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Examination of repeatability of ultrasound scanning technique in young bulls
43-48Views:129The author’s goal with the methodology examination was to determine repeatability of taking and evaluating ultrasound images. In the trial an operator person took two images about the ribeye area and rump fat thickness (P8) of every young bull, which were measured by the same person in four repetitions. Also, we had altogether eight images about the same part of the body. Images were collected using Falco 100 (Pie Medical) real-time ultrasonic scanner equipped with an ASP 3,5 MHz, 18 cm linear array transducer.
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Development of seed analyses by means of various matrix solutions and the MALDI-TOF MS technique
53-57Views:294The earth's population is growing steadily, currently accounting for about 7.3 billion people. Population growth causes food demand to rise, approximately 36 million people die each year due to starvation or related diseases. One solution to this problem is the continuous examination and development of the agricultural economy. In this study, matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI -TOF MS) were used to analyse of sunflower, soybean and hemp. In order to analyse the protein of maize, this method has already been applied. However, for sunflower, soy and hemp, it is necessary to develop a sample preparation method. Choosing the optimal matrix solution for ionization the traget molecule is an essential part of developing the method. Our aim is to compare two different matrix solutions (α-HCCA, SA matrix), based on the properties (intensity, noise ratio, value of spectra) of the spectra.
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Studies on the Fusarium stalk rot infection of the maize genotypes using the Findex percentage and a computerised image analysis program
45-51Views:104In a continental climate, the pathogens causing the most serious problems are species belonging to the Fusarium genus. When the pathogen attacks the stalk, the plant dies earlier, reducing grain filling and resulting in small, light ears. In addition, the stalks break or lodge, resulting in further yield losses from ears that cannot be harvested. During the three years of the experiment, 14 inbred lines were examined. The genotypes were sown in a two-factor split-plot design with four replications, with the genotypes in the main plots and four treatments in the subplots: two Fusarium graminearum isolates (1. FG36, 2. FGH4), 3. sterile kernels, 4. untreated control. The results experiments showed significant differences between the genotypes for resistance to fusarium stalk rot. Among the inbred lines the best resistance to fusarium stalk rot was exhibited by P06 and P07, both of which were related to ISSS. The precision and sensitivity of disease evaluations carried out visually and using image analysis software were compared in the experiment, and with two exceptions the CV values were lower for the image analysis. As the CV for measurements can be considered as a relative error, it can be stated that image analysis is the more precise of the two methods, so this technique gives a more accurate picture of the extent of stalk rot. The extent of stalk rot developing in response to natural infection is extremely environment-dependent, so the use of artificial inoculation is recommended for selection trials.
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Comparative studies to model bioavailability of pesticides in distinctive soil types
17-23Views:83Bioavailability of pesticides is determined by two major factors: soil characteristics and pesticides’ chemical feature. These factors result in a definite adsorption capability whose extent varies on a large scale. By revealing interactions between pesticides and soils it is of high interest to model bioavailability of widely used pesticides, as it is a key element in terms of prospective toxicological aspects. Our work signifies steps forward improving pesticide soil mobility prediction models as we created model systems representing correctly natural relations. Comparison of different solvent extraction methods proved to be an efficient tool to gain information on the bioavailability of some widely used pesticides as well as to model actual environmental processes.
Comprehensive comparison has been made between different experimental methods by applying 5 extraction models showing diverse efficiency in extracting capability of pesticides. In some cases chloroform excelled in mobilizing pesticides from soil, however mostly application of humic acid solution as extraction model was found to be at least as efficient as methanol, chloroform or CaCl2-solution.
Four chemically much different pesticide (simazine, acetochlor, chlorpyrifos and diuron) were applied to two soil types (both sandy and brown forest). The extracted amounts were determined by GC/MS technique. Adsorption coefficients (Kd) were also calculated for the examined samples.
