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The effect of β-glucan, carotenoids, oligosaccharides and anthocyanins on bacteria groups of excreta in broiler chickens
15-20Views:270This study was conducted to examine the effect of natural compounds, such as β-glucan, carotenoids, oligosaccharides, and anthocyanins in the diet on bacteria gropus of excreta in Ross 308 broiler chickens. Chickens were fed 5 diets: control (basal) diet, a diet supplemented by β-glucan at 0.05%, and diets supplemented by carotenoids, oligosaccharides, or anthocyanins at 0.5% of each compound. On experimental day 19, excreta were collected to determine the proportion of Lactobacillus, Bifidobacterium, Campylobacter, Clostridium, and Escherichia coli. Samples were collected aseptically and snap-frozen in liquid nitrogen. Bacterial DNA was isolated from samples, then polymerase chain reaction using primer pairs designed to the 16S rDNA of bacterial groups were applied to define the proportion of the mentioned bacteria. Another universal primer pair was used to amplify a region of 16S rDNA of all the examined bacteria. Proportion of each bacterial groups was determined relatively to the intensity of universal PCR product band by gel documenting system and ImageLab software. Based on the results, carotenoids and anthocyanins increased the proportion of Bifidobacterium, which might imply the beneficial effects of the mentioned compounds on the bacteria composition of excreta.
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The effect of long-term fertilization on the 0.01 M CaCl2 extractable nutrient content of a meadow soil
73-79Views:107During my research, I studied the 0.01 M CaCl2 extractable NO3--N, NH4+-N, Norg, P and K contents of the soil samples originated from a long term fertilisation trial in the experimental site Hajdúböszörmény. Relationships among the soil nutrient contents, the agronomic nutrient balances of the 2009 year, and fertilization were studied.
From the results of the study it was concluded as follows:
– Fertilization significantly increased the CaCl2 extractable NO3--N, NH4+-N, and K contents of soil.
– Norg fraction increased as a function of the increasing yield. Hence, it can be assumed that the greater the produced yield, the more the stubble and root residues remain on the arable land. These organic residues can result significant increase in the Norg content of soils.
– The CaCl2 extractable P and K contents were compared with the calculated P and K limit values. According to these, the experimental soil has a good phosphorus and lower potassium supply capacity. These results are in accordance with the results of the conventional Hungarian fertilization recommendation system.
– It can be stated that the 0.01 M CaCl2 is able to determine not just inorganic N forms but Norg fraction as well that characterize the easily mineralizable nitrogen reserves. The results proved that AL-P and -K (ammonium lactate acetic acid, traditional Hungarian extractant) are in good agreement with the P and K reserves, but it is important from the aspect of environmental protection and plant nutrition to measure the easily soluble and exchangeable K-, and P-contents of soil. 0.01 M CaCl2 method is recommended for this. -
Production of corn in Serbia in the light of climate change
305-322Views:211This paper presents the basic elements of maize production technology in Serbia. For their good knowledge, it is necessary to be familiar with the basic biological requirements of the species, phenology and its particular requirements for water, heat, soil conditions and mineral nutrition. A special emphasis in this paper is given to recent research by the author regarding the correction of production technology (selection of hybrids, time and density of sowing, etc.) from the aspect of knowing the weather conditions of the year to the moment of sowing and the amount and position of available nitrogen in the soil profile during the spring before sowing. In the light of predicted climate change conditions for the Republic of Serbia, the authors of this paper have proposed and appropriated adaptation measures. The general conclusion is that there are no general recipes for the correct production technology of this plant species, but should be adapted to each specific year, field and hybrid.
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Evaluation of the microbial soil quality indicators in agricultural soils from Crisurilor Plain
79-80Views:130The researches were carried out in 2010 and 2011 on the haplic luvisol cultivated in three variant such as: pasture, cropland, and orchards. Based on the total number of microorganisms monitored in the haplic luvisol (aerobic mesophilic heterotrophs, yeast and mould, Actinomycetes, nitrogen fixing bacteria and nitrifying bacteria) was calculated the bacterial indicators of soil quality (BISQ) and was appreciate the bacterial potential of the haplic luvisol. Seasonal analyses were carried out, and annual BISQs have been calculated too. The values of the bacterial indicators of the haplic luvisol in different cultivation conditions (pasture, cropland and orchards) indicate a high density of the bacterial groups in 2010 and 2011. In 2010 the values of the BISQs are included between 3.617 (minimum in autumn, in pasture) and 5.458 (maximum, in spring, in pasture). In 2011, the minimum value (3.622) was registered in pasture, in autumn, and the maximum value (4.851) in the haplic luvisol cultivated with maize, in spring. In 2010 and 2011, based on the bacterial indicators of the soil quality values, on the first position is the cropland (4.750, 4.721) followed by orchards (4.615, 3.985) and pasture (4.537, 3.758).
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Effect of agrotechnical factors on the activity of urease enzyme in a long term fertlization experiment
43-48Views:154The soil is a natural resource, the fertility preservation is an important part of the sustainable development. We have to monitor the transformation dinamics of the organic nitrogen-containing substances, to get accurate information about the changes of the nitrogen cycle in the soil.
