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Impact of lameness on the milk production of ewes
5-7Views:114The aim of this study was assessing the impact of lameness on the milk production, somatic cells count and component of milk. We assess also impact of lameness on the order of entry into the milking parlour.
The experiment was carried at the farm, located in northern Slovakia. The farm keeps sheep Improved Valachian. Samples of milk were taken during two periods: May, July. It was taken 428 samples together. We recorded three groups by lameness- strong lame, slightly lame, non-lame ewes. We recorded also the order of entry of ewes into the milking parlour in milking row. The results were mathematically processed using the Microsoft Excel program and statistically evaluated by SAS.
We found significant statistical differences between months (P<0.0001) in all the above mentioned indicators. In July we recorded 26 ewes with slightly lameness and 18 ewes with strong lameness. Other ewes were non-lame. Non-lame sheep had in July the highest milk yield (356±148 ml) and the lowest decrease in milk yield from May to July (-206±131 ml) compared with slightly (317±116 ml, -223±163) and strong (319±122 ml, -219±151 ml) lame ewes. However, these differences were not statistically significant. We have not identified statistically significant differences between groups in somatic cells count (logxSCC for non-lame: 4.83±0.608 in ml, slightly lame: 4.76±0.653 in ml, strong lame 4.71±0.787 in ml). Milk composition (fat, proteins, lactose) nor changes in the composition of milk that occurred between May and July were not affected by lameness of ewes. But lameness in July affected the change the order of entry of ewes in the milking parlour in July compared with the order of entry recorded in May.
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Identification of the Slovak traditional cheese “Parenica” microflora
227-239Views:232Numerous studies have demonstrated the higher accuracy, faster time-to-results and lower costs provided by MALDI Biotyper systems compared to classical methods. In this study, the culturable population of total count of bacteria, enterococci, coliforms bacteria, lactic acid bacteria (LAB) and microscopic fungi and yeasts from cow’s dairy products was identified using the MALDI-TOF MS Biotyper. Altogether, 50 samples of the Slovak cheese “Parenica” were examined. Total numbers of bacteria were cultured on Plate count agar at 37 °C for 24–48 h, aerobically; enterococci were cultured on Enterococcus selective agar at 37 °C for 24–48 h, aerobically; coliforms bacteria were cultured on Violet Red Bile lactose agar at 37 °C for 24–48 h, aerobically. The LAB were cultured on MRS (Main Rogosa agar), MSE and APT agar at 30 °C in microaerophilic conditions. The microscopic fungi and yeasts were cultured on Malt extract agar at 25 °C for 5 days, aerobically. Isolated strains (total 669) were subjected to identification by the MALDI-TOF MS. Among total count the identified bacteria mostly were Acinetobacter baumannii, Bacillus cereus, Micrococcus luteus and Staphylococcus warneri. Escherichia coli and Enterobacter cloacae were the most abundant coliform bacteria representatives identified. Coliform bacteria included Citrobacter, Hafnia and Klebsiella. Altogether three genera belonged to the LAB – Lactobacillus, Lactococcus and Leuconostoc were identified with Lactococcus lactis, Lactobacillus plantarum, Lactobacillus coryniformis, L. fructivorans and Leuconostoc mesenteroides were considered as the dominated LAB species in dairy products. Among yeasts, Kluyveromyces lactis, Candida zeylanoides and Yarrowia lipolytica were among the most isolated.
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Fishes protected of Natura 2000 system in Slovakia
9-12Views:69The timely and effective establishment of Natura 2000 network is one of the greatest environmental challenges for the new Member States of the European Union. Slovak Republic is facing this challenge now. By the June 2005, the Government in our country must prepare a list of sites to be included in the Natura network, according to the criteria set up in the Habitat Directive. Natura 2000 sites, according to the specificEUDirectivesconsistofSpecialAreasofConservation(SACs),andSpecialProtectionAreas(SPAs).TheSPAsandtheSACs, the latter selected as Sites of Community Importance (SCIs), will be incorporated into the Natura 2000 network. The problems of proclaiming the system of protected areas of European importance for the Natura 2000 have been one of the major topics of the ichthyological activities in our country in recent years.
