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  • Using the principles of precision animal husbandry in fishbreeding
    283-287
    Views:
    181

    Aquaculture species such as fish, crayfish, molluscs and plats are a wide range of products, with continuously growing demand worldwide. The reasons for this is that they are cheap and easy-toraise protein sources, thus having significance in food supply especially in developing countries in tropic regions, moreover, the premium category foodstuffs in developed high income countries are also belonging to this category. World annual total production of 164 million tons (2009) are made up of two sources: 1) marine and inland fisheries landings that are stagnating for several years and 2) aquaculture which is growing dynamically with annual 6% rate between 2000–2009. The latter is accounting for nearly 45% of the total supply due to the depleting marine stocks caused by overfishing. Aquaculture is growing continuously also because the production is safe and can
    be planned well. 
    Intensive fish production systems are the representatives of precision animal production, several types exist and widespread worldwide. The modern computerized temperate water recirculation plants with several thousand m3 capacity are widespread also in Europe because they make it possible to produce even the most valuable species whole year round. A key issue in the technical/feeding outlay is to meet the demands of the cultured species the best and the operation of the system is to be safe and cost-effective. One condition for this is intensification: enable to produce more product per unit resource input or effort. The facilities need significant amount of energy, thus renewable energy sources are to be favoured for which Hungary has comparative advantages.

  • Effect of allithiamine on the level of hyperglycaemia-induced advanced glycation end products
    41-44
    Views:
    231

    Diabetes mellitus is a rapidly growing public health burden in both developed and developing countries. Diabetes mellitus is accompanied by hyperglycaemia, which can cause tissue injury by several mechanisms. One of these is the formation of advanced glycation end-products (AGEs). In this study, the effect of allithaimine, a fat-soluble thiamine derivative, was investigated on hyperglycaemia-induced AGEs levels using human umbilical cord vein endothelial cells (HUVECs) as a hyperglycaemic model. HUVECs were isolated by enzymatic digestion, characterized by flow cytometer and treated 30 mM glucose plus allithaimine or thiamine or cell maintenance medium as control.  Allithiamine was synthesized and purified. The structure of the synthesized and isolated compound was verified by reverse phase HPLC and MALDI-TOF. AGEs were evaluated by ELISA. Collectively, our results indicate that allithiamine can reduce level of the hyperglycaemia-induced AGEs similar to thiamine.

  • Comparison of supplier quality assurance methods for compound feed manufacturing from efficiency, product consistency and economical point of view
    29-34
    Views:
    87

    Feed manufacturing and human food production are the main routes of use for agricultural products. The food industryparticularly has intensively implemented the recent quality management principles and developed systems that facilitate the continuous improvement and efficiency of the industrial production. Feed production has taken similar approaches however the intensity of deployment at the manufacturer and the rollout towards its supply chain has shown slower progress. The methods, that the feed manufacturer manages the supplier chain of mainly primer agricultural products according to, have a certain impact on the efficiency of the inbound operations, feed product quality and its consistency as well as on other resources. These methods have been built on sound quality management principles that are stated not only in quality standards but also in relevant regulations. Current study addresses the questions related to the link between supply chain quality management and feed product. The objective of the frontier research was to highlight the  theoretical possibilities and benefits of the robust design methodimplemented into animal feed manufacturing dealing with highly variable ingredients. 

  • Comparison of sample preparation methods for the identification of Staphylococcus Aureus by MALDI-FOF MS
    9-14
    Views:
    270

    Coagulase-positive staphylococci include 3 species, Staphylococcus aureus, S. hyicus and S. intermedius. Of these three species, S. aureus is the most well-known human pathogen. S. aureus is part of the human and animal normal microbiota, however, it is capable of producing several staphylococcal enterotoxins (SEs) that cause intoxication symptoms of varying intensity in humans after consuming contaminated food. Selective media which are used for the determination of coagulase-positive staphylococci from foods are not able to identify isolates at a species. With the MALDI-TOF MS technique, we can identify S. aureus cheaper and faster than by using molecular methods. This paper describes the results of the study of the presence of coagulase-positive staphylococci and S. aureus in many food products, and the application of three sample preparation methods: direct sample preparation, formic acid suspension and ethanol extraction.

