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Seed biology and possibility of improvement of seed germination capacity on Virginia mallow (Sida hermaphrodita L. Rusby)
71-76Views:238Sida hermephrodita or Virginia mallow is a perspective perennial herb in the Malvaceae family able to yield a biomass crop through the last two decades. Additionally, the plants have a lot of uses and benefits for instance it can use as a fodder crop, honey crop, ornamental plant in public gardens. It has favourable features like fast growing and resistance against the disease and climatic fluctuations, etc. Sida is in the beginning phase of domestication therefore it has a serious disadvantage: the low and slow germination as a big part of wild plants. Due to the expressly low germination percent the need of seed showing of driller is should tenfold, 200 thousand seeds/acre instead of 10–20 thousand what is not available and expensive Therefore practical purposes of our research of seed physiology was to increase the seed germination percent in a available, basically wild Sida population. In the first stage of our experiments we examined two factors relating to seed germination percent and seed germination power during our research: the influence of hot water treatment and the effect of exogenous or endogenous infection of seed. However, in our germination tests, utilizing scarified seeds with hot water (65 oC, 80 oC, 90 oC), from 29,3% to 46% germinated from those samples which were collected from the population of Sida hermaphrodita in Debrecen. The average germination for all season was 5–10% without treatment and rinsed using hot water up to almost 50%. When physically scarified used, the oldest seeds showed the best germination (46%) after the hot water operation in spite of the previous studies. We discovered that apparently there are close relationship between the seed fresh weight or water uptake capability and the percentage of infection. Following these recognition we modified our technique,in such a way that we fractionated the seeds based on their fresh weight/or relative density before we carried out the treatment. When we filtered the floating seeds on the surface of water, the hot water treatment was performed considerably better on the sunk seeds after separation. Therefore, by this special priming process we were able to reach 80% germination capacity of Virgina mallow seeds under laboratory conditions (26 oC without illumination).
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Germination and sugar content alteration in maize grain caused by Fusarium contamination
42-44Views:130Healthy and Fusarium affected ears were collected in Fészerlak, Somogy County at the end of vegetation cycle of maize (25-30 August). Each pattern contains 25-25 ears. We compared the samples on the basis of visual image of Fusarium affection. The ears were shelled and two 0,5 kg samples were formed: healthy and Fusarium contaminated. After surface sterilisation the uniform sized seeds were soaked in sterile distilled water for 24 hours and there were germinated for 7 days. The α-amylase activity was measured with Phadebas- α-amylase test. Seeds were extracted one by one three times under reflux using 10 cm3 boiling water for 15 minutes. During our investigation germinating activity was detected to measure glucose, fructose, sucrose content and α-amylase activity. In the first seven days of germination the highest values were detected in control seeds followed by the affected seeds. Our results clearly show that stress conditions applied altered not only the saccharide content but decreased their germinating activity as well in the case of maize grain.
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Application of GIS, precision agriculture and unplugging cultivation in plant breeding of Karcag
49-56Views:309In the last two decades, the prevailing ecological conditions and climate change have caused negative effects. Therefore, a paradigm shift is needed in the field growing of plants. The latest inventions, digital technologies, precision cultivation are not enough, the mentality of the farmers is more important. For this reason, not only big financial sacrifices, but adequate receptivity are needed on behalf of farmers. Adequate skills and continuous self-education are necessary. The yield of plant growing farms is determined by ecological conditions to a 40% extent, genetic background of the seed has a 30% share and the applied agricultural technology has a proportion of 30%. In different agroecological conditions, bred varieties of plants have bigger tolerance to unfavorable factors of the regions and significant yield stability. Farmers, who buy and sow seeds, can only contribute to the genetic potential of the seeds with cultivation technology. Plant breeding provides stable genetic background and good quality seeds. Breeding activity – choosing variety proposants, breeding them, selection work, classical breeding process for 8-10 years – must create new landraces, which can produce balance, high yield and have good quality parameters in extreme ecological conditions, yearly excursion and have higher tolerance to unfavorable factors of the region giving significant production stability for farmers. In Karcag GIS technology, precision cultivation elements and soil-friendly agrotechnical methods have been introduced which largely support the aims of breeding and can also provide optimal cultivation conditions in extreme years. Because of the specificity of breeding the main aim is not only to increase yield but to provide harmonic growing for bred materials, to decrease the number and the cost of cultivation and to be punctual. In this study, applied new methods and technologies are introduced.
