No 23 (2006)

Published May 23, 2006

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Articles

Effect of year and irrigation on yield and quantity of different potato varieties
7-14

dramatically in the last few decades. We are lagging behind Western European countries as regards yields. The ecological conditions are not favourable for potato, but the low yields other causes: the use of non-suitable seed, the low level of irrigation and nutrient supply and out of date machinery. The competitiveness of production is further ...decreased by the great alternation in yields from year to year, unpredictable market conditions, poor consumption habit, and often the lack of quality products.
The experiment was carried out at the experimental site of the University of Debrecen, Farm and Regional Research Institute, at Látókép. In our experiment, we examined the yield and selected quality parameters of 9 medium-early varieties in large parcels. Of the examined varieties, 3 are of Dutch, and 6 are of Hungarian breeding.
The experiment was set up in 2004 and 2005, on 50 m2 parcels on calcareous chernozem soil after winter wheat (2004) and two rowed barley (2005) as a forecrop. The varieties were examined in 4 repetitions in randomized blocks, of which two repetitions were irrigated, and two were non-irrigated. We examined the yields of the varieties, the distribution of tubers according to size and their percentages and changes, and selected parameters of quality and chemical composition due to irrigation. We studied the dry matter content, the starch content, the underwater mass, the amount of reducing sugars, and the colour index of frying of the tubers.
Summary, it can be stated that among the agrotechniques, year effect, variety and irrigation factors have considerable impact on potato yield quality and quantity. However, the impact of irrigation depends on the year effect.

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Possibilities of selection and production of blue and ornamental corn (Zea mays L.)
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The cultivation of alternative crops has an important role in world agriculture. Their market share is continuously growing in the food industry sector. In the present study, we show the cultivation and breeding perspectives of ornamental and blue corn. There exists possibility to cultivate ornamental corn in Hungary. There are many cultivars o...f ornamental corn, with various kernel colors, husks, stalks and leave colors. Blue corn is unique among other corns. Blue corn higher in protein, iron and zinc than commercial dent corn. Pests affective ornamental and blue corn are the same as those of other commercial corns. Ornamental and blue corns need to be grown a minimum of 500 m away from commercial yellow dent corn to minimize any cross pollination which may result in off-color kernel. Our aim in the presentation of this review was to broaden Hungarian literature.

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Investigation of genetic diversity in irradiated maize lines and its relation to hybrid performance
20-26

Knowledge of genetic diversity among available parental lines is fundamental for successful hybrid maize breeding. The aims of this study were to estimate (1) genetic similarity (GS) and genetic distance (GD) (based on Jaccard index) in four maize inbreed lines; (2) to classify the lines according to their GD and GS; (3) to determine hybrid per...formance based on GD and heterosis for yield ability in 4x4 full diallel system. We used morphological description and AFLP (amplified fragment length polymorphisms) for estimation genetic polymorphism in four maize inbred lines. We estimated the applicability of genetic similarity in SC and reciproc hybrids for prediction of their performance.
Three primer combinations were used to obtain AFLP markers, producing 207 bands, 70 of whit were polimorphic. The dendogram based on genetic similarities (GS) and genetic distance (GD) and morphological description separated four inbred lines into well-defined groups. Morphological description just with AFLP analysis showed reliable results. In view of genetic distance, the UDL 1 line and their linear and reciprocal crosses showed significant heterosis effect, which was confirmed by heterosis calculation based on grain yield.