Obtained results for Kd indicated that chemical feature of pesticides seemed to be of utmost importance in terms of soil binding capability preceding the relevance of soil characteristics. Adsorption capability of chlorpyrifos proved to be the most pronounced preceding simazine and the least prone to bind to soil acetochlor and diuron -
Comparison of sample preparation methods for the identification of Staphylococcus Aureus by MALDI-FOF MS
9-14Views:281Coagulase-positive staphylococci include 3 species, Staphylococcus aureus, S. hyicus and S. intermedius. Of these three species, S. aureus is the most well-known human pathogen. S. aureus is part of the human and animal normal microbiota, however, it is capable of producing several staphylococcal enterotoxins (SEs) that cause intoxication symptoms of varying intensity in humans after consuming contaminated food. Selective media which are used for the determination of coagulase-positive staphylococci from foods are not able to identify isolates at a species. With the MALDI-TOF MS technique, we can identify S. aureus cheaper and faster than by using molecular methods. This paper describes the results of the study of the presence of coagulase-positive staphylococci and S. aureus in many food products, and the application of three sample preparation methods: direct sample preparation, formic acid suspension and ethanol extraction.
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Cluster, a potential tool for rural development
195-201Views:157There was a heavy change in the characteristics of the agriculture in the last 20 years. It become a multifunctional, sustainable, organic system, which needs strategic approach. On the problem map of the hungarian agriculture the most importatnt questions are the following: dual charachter of the land structure, to occidentalize the farm structure. To develop the rural areas we need to real markets needs adapted, competitive, local agricultural production. From the ’90s into Europe’s economic development policies integrated the clusters, this research tries to certify, that this system is viable in classic agriculture and able to dissolve the defecinces, to support the aims of rural development.
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Interaction of yield stability and year in major agricultural crops
41-46Views:92The effect of hydro-meteorological extremities on plant cultivation is the result of the correlation of many factors. These may increase or decrease the effects of hydro-meteorological extremes. The degree of this variance depends on the professionality of treatments, on the quality of the applied technique and technology and also on the soil’s water management characteristics.
The water management characteristics of Hungary’s arable land are mainly unfavorable or medium. In the past two decades the conditions of originally good soils, from a water management aspect, have significantly deteriorated in the critical 0-60 cm soil layer. This is mainly due to unprofessional land use, a lack of deepening cultivation and neglected organic cultivation. At the same time, hydro-meteorological extremities occur more frequently and the sensibility of plant cultivation has increased.
The sensibility of plant cultivation is type and location specific, yet, it also effects both the quality and quantity of the result.
The stability analysis, which covered the period of four decades and incorporated 6-7 agro-ecological areas proves and highlights the following:
• Winter wheat only reacted to extensive cultivation and unfavorable environmental conditions to a small degree. On the other hand, the effect of hydro-meteorological extremities increases.
• The stability analysis of maize, which is sensitive to cultivation technology and the location of cultivation, proved just the opposite. Good soil and adequate technology significantly reduces the effect of any particular year.
• From the years examined, the most favorable proved to be the one with average precipitation. Maize reacted to both extremities in a similar way. Winter wheat reacted to more precipitation with less yield.
• The yield quality of winter wheat was negatively effected by drought. The negative effect of precipitation is limited to the period of ripening and harvest, so the likelihood of such an effect is not significant.
• The yield of sunflower – due to pests – significantly reduces in years with high precipitation, while a difference between dry and average years cannot be pinpointed out. The oil content in both dry years and in years with high precipitation is evident, compared to years with average precipitation.
• The root yield of sugar beet is reduced by drought while the sugar content depends on soil characteristics and climatic extremities. A difference could also be noted by location, whether in Western Hungary and on the Great Plain. Great sugar content can be achieved in years with high precipitation in Western Hungary, while the same result occurred with average precipitation on the Great Plain. Drought did not have a positive effect on sugar content in either location. -
GC-MS studies to map mechanistic aspects of photolytic decomposition of pesticides
11-16Views:82Transformation of pesticides in the environment is a highly complex process affected by different factors. Both biological and physical-chemical factors may play a role in the degradation, whose ratio depends on the actual environmental conditions.
Our study aims to reveal specific details of photolytic degradation of pesticides as important soil contaminants. Significance of these studies is enhanced by the fact that pesticide decomposition may contribute to soil degradation, and have harmful biological effects by degrading to toxic products. The toxicity of the examined pesticides is well known, however very little information is available regarding their natural degradation processes, the quality, structure and biological impact of the degradation products.