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Assessment of electrical conductivity and germinability of groundnut genotype seeds
5-10Views:496Seed quality affects crop establishment and productivity. In addition, the use of good-quality seed is an essential prerequisite for sustainable crop production including groundnuts. Assessing germinability and electrical conductivity provides early evidence of the production potential of a given crop variety or genotype. Therefore, this study assessed the germinability and electrical conductivity of seeds of three groundnut genotypes. A laboratory experiment arranged in a Completely Randomized Design (CRD), replicated three times, was conducted at the Faculty of Agriculture, Kyambogo University, in 2020. Seeds of Igola, Serenut 1, and Serenut 2 groundnut varieties were tested, and data was collected on germination percentage and electrical conductivity. Analysis of variance (ANOVA) was performed using GenStat and means were separated using the least significant difference test at a 5% probability level. Germination percentage and electrical conductivity significantly (p<0.05) differed among the groundnut varieties, with Igola recording the highest germination percentage, followed by Serenut 1, and the lowest was in Serenut 2. The highest electrical conductivity was recorded in Serenut 1 and the lowest in Igola. Since Igola had one of the lowest electrical conductivity and the highest germination percentage, it was concluded that Igola genotype retained higher quality attributes.
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The influence of crop rotation and nutrition regime in winter wheat on the phytomass remains and the deposits of weed seeds remained in the ground
10-13Views:195An important problem refers to soil weeds seed bank with special reference to brown luvic soils and relationship between vegetal remains
(roots + stub) and nutrition regime – crop rotation plant. A great number of seeds can be found in first 20 centimeters of soil (plowing
layer). This fact causes a high weeds density, which has a negative effect on the cultivated plants leading to partial or total crop loss. -
Effect of sowing time and planting space on change of yield and protein content of white lupine (Lupinus albus L.)
85-89Views:257The hinge of the lupine crop cultivation technology is the sowing time and the determination of the breeding area. According to the literature the early sowing (as soon as possible to go to the soil) and larger growing area is recommended for seed production. Based on the results of our 2 years experiment, the effect of sowing time and different spacing is important on yield of white lupine, and the effect of the sowing time is more significant. A decrease in the yield was observed after later sowings. The yield and the rate of its decreasing was varied in one year and among different years depending on wheather condition and sowing time.
The role of sowing time is significant for protein content of seeds. The average protein content decreased due to the later sowings in 2014, while 2015 the protein content of seeds in early sowing time and of plants sowed 4 weeks later was similar. We measured less protein content in the 2. sowing time sowed 2 weeks later than the first one. In the studied years the average protein content of seeds from different row spaces were not different.
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Study of drought stress correlation on yield and yield components of maize cultivars (Zea mays L.)
67-73Views:514This article was investigated to study the correlation and analysis of drought stress regression on maize cultivars' yield and components. The variance analysis results showed a significant difference between drought stress levels in terms of plant height, total dry weight and number of seeds per row, the total weight of cob, grain yield, harvest index, stem diameter, and cob weight with protective leave. Also, there was a significant difference in ear weight without protective leaves, ear diameter, ear length, plant weight, 100-seed weight, and seed per ear on hybrid treatments. There were statistically significant differences between cultivars in plant height, leaf area, ear diameter, ear length, number of seeds per row, number of seeds per ear, the total weight of cob wood, 100-seed weight, harvest index, plant dry weight. The results of the correlation of traits for the mean levels of drought stress showed a positive and significant correlation between plant yield and plant height, seed per row, ear length and weight of 5 pieces of wood and also with a total weight of cob wood, ear weight with bark showed the highest correlation. There is a significant correlation between leaf area and stem diameter, plant weight, total dry weight at the probability level of 0.05. Correlation coefficients between traits in non-stress conditions showed a positive and significant correlation between grain yield and height, ear length and grain in the row, which was a significant increase compared to stress conditions. The correlation of traits under full stress conditions also showed that the correlation coefficient between cob length trait and positive height was positive and significant. From the study of correlation coefficients between maize traits in non-stress conditions, it can be concluded that the most important components of grain yield are cob length and grain per row. While the correlation coefficients under moisture stress conditions show that the grain trait in the row has a positive and significant correlation with yield, under stress conditions in the cob stage did not show any traits with correlation yield.
