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Application of yeasts fortified with microelements – Review
101-106Views:233Microelements are increasingly becoming into the focus of interest from both a point of view of nutrition science and feeding. An always growing care must be paid to the microelements coverage both in human and animal organisms because of incorrect alimentation habits and
unsatisfactory feedstuff nutrition value. For the increased supply of the micro-nutrients, enrichment or fortification with microelements can not only be realized with traditional foodstuffs and forage but there are already alternative ways such as single-cell proteins from yeasts directly enriched or fortified with microelements for the purpose.
We would like to draw the attention that the production of these items is more favourable in comparison with traditional foodstuffs or forage since yeasts are capable to multiply microelement levels compared to their original state, and establish organic bonds with them.
For this purpose, we explored and analysed the scientific literature, studies and research results on this subject, that is why we stressed the significance of yeasts, the features and health effects of certain microelements, as well as the possibilities for use of yeasts enriched with micro-nutrients. -
Preliminary study: The effect of gibberellic acid and girdling application on two dual-purpose grape varieties in a cool climate
139-144Views:474In our 2023 experiment, Nero and Muscat Pölöskei grapevines were treated with gibberellic acid (12 mg L-1) during flowering, followed by stem-end girdling at the onset of berry development. Photosynthetic activity was monitored regularly. Assimilation rate (A, µmol CO₂ m⁻² s⁻¹) and transpiration rate (E, mol H₂O m⁻² s⁻¹) were measured under natural light. Sugar content and acidity were assessed via refractometry, while berry dimensions and texture (e.g., berry hardness [Bh, mN]) were recorded. Gibberellin treatment increased sugar content compared to both control and girdled samples. However, girdling significantly enhanced sugar accumulation in Muscat Pölöskei compared to untreated samples. Gibberellin also induced berry elongation, increasing the longitudinal axis in Nero (19%) and Muscat Pölöskei (24%). Girdling temporarily reduced photosynthetic activity, which recovered post-healing. The elongated berries due to gibberellin treatment led to a looser cluster structure, which is beneficial for Nero, which is prone to fungal diseases due to dense clusters.
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Evaluation of reduced tillage technologies in corn production based on soil and crop analyses
47-54Views:511Despite new cultivation methods, the proportion of conventionally cultivated land is still very high in Hungary.
Although these technologies demand more time, labour and fuel, they are still attractive to users because they require less professional skill and simple machinery. In Hungary, conventional tillage methods usually lead to soil deterioration, soil compaction and a decrease in organic content. These side effects have caused gradually strengthening economic and environmental problems.
The technologies for those plants which are dominant on Hungarian arable lands use (winter wheat, maize, sunflower and barley) need to be improved both in the interest of environmental protection and the reduction of cultivation costs.
The Department of Land Use at Debrecen University is cooperating with KITE Sc. to carry out soil tillage experiments at two pilot locations to prove tillage technologies already used in the USA.
The aim of our examination is to adapt new technological developments and machinery, and to improve them on Hungarian soil for local environmental conditions. With these improved machines, the field growing of plants could be executed by less manipulation and better suited to economic and environmental needs. The most significant task is to investigate and improve the conventional cultivation replacing, new soil-protecting tillage technologies, and to apply no-till and mulch tillage systems.
On the basis of the experiments’ survey data, we established that the looseness and moisture content of the soil using reduced tillage is more favourable than after using conventional technologies. The results of no-till and shallow spring tillage are behind those of winter plough or disk ripper cultivation in corn yield and production elements.
To preserve moisture content in the soil, the ground clearing and sowing while simultaneously performing no-till method presents the most favourable results. The surplus moisture gained using no-till technology is equal to 40 mm precipitation.
Regarding the yield of winter wheat we established that the tillage methods do not affect plant yield. Both disk ripper and conventional disc cultivation showed nearly the same harvest results (5.55 or 5.5 t/ha), where the difference is statistically hardly verifiable from the no-till method. From the individual production of corn and the number of plants planted in unit area, calculated results prove that no significant difference can be detected between the production of winter plough and disk ripper technology. Although the yield achieved with the no-till method is less than with the previously mentioned technologies, the difference is only 9-10%. We received the lowest production at shallow spring tillage.
