Enhancing African leafy vegetable productivity and nutrient levels through manure and fertilizer in Kitui County, Kenya
Authors
View
Keywords
License
Copyright (c) 2026 International Journal of Horticultural Science

This work is licensed under a Creative Commons Attribution 4.0 International License.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
How To Cite
Abstract
African Leafy Vegetables (ALVs) are crucial components of diet globally due to their significant nutritional value. We conducted a field experiment in October 2022 to assess the effect of fertilizer application rates on the growth and nutritive value of four ALVs (Solanum nigrum, Cleome gynandra, Amaranthus hybridus, and Vigna unguiculata) at South Eastern Kenya University (SEKU) Teaching and Research Farm. We collected data through a randomized complete block experimental design that comprised four treatments: manure alone (M), fertilizer alone (F), manure + fertilizer (M+F), and a control (C). Once the planted vegetable seeds had been established, we counted their leaves on a weekly basis and averaged after a month. At the end of the experiment, the ALVs leaves were harvested. They were analyzed for retinol, a precursor of vitamin A, iron, calcium and zinc. It was found that manure and fertilizer treatments significantly increased the number of ALVs leaves. Results also showed that the Solanum nigrum had the highest number of leaves at 118.5% in M+F treatment as compared to the control. Across all vegetables, a combined treatment (M+F) significantly affected the retinol mean (P=0.0063) while crop type and the interaction between crop type and the treatment had no statistically meaningful impact on any nutrient. This suggests that fertilization effects on retinol are independent of crop type. These results highlight the importance of soil fertilization in influencing vitamin A levels in ALVs.
References
- Abebe, T. G., Tamtam, M. R., Abebe, A. A., Abtemariam, K. A., Shigut, T. G., Dejen, Y. A., Haile, E. G. (2022): Growing use and impacts of chemical fertilizers and assessing alternative organic fertilizer sources in Ethiopia. Applied and Environmental Soil Science. 2022(1): 4738416. https://doi.org/10.1155/2022/4738416
- Adedibu, P. A., Animasaun, D. A., Tekutyeva, L., Son, O., Abubakar, M. A., Adamu, U. M., Balabanova, L., Yugay, Y., Shkryl, Y. (2024): Variability in Morphological, Biochemical, and Proximate Yield Composition among Predominant Amaranthus hybridus Cultivars in South-West Nigeria. Horticulturae. 10(5): 461. https://doi.org/10.3390/horticulturae10050461
- Adediran, J. A., Taiwo, L. B., Akande, M. O., Sobulo, R. A., Idowu, O. J. (2005): Application of organic and inorganic fertilizer for sustainable maize and cowpea yields in Nigeria. Journal of plant nutrition. 27(7): 1163-1181. https://doi.org/10.1081/PLN-120038542
- Adjogboto, A., Likpètè, D. D., Sossa-Vihotogbé, C. N. A., Akponikpè, P. B. I., Djènontin, A. J., Baco, M. N., Diogo, V.C., Agbossou, K. E. (2023): Yield response of African leafy vegetables to combined manure and urea microdosing in West Africa. Biotechnology, Agronomy and Society and Environment. 27(2):74-86. https://doi.org/10.25518/1780-4507.20248
- Aparna, T. (2011): Quality evaluation in organic amaranthus (Amaranthus tricolor L.). Vellanikkara, India, PhD Thesis. Available online at: https://krishikosh.egranth.ac.in/items/619d9891-a147-4bc6-afa3-453b6aae9dfa (Accessed on 5th, August 2025).