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Studying the effects of traits in the genotype of three maize hybrids in Hungary
97-101Views:766In this study, we selected three hybrids (Armagnac, Loupiac, and Sushi) for evaluation of the effect of traits in genotype in Debrecen. In 2017, the total rainfall from May to October was 314 mm in Debrecen, which was 236 mm in the winter period before sowing. The obtained results showed that there was a positive correlation between the weight of the cob maize and the rate of seed/cob, number of rows with number of seeds in column and outer diameter ear with weight of cob and number of rows in grain per ear and the rate of seed / cob; Also, there was a negative correlation between grain weight in ear with seed/cob rate, outer diameter ear and the rate of seed/cob and outer diameter ear with the number of leaves. There was a positive correlation between stem diameter, Seed/cob rate and the number of nodes by GGE biplot. In addition, there are traits of weight of all seeds and outer ear diameter that had the highest effect on average yield. Moreover, the number of seeds per row showed the least effect on the average yield of hybrids.
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The impact of various grape stock cultivars on the As, Cu, Co and Zn content of the grape berry (must, seed)
39-44Views:475Scientific research from the last decades showed that the inappropriate industrial and agricultural production caused an abnormal increase of the potentially toxic elements in the soil. Unfortunately the acidification of the soil is an increasing problem in Hungary. According to Várallyay et al. (2008) 13% of the Hungarian soils are highly acid. Accumulation of toxic elements differs in the genetically diverse plant species. The root of the plant constitutes a filter so that the rootstock is also kind of a filter system, which may prevent that the scion part (such as berry) accumulate high levels of various potentially toxic elements from the soil. The aim of research was to determine how different grape rootstocks influence the As, Co, Cu and Zn content of the musts and seeds. Thus, specifying which of the grape rootstocks takes up the lowest level of these 4 elements (As, Co, Cu and Zn), and accumulates in berries, so could reduce the potentially toxic element load of the grape berries. The grape rootstock collection of the University of Debrecen was set up in 2003 in 3x1 m spacing on immune sandy soil. Grafting of ‘Cserszegi fűszeres’ was started in 2010. We could evaluate yields harvested from 12 rootstock varieties of the experiment in October 2011. We obtained valuable differences in the arsenic, copper, cobalt and zinc concentrations of musts and seeds of ‘Cserszegi fűszeres’ grafted into different rootstocks. The results obtained from the 2011 harvest support the statement that the choice of rootstock might be an important factor to increase food safety. The differences in concentration of the four elements observed in case of the rootstock may have been caused on one hand by the rootstock effect, and on the other hand, the vintage effect has a very significant impact on the vines element uptake. Several years of experimental results will be needed to answer these questions.
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Behavior of some Hungarian wheat varieties to seed soaking in gibberellic acid under salt stress
7-17Views:349Various abiotic stresses including high salinity strongly affect seed germination. Three Hungarian wheat landraces Gamási, Fóti and Kartali have been tested for seed germination, seedling growth and chemicals parameters when seeds were soaked in gibberellic acid (GA3) at the concentrations of 0, 75 and 150 ppm and at four levels of salinity stress 0, 5, 10 and 15 dSm-1. A laboratory experiment took place at Research Institute of Nyíregyháza. Factorial Experiment based on Randomized Complete Block Design (RCBD) in four replications has been conducted. Seed soaking in GA3 before sowing significantly affected germination characteristics, seedling properties and the results of chemical analysis. The highest germination as well as K+ content of seeds were observed at seed soaking into 150 ppm GA3. However, Na+ and proline contents in seedling were decreased. The behavior of the tested Hungarian wheat varieties was genotype-dependent. Increasing salinity stress up to 15 dSm-1 significantly affected germination characteristics, seedlings parameters and chemical analysis. Fóti variety turned out to be the best at 150 ppm GA3 compared to the other two varieties. Moreover, it had the lowest Na+ and proline contents and highest K+ content.
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Effects of different crop years and sowing date on maize yield
93-96Views:276We carried out the tests in the flood meadow soil formed on the alluvial cone of Nagykereki, Sebes-Körös belonging to the Bihar plane small region. The aim of the study was to analyse the effect of the different sowing date of maize on the yield trend based on a comprehensive study conducted for 6 years (2007–2012).