Evaluations have shown a 1.1 t/ha (13%) difference in the yield of maize, between winter tillage and the disk ripper method, in this case the traditional method resulted in higher yield. In winter tillage, the yield of maize was 1.9-2.1 t/ha (23-25%) higher than in the case of direct sowing and cultivator treatments. No significant difference could be noted between the yields of direct sowing and cultivator treatments.
Our research so far has proved the industrial application of reduced tillage methods in crop cultivation technologies. -
Usage of different remote sensing data in land use and vegetation monitoring
7-12Views:297The use of remote sensing in forest management and agriculture is becoming more prominent. The rapid development of technology allowed the emergence of database suitable for precision application in addition to the previously used low-resolution and low data content images. The high resolution, hyperspectral images are not only suitable for separating the different land use categories and vegetation types but also for examining the soil characteristics and biophysical features of plants (Blackburn and Steel, 1999; Condit, 1970). We processed a multispectral satellite image (Landsat 7 ETM+) and a hypespectral areal image (DAIS 7915) about a farm on the plains and evaluated the different image classification methods. During our examinations, we examined the geometrical and radiometrical characteristics of images first, then assigning the training areas, we determined the spectral characteristics of land use categories. We performed a multispectral analysis for checking land use, where we compared controlled and uncontrolled classification systems to check their reliability. We used areal and spectral reductions to make the classifications more accurate and to reduce the length of calculations.
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Examination the effects of different herbicides on the soil microorganisms of a calcareous chernozem
121-126Views:172Pesticides play a key role in fighting weeds, pests and parasitic fungi. According to surveys, pests reduce the yield of agricultural crops by 35% worldwide. Pests, fungi and weeds account for 14%, 12% and 9% yield loss, respectively (Gáborjányi et al., 1995). Chemicals have contributed to increasing and maintaining the yields of crop production for decades. Today, agricultural production (in spite of many efforts) is unthinkable without the use of pesticides (herbicides, insecticides and fungicides). On the other hand, these chemicals contribute to the pollution of the atmosphere, surface and underground waters, and agricultural soils, especially if they are applied improperly.
The sustainable agricultural production pays attention to environment-friendly cultivation-technologies; but at the same time it makes an effort to produce good quality and economical products. The examination of the herbicides’ secondary effects, fits into this chain of idas namely, how the herbicides affect – stimulating or inhibiting – the soil microbiological processes, prevention of soil fertility.
In the course of the experimental work the effect of herbicides on soil biological properties were examined in different maize (Zea mays) cultures. We wanted wished to know that how the herbicides affect the quantity change of soil microorganisms, the life of different physiological groups of bacteria and the activity of microorganisms. A small pot experiment was set up in 2008 with the application of two herbicides - Acenit A 880 EC and Merlin 480 SC – in the breeding house of the Department. The moisture content and nutrient supply were at optimal level in the experiment.
On the basis of results the following can be stated:
1. It can be stated that the two herbicides and all their doses affected negatively the number of total soil bacteria, the
inhibiting effects were significant. The quantity of microscopical fungi increased by the effect of Merlin 480 SC and decreased in the treatments of Acenit A 880 EC.
2. The Acenit A 880 EC had stimulating effect on the nitrate mobilization. The CO2-production was stimulated by the basic doses of herbicides; the other treatments did not influence the CO2-production significantly.
3. The quantity of microbial biomass-carbon –except for only one treatment- decreased significantly by the effect of herbicides. Besides it, the quantity of microbial biomass-nitrogen increased significantly in the treatments of Acenit A 880 EC.
4. The biomass of test plant decreased in the treatments of herbicides, their quantities were smaller than in the control. In the pots treated by Merlin 480 SC, parallel with the increase of doses decreased the quantity of plant-biomass. -
Lessons of a stripe rust epidemic in a wheat fungicide trial in Debrecen
35-46Views:253During the 2014 year a fungicide application trial was made as a part of technology development in Debrecen (East-Hungary). Both in this trial and across in Hungary a serious stripe rust epidemic developed and caused great yield losses. The first sympotoms were observed in April on one of trial plots and during some following weeks a serious infection grown up. On the base of 13 fungicide active ingredients and dosages by two spraying applications, their efficacy could be evaluated in stripe rust control. Picoxystrobin 250 g/L acive ingredient applied in 0.6, 0.8 and 1 L/ha dosages (Acanto), picoxystrobin 200 g/L + ciproconazole 80 g/L combined active ingredients in 0.5, 0.75, and 1 L/ha dosages (Acanto Plus), epoxiconazole 83 g/L, moreover protioconazole 125 g/L + tebuconazole 125 g/L combinations gave good efficacy, respectively. The yield of well protected plots were 5 to 8 t/ha, but the losses were 40-70% when fungicides with no sufficient efficacy in stripe rust control were applied. The great infection, which was observed on different grasses late in the Autumn, 2014 might forecast a further stripe rust epidemic for the 2015 year.