For 3 lampreys and 20 fishspeciestheterritoryofpotentialsiteofcommunityinterest(pSCI)hasbeenlimitedandproposed.Theselection of localities and examinations of population state in individual species of fishespresentedintheAnnex(II.,IVandV.)totheCouncilDirective No. 92/43/EEC were carried out in 2003/2004. In total 179 pSCI localities for fishandlampreysinthealpinebiogeographicregion (83) and in the pannonian biogeographic region (96) pSCI in the Slovak republic were selected and limited. The number of pSCI localities selected for individual species is different regards to their distribution within the river net of Slovakia. For example, for species of general distribution, 30 localities pSCI (Misgurnus fossilis, Rhodeus sericeus, Gobio albipinnatus, Barbus barbus) or 35 localities pSCI (Cobitis elongatoides, Barbus peloponnesius), have been proposed, on the contrary, in several species of exclusive distribution as Lampetra planeri - 8, Gobio uranoscopus - 9 and Zingel zingel -12 localities.
The pSCI territory for individual species of the ichthyofauna presented here represents the firststageintheprocessofcreatingtheprotected territory system of European interest Natura 2000 in the territory of the Slovakia for this group of aquatic vertebrates. The list of proposal sites will have to be submitted to the European Commission by the date of Accession. -
Effect of G2548A polymorphism in the leptin gene on the BMI level in human population
5-10Views:129The polymorphism in leptin (LEP 2548A) seems to influence obesity among others genes. The aim of this study is to investigate the effect of the G2548A polymorphism on body mass index. We included 79 people from Slovakia with some genetic relatedness and used barrels kit to isolate the genomic DNA from an adenoblast swab- from the salivary. PCR products were amplified by pursued polymorphisms and G2548A, we restriction-analyzed them and then we identified the specific fragments describing the presence of chosen SNP polymorphism by the agarose electrophoresis, to analyze SNP polymorphism by PCR-RFLP method.
The LEP gene had increased frequency of G allele (0.5506). The most common genotype occurring in the gene LEP was heterozygous genotype (AG) and the least frequent genotype in LEP was AA (0.1899). Taking the age into account the BMI is higher if the G allele occurs in the LEP gene. Moreover, if the G allele genotype was situated in dominant form, then the highest average BMI was present.
According to the results we can assume that the AA genotype (LEP) has a protective effect on the prevalence of obesity compared to the other genotypes.
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The influence of fertilization and tillage method on the formation root system capacity and grain production of spring barley
89-93Views:124To evaluate the interaction of year x variety, year x tillage method and year x fertilization on the grain yield and root system capacity (RSC) of spring barley, we ran polyfactorial field trials in agroecological conditions of a warm corn production area in Slovakia, at Malanta, in 2009 and 2010. The RSC measurements were done using LCR - meter at a frequency of 1 kHz and they took place in four growth stages: at leaf development in the stage of four leaves (RSC1), in full tillering (RSC2), in the stage heading (RSC 3) and at the stage of ripening (RSC4). The values of grain yield, RSC1, RSC2, RSC3, RSC4 reached in 2009 comparison to 2010 were significantly lower. The highest yield in 2009 was reached by variety Marthe (4.49 t.ha-1) and by variety Bojos (7.19 t ha-1) in 2010. The highest values of RSC in observed growth stages were achieved by variety Bojos in 2009, and in 2010 also besides RSC1. Within both years, difference in yields between tillage methods was not observed. The values of RSC in growth stage of 4 leaves and tillering was higher at conventional tillage, butthe values of RSC3 and RSC4 were higher with minimized tillage. The highest grain yield and values of RSC in every growth stage were achieved on the fertilization variant “c“ in 2009 and on the fertilization variant “b“ in 2010. The correlation relationships between grain yield and RSC were significant and positive in every growth stage. The strongest relationship was found among grain yield and RSC (r=0.6047).
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Consumption of fertilizers in districts of Slovakia in the period 2006–2015
389-398Views:153Consumption of fertilizers in districts of Slovakia in the period 2006–2015 Water quality in the surface streams is influenced by several factors. One of important information which can help us to solve problems with quality of water in water body is a distribution of point and non-point pollution sources in a river basin and also amount of pollutants released from them to surface streams. An example of a point source of pollution is the outlet from wastewater treatment plants (industry, urban areas, farms, etc.). On the other hand the most significant non-point source of pollution is considered the application of fertilizers in agriculture.