The sowing date of maize hybrids is a factor that significantly influences yield, however, its effect is not significant in each crop year. In the years when the date of sowing has a modifying effect, the reliable yield level can be reached with optimal sowing date management (24 April).
The advantage of early sowing (10 April) proved to be dominant in the year of 2012, the seeds were placed into the still wet soil therefore shooting was more balanced. Maize seeds sown at the time of optimal (24 April) and late (10 May) sowing dates were placed into the already dry soil, which deteriorated germination and the strength of early initial development that had an effect on the yield.
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Correlations study between the selenium content of wheat grass (Triticum aestivum L.) and wheat seeds grown on different soil types
117-121Views:257In the course of the research we determined selenium and dry-matter content of 35 wheat grasses and 35 wheat seeds. The selenium content of the preparation plant samples was measured by spectrofluorimetric determination (ʎexcitation=380 nm, ʎemission=519 nm) of the resulted piaz-selenol complex. It was established that between the selenium content of the wheat grass and wheat seed the correlation coefficient was 0.36 at p=0.05 level which indicates a medium close correlation. Similarly, there was a medium close correlation between selenium content of the wheat grass calculated on dry-matter basis and total selenium content of the wheat, with a correlation coefficient of 0.40 at p=0.02 level.
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Influence of temperature and variety on seed germination of soybean (Glycine max L. Merr) at different germination times
5-12Views:303Seed germination is an important stage in crop development that affects plant performance, crop yield, and quality. Many factors influence seed germination, and one of the most important factors is temperature. The closer the temperature is to the optimum, the faster germination will occur. Temperature affects seed germination in various plants and varies depending on the variety. Therefore, the effect of temperature on seed germination is necessary to investigate, also for soybean varieties. Soybean is one of the world's most valuable oil-seed crops. Generally, proper seedling establishment and germination of soybean seeds are crucial processes in the survival and growth cycle of the crop. Thus, a study was done to investigate the influence of temperature and variety on soybean seed germination at different times after sowing. The experiment was carried out at the Institute of Agronomy, Hungarian University of Agriculture and Life Sciences (MATE), Crop Production Laboratory. Seeds of two soybean varieties were subjected to three different temperatures: 15, 25, and 35 °C, and two soybean varieties, Martina and Johanna were tested. There were four germination times based on the days after sowing: Days 3, 5, 7, and 9. This experiment was set up with a completely randomized block design and four replications. According to the findings of this study, the Martina variety showed a better germination rate as well as higher vigor and viability than the Johanna variety in the first 12 days after sowing. At temperatures of 15 °C and 25 °C, both varieties achieved comparable percentages of viability, but they were more vigorous at 25 °C due to better total seedling length. Thus, the information gained from this study will indirectly determine and confirm the proper temperature for the initial growth of the Martina and Johanna varieties.
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Occurrence of woolly cupgrass (Eriochloa villosa /Thunb./ Kunth) in Hajdú-Bihar county, Hungary
119-123Views:160Woolly cupgrass (Eriochloa villosa /Thunb./ Kunth) is native to East Asia, it spreads in several parts of the World and causes difficulties in plant protection, especially in maize. Difficulties in control of Eriochloa villosa originated from several reasons: seeds continue to germinate later in the season, significant part of seeds emerges from a deep layer of the soil, and the species is less susceptible to some herbicides applied to maize than other annual grass weeds.
The first report on the occurrence of woolly cupgrass in Hungary was published in 2008, and it reported about the appearance of this species near to Gesztely village (Borsod-Abaúj-Zemplén county), however, no information has been added about spread of the weed in Hungary until now.
A significant population was discovered next to Debrecen (Hajdú-Bihar county) in summer, 2011, and then weed associations were examined in maize, sunflower and stubble-fields on several km2 in the area to estimate the Eriochloa villosa infection. The weed species was found on every maize field bordering with a ground cover of 0.5-4%. Woolly cupgrass occurred inside of the 50% of maize fields, and reached a ground cover of 76% in case of most infected area, in addition it was found in sunflower and stubble-fields.