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Development opportunities for storing and displaying of spatial data in long-term experiments
81-86Views:213Long-term experiments are required to evaluate the impact of irrigation, nutrient utilization, and year factor as well as to assess the potential consequences of climate change. However, in the long-term experiment, it may be necessary to display spatial data for each parcel, either for investigation of soil heterogeneity or presentation. This article aims to provide help for researchers working in long-term experiments for storing and displaying spatial data.
After the outlines of each experimental site were measured with GPS, a spatial database has been created in Quantum GIS. Then, a filter script in R statistical environment using RStudio graphical interface was written. The script helps avoid the QGIS data input interface so that large data can be attached to each parcel directly and as a result there is no need for a separate data entry, only the basic statistical database.
The created GIS database can be used in many ways; it can be exported to KML file format that can be displayed using Google Earth. It is possible to view exported KML files in Google Drive with importing them to Google My Maps application, and with that a browser can display the map. With the Google Drive the maps can be shared within the research group, additionally the outlines can be edited and it is possible to upload the measurement data to the attributes table to existing empty table columns. The map created in Quantum GIS can be used for presentation purposes.
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The Target Areas of Regional Development, Tool and Institutional Background, its Present and Future Prospects
138-142Views:211Regional politics determines the aims of regional structures, and the tools with the institutional background on the whole necessary achieve these. Realizing the aims of regional politics is a precondition of executing the basic principles of regional development and politics. The regional institutional system for handling the decentralized financial resources has not evolved in Hungary yet. The professional institutes for preparing the programs have not been set up, and the management of execution is slowly taking shape. There are still deficiencies in the domestic application of concentration and addition principles. A number of funds are provided on a national level, that are directly or indirectly aimed at regional development. The obvious coordination among the funds is not carried out neither on a national, nor on a regional level, partly to avoid parallel consumption aims. The future of Hungarian spatial structure depends on the type of decentralization strategy the country will follow for the new resources after the accession. Total decentralization offers the most efficient solution for Hungary.
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The impact of different fertilization methods on some microbiological soil characteristics
119-126Views:275In our experiment, we studied the impact of an organic fertilizer, Bactofil® A10 (half- and full dosage applied in field practice) and an artificial fertilizer of Ca(NO3)2 content in different dosages (20-40 mg kg-1) – in addition to control treatments – on two different soils (calcareous chernozem, humus sandy soil) in 2005-2006, the experiment was complemented with treatments applying 250% dosage (100 mg kg-1 N, Bactofil® A10 2.5 times the field dosage) and a compost from urban sewage (25 g kg-1 compost) was also tested on these two soil types. In the
experiment, several soil microbial parameters were studied. The experiment was set up at the Department of Agrochemistry and Soil Science using 1-kg pots.
Our laboratory experiments were performed at the soil microbiology laboratory of UD CAS Department of Agrochemistry and Soil Science, the total number of bacteria, microscopic fungi, nitrifying and aerob cellulose-decomposing bacteria were determined together with the CO2-production of soil, N content of the biomass and urease enzyme activity.
Statistical analysis of the data was done using the program SPSS 13.0, means of the measurements, deviation and significance values were calculated.
In 2005-2006, the effect of the different dosages of Bactofil® A10, and the Ca(NO3)2 fertilizer on the examined microbial parameters of calcareous chernozem and humus sandy soils can be summarized as follows:
• Concerning the total number of bacteria, both treatments were effective on calcareous chernozem soil, the higher (significant) increment in bacteria number was observed in the artificial fertilizer treatments, while in the humus sandy soil Bactofil treatments had a beneficial effect. The number of microscopic fungi also increased in both treatments, higher numbers were observed in the average of two years in the Bactofil treatments.
