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Investigation of Potato (Solanum tuberosum L.) Salt Tolerance and Callus Induction in vitro
Published March 4, 2005

Potato production plays an important role in Hungary and the other countries of Europe. Consumption of potato products has increased to a large extent during the past several years. We can satisfy market demands with high quality and virus-free varieties.
Results of potato production depend on tolerance/resistance to abiotic stresses. In man...y cases, increased concentration of NaCl causes yield loss. Selection of salt tolerant varieties proved to be a difficult problem. Nowadays, the salt tolerance of potato varieties can be determined by cell/tissue/ protoplast techniques. Somaclonal variation provides a great potential for selection of lines resistant to salt stress. In vitro shoots and callus, derived plantlets selected for salt tolerance/resistance provide material for micropropagation.
In vitro shoot development of potato (Solanum tuberosum L. cv. Kuroda) was investigated under salt stress (40 mM, 80 mM, 120 mM NaCl) conditions. Shoot heights of plantlets cultured under salt conditions were lower than the control through the investigation. However, the shoot development of plantlets originated from in vitro meristems was almost at the same level as the control under 40 mM NaCl concentration.
There was no significant difference in the in vitro biomass production between control and treatment with 40 mM NaCl concentration. We measured a significant decrease in dry-matter mass under 120 mM NaCl concentration. There is a need for more investigation of different genotypes and for a conclusion as to whether in vitro tolerance could occur under in vivo circumstances in plants originated from somaclones as well.
Under in vitro conditions, we investigated shoot and leaf callus initiation using different culture media with different 2,4-D concentrations. Under dark conditions, callus induction of shoot/leaf decreased as the 2,4-D concentrations increased.
In light conditions, there was a little callus induction, while callus initiation from the shoot from 5 μM to 12 μM 2,4-D concentration showed a significant increase

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Behavior of some Hungarian wheat varieties to seed soaking in gibberellic acid under salt stress
Published May 16, 2017

Various abiotic stresses including high salinity strongly affect seed germination. Three Hungarian wheat landraces Gamási, Fóti and Kartali have been tested for seed germination, seedling growth and chemicals parameters when seeds were soaked in gibberellic acid (GA3) at the concentrations of 0, 75 and 150 ppm and at four levels of salinity s...tress 0, 5, 10 and 15 dSm-1. A laboratory experiment took place at Research Institute of Nyíregyháza. Factorial Experiment based on Randomized Complete Block Design (RCBD) in four replications has been conducted. Seed soaking in GA3 before sowing significantly affected germination characteristics, seedling properties and the results of chemical analysis. The highest germination as well as K+ content of seeds were observed at seed soaking into 150 ppm GA3. However, Na+ and proline contents in seedling were decreased. The behavior of the tested Hungarian wheat varieties was genotype-dependent. Increasing salinity stress up to 15 dSm-1 significantly affected germination characteristics, seedlings parameters and chemical analysis. Fóti variety turned out to be the best at 150 ppm GA3 compared to the other two varieties. Moreover, it had the lowest Na+ and proline contents and highest K+ content.

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Exogenous salicylic acid treatments enhance tolerance to salinity of wheat (Triticum aestivum) plantlets
Published October 5, 2010

Salt stress, an abiotic stress, determines modifications of some biochemical indicators, like, antioxidant enzymes, proline (amino acid
accumulate in higher plants under salinity stress) content, and some physiological processes including: plant growth and development. In
this paper we studied the influence of exogenous treatment of wheat... seeds, with 0.1 mM salicylic acid (SA) solution, in the plant response to
salt stress. The treatment was applied by presoaking the seeds in the treatment solution for 12 hours before germination. The results showed
that exogenous 0.1 mM SA solution, administrated to the wheat cariopses significantly ameliorated the negative effect of salt stress in first
week of germination in laboratory conditions.

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