The spread of woolly cupgrass is expected in this area, which requires the consideration of this species in the planning of weed management technologies. -
Exogenous salicylic acid treatments enhance tolerance to salinity of wheat (Triticum aestivum) plantlets
34-38Views:204Salt stress, an abiotic stress, determines modifications of some biochemical indicators, like, antioxidant enzymes, proline (amino acid
accumulate in higher plants under salinity stress) content, and some physiological processes including: plant growth and development. In
this paper we studied the influence of exogenous treatment of wheat seeds, with 0.1 mM salicylic acid (SA) solution, in the plant response to
salt stress. The treatment was applied by presoaking the seeds in the treatment solution for 12 hours before germination. The results showed
that exogenous 0.1 mM SA solution, administrated to the wheat cariopses significantly ameliorated the negative effect of salt stress in first
week of germination in laboratory conditions. -
Economics of site specific crop density in precision sunflower (Helianthus annuus L.) production
91-96Views:371In this research, the crop density of sunflower was examined, which, thanks to the tools available for precision crop production and knowledge of the market environment of sunflower production, best fits the heterogeneous areas of the given production zones and meets the economic requirements. These components together directly influence the effectiveness of sunflower production. In the year of 2021 and 2022, we carried out a site-specific crop density sunflower experiment in two fields with the same soil type, by sowing significantly different amounts of seeds within the given zones. We have established that the sunflower, although a plant with excellent adaptability, reacts sensitively to the place of production and the effect of the year, in zones with heterogeneous productivity, and shows a reaction to sowing with a variable number of seeds per zone, even when examined based on economic aspects.
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Examination of the usability of the MALDI-TOF method in sunflower genetic identity analyses
33-38Views:308Economically, one of our most important crop is the sunflower. Regarding its production it is essential to use top quality seeds. As the conditions of seed certification and the certification parameters are regulated by laws and ordinances, it is highly important to farmers and seed producers to detect seeds of low quality or dubious origins.
Nowadays, the examination of the cultivar homogenity of sunflower is based on a reference method of the International Seed Testing Association Rules International (ISTA), Chapter 8. This standard reference method uses ultrathin poliakrilamid isoelectric focusing gel electrophoresis (IEF-UTL, or simply IEF). With this work we have set out to compare the results of MALDI-TOF to the test results – used as reference results – achieved by this reference method. The aim is to develop a new, quick, cheap and reliable method. In this article I summarized the results of some of the experiments that will provide basis for my further work.
During our previous experiments we have concluded that out of four extracting agents we can get the most protein markers using NaCl acid buffer, 1 propanol buffer. -
The effects of spraying with onion and garlic extracts on the growth of the plant Anethum graveolens L. and its antioxidant effectiveness
19-24Views:243This experiment was conducted during the 2022–2023 agricultural season in a mixed clay soil at one of the fields of the Faculty of Agriculture, Basra University. The aim of the experiment was to investigate the effect of spraying dill plants (Anethum graveolens L) with aqueous extracts of onion and garlic on their growth. The plants were sprayed with garlic aqueous extract at three concentrations (0, 20, 30 g L-1) and onion aqueous extract at three concentrations (0, 15, 25 g L-1), with three applications at a 15-day interval between each spray. The experiment was designed using a Randomized Complete Block Design (RCBD).
The obtained results showed that the plants sprayed with garlic aqueous extract exhibited significant improvements in most characteristics, except for the fruit content of total dissolved carbohydrates and the yield of fruits from volatile oil. Specifically, the plants sprayed with a concentration of 30 g L-1 outperformed the other two concentrations in terms of plant height (cm), number of branches per plant, and 1000-seed weight for each experimental unit. On the other hand, the plants sprayed with a concentration of 20 g L-1 significantly outperformed in terms of floral inflorescence number per plant and seed yield. Additionally, the plants sprayed with a concentration of 30 g L-1 had the highest chlorophyll content and antioxidant effectiveness of dill leaves (%). However, spraying with garlic extract did not have a significant effect on the fruit content of total dissolved carbohydrates and the yield of fruits from volatile oil.
Regarding the plants sprayed with onion extract, they showed overall superiority in most traits, except for the chlorophyll content of the leaves. Specifically, the plants sprayed with a concentration of 15 g L-1 significantly excelled in plant height, while the plants sprayed with a concentration of 25 g L-1 outperformed in terms of floral inflorescence number per plant and seed yield. The plants sprayed with a concentration of 25 g L-1 also had the highest content of total dissolved carbohydrates in fruits and the highest antioxidant effectiveness of dill leaves and seeds (%). However, the plants sprayed with a concentration of 15 g L-1 had the highest yield of volatile oil. The interaction between the two factors (garlic and onion extracts) had a significant effect on all studied traits, except for the chlorophyll content of the leaves.