• The number of nitrifying bacteria was 2.5 times higher in both high-dosage treatments on calcareous chernozem soil, while on humus sandy soil a slight (not significant) increment was observed only int he high-dosage Bactofil treatment. The amount of aerob cellulose-decomposing bacteria significantly increased on calcareous chernozem soil in both the highdosage artificial fertilizer and the small-dosage Bactofil treatment, however, on humus sandy soil no significant increase was observed in either treatment.
• The CO2-production increased in both soil types, although it was not significant in either treatment. A higher (though not significant) soil respiration was observed in the Bactofil treatments in both soil types.
• The microbial biomass N values were significantly higher in the high-dosage Bactofil treatments, however, the high-dosage artificial fertilizer treatment also increased these values significantly on calcareous chernozem soil.
• On calcareous chernozem soil, urease activity was significantly increased and reduced by high-dosage artificial fertilizer treatments and Bactofil treatments, respectively. On humus sandy soil, urease activity was also reduced except for the high-dosage artificial fertilizer treatment. In 2007, the pot experiment with 250% dosages was complemented with the application of compost rich in organic matter, the results of these treatments are sumnmarized as follows:
• In the case of the total number of bacteria, all three treatments resulted in a significant increase on calcareous chernozem soil with the highest values in the Bactofil treatment. The Bactofil treatment was the most effective on the humus sandy soil, but the artificial fertilizer treatment also
resulted in a significant increment. In the case of the total number of fungi, Bactofil treatments resulted in the highest values on both soils, but the compost treatment also increased the number of fungi in calcareous chernozem significantly.
• The number of nitrifying bacteria was increased most (significantly) by the Bactofil and compost treatments on both soil types. The amount of cellulose-decomposing bacteria was significantly increased by he compost treatment on calcareous chernozem soil, while its effect was not significant on humus sandy soil. The number of these bacteria was increased significantly by the Bactofil treatment on humus sandy soil.
• On calcareous chernozem soil, all three treatments significantly increased CO2-production, while the compost treatments had the resulted in the largest increment in soil respiration on both soil types.
• The soil biomass N content was significantly increased in both soils by the compost treatment, while in the case of the humus sandy soil, the Bactofil treatment also resulted in a significant increment.
• Urease enzyme activity was significantly increased by the artificial fertilizer treatment on both soils. In calcareous chernozem soil, the Bactofil treatment resulted in a slight (not significant) reduction in enzyme activity. In humus sandy soil, the Bactoful treatment also resulted in a slight reduction, while the compost treatment increased (though not significantly) the urease activity.
Based on our results, it can be stated that all three treatments were effective with respect to the studied soil microbial parameters. For both the calcareous chernozem and the humus sandy soil, the organic fertilizer Bactofil and the compost with high organic matter content had a stronger effect on some soil microbial parameters than the artificial fertilizer. -
The role of the hybrid-specific technological recommendations of maize in precision crop production procedures
297-302Views:205The necessity of application of hybrid specific crop production technology has been confirmed not only by trial results but also by the experiences gained from the agricultural practice. For this reason it is essential to test and collect data in field trials about the specific agronomic traits of the corn hybrids belonging to different maturity groups and genotypes. Corn hybrids are tested for their responses to sowing time, plant density and fertilizer supply; sensitivity to herbicides; and lately, the resistance to the damages caused by the larvae of corn root worm. Last but not least, mention should be made of the differences in the responses of the corn hybrids to the damages caused by drought stress. Based on the trial results, suggestions for the hybrid specific corn production are compiled and made public for the experts and farmers engaged in corn growing. Corn hybrids may deliver maximum yields on the impact of specific crop production technology only in case if it relies on carefully done general production technology including soil cultivation, seed bed preparation and weed control. Similarly, precision crop production technology may advance the yield increase in economic way if it is constantly drawing on the source of research results.
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Effect of different weaning time on the growth and survival ofCommon carp (Cyprinus carpio L.) larvae
55-59Views:747Within our experiment, we studied the combined effect of live food application and feed training on the production parameters of carp larvae. In addition, we examined whether there is a difference in the growth rate and survival rate of the specimens of Szeged mirror landrace reared in similar stocking density but with different treatments.
Specimens of Szeged mirror landrace from induced propagation were placed into 12 pieces of 40-liter aquaria. For the 4 different treatments, (K, A3, A6, A9), live food (Artemia salina) was fed, as well as feed training was applied.