Physical and chemical properties of the soil and the microorganism effect on the organic matter in the soil – in addition to the composition of organic matter. Wide variety of extracellular enzymes are present in this decomposition. These enzymes help in the transformation of the macromolecules to transforming low molecular weight compounds so they will be available during the assimilation.
The urease enzyme, catalyzes the hydrolysis of urea to CO2 and NH3. The urease is widely spread in the nature, it is present in the microorganisms, plants and animals.
We found that the soil moisture content, the rotation and the fertilization affect to the amount of urease in spring. Furthermore, we get significant difference between the irrigated and non irrigated samples in the second period of the year. Based on our results we can state that the activity of urease was higher in spring 2014.
The objective of our study was to present how the different agronomic factors affect on the activity of urease in a long term fertilizationexperiment.
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Nutrient Uptake of Miscanthus in vitro Cultures
23-24Views:95The large biomass production and the low necessary input fertilizer make Miscanthus an interesting, potential non-food crop with broad applications, e.g. for fuel and energy, for thatching, fiber production, for the paper and car industries, as well as for ethanol production.
Axillary buds of Miscanthus x giganteus were placed on a shoot inducing nutrient solution (modified Murashige and Skoog, 1962), basic medium supplemented with 0,3 mg l-1 6-Benzylaminopurin. After 40 days of culturing, the axillary buds produced three times more shoots than could normally be harvested. The nutrient content (N, P, K, Ca, Mg) was measured several times during culturing. The results showed that, after 35 days, nitrogen and phosphate were nearly completely taken up. From that time, shoot growth was not observed.
After shoot propagation, the plants were transfered into a nutrient solution for root formation (modified Murashige and Skoog, 1962), basic medium supplemented with 0,5 mg l-1 Indole- 3-Butyric acid, and could be potted in soil after about 14 days. -
Changes in toxic elements content of soil after sewage sludge treatment in energy willow plantation
7-10Views:165The primary purpose of our experiment was the solution of municipal excess sludge treatment by a renewable energy resource used willow (Salix viminalis L.) plantation. Tests were carried out to state whether the applied sewage sludge has caused any accumulation of the toxic elements in the studied soil layers, and - based on the results –to see whether the plantation is suitable for the treatment of municipal sewage sludge.
The excess sludge (sludge before dewatering) is beneficial for the willow, because it contains a 3–5% dry matter and therefore, a lot of water, too. This high water content ensures the high water amount needed for the intensive growth of the willow. On the other hand, the wastewater treatment plant can save the dewatering cost which corresponds to about 30% of the water treatment process costs. The amounts of the sprinkled sewage sludge were calculated on the basis of its total nitrogen content. Treatments were the followings: control, 170 N kg ha-1 year-1 and 250 N kg ha-1 year-1. The mean values of the toxic element concentrations in the sewage sludge did not cross the permitted limits of the land accommodating.The measured toxic element values of the soil were compared to the limits of the 50/2001. (IV.3.) Government Regulation.The sprinkled sewage sludge on the bases of the total N content did not cause accumulation of heavy metals in the soil and the treated plants were also healthy without any signs of toxicity.
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Visualization techniques in agriculture
95-98Views:174This paper describes a dynamic map representation method which provides a flexible, spectacular and cost-effective opportunity for the illustration and description of spatial data due to its parametrability, web-based publication and the free sowftare it uses in multi-user circumstances.
The tasks of the database serves and the processing were performed by an ASUS WL-500 G Premium v2 router and a 80 GB hard disk. The database contains the measured data of the nitrogen fertilisation experiment established on the Látókép Experiment Site of the Centre for Agricultural Sciences of the University of Debrecen. The tests showed that the generation time of the processor which was run through the router did not significantly increase. Therefore, the configuration developed by us is suitable for users who do not wish to invest into a large and expensive server, but they still want to view their data quickly and easily, as well as to reach them from anywhere.
The available data were not sorted into a database which was performed with Quantum GIS in a way to have an optimum database structure which is adjusted to the expected areas of use and the expected running speeds were also taken into consideration. The processor which processes the database items was written in PHP language. The main role of the processor is that it produces a KML file real time which is suitable for viewing with a given map viewer client (e.g. Google Earth). This application makes it possible to view information related to geographical objects, values stored in the database or those calculated by the processor on a map in 2D or 3D in a versatile way.
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15N-Tracertechnik in der Pflanzenbauforschung
3-9Views:82Issues of nitrogen are still of particular importance in crop sciences. 15N-tracer are used to identify the N dynamics in soils and the N transfer between soil and plant. This tool is also helpful in clarifying fertilization problems.
