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Definition antioxidant activity of selenium-enriched food sprout, as well as their microbiological analysis
25-30Views:255In this present study, we prepared selenium-enriched food sprouts, where the antioxidant capacity was analysed, we also determined their microbiological status. We took into account the fact, we choose micronutrients to our treatment, that selenium can be delivered to the body by a small amount with the most widely consumed food.
We focused during our research to determine that the increasing concentrations of selenium treatment, in which we used sprouts, knowing fully well that it has an impact on aboriginal antioxidant capacity of sprouts, which is mainly due to high vitamin content of sprouts.
Furthermore, we think it is important to make microbiological analysis, because germination conditions, for example temperature, pH, all this will create an ideal environment for the growth of microorganisms. So we had goal to determine, how the used selenium concentration affect the total plate count, coliform bacteria count and Staphylococcus aureus count of sprouts.
We determined the aboriginal water-soluble and lipid-soluble antioxidant capacity of sprout with the PHOTOCHEM chemiluminometer and we applied pour plate technique for the mapping of the mycrobiological state of sprouts.
Experimental results are evaluated, we state that increasing concentrations of selenite or selenate treatment, is primarily water-soluble antioxidant capacity of sprouts was affected. The water-soluble antioxidant capacity of wheat sprout was much higher than the measured values in pea sprout, this may be linked to what we measured. That is much higher ascorbic acid content in case of wheat sprout, which is well known as one of the most important antioxidant properties compounds of wheat sprout.
We conclude from the results of the microbiological, that the highest concentrations of selenite or selenate treatment has a relative significant anti-microbial effect in case of wheat sprouts. Coliform and total plate count showed no clear decreasing tendency, although the values of treatments in both cases obtained were below the control values.
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Selenium speciation analysis of selenium-enriched food sprouts
23-28Views:218In this present study, we prepared selenium-enriched pea and wheat sprouts. During our research we aimed not only to measure the total selenium content of the sprouts but to identify different selenium species.
Scientifical researches show why the analytical examination of different selenium (Se) species is necessary: consumption of all kind of Se-species is useful for a person who suffers in selenium deficit, while there is significant difference between effects of different Se-species on person, in whose body the Se-level is just satisfactory. Biological availability, capitalization, accumulation, toxicity of Se-species are different, but the main difference was manifested in the anti-cancer effect of selenium.
During our research selenium was used in form of sodium selenite and sodium selenate, the concentration of the solutions used for germination was 10 mg dm-3. Control treatment meant germination in distilled water. Total selenium content of sprout samples was measured after microwave digestion by inductively coupled plasma mass spectrometry (ICP-MS). Different extraction solvents were applied during sample preparation in order to separate different Se-species (0.1 M and 0.2 M HCl or 10 mM citric acid buffer). We wanted the following question to be answered: Which extraction solvent resulted the best extraction efficiency? Selenium speciation analysis of sprout sample extracts was performed by high performance liquid chromatography with anion exchange column, detection of selenium species was performed by ICP-MS.
Evaluating our experimental results we have been found that significant amount of selenium of inorganic forms used during germination transformed into organic selenium compounds. There was difference between the amount of Se-species in pea and wheat sprouts and selenium uptake and repartition of selenium species were depended on Se-form used during germination. In addition the chromatogram analysis made us clear as well, that the citric acid solvent proved to be the most effective extraction solvent during sample preparation int he view of organic Se species.
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Changes of the fatty acid composition of sprouts during germination
89-92Views:140During our research we studied the fat content and fatty acid composition during the germination and sprouting periods of the most important sprouts: wheat, lentil, alfalfa, radish and sunflower seed. In this article we present our research results during this sprouting study. The concentration of the saturated fatty acids (palmitic acid, stearic acid) decreased, the concentration of the unsaturated fatty acids increased during germination, but the tendency was not so high than was published in the literature.
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The examination of roe deer feeding on agricultural and woody habitats in winter
23-26Views:123sorting because the micro organisms, which help the digestion of high fibre plants, are missing in his stomack, that is why they are mostly called „concentrate selectors” (Hoffmann, 1985, 1988, 1989).
These animals should mostly eat easily digestable plants with high nutrition level (pulses, buds, sprouts and flowers), and they are able to do this sorting because of their mouth size. In winter there is a lack of these plants, so the high selectivity occurs only when the feed is in abbundance.
Examining the amount and quality of vegetation available on the habitat of roe deer we can identify the species which can satisfy their feed demand. It is known, that roe deer as other large ruminants, from the plant abundance prefer certain plants and plant parts while there are others which are avoided. The identification of the eaten species and the rate of their occurrence in the feed is the first step to become acquainted with the interaction between animal and the surroundings.
On the examined territories there was significant difference between the disembowelled body masses. The does which come from the hunt Nagyszénás weighed 4-5 kilos more than the does from the Csongrád territory. The main feed components were present in different rate in the samples of the territories, in the Nagyszénás samples grain, dicotyledonous herbacous plants were dominant, and were eaten more. The high level of grain in the feed result in better condition and larger body mass. -
Analysis of the main parameters of spring and summer food compound of roe deer (Capreolus capreolus L.) on the two hunting grounds of the Alföld plain
7-10Views:140It is widespread that roe deer are very choosy. It needs this sorting because the micro organisms, which help the digestion of high fibre plants, are missing in its stomach that is why they are mostly called „concentrate selectors” (Hoffmann, 1985, 1988, 1989).
These animals should mostly eat easily digestible plants with high nutrition level (pulses, buds, sprouts and flowers), and they are able to do this sorting because of their small mouth size. In winter there is a lack of these plants, so the high selectivity occurs only when the feed is in abundance.
Examining the amount and quality of vegetation available on the habitat of roe deer we can identify the species which can satisfy their feed demand. It is known, that roe deer as other large ruminants, from the plant abundance prefer certain plants and plant parts while there are others which are avoided. The identification of the eaten species and the rate of their occurrence in the feed is the first step to become acquainted with the
interaction between animal and the surroundings. -
Examination of the main parameters of roe deer feed compound on two territories
13-16Views:112It is widespread that roe deer are very choosy. He needs this sorting because the micro organizms, which help the digestion of high fibre plants, are missing in his stomack, that is why they are mostly called „concentrate selectors” Hoffmann, 1985, 1988, 1989).
These animals should mostly eat easily digestable plants with high nutrition level (pulses, buds, sprouts and flowers), and they are able to do this sorting because of their small mouth size. In winter there is a lack of these plants, so the high selectivity occurs only when the feed is in abundance.
Examining the amount and quality of vegetation available on the habitat of roe deer we can identify the species which can satisfy their feed demand. It is known, that roe deer as other large ruminants, from the plant abundance prefer certain plants and plant parts while there are others which are avoided. The identification of the eaten species and the rate of their occurance in the feed is the first step to become acquainted with the interaction between animal and the surroundings.