We also sought to find an answer as to how our feeding and rearing conditions can affect the production parameters of common carp fries and what impact these conditions may have on their survival rates. At the end of the 2-week experiment we determined the individual weight gain of the carp fries, their survival rate and the biomass growth. The purpose of the study is to examine the optimum timing of shifting from live food to feed for carp larvae.
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Effect of tillage practices, fertilizer treatments and crop rotation on yield of maize (Zea mays L.) hybrids
43-48Views:410This research was conducted at the University of Debrecen Látókép Research Station and is part of an ongoing long-term polyfactorial experiment. The impact of three tillage systems (Mouldboard plowing-MT, Strip tillage-ST, Ripper tillage-RT) and two levels of fertilizer treatments (N80 kg ha-1, N160 kg ha-1) along with a control (N0 kg ha-1) on the yield of maize hybrids (Armagnac- FAO 490 & Loupiac-FAO 380) cultivated in rotation with winter wheat was evaluated during a two-year period (2017–2018).
Amongst the three tillage treatments evaluated, ripper tillage (RT) had the highest average yield (10.14 t ha-1) followed by mouldboard tillage (MT) and strip tillage (ST) with 9.84 and 9.21 t ha-1 respectively. Yield difference between RT and MT was not significant (P>0.05), as compared to ST (P<0.05). Soil moisture content varied significantly with tillage practices and was highest in ST, followed by RT and MT (ST>RT>MT). Yield of RT was 7–9% higher than MT in monoculture plots, while MT reign superior in biculture plots (monoculture: RT>MT>ST; biculture: MT>RT>ST).
A positive interaction between tillage and fertilization was observed, with higher yield variation (CV=40.70) in the non-fertilized (N0) plots, compared to those which received the N80 (CV=19.50) and N160 kg ha-1 (CV=11.59) treatments.
Incremental yield gain from increase fertilizer dosages was significantly higher in monoculture, compared to biculture. There was no significant difference in yield between N160 and N80 in the biculture plots (12.29 vs 12.02 t ha-1). However, in monoculture plots, N160 yield was 23% higher than the N80 kg ha-1 (N160=11.74 vs N80=9.56 t ha-1).
Mean yield of maize in rotation with winter wheat was 28% (2.47 tons) higher than monoculture maize. The greatest benefit of crop rotation was observed in the control plots (N0) with an incremental yield gain of 4.39 tons ha-1 over monculture maize (9.92 vs 5.43 t ha-1).
Yield increased with higher fertilizer dosages in irrigated plots. Fertilizer application greatly increased the yield of maize and accounted for 48.9% of yield variances. The highest yield (11.92 t ha-1) was obtained with N160 kg ha-1 treatment, followed by N80 kg ha-1 (10.38 t ha-1) and N0 kg ha-1 (6.89 t ha-1) respectively.
Overall mean yield difference between the two hybrids was not statistically significant, however, yield of FAO 380 was 3.9% higher (9.06 vs. 8.72 t ha-1) than FAO 490 in monoculture plots, while in biculture plots, FAO 490 was 4.1% higher than FAO 380.
Average yield in 2018 was 13.6% (1.24 t ha-1) higher than 2017 for the same set of agrotechnical inputs, thus, highlighting the significant effect of cropyear.
Armagnac (FAO 490) cultivated in rotation with winter wheat, under ripper tillage and N80 kg ha-1 is the best combination of treatments for optimum yield.
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Price Risk Management by Futures Markets and Public Warehousing
58-65Views:185The principal achievement of this paper is the combinative use of two market institutions: public warehousing and commodity exchange and how their joint application is beneficial for the players on the grain market. Based on a theoretical foundation, a calculation model was developed in order to assist short and long-term marketing decisions. It allows all the three participants of the market: producers, consumers and traders, to use this model in order to establish their own business strategy. The model can be used to analyse factors influencing the establishment of price; therefore, it can be also used for policy-making decisions.
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Application fields of wood as a renewable energy source in Europe
31-35Views:220Fuelwood, and wood wastes provide almost half of the renewable energy production of the European Union. Enhancing the use of wood in renewable energy production has more constraint than wind- or solar energy. Forests in the EU member states are sustainably used, they are increasing both in terms of area, and growing stocks. There are possibilities to enhance the fallings and use more fuelwood. Short rotation coppices could fulfill the long term demand for fuelwood; because these plantations surpass the dendromass yield of forests.
