implications: A review. Agronomy for Sustainable Development 27: 45–57. DOI: 10.1051/agro:2006021.
  2. Anjum, A., Gill, S.S., Umar, S., Ahmad, I., Duarte, A.C., Pereira, E. (2012): Improving growth and productivity of Oleiferous Brassicas under changing environment: Significance of N and S nutrition, and underlying mechanisms. Scientific World Journal: 1-12. 2012/657808.
  3. Avice, J.C., Etienne, P. (2014): Leaf senescence and N remobilization efficiency in oilseed rape (Brassica napus L.). JXB 65: 3813–3824. DOI:10.1093/jxb/eru177.
  4. Bahar, N.H., Ishida, F.Y., Weerasinghe, L.K. et al. (2017): Leaf-level photosynthetic capacity in lowland Amazonian and high-elevation Andean tropical moist forests of Peru. New Phytologist. 214: 1002–1018. doi: 10.1111/nph.14079.
  5. Beeves, L., Hageman, R.H. (1983): Uptake reduction of nitrate. Bacteria and higher plants. In: Laudhli A, Bieleski RC, eds. Encyclopedia of Plant Physiology, New Series. 15A: 351-375.
  6. Black, G. G., Hartge, K. H. (1986): Bulk density. Methods of soil analysis. Part 3 ASA Madison, WI.
  7. Bouchet, A.S., Laperche, A., Bissuel-Belaygue, C., Snowdon, R., Nesi, N., Stahl, A. (2016): Nitrogen use efficiency in rapeseed. A review. Agronomy for Sustainable Development 36: 1–20.
  8. Bouyoucos, G. J. (1965): Hydrometer method improved for making particle size analysis of soils. Agronomy Journal 27: 738–741.
  9. Bremner, J.M. (1996): N total. In: Sparks D L, eds. Methods of soil analysis: Part 3. Chemical methods, (Number 5) in the Soil Science Society of America. Book series Soil Science Society of America, Inc., American Society of Agronomy, Inc., Madison, Wisconsin, USA, pp 1085 – 1121.
  10. Brown, J.R., Marshall, C., Smith, G.E. (1999): Nitrate accumulation in vegetable crops as influenced by soil fertility practices. Missouri Agricultural Experiment Station Research Bulletin. 920:1-23.
  11. Cataldo, D.A., Haroon, M., Schrader, L.E., Youngs, V.L. (1975): Rapid colorimetric determination of nitrate in plant tissue by nitration of salicylic acid. Communications in Soil Science and Plant Analysis 6: 71-80.
  12. Chakwizira, E., Johnstone, P., Fletcher, A.L., Meenken, E.D. (2014): Effects of N rate on nitrate – N accumulation in forage kale and rape crops. Grass and Forage Science 70(2): 268-282.
  13. Daubresse, C.M., Vedele, F.D., Deehorgnet, J., Chardon, F., Gaufichon, L., Suzuki, A. (2010): N uptake, assimilation and remobilization in plants: challenges for sustainable and productive agriculture. Annals of Botany. 105 (7): 1141-1157.
  14. De Lannoy, G. (2001): Vegetables: Pumpkins. In: Raemaekers RH (ed) Crop production in tropical Africa. Directory General for International Cooperation (DGIC). Brussels, Belgium, pages 459 - 461. DOI:10.1007/s40003-014-0147-1.
  15. FAO-ISS-ISRIC (1998): World Reference Base for Soil Resources. World Soil Resource Report 84. FAO, Rome.
  16. GenStat (2003): GenStat for Windows (7th Edition) Introduction. Hemel Hempstead: VSN International.
  17. Gonzalez, A., Hyde, E., Sangwan, N., Gilbert, J.A., Viirre, E., Knight, R. (2016): Migraines are correlated with higher levels of nitrate-, nitrite-, and nitric oxide-reducing oral microbes in the American Gut Project Cohort. Systems 1(5): 105-116. 1:e00105–00116. 10.1128/mSystems.00105-16.
  18. Hirel, B., Le Gouis, J., Ney, B., Gallais, A. (2007): The challenge of improving N use efficiency in crop plants: towards a more central role for genetic variability and quantitative genetics within integrated approaches. JXB. 58: 2369–2387. DOI:10.1093/jxb/erm097.
  19. Iammarino, M., Di Taranto, A., Cristino, M. (2014): Monitoring of nitrites and nitrate levels in leafy vegetables (spinach and lettuce): a contribution to risk assessment. Journal of the Science of Food in Agriculture 15:773-8. DOI:10.1002/jsfa.6439.
  20. Johnson, I.R., Thornley, J.H., Frantz, J.M., Bugbee, B. (2010): A model of canopy photosynthesis incorporating protein distribution through the canopy and its acclimation to light, temperature and CO2. Annals of Botany. 106: 735–749. DOI:10.1093/aob/mcq183
  21. Joshipura, K.J., Hu, F.B., Manson, J.E. (2001): The effect of fruit and vegetable intake on risk for coronary heart disease. Annals of Internal Medicine 134:1106–1114. DOI:10.7326/0003-4819-134-12-200106190-00010.
  22. Kuntashula, E., Mafongoya, P.L., Sileshi, G., Lungu, S. (2004): Potential of biomass transfer technologies in sustaining vegetable production in the wetlands (dambos) of eastern Zambia. Experimental Agriculture 40: 37–51.