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Research on the allelopathic effect among the species Tanacetum vulgare and some agricultural crops
105-109Views:229The research presents the results of the allelophatic effect upon the germination and growth of plants, immediately after springing, viewed as the interaction between the species of Tanacetum vulgare sin Chrysanthemum vulgare and three other crops: wheat (Triticum aestivum L.), barley (Hordeum vulgare L.), rape (Brassica napus ssp. oleifera L.). The experiments that were performed consisted in applying treatments with aqueous extracts obtained from the roots, leaves, stems and flowers harvested from T. vulgare plants upon the seeds of the three agricultural crops mentioned before. In all aqueous extracts, the results indicate the presence of some chemical compounds that have inhibiting allelophatic effect. The plants upon which tests were made showed great sensitivity, the results on their germination and growth being significantly negative and highly significantly negative.
The conclusions of this research sustain the idea of setting new research objectives in order to discover the chemical compounds from T. vulgare extracts that have such a stong effect and the possibilities they offer. -
Comparison of the physiological responses of an oil-pumpkin cultivar and hybrid under different nitrogen supply conditions
65-69Views:420Cucurbita pepo var. styriaca is known as a medicinal crop among other cucurbits. The benefits of cucurbits fruits are very important in terms of human health, purification of blood, removal of constipation, digestion and supplying energy. The mutant styrian oil-pumpkin (Cucurbita pepo L. convar. Pepo var. styriaca Greb.) exposes a complete lack of lignification of the seed testa. For this reason, this kind of naked pumpkin seed is more consumable as snack than other seeds. The vegetative growth of plants is crucial for fruit production. Nitrogen is the most imperative element for the proper vegetative growth and development of plants which significantly increases and enhances yield and its quality by playing a vital role in the biochemical and physiological functions of plants.
Field studies were conducted to study the effect of nitrogen nutrition on certain physiological parameters and their differences between the styrian oil pumpkin hybrid and cultivar (Gleisdorfi Classic cultivar and GL Rustical hybrid). The small block experiment was carried out based on a factorial experiment with a completely randomised block design and four replications in the Research Institute of Nyíregyháza during the summer period of 2017 and 2018. Three different concentrations of nitrogen fertiliser was applied at sowing. Photosynthetic pigments (chlorophyll-a, -b and carotenoids) were analysed as one of the basic factor of efficient photosynthesis. The yields of two genotypes were also measured in all treatments as seed-weight. The contents of photosynthetic pigments were higher in the oil-pumpkin hybrid, mainly the carotenoids in 2018. The vegetation period of 2018 was drier than in 2017, the hybrid oil-pupmkin was more efficient in stress tolerance than the cultivar with higher carotenoids pool. The obtained yield was more pronounced in the oil-pumpkin hybrid than the cultivar in all examined years. The hybrid GL Rustical was more sensitive to nitrogen treatment than the cultivar Gleisdorfi Classic.
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Sunflower in Romanian agriculture
153-163Views:320Romania is one of the largest producers of sunflower closely followed by Bulgaria, Hungary, France and Spain. The total sunflower production increased after 1990, due the increase of cultivated surface during the moderate yield average increasing rate. Last year, Romania was ranked first in the European Union (EU) for the area cultivated with sunflower and the total production of sunflower seeds, according to the National Statistics Institute (INS). Romania's position on the map of sunflower producers area is supported by about 1 million hectares sown. In the segment of sunflower productivity, Romania has an unfavourable position, situated in the last places in the classification of the EU's most productive agricultures. In order to increase production per hectare, it is required to perform measures aimed at the elements of sustainable cultivation technology, including the stability of the production level by ensuring phytosanitary protection.