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Management of phytopathogens by application of green nanobiotechnology: Emerging trends and challenges
15-22Views:529Nanotechnology is highly interdisciplinary and important research area in modern science. The use of nanomaterials offer major advantages due to their unique size, shape and significantly improved physical, chemical, biological and antimicrobial properties. Physicochemical and antimicrobial properties of metal nanoparticles have received much attention of researchers. There are different methods i.e. chemical, physical and biological for synthesis of nanoparticles. Chemical and physical methods have some limitations, and therefore, biological methods are needed to develop environment-friendly synthesis of nanoparticles. Moreover, biological method for the production of nanoparticles is simpler than chemical method as biological agents secrete large amount of enzymes, which reduce metals and can be responsible for the synthesis and capping on nanoparticles.
Biological systems for nanoparticle synthesis include plants, fungi, bacteria, yeasts, and actinomycetes. Many plant species including Opuntia ficus-indica, Azardirachta indica, Lawsonia inermis, Triticum aestivum, Hydrilla verticillata, Citrus medica, Catharanthus roseus, Avena sativa, etc., bacteria, such as Bacillus subtilis, Sulfate-Reducing Bacteria, Pseudomonas stutzeri, Lactobacillus sp., Klebsiella aerogenes, Torulopsis sp., and fungi, like Fusarium spp. Aspergillus spp., Verticillium spp., Saccharomyces cerevisae MKY3, Phoma spp. etc. have been exploited for the synthesis of different nanoparticles. Among all biological systems, fungi have been found to be more efficient system for synthesis of metal nanoparticles as they are easy to grow, produce more biomass and secret many enzymes. We proposed the term myconanotechnology (myco = fungi, nanotechnology = the creation and exploitation of materials in the size range of 1–100 nm). Myconanotechnology is the interface between mycology and nanotechnology, and is an exciting new applied interdisciplinary science that may have considerable potential, partly due to the wide range and diversity of fungi.
Nanotechnology is the promising tool to improve agricultural productivity though delivery of genes and drug molecules to target sites at cellular levels, genetic improvement, and nano-array based gene-technologies for gene expressions in plants and also use of nanoparticles-based gene transfer for breeding of varieties resistant to different pathogens and pests. The nanoparticles like copper (Cu), silver (Ag), titanium (Ti) and chitosan have shown their potential as novel antimicrobials for the management of pathogenic microorganisms affecting agricultural crops. Different experiments confirmed that fungal hyphae and conidial germination of pathogenic fungi are significantly inhibited by copper nanoparticles. The nanotechnologies can be used for the disease detection and also for its management. The progress in development of nano-herbicides, nano-fungicides and nano-pesticides will open up new avenues in the field of management of plant pathogens. The use of different nanoparticles in agriculture will increase productivity of crop. It is the necessity of time to use nanotechnology in agriculture with extensive experimental trials. However, there are challenges particularly the toxicity, which is not a big issue as compared to fungicides and pesticides.
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Examination of Possibilities for Increasing Sugar Content
202-206Views:107The result of sugar beet production is highly effected by the quality and the yield. Those agricultural techniques are very important which can be applied at low cost to improve these parameters. Such an opportunity is to increase sugar yield by foliar fertilization with nutrients and by treatment with bioactive fungicides.
In my small block trial I examined the effect of the magnesium and strobilurin active ingredients in different combinations and at different application times on the quality and the yield of sugar beet.