- Arisha, H. M. E., Gad, A. A., Younes, S. E. (2003): Response of some pepper cultivars to organic and mineral nitrogen fertilizers under sandy soil conditions. Zagazig Journal of Agricultural Research (Egypt). 30(5). http://nile.enal.sci.eg/EALE/2003/ZJAR/3003/5/1875.pdf
- Binder, E.V. (2019): Alternative Food and Feed Products. In Proceedings of the First FAO/WHO/AU International Food Safety Conference, Addis Ababa, Ethiopia, 12–13 February 2019; IFSC-1/19/TS2.5. Available online at: https://www.who.int/docs/default-source/resources/bp-alternative-food-and-feed-products.pdf (Accessed on 10th, August 2025).
- Bittelli, M. (2011): Measuring Soil Water Content: A Review. HortTechnology. 21(3): 293-300. https://doi.org/10.21273/HORTTECH.21.3.293
- Black, R. E. (2014): Global distribution and disease burden related to micronutrient deficiencies. In Nestle Nutrition Institute Workshop Series.78: 21-8). https://doi.org/10.1159/000354932
- Boskovic-Rakocevic, L., Pavlovic, R., Zdravkovic, J., Zdravkovic, M., Pavlovic, N., Djuric, M. (2012): Effect of nitrogen fertilization on carrot quality. African Journal of Agricultural Research. 7(18):2884-2900. https://doi.org/10.5897/AJAR11.1652
- Bukomarhe, C. B., Kimwemwe, P. K., Githiri, S. M., Mamati, E. G., Kimani, W., Mutai, C., Nganga, F., Nguezet, P. D., Mignouna, J., Civava, R. M., Fofana, M. (2023): Association mapping of candidate genes associated with iron and zinc content in rice (Oryza sativa L.) grains. Genes. 14(9):1815. https://doi.org/10.3390/genes14091815
- Centers for Disease Control and Prevention [CDC]. (2008): Laboratory Procedure Manual: Vitamin A, Vitamin E, and Carotenoids in Serum (NHANES 2005–2006). National Center for Environmental Health, Division of Laboratory Sciences. Available online at: https://wwwn.cdc.gov/nchs/data/nhanes/2005-2006/labmethods/vitaec_met_d.pdf (accessed on 5th, August 2025).
- Chang Y., Liu H., Liu M., Liao X., Sahu S. K., Fu Y., Song, B., Cheng, S., Kariba, R., Muthemba, S., Hendre, P. S., Mayes, S., Ho, W.K., Yssel, A. E.J., Kendabie, P., Wang, S., Li, L., Muchugi, A., Jamnadass, R., … Liu, X. (2018): The draft genomes of five agriculturally important African orphan crops. GigaScience. 8: 1–16. https://doi.org/10.1093/gigascience/giy152
- Ciosek, Ż., Kot, K., Kosik-Bogacka, D., Łanocha-Arendarczyk, N., Rotter, I. (2021): The effects of calcium, magnesium, phosphorus, fluoride, and lead on bone tissue. Biomolecules. 11(4): 506. https://doi.org/10.3390/biom11040506
- Eric, M., Najma, D., Samita, N., Karanja, R. (2024): Impacts of selected Climate Smart Agricultural Practices on African Indigenous Vegetables in Kenyan drylands. African Phytosanitary Journal. 4(1): 66-85. https://doi.org/10.52855/UWBW9156
- Fageria, N. K. (2016): The use of nutrients in crop plants. CRC press.
- Failloux, N., Bonnet, I., Perrier, E., Baron, M. H. (2004): Effects of light, oxygen and concentration on vitamin A1. Journal of Raman Spectroscopy. 35(2): 140-147. https://doi.org/10.1002/jrs.1116
- FAO. IFAD. UNICEF. WFP. WHO. (2021): The state of food security and nutrition in the world 2021. transforming food systems for food security, improved nutrition and affordable healthy diets for all (Rome, Italy). Available online at: https://www.sidalc.net/search/Record/dig-fao-it-20.500.14283-cb4474en/Description (Accessed on 3rd, August 2025).