This article points out the special requirements for the application of stable 15N-isotops in agri-chemical research. Designs and results of selected laboratory experiments, examples of one-year field experiments, and a 15N long-term trial are represented in detail. The given literature refers to detailed results of diverse 15N publications in the system soil – fertilizer – (animal) – crop. -
Results of a Fertilization Experiment Performed with Sulphur on Rape Seed Plants
116-119Views:157The sulphur fertilizing experiment was introduced in the cropping year of 2001/2002, with winter rapeseed. The experiment was performed on a farm in Magyarhomorog, Hajdú-Bihar County. By selecting the location for the experiment, we had to consider the effect of the hard winter that was very unfavourable for rapeseed production, as there was serious frost damage on the sown area previously used for the experiment. In the arable land experiment, results of three different doses of treatment were compared in two replicates, on meadow soil. FitoHorm 32 S solution was used as a sulphur fertilizer, in doses of 3, 6 and 10 l/ha. Evaluating the results, we tried to find a correlation between the amount of fertilizer and the amount of seed-crop or the oil content of the seed. The nitrogen and sulphur contents of the samples were determined using classical methods and an Elementar VarioMax analyser. By this way, it was possible to examine not only the role of Sulphur in rapeseed production, but also to compare and evaluate results obtained by Kjeldahl and the modern, environmentally-friendly combustion method.
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Examination and statistical evaluation of physico-chemical parameters of windrow composting
33-38Views:263The treatment and utilization of plant and animal waste and by-products from agriculture is very diverse. Traditional environmental management practices for waste management have been retained through soil conservation and the applied of recycle degradable organic substances in soil. The management of by-products from agriculture (animal husbandry) is important because a closed loop can be created to utilize by-products (manure, feathers) from the production of the main product (eggs, meat, milk) and to form a raw material for a new product. It is important to treat the resulting by-products, especially deep-litter manure, as it has served as a basis for compost-treated manure to develop an organic-based, soil-conditioning product line. Poultry manure by itself is not suitable as a substrate for aerobic decomposition, so it has to be mixed with other substances (zeolite, bentonite, soil), because of its high nutrient capacity, it is an acidifying substance.
The aim of this study was to compost the mixture of poultry manure and hen manure by the addition of zeolite and to monitor the composting process. It was also our aim to statistically determine the effect of the zeolite on parameters describing the composting process.
The windrow composting experiments were set up in the composting area of the University of Debrecen, Institute of Water and Environmental Management. The composting experiment was 62 days long, during which the main parameters describing the composting process were continuously monitored: temperature (°C), moisture content (w/w%), electrical conductivity (mS/cm), organic matter content (w/w%), examination of nitrogen forms (w/w%). In this study, three factors were investigated: temperature, humidity, and pH. For statistical evaluation, R software and RStudio user interface were used. We developed a repeated measurement model, in which the fixed and random effects were determined for our parameters under study, and the resulting relationships were shown on interaction plots.
Based on our results, the temperature of the prisms has become independent of the ambient temperature and the composting stages can be separated in both the control and the zeolite treated prisms. In the repeated measurement model, we proved that treatment, time and treatment: time interaction were significant at both temperature and pH.
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Activity of some enzymes, participating in nitrogen compounds transformation in chernozem, polluted by fluorine compounds
99-104Views:126Contamination of chernozem by fluorine compounds variously affects those enzymes (urease, asparaginase, glutaminase, arginase, amidase), which takes part in the metabolism of nitrogen-bearing organic compounds. In broken soils the inhibited desaminisations is stronger, than enzymatic hydrolysis of asparagine and arginine. The features of seasonal dynamics of change activity of urease and correlation dependence of its activity from some physical and chemical soils properties are described. These tendencies well comport with the results of model experiments. At minimum HF influence there is inhibition of processes of monohydrocarboxylic acids desaminisation, hydrolytic breaking up of arginine and glutamine. By a side with this there is activating of urea and asparagine breaking up processes on the initial stages of toxicant influence. The study of kinetics of process of urea enzymatic hydrolysis in chernozem at the different level of HF influence showed changes of initial and maximal velocity of enzymatic reaction, and also Michaelis-Menten constant.
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Effect of cropping technologies on the yield of dry bean variety ’Diana’
37-41Views:144An experiment on three dry bean varieties (Start, Hópehely, Diana), using different sowing-times, fertilizers and plant densities was performed on sandy soil in the University of Debrecen, Centre for Agricultural Sciences, Research Institute of Nyíregyháza in 2015. The aim of the experiment was to study which treatment gives the highest yield, and whether the different treatments result in significant differences in the yields. In this paper the dry bean yield at ‘Diana’ variety is analyzed.
The treatments were done with three fertilizer doses and three plant densities at different sowing-times (April 24; May 8; May 18). As a result of the high temperature and the drought during the growth season, the yields we harvested were in low, which shows the ecological sensitivity of the plant we examined.
We concluded that the poorest yield was harvested at the third sowing-time. There was no significant difference in the yields at the first and second sowing- time. Examining all the three fertilizer treatments we applied at the experiment, we achieved the highest yields in the control plots. It might be due to the weak efficiency of nitrogen fertilizer under the extremely dry conditions. The highest yield was harvested at the control treatments during the second sowing-time. Regarding the effect of the plant densities, the highest crop yield was achieved at the treatment using 400 000 germs ha-1, followed by 300 000 germs ha-1 and 200 000 germs ha-1.