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The effect of season and fertilizer on the LAI, the photosynthesis and the yield of the maize hybrids with different genetic characteristics
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The experiment was carried out in Debrecen, at the Experimental Station of the University of Debrecen Centre of Agricultural Sciences, Department of Crop Production and Applied Ecology. We tested 10 various hybrids with their own genetic characteristics for five different fertilizer doses, in addition to the parcels without fertilization. The t...hree factors of production technology jointly determine the successfully of maize production, but in different measure. The yield and the stability of yield of maize can be increased with hybrid-specific technologies.
In 2004-2005 experiment years the favorable results reached were due to the rainy season. There were significant difference between the productivity of maize hybrids. The N 40, P2O5 25, K2O 30 kg/ha treatment caused the highest increase of yield (3-5 t/ha) compared to the control (parcels without fertilization). The reaction of hybrids to the further fertilizer doses was different. The agro-ecological optimum of NPK fertilization was N 120, P 75, K 90 kg of the most hybrids.
During the experiment, we tested the moisture loss of the five hybrids. The seed moisture content at harvest was higher than in previous years due to the rainy seasons. The seed moisture content of harvest of FAO 200-300 hybrids were about 20%. It changed between 21-24% in the case of hybrids with longer vegetation period (FAO 400), the seed moisture content of Mv Vilma (FAO 510) was 24.21-25.04% in the average of fertilizer treatments. There is an important difference between the moisture loss ability of hybrids which changed 0.2-0.6%/day. The moisture loss of hybrids changed depending on the fertilizer treatment; usually, it was more favorable in the optimal fertilizer dose (N120+PK).
In the case of tested hybrids, we measured the highest LAI and photosynthetic activity at the optimal treatment, N 120, P2O5 75, K2O 90 kg/ha in the respect of efficiency and environmental protection, and the yield was high also for this treatment. There are significant difference between the LAI, the photosynthetic activity and the yield of hybrids, and these values could change depending on the treatment of fertilization.

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The study of headspace sampling for the measurement of Volatile Organic Compounds byGC-MS
35-38

Headspace sampling was studied for GC-MS measurements. A quick, direct method was developed for the measurement of petrol, gasoline and volatile organic solvent contamination of soil. According to our results this method is suitable for quick qualitative analysis of VOC contamination of soil, and helps to make decisions about further measuremen...ts. We concluded that an accurate quantitative measurement is not possible.

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The effect of sowing time on the yield and the variance of the seed moisture content a harvest of maize (Zea mays L.) hybrids
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Sowing time is an important crop technology element of maize. We studied the effect of this factor on the growth and production of maize in an experiment carried out near Hajdúböszörmény, in 2003 and 2004, and near Debrecen, in 2005.
The soils of the experiments were humic gley soil and chernozem. Weather in both years differed greatly. ...2003 was drought. Neither the distribution, nor the quantity of the precipitation were suitable in the growing season for maize. This fact basically determined the results.
In 2004 and in 2005, there were favorable and rainy seasons. The distribution and quantity of precipitation were suitable between April and September. The average temperature was also suitable for maize.
In 2003, we tested seven hybrids at four sowing times. Hybrids with a shorter vegetation period gave the highest yield at the later sowing time, while the hybrids with a longer vegetation period gave them at the earlier sowing time. The yield of PR34B97, PR36N70, PR36M53 hybrids were the best at every sowing times. The moisture loss of hybrids in the late maturity group was faster in the maturity season, but the seed moisture content was higher than the hybrids with early sowing time. The seed moisture content was very low due to the droughty year. In two hybrid cases, this value was higher than 20% only at the fourth sowing time.
In 2004, we examined the yield and seed moisture contents of nine hybrids. In the favorable crop year, the yield of every hybrid was the highest at the second and third sowing times. Yields of PR34H31 and PR38B85 hybrids were significant. The seed moisture content at harvest was higher than the previous year, due to the rainy season. In the case of hybrids sown later, this value was higher by 30%. However, we noticed that this value was lower at the earlier sowing time, than at the later.
In 2005, we applied three sowing times. Unfortunately, the results of the third sowing time could not be analyzed, due to the low plant density. The yield of the six hybrids varied from 12 to 14 t/ha at the first sowing time. At the second sowing time, the yields fluctuated and each hybrid had the lowest yield, except the PR37D25 hybrid. At the latest sowing time, the yield of the PR34B97 hybrid was the lowest. However, this low yield was due to damage from the Western corn rootworm (Diabrotica virgifera) imago. The moisture content at harvest of the hybrids varied from 16 to 24% at the first sowing time. Yields at the second sowing time were higher. The low yield of the PR34B97 hybrid coupled with a higher seed moisture content. In addition, the maximum value of the LAI was more favourable at the first sowing time, and ranged between 5-5.5 m2/m2.
The crop year had a more dynamic effect on maize than the sowing time. First of all, the quantity and distribution of precipitation played an important role in respect to yield safety.