- Fonge, B. A., Bechem, E. E., Awo, E. M. (2016): Fertilizer rate on growth, yield, and nutrient concentration of leafy vegetables. International Journal of Vegetable Science. 22(3): 274-288. https://doi.org/10.1080/19315260.2015.1005726
- Fritz, D., Habben, J. (1973): Influence of fertilization on the quality of vegetable particular for processing. Acta horticulturae. 29: 349-360. https://doi.org/10.17660/ActaHortic.1973.29.27
- Gonye, E., Kujeke, G. T., Edziwa, X., Ncube, A., Masekesa, R. T., Icishahayo, D., Matikiti, A., Chabata, I. (2017): Field performance of spider plant (Cleome Gynandra L) under different agronomic practices. African Journal of food, agriculture, nutrition and development. 17(3): 12179-12197. https://doi.org/10.18697/ajfand.79.15985
- Hutchinson, M. J. (2011): The effect of farmyard manure and calcium ammonium nitrate fertilisers on micronutrient density (iron, zinc, manganese, calcium and potassium) and seed yields of solanium villosum (black nightshade) and cleome gynandra (cat whiskers) on uetric nitisol. Journal of Agriculture, Science and Technology. 13(1): 35-52.
- Imathiu, S. (2021): Indigenous African leafy vegetables for food and nutrition security. Journal of Food Security. 9: 115-125. http://DOI:10.12691/jfs-9-3-4
- Karuma, A., Mtakwa, P., Amuri, N., Gachene, C. K., Gicheru, P. (2015): Tillage and cropping effects on soil water dynamics and crop yields in Mwala District, Kenya. Available onlime at: https://www.researchgate.net/publication/281522599_Tillage_and_Cropping_Effects_on_Soil_Water_Dynamics_and_Crop_Yields_in_Mwala_District_Kenya (Accessed on 3rd, August 2025).
- Kihara, J., Bolo, P., Kinyua, M., Rurinda, J., Piikki, K. (2020): Micronutrient deficiencies in African soils and the human nutritional nexus: Opportunities with staple crops. Environmental geochemistry and health. 42(9): 3015-3033. https://doi.org/10.1007/s10653-019-00499-w
- Kiio, J., Ochola, S., Nduati, R., Kuria, E., Mathenge, S., Okoth, J. (2022): Bioequivalence of micronutrient powders to Corn-soy Blend on serum zinc concentration of children (6–36 months) with Moderate Acute Malnutrition in Thika urban slums, Kenya: A cluster-randomized controlled trial. Plos one. 17(9): e0274870. https://doi.org/10.1371/journal.pone.0274870
- Kipkorir, P., Wakhungu, H., Ali, J., Mulango, E., Nyakundi, G., Kiplagat, I., Njeri, M., Wachilonga, J., Macharia, E. (2024): Food and Nutrition Security in Kenya: Embedding Nutrition Element within the Four Pillars of Food Security in the Counties. Available online at: http://dx.doi.org/10.13140/RG.2.2.21658.66246 (Accessed on 6th, August 2025).
- Kipkosgei, L. K., Akundabweni, L. S. M., Hutchinson, M. J. (2003): The effect of farmyard manure and nitrogen fertilizer on vegetative growth, leaf yield and quality attributes of Solanum villosum (Black nightshade) in Keiyo District, Rift Valley. African Crop Science Conference Proceedings. 6: 514–518. http://hdl.handle.net/11295/38279
- Kitavi, E. K., Ndung’u, C. K., Mwangi, M. (2024): The influence of intensive agriculture on soil properties and nutrient availability in Kauwi and Zombe wards of Kitui County, Kenya. East African Journal of Agriculture and Biotechnology. 7(1): 1-12.