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Sequence stability at SSR, ISSR and mtDNA loci of common millet (Panicum miliaceum) from the middle ages
10-19Views:259Seed remains of medieval millet, recovered from a 15th century layer (King’s Palace, Budapest, Hungary), showed reddish yellow grain color after rehydrating on tissue culture medium that was close to grain color of modern cultivar Omszkoje. aDNA of medieval c. millet was extracted successfully, analyzed and compared to modern common millets by ISSR, SSR, CAPS and mtDNA. Analyses of fragments and sequences revealed
polymorphism at seven ISSR loci (22 alleles) and at the 5S-18S rDNA locus of mtDNA. CAPS analysis of the 5S-18S rDNA fragment revealed no SNPs in the restriction sites of six endonucleases TaqI, BsuRI, HinfI, MboI, AluI and RsaI. Sequence alignments of the restriction fragments RsaI also revealed
consensus sequence in the medieval sample compared to a modern variety. Morphological characterization of twenty common millet (Panicum miliaceum L., 2n=4×=36) cultivars and landraces revealed four distinct clusters which were apparently consistent with the grain colors of black, black and brown, red, yellow, and white. In the comparative AFLP, SSR and mtDNA analysis modern millet cv. ‘Topáz’ was used. AFLP analysis revealed that extensive DNA degradation had occurred in the 4th CENT. ancient millet resulting in only 2 (1.2%) AFLP fragments (98.8% degradation),
compared to the 15th CENT. medieval millet with 158 (40%) fragments (60% degradation) and modern millet cv. ‘Topáz’ with 264 fragments (100%). Eight AFLP fragments were sequenced after reamplification and cloning. Microsatellite (SSR) analysis at the nuclear gln4, sh1, rps28 and rps15 loci of the medieval DNA revealed one SNP (single nucleotide polymorphism) at the 29th position (A to G) of rps28 locus compared to modern millet.
Mitochondrial (mtDNA) fragment (MboI) amplified at the 5S-18S-rDNA locus in the medieval millet showed no molecular changes compared to modern millet. The results underline the significance of survived aDNA extraction and analysis of excavated seeds for comparative analysis and molecular reconstruction of ancient and extinct plant genotypes. An attempted phenotype reconstruction indicated that medieval common millet showed the closest morphological similarity to modern millet cultivar Omszkoje. -
Screening of paprika (Capsicum annuum L.) varieties resistant to NaCl salt stress
105-110Views:359Salinity stress is one of the environmental factors that negatively affect the growth and production of pepper plants. The 100 seeds' weight was measured. The total fresh weight of five seedlings and the growth rate of one seedling of three paprika varieties were also measured under the influence of NaCl at a salinity level threshold of (3 dS m‑1). The proportion of tissue water content in three pepper cultivars was measured according to a mathematical formula at the end of the experiment. In terms of seed weight, the (Carma) cultivar outperformed other types greatly. Except for the superiority of both (Carma, and Bobita F1) over (Fokusz) variety in total fresh seedlings weight under sodium chloride as abiotic stress, there are no significant differences in the total seedlings' dry weight and the rate of seedling growth. The non-drought-resistant type (Bobita F1) loses water the fastest, at 89.61%, compared to drought-tolerant kinds, which lose water at a slower rate (Carma, and Fokusz). The results demonstrate the (Carma) variety's numerical vigor, particularly in the growth rate. More testing is needed to determine the selection of varieties that are resistant to abiotic and biotic stresses.
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Molecular Biological Approach of Crocus sativus L. and its Allies
112-115Views:481The hay saffron (Crocus sativus L.) is a sterile triploid plant, known in human culture only, with no fertile seeds produced. The origin of saffron is still a mist, however it is assumed to be an autopoliploid mutant or a hybrid. The recent classification and most of the former taxonomic publications define C. sativus to be derived from C. cartwrightianus, a wild species. Because of the sterility of hay saffron it seemed to be reasonable to apply molecular biological methods to complete classical taxonomic studies in examining its relations. The DNA polymorphism based AFLP method has confirmed the close relationship between these species.
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Baobab (Adansonia digitata L.) as a remedy to human livelihood challenges: a review
5-11Views:373Baobab (Adansonia digitata L.) is a unique plant predominant in different ecosystems in Africa. This review assessed its value in relation to human nutrition, health, and related aspects. Our synthesis shows that baobab leaves, seed oil, and fruit pulp were utilised in different forms. Baobab tree products such as fruit pulp, leaves, and seed oil contain high levels of macronutrients, micronutrients, vitamins, carbohydrates, and proteins. Therefore, promoting the utilization and processing of baobab products for improved human nutrition is key. However, the determination of optimal parameters through elemental traceability as well as the relationship between geographical locations and nutrient composition of the baobab plant is required. Altogether, promoting innovative utilization of baobab products by exploring processing techniques to develop novel food and product formulation from Adansonia digitata tree products sheds a green light to sustain human livelihoods.