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The effect of the plant density for the yield of the maize hybrids
50-61

In order to enchance the yield stability of maize, the effect of plant density on yields was studied on a typical meadow soil in Hajdúböszörmény between 2002-2004. In the plant density experiment, we used the method of Béla Győrffy. The plant densities applied therefore 20 to 100 thousand plants/ha by ten thousand scale. The application o...f fertilizer rates for the maize hibrids in every year were N: 110 P: 90 K: 120 kg/ha. We used a manual soiling-gun in the experiment. In every year we used plant protection techniques against monocotyledonous and dicotyledonous weeds. The harvest was done by hand. The facts were read by variancie analysis and linear regression analysis. The moisture and the temperatures were extreme in 2002, 2003, 2004. We have to mention defficiery of moisture in 2003 which is shown that the hot days number increased. After evaluating our findings we can conclude that most hybrids showed a significant correlation between increased plant density and the volume of yields. On the basis of the experiments we divided the hybrids into four groups: the first group included the hybrids suitable for increased plant density with a wide range of optimal density values; the second group included hybrids, which did not require high plant density, were capable of good individual performance and tended to grow several ears; the third group included flexible corn types, which grew longer ears in favourable years, thus yielded more; and the fourth group included the hybrids, which were sensitive to increased plant density and which showed a narrow range of optimal density values. Finally, plant density determines the yield; we have to consider optimal plant density intervals as well as optimal plant density, and we also have to place a high emphasis on the use of hybrid-specific technologies.

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Quality comparison of selected apple cultivars under ULO-storage
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Our examination was carried out in the 103 ha apple orchard of the Kasz-Coop Ltd.. The plantation works with modern varieties standing on M9 root stock, planted in a tree density of 2500 tree/ha and treated with professional integrated technology. The date of harvest is defined by a combination of several ripening analyses methods. The orchard ...has its own ULO-system warehouse.
Used in the experiment were four apple varieties: ’Idared’, ’Golden Reinders’, ’Jonagold’ and ’Braeburn’.
While under ULO-storage, we took 20-25 piece samples 6 times regarding the four varieties, from which 5-5 pieces were kept under three weeks’ shelf life, measured on a weekly bases. In our research, we analysed the dynamics of the flesh firmness and the refractometric value under ULO-storage and three weeks’ shelf life, and the standard deviation dynamics of the refractometric value under ULO-storage.
Analyses were based on the following measurements: flesh firmness, refractometric value and number of mature seeds.
We looked for morphological and morphological-quality correlations.
Through these we described the dynamics of the flesh firmness and the refractometric value under ULO-storage and three weeks’ shelf life for each variety. We referred to the stability of these attributes and to the dynamics of their change. Later, with the help of these, and also considering our practical experience related to the measurements, we characterised the four varieties.
We found a positive correlation between the seed number and the flesh firmness in the case of the ‘Idared’, ‘Jonagold’ and ‘Braeburn’ varieties, but no correlation was found in the case of ‘Golden Reinders’ in this concern. We visualized the standard deviation dynamics of the refractometric values under ULO-storage. This allowed us to categorise the varieties.