- Liceaga, A. M., Aguilar-Toalá, J. E., Vallejo-Cordoba, B., González-Córdova, A. F., Hernández-Mendoza, A. (2022): Insects as an alternative protein source. Annual Review of Food Science and Technology. 13(1): 19-34. https://doi.org/10.1146/annurev-food-052720-112443
- Lisiewska, Z., Kmiecik, W. (1996): Effects of level of nitrogen fertilizer, processing conditions and period of storage of frozen broccoli and cauliflower on vitamin C retention. Food Chemistry. 57(2): 267-270. https://doi.org/10.1016/0308-8146(95)00218-9
- Mabhaudhi, T., Chimonyo, V. G. P., Hlahla, S., Massawe, F., Mayes, S., Nhamo, L., Modi, A. T. (2019): Prospects of orphan crops in climate change. Planta. 250: 695-708. https://doi.org/10.1007/s00425-019-03129-y
- Makus, D. J. (1986): Vegetable amaranth response to nitrogen fertility under moderately low and very low residual soil phosphorus levels. HortScience. 21(3):697. http://worldveg.tind.io/record/19073
- Mani, P. K. (2011): Manure for vegetable production. Chapter 11. Available online at: https://www.researchgate.net/publication/313652054_Manure_for_vegetable_production (Accessed on 7th, August 2025).
- Manono, B. O. (2016): Carbon dioxide, nitrous oxide and methane emissions from the Waimate District (New Zealand) pasture soils as influenced by irrigation, effluent dispersal and earthworms. Cogent Environmental Science. 2(1): 1256564. https://doi.org/10.1080/23311843.2016.1256564
- Manono, B. O., Moller, H., Benge, J., Carey, P., Lucock, D., Manhire, J. (2019): Assessment of soil properties and earthworms in organic and conventional farming systems after seven years of dairy farm conversions in New Zealand. Agroecology and Sustainable Food Systems. 43(6): 678-704. https://doi.org/10.1080/21683565.2019.1570997
- Manono, B. O., Moller, H., Morgan, R. (2016): Effects of irrigation, dairy effluent dispersal and stocking on soil properties of the Waimate District, New Zealand. Geoderma regional. 7(1): 59-66. https://doi.org/10.1016/j.geodrs.2016.01.002
- Maseko, I., Mabhaudhi, T., Tesfay, S., Araya, H. T., Fezzehazion, M., Plooy, C. P. D. (2017): African leafy vegetables: A review of status, production and utilization in South Africa. Sustainability. 10(1):16. https://doi.org/10.3390/su10010016
- Mashamaite, C. V., Manyevere, A., Chakauya, E. (2022): Cleome gynandra: A wonder climate-smart plant for nutritional security for millions in semi-arid areas. Frontiers in Plant Science. 13: 1003080. https://doi.org/10.3389/fpls.2022.1003080
- Matthewman, M. C., Costa-Pinto, R. (2023): Macronutrients, minerals, vitamins and energy. Anaesthesia & Intensive Care Medicine. 24(2): 134-138. https://doi.org/10.1016/j.mpaic.2022.12.009
- Mavengahama, S., Ogunlela, V. B., Mariga, I. K. (2006): Response of paprika (Capsicum annuum L.) to basal fertilizer application and ammonium nitrate topdressing in semi-arid Zimbabwe. Crop Research. 32, 421–429.
- Ministry of Health. (2011): The Kenya national micronutrient survey. Nairobi, Kenya. Available online at: https://www.nutritionhealth.or.ke/wp-content/uploads/Downloads/The%20Kenya%20National%20Micronutrient%20Survey%202011.pdf (Accessed on 5th, August 2025).
- Mishra, S. S., Moharana, S. K., Dash, M. R. (2011): Review on Cleome gynandra. International Journal of Research in Pharmacy and Chemistry. 1(3): 681-689. http://www.ijrpc.com/
- Motladi, M. (2024): Comparative effects of bioslurry and chemical fertilizer on soil properties and performance of spider plant (Cleome gynandra L). (Botswana University of Agriculture and Natural Resources, Botswana). Available online at: https://researchhub.buan.ac.bw/handle/13049/771 (Accessed on 5th, August 2025).