I found that there was a signifficant increase in sugar content on that blocks which were treated with a bioactive ingredient combined with magnesium in the middle of July and on those which were sprayed with bioactive ingredient in August. -
Selection of powdery mildew resistant and susceptible grapevine genotypes with molecular markers
100-104Views:287Incorporation of competitive quality and resistance against the most important fungal diseases (powdery and downy mildew) in a cultivar is one of the most important aims of grapevine breeding. In the 20th century, the most advanced results in grapevine resistance breeding were achieved by French researchers. They used resistant cultivars in more than 30% of their growing areas. In these varieties, North American wild Vitis
species were the resistance gene sources. The discovery of immunity-like resistance of Muscadinia rotundifolia opened new perspectives in resistance breeding. M. rotundifolia harbours a dominant powdery mildew gene, providing resistance in highquality cultivars after back-crosses with V. vinifera varieties. M. rotundifolia has been involved in the Hungarian grape breeding programs since 1996, thanks to a French-Hungarian variety exchange. In addition to traditional selection methods, application of MAS (Marker Assisted Selection) based on various types of
molecular markers, can provide additional tools for these efforts. Run1 locus, responsible for powdery mildew resistance, was identified in Muscadinia rotundifolia. Molecular markers closely linked to this locus are very significant in screening progenies deriving from M. rotundifolia and V. vinifera crosses, making possible the discrimination between resistant and susceptible genotypes at DNA level. In our analyses BC5 progeny of {(M. rotundifola×V. vinifera) BC4}×Cardinal (V. vinifera) tested for powdery symptoms were analysed with PCR-RFLP (GLP1- 12P1P3) and microsatellite markers (VMC4f3.1, VMC8g9). Our results proved the applicability of the linked markers and reliability of marker assisted selection. -
The effect of NPK fertilization and the plant density on maize yield and bioethanol production
13-18Views:266For industrial (bioethanol) production of maize, a new production technology is needed. I tested and selected hybrids appropriate for this purpose and set up fertilization and plant density experiments. The experiment were set up on chernozem soil in 2008.
In bioethanol production, the selection of a high-yielding hybrid with high starch content, a slight reduction of N, increase of potassium, the application of the highest plant densities of the optimum interval, harvest at full maturity (when starch content is the highest compared to protein content) are of great importance. -
Development opportunities of biomass-based ethanol production in relation to starch- and cellulosebased bioethanol production
71-75Views:260The biomass is such a row material that is available in large quantities and it can be utilizied by the biotechnology in the future. Nowadays the technology which can process ligno cellulose and break down into fermentable sugars is being researched. One possible field of use of biomass is the liquid fuel production such as ethanol production. Based on the literary life cycle analysis, I compared the starch-based (first generation) to cellulose-based (second generation) bioethanol production in my study considering into account various environmental factors (land use, raw material production, energy balance). After my examination I came to the conclusion that the use of bioethanol, independent of its production technology, is favorable from environmental point of view but the application of second generation bioethanol has greater environmentally benefits.
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Study of factors controlling the amount of 0.01 M CaCl2 extractable Norg fraction
437-449Views:289The use of new methods describing the “readily available” nutrient content of the soil is spreading on a global scale. The 0.01 M CaCl2 extractant is a dilute salt solution in which the easily soluble inorganic (nitrate-N and ammonium-N) and organic N fractions, P, K and micronutrients are also measurable. The 0.01 M CaCl2 has been tested in the University of Debrecen, Institute of Agricultural Chemistry and Soil Sciences since the 90’s. The results of the researches related to organic N fraction, performed in the last decades, and the results of the present study (originating from the long-term experiment of Karcag, 2007–2009) can be concluded as follows:The measurement of easily soluble and oxidizable organic nitrogen (Norg), besides inorganic fractions, could improve the nutrient management.The amount of the Norg fraction is determined by the soil conditions, therefore it is considered to be a site-specific parameter.Management practices and cropyear affect the amount of Norg as well. The present research confirmed that, the effect of fertilization on the amount of Norg can be explained by the changing of the yield (related to total biomass production), while the effect of cropyear is related to the differences in mineralization circumstances and yield as well.The measurement of the Norg fraction is increases the accuracy of N-supply, therefore it could prevent the environmentally harmful excess N application as well. -
Studies of the influences of different N fertilizers and Microbion UNC bacterial fertilizer on the nutrient content of soil
134-140Views:219A field experiment was conducted to examine the effects of different nitrogen fertilizers in combination with bacterial fertilizer on
nutrient uptake of horseradish and plant available nutrients of the soil. Three different N fertilizers, ammonium-nitrate, urea and calciumnitrate
(116 kg ha-1 N) in combination with Microbion UNC bacterial fertilizer (2 kg ha-1) were applied as treatments in a randomized
complete block design in three replications. In this paper we presented the results of soil measurements. The soil of the experimental area
was chernozem with medium sufficiency level of N and P and poor level of K.
Our main results:
The amount of 0.01M CaCl2 soluble inorganic nitrogen fractions, NO3
--N and NH4
+-N and also the quantity of soluble organic-N were
almost the same in the soil. N fertilizers significantly increased all the soluble N fractions. The amount of NO3
--N increased to the greatest
extent and the increase of organic N was the slightest. We measured the largest CaCl2 soluble NO3
- -N and total-N contents in the plots
treated with ammonium-nitrate, the largest NH4
+-N in the plots treated with calcium-nitrate and the largest organic-N fraction in plots
treated with urea.