In this paper we have evaluated consumption of organic and industrial fertilizers in Slovakia in the period 2006–2015. Total (in tonnes) and average (in kg ha-1) consumption of industrial and organic fertilizers was analyzed. In monitored period, the amount of applied organic fertilizers was much higher than the amount of applied industrial fertilizers and in addition a significant part of total fertilizers consumption had nitrogenous fertilizers in a group of industrial fertilizers. In a group of industrial fertilizers during the period 2006–2015 we observed just moderately increasing in their consumption, while in the period 2010–2015 the average amount of applied industrial fertilizers per hectare of agricultural land increased by about 20 kg ha-1. On the other hand, in a group of organic fertilizers we observed a decreasing in consumption of fertilizers.
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Water infiltration into the soil – what do measurements indicate?
343-351Views:141Physical properties of top-soil organic materials significantly influence initiation processes of infiltration and runoff generation. This paper deals with the specifics of water infiltration through the top surface organic layer of the forest soil. Three field methods (Guelph permeameter, Tension disk permeameter, Single-ring method) and one laboratory method (Falling head) of hydraulic conductivity (KS) determination are compared and interpreted in the context of their applicability and limitations. The Falling head method provides far different values of KS if sample cylinders are or are not sealed with grease against the wall effect. The Guelf permeameter is very significant to the position of different horizons’ interface, while Tension disc permeameter results are dependent on antecedent soil moisture. The single ring method is applicable with acceptable results only when there is no abrupt interface between horizons in the vicinity of the ring bottom edge.
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Studies of Cryphonectria parasitica (MURR.) BARR subpopulations on Quercus petraea in Ukraine
70-75Views:95Chryphonectria parasitica (Murr.) Barr, the casual agent of chestnut blight disease, which is one of the most important fungal pathogens of chestnut (Castanea sativa). The disease seriously affected the chestnut in Northern-America and in Europe as well. It is important to mention that the pathogen does not only infect the chestnut but oak species (Quercus spp.) also. In the Carpathian-Basin, the chestnut is endemic in the Mecsek mountains, in Zala, in Somogy counties but it also can be found in the Danube-Bend. In the Carpathian-Basin (outside Hungary) the chestnut is found in Slovakia, Ukraine, Romania. In our study bark samples infected by Cryphonectria parasitica were collected from Bobovyshche, Serednje and Rostovjatica (Ukraine). The rate of infected chestnut tree were higher than 90% around Bobovyshche and beside chestnut, the symptoms were detected on oak trees as well. We collected bark samples from chestnut and oak as well and then we isolated the pathogen Cryphonectria parasitica in the lab of University of Debrecen. Symptomatological observations, laboratory examinations on fungus morphology, as well as comparisons of ITS sequency homology were made and approved that the causal agent of new disease was Cryphonectria parasitica. Our results proved that the Cryphonectria. parasitica infects oak trees beside chestnut in the Carpathian-Basin. Further studies are needed to determine the VCG (Vegatative Compatibility Group) group of the Cryphonectria parasitica found on oak trees.
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Comparative study of regional airports for summing up the competitiveness of Debrecen Airport
103-106Views:100The Debrecen International Airport in commercial means might be the eastern gate of the European Union. This statement is in question these days .In the neighbouring countries like Slovakia, and Romania the old commercial airports have came through major renovation, and technical improvement. With Romania joining to the Schengen Agreement, Debrecen is going to lose its comparative advantage. So, it is time to follow up what has remained from the five years ago still existing technical and commercial priority of Debrecen aerodrome. In this study looking through the technical background of Kosice, Arad, Cluj-Napoca, Targu-Mures, Oradea, Baia-Mare, Satu-Mare, and Debrecen international airports we have found that Satu-Mare, Arad, and Targu-Mures might be major competitors af Debrecen airport. The level of installed technology has exceeded the one in Debrecen in case of Targu-Mures, and Arad. The situation is the same in Kosice which the absolute market leader in the region.
