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Evaluation of Origin and Conformation of Excellent Cows with Long Productive Lives
13-17Views:336In the last few decades a new intensive milking stock has developed as a result of breed-converter crossing in Hungary. Beside this reproduction biology of the population was affected adversely by the keeping-, and feeding technology. Serviceable lifetime has shortened and service period has lenghtened. Whereas profitable milk production needs cows with longer productive life, larger life-time production. In our work we analysed the productivity-, and reputation parameters of the 200 highest lifetime-productivity cows of Hungary. We found that inheritance has not got important role in the serviceable lifetime. Adaptability and good constitution of Holstein-friesian is proved by the fact that cows with large life-time production are reared in large plants, some of them in large stocks, industrial environment. We found during the analysis of reputation parameters that these prominent cows mostly have deeper body, higher dairy form, higher rear udder, smaller foot angle. We found some weak connection between the milk production and the reputation. Loose correlations between useful material content and some linear traits were found.
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The effect of keeping technology on the microbiological status of raw milk
67-75Views:522The importance of the quality of raw milk increased after Hungary had joined to the EU. On delivery of raw milk, the microbiological quality, especially total plate count of the milk is very important. Twenty-two farms (7 large, 4 medium-sized, and 11 small farms) were included in the study. We considered the different farm size, keeping- and milking circumstances during the selection of farms. The examined large farms use loose housing system (cubicle, deep litter) and milking parlour. Most of them use preand post-milking disinfection. In the medium-sized farms, loose,
deep litter and tie-stall housing system, as well as milking parlour, pipeline milking and bucket milking occurred. All of them use preand post-milking disinfection. Small farms use tie-stall housing system, bucket milking and udder preparation by water. Unfortunately, they do not use pre- or post-milking disinfection. In the large and medium-sized farms mainly Holstein Friesian, in the small farms Hungarian Simmental breeds can be found.
The aim of our research was to examine the microbiological status of the raw milk produced in dairy farms (total plate count, coliform count, Escherichia coli count, Staphylococcus aureus count, psychrotroph bacteria count, furthermore yeast and mold count); sources of the contamination; connection between the microbiological quality of produced milk and housing-, milking technologies of farms; furthermore the hygienic circumstances of milking and milk handling of the farms, by the examination of coliform bacteria and Escherichia coli contamination.
During the examination of the connection between the different farm sizes, various housing- and milking forms and the microbiological characteristics we observed similar tendencies in the case of total plate count, coliform count, yeast and molds count, furthermore psychrotroph bacteria count. The value of these parameters was significantly higher in small farms, and infarms which use tie-stall housing forms, bucket milking, udder preparation with water, and which do not use pre- and post-milking disinfection.
The results showed that besides cooling, the milking procedure and the type of udder preparation had the largest effect on the total plate count. Statistical analysis shows that in medium and small farms the combination of pipeline milking – tie stall housing system – disinfectant preparation of the udder; in large farms the combination of milking parlour – loose cubicle housing system – dry preparation of the udder are the most appropriate in the aspect of the total plate count. We experienced that in farms where the hygienic instructions are not followed – and therefore
equipment used during the milking and handling of milk is very contaminated – or rather the separation of mastitic cows’ milk is not appropriate, different microorganisms may contaminate the produced milk. -
Examination of repeatability of ultrasound scanning technique in young bulls
43-48Views:421The author’s goal with the methodology examination was to determine repeatability of taking and evaluating ultrasound images. In the trial an operator person took two images about the ribeye area and rump fat thickness (P8) of every young bull, which were measured by the same person in four repetitions. Also, we had altogether eight images about the same part of the body. Images were collected using Falco 100 (Pie Medical) real-time ultrasonic scanner equipped with an ASP 3,5 MHz, 18 cm linear array transducer.
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Additional data for the evaluation of coat colour varieties in the Hungarian Grey cattle
44-47Views:402When preserving genetic resources, one of the most important tasks is to conserve as much of the given gene pool for the future generations as we can. Therefore, traits that have no economic value at the moment should also be conserved. The great variety of coat colours seen in the Hungarian Grey cattle form part of the world’s genetic heritage. In order to maintain the world’s genetic diversity, we have to maintain these varieties, as well. The different coat colour varieties were determined – in both sexes and in several age groups – with a Minolta Chromameter CR-410 in an objective way. We found that the rate of the three main coat colour varieties of calves: the light reddish, the reddish and the dark reddish were 26%, 52% and 22%, respectively. Statistically significant differences were found between the L*a*b* values of the reddish coat colour of the Hungarian Grey and the red coat colour of the Limousin calves. The rate of the crane, the grey and the light grey coat colour varieties were determined in the measured female groups. The L*a*b* values of the Hungarian Grey and Maremman bulls’ coat were compared and evaluated.
