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• Search for polymorphism in exon 5 of cattle pituitary adenylate cyclase activating polypeptide gene

  17-20

  Ádám Csikós

  Ádám Simon

  Krisztina Homonai

  Gabriella Gulyás

  Ákos Tisza

  Andrea Tamás

  Dóra Reglődi

  András Jávor

  Levente Czeglédi

  Views:

  201

  Pituitary adenylate cyclase-activating polypeptide is a neuropeptide expressed primarily in the hypothalamus and in other tissues, which divergent and extensive physiological functions are proved. Large number of SNPs in PACAP are published involved SNP that is associated with phenotypic trait of cattle as well. Hungarian Grey, Hungarian Simmental, Holstein, Charolais and Angus cattle breeds were involved in this study to search for polymorphism in exon 5 1–391 bp and G/A transition. Our results of PCR RFLP and PCR SSCP did not prove the occurrence neither G/A transition, nor any other SNP in cattle breeds involved.

• Tenability of ram semen

  63-66

  János Oláh

  Tamás Pécsi

  András Kovács

  Anna Pécsi

  András Jávor

  Views:

  200

  It could be stated that the diluted semen of Awassi rams taken in the breeding season preserved its fertilizing abilityat different temperatures for different periods of time. The motility of spermatozoa kept at 23 vs. 8^oC was checked daily. The largest spread of data was observed 24 hours after taking the semen, then the motility rate of cells showed a linear decrease. Motility results of fresh and frozen-thawed samples were compared also after heat resistance test and significant differences were found between these groups. Significant individual differences were observedin the sperm motility after heat resistance test.