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Investigation of the mycelial compatibility of Macrophomina phaseolina in the Carpatian Basin
25-28Views:100Macrophomina phaseolina is a globally widespread fungal pathogen. The fungus has a very wide range of hosts. Under optimal conditions, M. phaseolina can cause serious damage tothe host plants. In this study, the mycelial compatibility of different M. phaseolina isolates was investigated. From 2019 to 2021, 12 sunflower samples were collected from different regions of the Carpathian Basin, 9 samples from Hungary, 1 sample from Austria, and 2 samples from Slovakia. The genetic variability of the pathogen is a critical problem in plant protection. Two compatible pathogen strains can easily exchange their genetic material for each other, which can lead to the development of resistance. All collected samples (12) were tested to examine their compatibility. Isolates from all tested samples were paired with isolates from all other samples thus, a total of 66 pairings were made. During the examinations of mycelial compatibility, only 20 pairs of all possible pairings were found to be incompatible, and all others (46) were found to be compatible.
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Settlement network, demographic circumstances, healthcare, social service and educations in Szabolcs-Szatmár-Bereg county
105-113Views:154Szabolcs-Szatmár-Bereg county shares its borders with three countries: Romania, Ukraine and Slovakia. The county is part of the North Great Plain Region, it is the third largest county of Hungary in terms of its population. The peripheric geographical location of the county gained importance by the EU accession, as the county represents a significant part of the eastern border of the EU.
Szabolcs-Szatmár-Bereg county is one of the counties of the Great Plain with significant population in the outlying areas, out of the six counties of the Great Plain the population of outlying areas is the lowest in the county, its ratio has been significantly reducing in the past decades. In the six counties in 1990 the population of the outlying areas represented 22.3% of the total population, whereas in Szabolcs-Szatmár-Bereg county the ratio was 13.6%. The ratio has dropped to 6.2% and 4.1% respectively.
The major aim of economic development is the improvement of the living conditions of the population, first and foremost by increasing family incomes. There are two main determining factors of family income level: employment ratio and the sectoral structure of employment.
Between 1992–2010 the number of employees in the financial sector decreased from 89 000 to 75 000, whereas the number of employees in the non-financial sector grew dynamically from 40 000 to 65 000. Structural change was significant. In the non-financial sector the proportion of the two main employment fields (education and healthcare) swapped. The number of employees in the education sector decreased from 16 000 to 14 000, while the number of employees in the health and social care sector grew from 11 000 to 22 000.
On the basis of statistics the number of kindergarten and primary school children reduced in the given period, while the number of secondary school aged children increased. In 1999 the number of secondary school children was 1/5th compared to the number of primary school children, whereas in 2010 their number almost reached 1/2. The student number in higher education doubled, which is a favorable phenomenon, though its structural changes raise some problems. The lack of technical trainings hinders exigent industrial development.
Since 1990 the education level of the population has significantly improved, the number and proportion of secondary school students and university graduates have risen much more dynamically than in other regions of the country. In the county the proportion of secondary school students increased 63% more than the national average, while in the case of university graduates the county passed the average national growth by more than 54%. Due to the effect of structural changes the proportion of university graduates in the corresponding age group improved, its backwardness compared to the national average has ameliorated from 34% to 29%, in the group of secondary school graduates the disadvantage has moderated from 26% to 21%. The computer and internet supply, as well as the number of teachers and students enrolled in secondary education institutions have developed dynamically in the county. Their growth has much excelled the national average.