Bacterial inoculation also increased both soluble inorganic nitrogen forms and also total-N content of soil compared to the control. In
the case of combined (artificial and bacterial fertilizer) treatments we measured lower NO3
--N, organic-N and total-N compared to the
values of plots having only nitrogen fertilizer treatments. On the contrary in the plots with combined treatments the CaCl2 soluble NH4
+-N
content of soil in more cases were higher than that of values with artificial fertilizer treatment.
As a function of calcium-nitrate application increased AL-P2O5 and AL-K2O values were measured compared to control. Microbion
UNC supplement of calcium nitrate yielded also increase in AL-P2O5 and AL-K2O values, till then supplement of ammonium-nitrate fertilizer
yielded a decrease in these values compared to the control.
All nitrogen fertilizers resulted in a significant decrease in AL-Mg content of soil compared to the control. Nevertheless bacterial
fertilizer increased AL-Mg values in any cases. -
Application of the Collision Cell (CCT) in the ICP-MS Analytical System
120-125Views:593Our laboratory has seen a sharp rise in the number of requests for the analysis of smaller and smaller concentrations of elements from foods, plants, soil, organic fertiliser, irrigation and ground water, sewage, sewage sludge, raw material of food, as well as human and animal origin samples. From the above elements the inductively coupled plasma mass spectrometer is capable of analysing the smallest concentration of elements. Our method of analysis is to use our ICP-MS instrument, together with a ICP-MS with Collision Cell Technology (CCT). The CCT method has better detection limit, with 1-3 magnitudes, compared to the normal ICP-MS analytical method. The CCT has better detection limits mainly for the following elements in the periodical system: analysis of arsenic, selenium, germanium, vanadium and chromium. Additionally a collision cell can be applied for the analysis of silicium, sulphur, zink, copper, iron, calcium, magnesium and potassium in smaller concentrations.
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Comparative studies to model bioavailability of pesticides in distinctive soil types
17-23Views:162Bioavailability of pesticides is determined by two major factors: soil characteristics and pesticides’ chemical feature. These factors result in a definite adsorption capability whose extent varies on a large scale. By revealing interactions between pesticides and soils it is of high interest to model bioavailability of widely used pesticides, as it is a key element in terms of prospective toxicological aspects. Our work signifies steps forward improving pesticide soil mobility prediction models as we created model systems representing correctly natural relations. Comparison of different solvent extraction methods proved to be an efficient tool to gain information on the bioavailability of some widely used pesticides as well as to model actual environmental processes.
Comprehensive comparison has been made between different experimental methods by applying 5 extraction models showing diverse efficiency in extracting capability of pesticides. In some cases chloroform excelled in mobilizing pesticides from soil, however mostly application of humic acid solution as extraction model was found to be at least as efficient as methanol, chloroform or CaCl2-solution.
Four chemically much different pesticide (simazine, acetochlor, chlorpyrifos and diuron) were applied to two soil types (both sandy and brown forest). The extracted amounts were determined by GC/MS technique. Adsorption coefficients (Kd) were also calculated for the examined samples.
Obtained results for Kd indicated that chemical feature of pesticides seemed to be of utmost importance in terms of soil binding capability preceding the relevance of soil characteristics. Adsorption capability of chlorpyrifos proved to be the most pronounced preceding simazine and the least prone to bind to soil acetochlor and diuron -
The precision livestock farming
201-202Views:341The application of information technology is the response of the livestock farming to the demand of customer, legal and economical expectations. This technology is the socalled precision livestock farming (PLF). The elements of the PLF are: continuous monitoring of inputs, animal behaviour by sensors, an algorithm which converts these signals into a figure, this figure is compared to an optimum then adjustment of the input is followed, if it is necesary.
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Operational experiences of a geothermal heat pump system
115-118Views:232Energy consumption nearly 40% operation translates of the buildings in Hungary. In the last 5–8 years we can see a breakthrough in the field of low energy buildings, increasing the demand for buildings 'energy efficient', and realized several successful investments in our country. Nowadays thanks to the application system the number of public buildings increased environmental energy recovery system has been growing such as solar power, heat pumps.