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Horn and coat color varieties of the Hungarian grey cattle
44-48Views:411Due to the intensification of agricultural production, genetic diversity has been reduced to a large extent. Presently, in the period of worldwide genetic conservation, we try to preserve as much of the gene pool of our valuable indigenous domestic animal breeds as we can. Therefore, traits that have no economic value at the moment should also be conserved. The different horn and coat colour varieties of Hungarian Grey Cattle are such valuable traits. Research has been done on the largest Hungarian Grey Cattle stock, at the Hortobágy Kht. Rates of the different horn and coat colour varieties were determined and relationships were analyzed between the above mentioned qualitative traits
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Inner pelvic measurements in dairy breeds
51-56Views:446Dystocia causes great financial losses: due to dystocia milk production is decreased and the probability of calf loss is increased. There are many factors that may cause dystocia. One of the factors –often investigated in beef cattle- is pelvic measurements. There have not been inner pelvic measurement comparisons in dairy breeds in Hungary.
After comparing the imported, primiparous cows, Jersey turned out to have the smallest absolute inner pelvic measures. According to their age and weight, Brown Swiss cows had the largest pelvic dimensions. Ayrshires, Norwegian and Swedish Red, the three dairy breeds which share similar genetic background did not differ in most measures. Holsteins were closest to the seemingly ideal 1:1 horizontal and vertical diameter ratio; however this breed suffers the most from dystocia. Jerseys, despite having the smallest pelvic area are famous of their calving ease, perhaps not by coincidence. Though this dairy breed is the lightest, when pelvic area was compared in ratio of body weight Jerseys were not smaller than the 130 and 114 kg heavier Norwegian and Swedish Red cows. -
Inner pelvic measurements in dairy breeds
31-35Views:403Dystocia causes great financial losses: due to dystocia milk production is decreased and the probability of calf loss is increased. There are many factors that may cause dystocia. One of the factors –often investigated in beef cattle- is pelvic measurements. There have not been inner pelvic measurement comparisons done on dairy breeds in Hungary.
After comparing the imported, primiparous cows, Jerseys turned out to have the smallest absolute inner pelvic measures. According to their age and weight, Brown Swiss cows had the largest pelvic dimensions. Ayrshire, Norwegian and Swedish Red, the three dairy breeds which share similar genetic background did not differ in most measures. Holsteins were closest to the apparent ideal 1:1 horizontal and vertical diameter ratio; however, this breed suffers the most from dystocia. Jerseys, despite having the smallest pelvic area are famous of their calving ease, perhaps not by coincidence. Although this dairy breed is the lightest, when the pelvic area was compared in ratio of body weight Jerseys were not smaller than the 130 and 114 kg heavier Norwegian and Swedish Red cows. -
Comparative analysis of Staphylococcus aureus strains by molecular microbiology methods
34-39Views:560Staphylococcus aureus is a very important pathogen for dairy farms and milk processing plants. Subclinical mastitis is often caused by this species, and it can contaminate bulk tank milk when milking cows are suffering from mastitis. Additionally, thermostable enterotoxins (SE) produced by some types of this bacterium can cause food poisoning.
The aim of our research was to examine the number of S. aureus in bulk tank milk in two dairy farms and the enterotoxin-producing ability, genetic relation (pulsotype) and antibiotic resistance of S. aureus strains from different sources (bulk tank milk, udder quarter milk and environment).
The results show that the mean number of S. aureus of bulk tank milk of two farms significantly differed (P<0.05). Fourteen isolates were selected for further molecular genetic studies (five isolates were from bulk tank milk and nine isolates were from udder quarter milk). S. aureus was not recovered from the environmental samples. Three of the fourteen isolates (21.4%) tested by multiplex PCR were positive for SE genes. Two isolates carried one gene (seb) and one isolate carried two genes (seg and sei). The fourteen strains were classified into three pulsotypes and two subtypes at 86% similarity level. Isolates from bulk tank milk (n=5), were divided into 2 pulsotypes (A, C) and one subtype (C1). The isolates from udder quarter milk (n=9) belonged to three different pulsotypes (A, B, C) and two subtypes (A1, C1). The distribution of pulsotypes in the present study revealed genetic relationship between S. aureus isolated from udder quarter milk and bulk tank milk. This could be explained by the fact that in farms with a high number of infected cows, these cows could represent the main source of contamination. The results of the antibiotic resistance investigations show, that all strains were susceptible to methicillin, cefoxitin, lincomycin, tetracycline, erythromycin and sulfamethoxazole/trimethoprim. Thirteen out of fourteen strains were resistant to penicillin (A and C pulsotypes, A1 and C1 subtypes) and just one isolate was susceptible (B pulsotype) to all antibiotics tested. -
Analysis of longevity in Holstein Friesian cattle using proteomic approaches
21-25Views:634The aim of the present study was to determine marker proteins those are associated with functional longevity of dairy cattle. Holstein-Friesian cows were grouped based on their performance as follows: group 1) individuals with good longevity traits; group 2) short production life because of poor reproduction traits; group 3) short production life with low milk yield. Twelve individuals were sampled in each group, blood and milk samples were collected from cows. Blood samples were analysed with two dimensional polyacrylamide gel electrophoresis (2D PAGE), MALDI TOF/TOF and nanoLCMS/MS. The milk samples were analysed with MALDI TOF/TOF and nanoLC-MS/MS. Using the optimized gel based proteomic approach,
we have succesfully separated 143 proteins in the group1, 139 proteins in the group2 and 136 proteins in the group3, but we could not find significant differences between groups in the expression pattern. Using MALDI TOF/TOF and nanoLC-IonTrap MS, we have found eleven protein sequences those were expressed only in the samples of good longevity group.