  • Mitigation of environment impact of mycotoxins produced by Fusarium species
    159-164
    Views:
    139

    The Fusarium fungi hazards the grain quality of cereals, therefore significantly affects their utilization as animal feed or consumable product. The Fusarium can decrease the quality of wheat in different ways: decreases the germination capability, causes visible discoloration, mould may appear, reduces the dry material and nutrient content of the grain, causes mycotoxin infection – as a result given by its by-product. Micotoxins produced by Fusarium genus, as the trichotecenes (T-2, HT-2, deoxynivalenol, nivalenol, diacetoxyscirpenol, Fusarenone-X) and the zearalenone (F-2) are the most common in Hungary. Occurrence of fumonisins first discovered in 1988 are must be identified carefully. About 20–30% of the overall worldwide production of cereals is infected with Fusarium and its toxins, which situation is similar in Hungary. This infection causes serious yield-losses in cereal production. In the case of cereal products, which non-utilizable as forage seems, an optimal solution is utilizing as biogas raw material, but it is also important to examine the effect of the infected cereal on the anaerobe digestion process.

  • Effects of the development of production factors on productivity
    13-18
    Views:
    119

    In every economic sector, the enacted and expected quality of the products strongly affects its market entry and subsistence. The aim of management is success, competitiveness and subsistence that require adequate human and material resources and enough production stock. We must also notice that the quality of tools and instruments significantly influences work productivity. This applies to both plant production and animal husbandry.

    The author researched milk production factors with methodical observation, document analysis and interviews. Data were collected with a ten-year interval, thus this assay can highlight the field, volume and sources of investments during this period and that the production stock increased in most farms and decreased in few. The data also shows the influence of investments on work productivity - there are differences between farms of equal size, so there are still ways of development.

  • Characteristics of Land Use and Plant Production in Transcarpathia
    290-294
    Views:
    35

    The character of plant cultivation and animal husbandry in different parts of Transcarpathia, are mostly determined by the location of a given region. Usually, four zones are delineated: 1. lowlands; 2. foothills and volcanic remains zone; 3. inter-mountainous subsidence and deep valley; 4. mountain zone.
    In county this zone divides quite visibly. By studying the soil, climatic conditions and plants, it is not difficult to see that the vertical zones play a major role in their characteristics and formations. If we go from lower to higher elevations, we can observe the qualitative difference in the environment and, of course, in the character of agriculture.
    Environmental pollution is a global problem of our age. In agriculture, the most important thing is to preserve the ideas of sustainable development, because only these can ensure the protection of production resources, which will ensure the continuous production of agricultural products. Present environmental conditions pose a more problematic challenge to agriculture.

  • The possibility of increasing at the conjugated linoleic acid content in pork
    27-32
    Views:
    116

    The composition of fatty acids in food products is a significant factor in human health. Feeding can significantly influence the composition of fatty acids in the animal fat. We analysed the effect of feeding high CLA-content (conjugated linoleic acid) feed on the composition of fatty acids in pork. The animals were grouped according to the following: Group 1) feeding experimental, ghee-mixed feed for 76 days, Group 2) feeding the same feed, but only for 33 days, Group 3) feeding sunflower-oil-mixed feed for 76 days. Ghee contains CLA in high amount. The aim of our experiment is to analyse how the high CLA content influences the fatty acid content of pork. In the end of the fattening experiment the animals were slaughtered, then samples were taken from the loin, ham, abdomen and backfat from 10 animals from each group and analysed the fatty acid content. We found significant differences between the average fatty acid content of the samples. As an effect of feeding ghee-enriched feed, the CLA content significantly increased, compared to the control group. However, the linoleic acid and the arachidonic acid content were lower, and the proportion of fatty acids was also lower when feeding control feed.