The photolytic degradation of frequently applied pesticides of distinctive types (acetochlor – acetanilide, simazine – triazine, chlorpyrifos – organophosphate, carbendazim – benzimidazole) was investigated. A special, immerseable UV-light source was applied in order to carry out photodegradation. The degradation processes were followed by thin layer chromatography (TLC) and mass spectrometry coupled with gas chromatography (GC/MS). EI mass spectrometry was used to identify the degradation species.
Each of the studied pesticides underwent photolytic decomposition, and the detailed mechanism of photolytic transformation was established. At least four degradation species were detected and identified in each case. Loss of alkyl, alkyloxy, amino-alkyl and chloro groups might be regarded as typical decomposition patterns. Deamination occurred at the last stage of decomposition. -
Definition antioxidant activity of selenium-enriched food sprout, as well as their microbiological analysis
25-30Views:228In this present study, we prepared selenium-enriched food sprouts, where the antioxidant capacity was analysed, we also determined their microbiological status. We took into account the fact, we choose micronutrients to our treatment, that selenium can be delivered to the body by a small amount with the most widely consumed food.
We focused during our research to determine that the increasing concentrations of selenium treatment, in which we used sprouts, knowing fully well that it has an impact on aboriginal antioxidant capacity of sprouts, which is mainly due to high vitamin content of sprouts.
Furthermore, we think it is important to make microbiological analysis, because germination conditions, for example temperature, pH, all this will create an ideal environment for the growth of microorganisms. So we had goal to determine, how the used selenium concentration affect the total plate count, coliform bacteria count and Staphylococcus aureus count of sprouts.
We determined the aboriginal water-soluble and lipid-soluble antioxidant capacity of sprout with the PHOTOCHEM chemiluminometer and we applied pour plate technique for the mapping of the mycrobiological state of sprouts.
Experimental results are evaluated, we state that increasing concentrations of selenite or selenate treatment, is primarily water-soluble antioxidant capacity of sprouts was affected. The water-soluble antioxidant capacity of wheat sprout was much higher than the measured values in pea sprout, this may be linked to what we measured. That is much higher ascorbic acid content in case of wheat sprout, which is well known as one of the most important antioxidant properties compounds of wheat sprout.
We conclude from the results of the microbiological, that the highest concentrations of selenite or selenate treatment has a relative significant anti-microbial effect in case of wheat sprouts. Coliform and total plate count showed no clear decreasing tendency, although the values of treatments in both cases obtained were below the control values.
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Spatially Discrete GIS Analysis of Sampling Points Based on Yield and Quality Analysis of Sugar Beet (Beta vulgaris L.)
32-37Views:89Fulfilment of the increasing quality requirements of sugar beet production can be analysed with sampling of plants and soil at the cultivated area. Analyses of the spatial characteristics of samples require exact geodetic positioning. This is applied in practice using GPS in precision agriculture. The examinations were made in a sample area located in north-western Hungary with sugar beet test plant. According to the traditional sample taking procedure N=60 samples were taken in regular 20 x 20 m grid, where besides the plant micro and macro elements, the sugar industrial quality parameters (Equations 1-2) and the agro-chemical parameters of soils were analysed. Till now, to gain values of mean, weighted mean and standard variance values, geometric analogues used in geography were adapted, which correspond to the mean centre (Equation 3), the spatially weighted mean centre (Equation 4), the standard distance (Equation 5), and the standard distance circle values. Robust spatial statistical values provide abstractions, which can be visually estimated immediately, and applied to analyse several parameters in parallel or in time series (Figure 1). This interpretation technique considers the spatial position of each point to another individually (distance and direction), and the value of the plant and soil parameters. Mapping the sample area in GIS environment, the coordinates of the spatially weighted mean centre values of the measured plant and soil parameters correlated to the mean centre values showed a northwest direction. Exceptions were the total salt and calcium-carbonate contents, and the molybdenum concentration of the soil samples (Table 1). As a new visual analysis, the spatially weighted mean centre values of the parameters as eigenvectors were projected to the mean centre values as origin. To characterize the production yield, the raw and digested sugar contents of the sample area, the absolute rotation angles of the generated vectors were determined, which indicate numerically the inhomogenity of the area (Figure 2). The generated spatial analogues are applicable to characterise visually and quantitatively the spatial positions of sampling points and the measured parameters in a quick way. However, their disadvantage is that they do not provide information on the tightness and direction of the spatial correlation similarly to the original statistical parameters.