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The effect of different herbicide on the number and activity of living microorganisms in soil
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Sustainable plant growth, considering the difficulties of weed elimination, cannot be effective without the application of herbicides. However, these chemicals have enormous ecological implications, including effects on the microbiological communities of soils. It is advisable to use herbicides that have minimal secondary effects on the environ...ment and soil-living microorganisms. In contrast, herbicides with prolonged growth stimulating or inhibiting effects are not suitable, because both types have strong influences on the number and activity of bacteria, thus causing changes in the ecological equilibrium.
Preceding small plot experiments, laboratory tests were carried out to study the effect of herbicides used in maize cultures on the number of bacteria and growth of microscopic fungi.
Substances that were observed to have stronger influences were applied in small plot experiments set up in the experimental garden of the Department of Plant Protection of the University of Debrecen. We studied the effects of four herbicides (Acenit A88EC, Frontier 900 EC, Merlin SC and Wing EC) on the microbiological properties of the soil. These herbicides were used in different concentrations in maize culture, and we investigated the effects in different soil layers.
In the laboratory experiments, we determined the total number of bacteria and microscopic fungi and examined the growth of Aspergillus niger, Trichoderma sp. and Fusarium oxysporum on peptone-glucose agar containing herbicides.
During the small plot experiments, soil samples were collected 3 times a year from 2-20 cm depth. The total numbers of bacteria and microscopic fungi were determined by plate dilution method, while the method of most probable number (Pochon method) was used to determine the numbers of nitrifying bacteria and cellulose decomposing bacteria. To evaluate the microbiological activity of the soil samples we measured carbon-dioxide release (after 10 days incubation), nitrate production (after 14 days incubation) and the concentration of C and N in the biomass.
We can summarize our results as follows:
• In laboratory experiments, herbicides caused a decrease in the number of bacteria and inhibited the growth of microscopic fungi.
• Frontier 900 EC and Acenit A 880 EC had the strongest inhibiting effect on microorganisms.
• In small plot experiments, herbicide treatment decreased the total number of bacteria and microscopic fungi.
• Herbicides caused a significant increase in the number of nitrifying and cellulose decomposing bacteria.
• Different herbicides containing the same active compound had similar influences on soil microoorganisms.
• A significant increase was observed in the physiological processes of tolerant microorganisms surviving the effects of herbicides

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Effect of weather conditions on the protein content and baking value of winter wheat flour
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We searched for connections between weather conditions (with its sub-parameters as precipitation and average temperature) and the yearly formation of two quality parameters (protein content and baking value) on three levels of mineral fertilization, based on the results of a variety comparison experiment on chernozem soil, to select those weath...er parameters and critical periods which have significant effects on the quality of winter wheat flour.
We established that the protein content of winter wheat flour can be increased with increasing levels of mineral fertilizers. Protein content is lower and has higher deviation during non-fertilized conditions in different cropping years than on higher fertilization levels. Thus, it seems proved again that quality (as protein content) is mainly formed by the crop year, but can be improved with adequate agricultural engineering (with mineral fertilization in the present case). The higher sum of precipitation in May, and the lower average temperature after flowering, have the highest increasing effect on the protein content of flour of the examined parameters. Based on the results of the examined period, the rainier and warmer term than average before flowering and lower – average amount of precipitation and colder circumstances are favourable for higher baking values. The analysis with data of decades, proves the importance of the first half of May and the middle of June as especially important periods for quality formation. An increasing nutrient supply has different effects on the varieties; mineral fertilization increased the baking value of GK Öthalom winter wheat variety in almost every case, but the second level of fertilization decreased it in half of the examined years. Additionally, mineral fertilization played a role in the stabilization of the quality of highlighted varieties.

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Impact possibility of hedgerows on plant protection and on the yield of winter wheat
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Agricultural hedgerows have various impacts on the surrounding cultivated area and on the landscape. They serve as a windbreak, help manage humidity and precipitation, and are also sources of plant species richness which can contribute to the animal biodiversity. One of their beneficial effects is the yield improvement of agricultural crops. Ou...r investigation tries to prove this hypothesis in Hungarian circumstances in the case of several winter wheat fields near Debrecen. Regarding the results, it seems that the majority of data supports the relevant, mainly German and Austrian information, but in some cases, the correlation has not been significant.

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Young-making of sour cherry cultivars and examination of their regenerative characteristics
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Although a high portion of domestic orchards is cultivated extensively, quality Hungarian sour cherry is a highly demanded product throughout Europe. Trees are slightly pruned, or not pruned at all. Thanks to the unique character of the species, older wood parts are pilling up. Thus, the interior of the canopy is inactive, fruits and shoots con...fine the exterior layer of the canopy.
We established pruning treatments in the sour cherry plantation in the Horticultural Exhibition Garden of the University of Debrecen, involving two age groups in two spacings. The trees are standing on seedling rootstock. The involved varieties were: Érdi bőtermő, Debreceni bőtermő and Kántorjánosi. We examined how to encourage shoot formation and the regeneration of older, pilling wood of the interior canopy parts.
Applying strong rejuvenating cuts, the most shoots were generated on the 3rd and 4th year laterals. Wood parts older than the 3rd year shall be removed by leaving short (2-3cm) stubs. This way, older, passive woodparts can also be made to burst out. From the examined varieties the Kántorjánosi showed the most favourable regenerative ability. Due to summer pruning, more shoots regenerated on the axis of the trees, than on those rejuvenated in the dormant season.