- Moyo, M., Aremu, A. O. (2022): Nutritional, phytochemical and diverse health-promoting qualities of Cleome gynandra. Critical Reviews in Food Science and Nutrition. 62(13): 3535-3552. https://doi.org/10.1080/10408398.2020.1867055
- Mozafar, A. (2008): Nitrogen fertilizers and the amount of vitamins in plants: A review. Journal of plant nutrition. 16(12): 2479-2506. https://doi.org/10.1080/01904169309364698
- Murage, E. N. (1990): The effect of nitrogen rates on growth, leaf yield and nitrite quality of black night shade. Nairobi, Kenya: University of Nairobi, MSc Thesis. Available online at https://erepository.uonbi.ac.ke/handle/11295/22094 (Accessed on 9th, August 2025).
- Muthama, E., Najma, D., Samita, N., Karanja, R. (2024): Impacts of selected Climate Smart Agricultural Practices on African Indigenous Vegetables in Kenyan drylands. African Phytosanitary Journal. 4(1): 66-85. https://doi.org/10.52855/UWBW9156
- Ng'etich, O. K., Aguyoh, J. N., Ogweno, J. O. (2014): The Effect of integrated application of farmyard manure and calcium ammonium nitrate on growth and yield attributes of African nightshade (Solanum scabrum Mill.). Asian Journal of Agriculture and Food Sciences. 2(2).
- Nyonje, W. A., Makokha, A. O., Abukutsa-Onyango, M. O. (2014): Anti-nutrient, phytochemical and antiradical evaluation of 10 amaranth (Amaranthus spp.) varieties before and after flowering. Journal of Agricultural Science. 6(4): 68. http://dx.doi.org/10.5539/jas.v6n4p68
- Onyango, C. M., Harbinson, J., Imungi, J. K., Shibairo, S. S., Van Kooten, O. (2012): Influence of organic and mineral fertilization on germination, leaf nitrogen, nitrate accumulation and yield of vegetable amaranth. Journal of plant nutrition. 35(3): 342-365. https://doi.org/10.1080/01904167.2012.639917
- Onyango, C. M., Harbinson, J., Imungi, J. K., Van Kooten, O. (2011): Influence of maturity at harvest, N fertiliser and postharvest storage on dry matter, ascorbic acid and β-carotene contents of vegetable amaranth (Amaranthus hypochondriacus). International Journal of Postharvest Technology and Innovation. 2(2): 180-196. https://doi.org/10.1504/IJPTI.2011.04104
- Oremo, F. O. (2013): Small-scale farmers’ perceptions and adaptation measures to climate change in Kitui. Nairobi, Kenya: University of Nairobi, MSc Thesis. Available online at: http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/59852 (Accessed on 3rd, August 2025).
- Owidhi, M. A. (2020): Climate Change and Agriculture in Kenya. In Sustainable Bioresource Management. (pp. 21-28). Apple Academic Press.