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Age of puberty and conception rates of Hungarian Merino, (Merino x Somali) F1 and (Merino x Barbados Blackbelly) F1 ewes
15-19Views:189The aim of our examination was to detect the puberty of the Hungarian Merino and its hairsheep crossbred ewes, and also to determine their average conception rates after having ram exposed in May and June. Hormon diagnostation was used to evaluate the exact date of the very first ovulation. Ultrasound technique was used for checking the conception rates of ewes. The pregnancy detection was applied after a month of the date of taking off the ram.
We evaluated the ages of ewes at puberty. Statistical deviation and standard error were calculated. The results proved the Hungarian Merino x Somali (N=10), ((x ± s) = 173 ± 43) and also the Hungarian Merino x Barbados Blackbelly crossbred genotypes (N=7), ((x ± s) = 186 ± 19) have their puberty much earlier as compared to the fullbred Hungarian Merino ewes (N=10), ((x ± s) = 231 ± 95). We experienced the highest conception rate in the group of the (Hungarian Merino x Somali) F1 ewes after exposing the ram in the „out-of season” period of time. -
Relation of availability and barley uptake of some potentially toxic elements
7-10Views:71A small-plot microelement load field trial was set up on brown forest clay soil with eight elements (Al, As, Cd, Cr, Cu, Hg, Pb, Zn), on 3 levels each (0/30, 90, 270 kg element ha-1). The soil was treated with soluble salts of elements once at initiation (1994). In the seventh year of the experiment (2001) winter barley was the test plant. The total element content was determined in plant samples (shoot, straw, grain) after microwave digestion using cc.HNO3+cc.H2O2. The element composition of the prepared samples was determined using ICP-MS technique. In the experiment toxic effects of treatments and yield loss could not be observed. Zn and As contents in barely shoots were only moderately increased by increasing microelement loads. Effects of Cr, Cu, Hg, Pb and Al treatments could not be observed. On the other hand, Cd accumulation was significant in the shoot. Cd content was also increased both in straw and grain. Results of this experiment prove that Cd remains mobile in the soil-plant system for a long time. Its accumulation can be observed both in vegetative and reproductive parts of plants without toxic symptoms and yield loss.
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Managing risk using real options in company’s valuation
125-132Views:145The valuation of company is very important because provides information about the current value/situation of company, and through this, provide the opportunity of choosing the best company’s growth alternatives. The future strategic decisions are characterized by lack of knowledge, information, so all measures of company’s growth are closely linked with uncertainty and risk. The company’s valuation process is also related with uncertainty and risk. The risk may result both from the assessed assets and the technique used. In literature, we could find three approaches for risk management: capital budgeting based method, methods based on portfolio analysis and real options approach of risk management. Among them, the real options based methods is the most revolutionary approach for risk management. The advantages of the method, consists in the fact, that the process of establishing strategic decisions integrates the possibility of reversibility, delay and rejections, which isn’t it possible at two previous methods. The method also takes into account the total risk of company, so both the company-specific and systematic risk. In this study, I have used one of the best-known real option based method, the Black-Scholes model, for determining the option’s value. Determination of option value is based on the data of enterprise, which was tested Monte Carlo simulation. One of the basic assumptions of the Black-Scholes model is that the value of option is influenced by several factors. The sensitivity of option’s value could be carried out with so-called “Greeks”.. In the study the sensitivity analysis, was carried out with indicators Delta (Δ), Gamma (Γ) and Vega (ν). The real options based risk management determinations were performed in the R-statistics software system, and the used modules are 'fPortofio' and 'mc2d'. By using of real options method, I have calculated the average value of company capital equal with 38.79 million. By using simulation was carried out 1000 runs. The results of this show a relatively low standard deviation, small interquartile range and normal distribution. In the calculation of indicator Delta, could be concluded the value of company moves in 0.831 proportion to the price of options, the standard deviations of index is low, so the real option based method could be used with success in company’s value estimation. The Gamma index shows the enterprise value is sensitive just for large changes. The result of Vega reflects the value of option, so the company’s value volatility, which is small in this case, but this means a volatility of value. In summary, we can conclude that the call options pricing model, well suited for the determination of company’s value.