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Greenhouse gas emissions and Europe 2020 strategy
241-244Views:58Common Agricultural Policy has identified three priority areas for action to protect and enhance rural heritage: (i) the preservation and development of natural farming and traditional agricultural landscapes; (ii) water management and sustainable use and (iii) dealing with climate change. Measures of Rural Development Plan in EU countries promote the development of agricultural practices for preserving the environment and safeguarding the countryside. This is achieved by targeting rural development and promoting environmental friendly, sustainable practices, like agri-environment schemes. Farmers are encouraged to continue playing a positive role in the maintenance of the countryside and the environment. Changes in total emission between 1990
and 2007 do not show any correlation with the total GHG emission. GHG emission was reduced in Hungary, Slovakia, Lithuania, Czech Republic, Romania, Poland, Estonia and Bulgaria, where GHG efficiency is low. -
Fungi detected in trunk of stone fruits in the Czech Republic
121-127Views:206This study was focused on detection of the spectrum of fungi in the wood of stone fruits using molecular genetic methods. Samples were obtained from apricots, plums and sweet cherry trees from region of Moravia, one sample was obtained from Myjava (Slovakia). Segments of symptomatic wood were obtained from dying stone fruit trees with very significant symptoms. This study describes detection of the fungi in the wood of 11 trees in general in 5 localities. The cultivation of the fungi from symptomatic wood and sequencing of ITS was carried out. Eleven fungal genera were determined in the stone fruits wood, particularly Irpex lacteus, Fomes fomentarius, Neofabraea corticola, Calosphaeria pulchella, Cytospora leucostoma, Phellinus tuberculosus, Stereum hirsutum, Collophora sp., Pithomyces chartarum, Aureobasidium pullulans,Fusarium sp. The results of this study demonstrate that the reason of declining of stone fruit trees in Moravia is caused probably by trunk pathogens.
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Integrated development strategy of the catchment area of the Tisza river
31-36Views:181The main challenge for mankind has always been to eliminate the borders of its living space, as well as to explore and discover its new faces. Contemporary literature strengthens this postulate. It is the preference of the experts of various fields in spatial analysis is to consider space to be repairable, changeable and organisable. In accordance with this axiom, the five affected countries in the catchment area of the Tisza river (Hungary, Romania, Ukraine, Slovakia and Serbia) worked out an integrated, mutual standpoint to support the problems of the catchment area and to exploit its opportunities with the aim to support transnational cooperation. They laid great emphasis on the existing resources which could become the driving force behind regional development directions. This study contains the summarised outcomes of the TICAD project (SEE/A 638/4.2./X) which was drawn up as a result of cooperation between renowned institutions of the five affected countries within the South East Europe Transnational Cooperation Programme (lead partner: VÁTI, Hungary).
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The character of the Bódva by the composition of the fishassociations
37-44Views:77The Bódva is one of our rivers that flow outside the Hungarian borders and arrive to the country across the frontier.The Bódva is approximately 110 kilometres long, its upper course is mountaneous- like in its springfield in Slovakia and it reaches the Sajó by flowing among the lower hilly region. The river wall is ravine-like in more places, the river itself is devious, and the water basin is rather variable as in the underlay muddy segments and heavily shingly beds can also be found.
By its fish fauna and physico-chemical characteristics, the Bódva is such aliving water that-after Borne and others (Nowicki, Thienemann) river zone system- can be regarded as an almost uniform typical Barbel zone.
According to our research program (2001-2005) and literature resources we estimated the fish fauna of the Bódva on both the Slovakian and Hungarian course. From some four decades to the wide ecofaunistic examinations of the near past we surveyed the quondam and present fish species of the Bódva River. According to our survey some 40 species compose the recent fish fauna of this river.
In the fish fauna of the Bódva those fish species that presently own a tight ecospectrum, sensitive to the changes of the environment thus so called indicator species, can also be found just like the less sensitive, invasive, aggressively reproducing species. By examining the changes in the fishfauna, the complex of those biogen and abiogen processes that affect the river can be indicated well. It can be stated that the fishfauna of the Bódva is rather rich with a countrywide measure also, and maintain a lot of rare, regressive protected and worth while protection species.
In the last period of the construction, the racial and quantitive composition of the fishfauna of the upper course of the Bódva has changed significantly. Certain species (in the Hungarian course) has vanished almost totally or at least their number declined not ably. Among the vanishing species there are not only a few substantial, protected species of the fauna (e.g. Barbus pelononnesius petényi, Leucaspius delineatus, Zingel sterber, Barbatula barbatula), but the number of the once mass species also decreased appreciably (e.g. Rhodeus sericeus, Alburnoides bipunctatus, Cobitis elongatoides). With the above mentioned changes the number of other animals also decreased in the course that we examined (e.g. mussels, crabs). We examine the cause of these changes in the pollution of the river and according to that in the decrease of the food source and the negative changes of the physico-chemical parameters of the water.