- Pareek, N. (2017): Climate change impact on soils: Adaptation and mitigation. MOJ Ecology & Environmental Science. 2(3). http://dx.doi.org/10.15406/mojes.2017.02.00026
- Ramankutty, N., Mehrabi, Z., Waha, K., Jarvis, L., Kremen, C., Herrero, M., Rieseberg, L. H. (2018): Trends in global agricultural land use: Implications for environmental health and food security. Annual review of plant biology. 69(1): 789-815. https://doi.org/10.1146/annurev-arplant-042817-040256
- Roe, N. E., Cornforth, G. C. (2000): Effects of dairy lot scrapings and composted dairy manure on growth, yield, and profit potential of double cropped vegetables. Compost Science & Utilization. 8(4): 320-327. https://doi.org/10.1080/1065657X.2000.10702005
- Sangija, F., Martin, H., Matemu, A. (2021): African nightshades (Solanum nigrum complex): The potential contribution to human nutrition and livelihoods in sub‐Saharan Africa. Comprehensive Reviews in Food Science and Food Safety. 20(4): 3284-3318. https://doi.org/10.1111/1541-4337.12756
- Sardar, H., Hadi, F., Alam, W., Halawani, I. F., Alzahrani, F. M., Saleem, R. A., Cerqua, I., Khan, H., Capasso, R. (2024): Unveiling the therapeutic and nutritious potential of Vigna unguiculata in line with its phytochemistry. Heliyon. 10 (18): e37911. https://doi.org/10.1016/j.heliyon.2024.e37911
- Sawe, C. J., Kogi-Makau, W., Ettyang, G. A., Kimamo, C.O. (2021): Tripartite of malnutrition: Co-existence of underweight, overweight and micronutrient deficiency among children in Kisumu County, Kenya. https://doi.org/10.21203/rs.3.rs-209539/v1
- Stadlmayr, B., Trübswasser, U., McMullin, S., Karanja, A., Wurzinger, M., Hundscheid, L., Riefler, P., Lemke, S., Brouwer, I. D., Sommer, I. (2023): Factors affecting fruit and vegetable consumption and purchase behavior of adults in sub-Saharan Africa: A rapid review. Frontiers in Nutrition. 10: 1113013. https://doi.org/10.3389/fnut.2023.1113013
- Theola, J., Andriastuti, M. (2025): Neurodevelopmental impairments as long-term effects of iron deficiency in early childhood: A systematic review. Balkan Medical Journal. 42(2):108. https://doi.org/10.4274/balkanmedj.galenos.2025.2024-11-24
- Theuri, A. W., Makokha, A., Kyallo, F., Gichure, J. N. (2023): Effect of using mobile phone communication on dietary management of Type 2 Diabetes Mellitus patients in Kenya. Journal of Diabetes & Metabolic Disorders. 22(1): 367-374. https://doi.org/10.1007/s40200-022-01153-6
- Turan, M., Sevimli, F. (2005): Influence of different nitrogen sources and levels on ion content of cabbage (Brassica oleracea var. capitate). New Zealand Journal of Crop and Horticultural Science. 33(3): 241-249. https://doi.org/10.1080/01140671.2005.9514356
- Ullah, M. S., Islam, M. S., Islam, M. A., Haque, T. (2008): Effects of organic manures and chemical fertilizers on the yield of brinjal and soil properties. Journal of the Bangladesh Agricultural University. 6(2). https://doi.org/10.22004/ag.econ.208301
- United Nations [UN]. (2019): World population prospects 2019: Data booklet. Statistical Papers-United Nations, Population and vital Statistics Report. Available online at: https://population.un.org/wpp/assets/Files/WPP2019_Highlights.pdf (Accessed on 7th, August 2025).
- Van Averbeke, W., Chabalala, M. P., Okorogbona, A. O. M., Ramusandiwa, T. D., Azeez, J. O., Slabbert, M. M. (2012): Plant nutrient requirements of African leafy vegetables. Nutritional value and water use of African leafy vegetables for improved livelihoods. 173-209. Available online at: https://www.wrc.org.za/wp-content/uploads/mdocs/TT%20535-12.pdf (Accessed on 5th, August 2025).
- Waselkov, K. E., Boleda, A. S., Olsen, K. M. (2018): A phylogeny of the genus Amaranthus (Amaranthaceae) based on several low-copy nuclear loci and chloroplast regions. Systematic Botany. 43(2): 439-458. https://doi.org/10.1600/036364418X697193
- Weston, L. A., Barth, M. M. (1997): Preharvest factors affecting postharvest quality of vegetables. HortScience. 32(5): 812-816.
- World Health Organization [WHO (2025]: Vitamin A deficiency. Available online at: https://www.who.int/data/nutrition/nlis/info/vitamin-a-deficiency (Accessed on 29th, July 2025).
https://doi.org/10.31421/ijhs/32/2026/15993