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Selenium-speciation experiments from soil samples by accept of ionchromatography-inductively coupled plasma mass spectrometry (IC-ICP-MS) method
106-111

In these days, selenium is one of the most investigated microelements. It has an important proven role in many vital processes. Selenium deficiency can play a role in the contraction of many diseases (e.g. cancer, heart diseases, etc.) and in the aggravation of their clinical aspect directly or indirectly. In this paper, we study the soil sampl...es of an outdoor experiment for the conversion of the inorganic selenium salt that was spread out in different doses. The danger of the wash-out effect was also investigated. Our experiments showed, that most of the inorganic selenite transforms to selenate form during the years, and in this selenate form it moves to the deeper layers of soil.

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Application of the SDS-PAGE method for the characterisation of winter wheat flour quality
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The principle, development and importance of the SDS-PAGE method are presented in this article. The SDS-PAGE method has become one of the basic methods of molecular biological research, because it is widely applicable and its sensitivity is excellent in the separation of wheat storage proteins.
We have shown the application of this method wi...th a concrete example. It was also tested whether, it was possible to obtain a better baking quality product from a large amount of poor quality less valuable wheat by fractioning the flour according to particle sizes after grinding. We studied the rheological properties of flours with different particle size fractions from the original flour. The baking quality of the original flour was B2. The 125-90 and 90-63 μm fractions have significantly better baking quality (B1) than the original flour. The protein contents of these flour fractions were also significantly higher than the protein content of the original flour. We had a question: what has influenced the baking quality: the protein content or other factors? We searched for an explanation on these results in the protein composition of the flour samples. We studied the distribution of glutenin-fractions by SDS-PAGE method and evaluated them. We found with correlation analysis that the amount of LMW-Glutenin D-group (52-60 kDa) is in a strong, negative correlation to the baking quality (r = – 0.855*). Therefore, the baking quality of flour samples was influenced by this glutenin fraction.

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Influence of Foliar Treatments on the yield and quality of Sugar Beet
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The yield and quality of the sugar beet are mainly determined by the plant production system, thus we studied the effect of mineral fertilization. Our field trials were carried out in 2005 in Hajdúböszörmény, at two sites. We studied the effect of sulphur (Cosavet DF), copper (Kelcare Cu) and two foliar nutrients with high active agents. Tr...eatments were replicated four times.
We took root samples at 4 week intervals, starting in August. The quality of root (sucrose, potassium, sodium and alfa-amino N content) was determined from filtrated beet broth, by an automatic beet laboratory system, called VENEMA.
We found that the crop and the sugar yield were significantly influenced by the foliar treatments

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The composition of gluten proteins and their effect on the rheological properties of gluten
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Wheat is the major cereal component of bread in the world and is grown worldwide. Of the cereals only the bread wheats – and less the triticale – includes storage proteins that play an important role in the performance of gluten. Proteins of gluten complex may be present in two classes:
− low molecular weight (gliadin-) components, and...
− high molecular weight (glutenin-) components.
Gliadins shown appreciable heterogenity and can be separated into 40-50 components with gel electrophoresis. The composition of gliadins is employable for the identification the wheat varieties and to investigate the varieties. In the decreasing electrophoretic mobility sequence may be distinguish α-, β-, γ- and ω-gliadins. A glutenin subunits may be include in two classes:
− high molecular weight glutenin subunits (HMW-GS),
− low molecular weight glutenin subunits (LMW-GS).
Wheat varieties can be identified by glutenin and their quality selection is also possible. The gliadin’s polypeptides encoding genes are located on the short arm of chromosomes 1A, 1B and 1D, 6A, 6B and 6D. Genetic coding for HMW subunits is located on the long arms of chromosomes 1A, 1B and 1D, the LMW-GS are also located on chromosomes 1A, 1B and 1D (Glu-3 loci) near the gliadin-coding loci.
Storage proteins affect the rheological properties of gluten by two factors:
1. The quality and quantity of the protein components of the gluten complex,
2. The interactions between the protein fractions.

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