  23. Kusumi, K., Hirotsuka, S., Shimada, H., Chono, Y., Matsuda, O., Iba, K. (2010): Contribution of chloroplast biogenesis to carbon–nitrogen balance during early leaf development in rice. Journal of Plant Research 123: 617–622. DOI:10.1007/s10265-009-0277-x.
  24. Li, X., Li, Q., Yang, T., Nie, Z., Chen, G., Hu, L. (2016): Responses of plant development, biomass and seed production of direct sown oilseed rape (Brassica napus) to nitrogen application at different stages in Yangtze River Basin. Field Crops Research 194: 12–20.
  25. Maereka, E.K., Madakadze, R.M., Mashingaidze, A.B., Kageler, S., Nyakanda, C. (2007): Effect of N fertilization and timing of harvesting on leaf nitrate content and taste in mustard rape (Brassica juncea L. Czern). Journal of Food Agriculture and Environment 5 (3 & 4):285 - 290.
  26. Masaka, J. & Ndhlovu, S. (2007): The effect of different forms of water hyacinth (Eichhornia crassipes) organic fertilizers on leaf growth rate and yield of rape (Brassica napus). International Journal of Agricultural Research. 2: 254-260. DOI:10.3923/ijar.2007.254.260.
  27. McCall, D., Willumsen, J. (1998): Effects of nitrate, ammonium and chloride application on the yield and nitrate content of soil-grown lettuce. Journal of Horticultural Science and Biotechnology 33:134-5. 14620316.1998.11511036.
  28. Nelson, D. W., Sommers, L. E. (1996): Methods of determination of total organic carbon (TOC) in soils and sediments. In: Page A L ed. Total C, organic C and organic matter. Ecological Risk Assessment Center, Office of Research and Development, US. Environmental Protection Agency, Las Vegas, pp 456-691 461.
  29. Nyakudya, I.W., Jimu, L., Marashe, M., Katsvanga, C.A.T. (2010): Comparative growth and yield responses of rape (Brassica napus L.) to different soil fertility management amendments. Electronic Journal of Environmental, Agricultural and Food Chemistry 9(1): 207-214.
  30. Oldham, C.H. (1999): Brassica crops and allied Cruciferous crops. Biotech Books; Delhi.
  31. Phillips, W.E.J. (2005): Changes in the nitrate and nitrite contents of fresh and processed spinach during storage. J Agric Food Chem. 16 (1): 88-91. DOI: 10.1021/jf60155a012.
  32. Rathke, W.I., Behrens, T., Diepenbrock, W. (2006): Integrated N management strategies to improve seed yield, oil content and N efficiency of winter oilseed rape (Brassica napus L.): A review. Agriculture, Ecosystems & Environment. 117: 2 (3) 80-108.
  33. Rathore, P.S., Manohar, S.S. (1989): Response of mustard to nitrogen and sulphur. Effect of nitrogen and sulphur on ancillary characters and yield of mustard. Indian J Agron. 34(3): 333-336.
  34. Reid, J.B. (2002): Yield response to nutrient supply across a wide range of conditions. Model derivation. Field Crops Research. 77: 161- 171. DOI:10.1016/S0378-4290(02)00087-4.
  35. Roggatz, U., McDonald, A.J., Stadenberg, I., Schurr, U. (1999): Effects of nitrogen deprivation on cell division and expansion in leaves of Ricinus communis L. Plant, Cell and Environment 22: 81–89.
  36. Rossato, L., MacDuff, J.H., Laine, P., Le Deunff, E., Ourry, A. (2002): N storage and remobilization in Brassica napus L. during the growth cycle: effects of methyl jasmonate on nitrate uptake, senescence, growth, and VSP accumulation. Journal of Experimental Botany 53: 1131–1141. 10.1093/jexbot/53.371.1131.
  37. Schjoerring, J.K., Bock, J.G., Gammelvind, L., Jensen, C.R., Mogensen, V.O. (1995): N incorporation and remobilization in different shoot components of field-grown winter oilseed rape (Brassica napus L.) as affected by rate of N application and irrigation. Plant and Soil 177: 255–264. DOI:10.1080/01904167.2010.489983.
  38. Singh, T., Dahiya, K.S., Sidhu, M.S. (2006): Effect of genotype, seedling age and row spacing on performance of transplanted African mustard (Brassica carinata) under late-sown condition. Indian Journal of Agronomy 51(3): 221-224.
  39. Stopes, C., Woodward, L., Forde, G., Vogtmann, H. (1989): Effect of composted farmyard manure and a compound fertilizer on yield and nitrate accumulation in three summer lettuce cultivars grown in an organic system. Agriculture Ecosystems & Environment 27(1-4):555 - 560. DOI:10.1016/0167-8809(89)90116-3.
  40. Terry, P., Terry, J.B., Wolk, A. (2001): Fruit and vegetable consumption in the prevention of cancer: An update. Journal of Internal Medicine 250: 280–290. 10.1111/j.1365-2796.2001.00886.x.
  41. Vincent, V., Thomas, R.G. (1960): An Agricultural Survey of Southern Rhodesia, Part I Agro-Ecological Survey, Government Printers, Salisbury.