  • Deproteinized plant juice as part of circular economy: A short review and brief experimental data
    23-26
    Views:
    1485

    As the population of the Earth is constantly growing it generates an unmet demand for protein, which is an urgent problem. The protein extraction process is a potential solution, which offers high-quality plant protein suitable for animal and human nutrition at a favorable price. The process used within our project produces green juice from the green alfalfa biomass through pressing. After the coagulation of protein from this green juice, the by-product is called DPJ (Deproteinized Plant Juices) or brown juice. Our preliminary results match the international literature, namely that brown juice take up as much as 50% of the fresh biomass in weight. To utilize this by-product is a crucial part of the process to make it environmental-friendly and financially viable as well. The examined brown juice samples came from a small-scale experiment of alfalfa varieties carried out in the experimental farm at the University of Debrecen. According to our preliminary results, brown juice has high macro- and micronutrient values, furthermore, it has a potentially high amount of antioxidant compounds. The study highlights that brown juice is suitable as an ingredient in microbiological media, in plant nutrition as a supplementary solution, for feedstock and for preparing human food supplements or functional foods. The potential utilization of all biorefinery products makes it a very appropriate technology for today’s challenges.

  • The effect of breed and stage of lactation on the microbiological status of raw milk
    37-45
    Views:
    298

    The microbiological quality of the milk is important not only for food safety, but it can also influence the quality of dairy products. The microbiological status of raw cow milk can be influenced by many factors. Our aim was to determine whether there was a difference between the microbiological quality of milk of two different cow breeds (Holstein Friesian and Jersey) kept and milked in the same conditions, and how the microbiological quality of the raw cow milk changed during lactation (beginning, mid, and end). Samples were taken and analysed in July, August and September in 2018 from two dairy farms in Hajdú-Bihar county. During the conducted studies, the total plate count (TPC), the coliform count, the Staphylococcus aureus count and the coagulase-negative Staphylococcus (CNS) count of raw milk samples were determined.

    There was no significant difference (P>0.05) between the milk of the Holstein Friesian and Jersey breeds in the case of TPC. However, the mean coliform count of milk samples taken from Holstein Friesian cows was significantly lower (P<0.05) than the mean coliform count of milk samples taken from Jersey cows. S. aureus was detected in one of the twelve milk samples taken from Holstein Friesian cows, and in two of the eleven milk samples taken from Jersey cows. CNS was found in larger amount in milk samples taken from Holstein Friesian cows, and the difference was significant (P<0.05). Both TPC and CNS count were significantly higher (P<0.05) in individual milk samples taken at the end stage of lactation, than in samples taken in the earlier stages of lactation from Farm “A”. However, in the case of Farm “B”, there was no significant difference (P>0.05) in colony counts at different stages of lactation. S. aureus was only present in milk samples that collected from cows, which were at the beginning and middle stages of lactation. Testimg the hemolysin production ability of S. aureus strains isolated from the raw milk samples, only weak hemolysis was observed on blood agar. In case of antibiotic resistance testing, it was found that all strains were susceptible to cefoxitin, chloramphenicol, clindamycin, erythromycin, gentamicin, penicillin G, tetracycline and trimethoprim/sulphamethoxazole.

    Based on the results of our studies, staphylococci were detected in a higher amount in the milk of Holstein Friesian cows, and coliform bacteria were detected in a higher number in the milk of Jersey cows. Summing up the results of the milk samples taken from the different stages of lactation in one of the farms, it can be concluded that higher TPC and CNS count could be detected at the end stage of lactation than in the samples taken from the earlier stages of lactation. The fact that at the end of lactation the microorganisms could be detected in a higher colony count may be related to the fact that teats could be damaged during lactation by the milking machine, which increased the chance of imvading the microorganisms into the udder.