No 70 (2016)

Published October 24, 2016



The analysis of flour blends as affected by the behaviour of two different quality flours of triticale under different fertilizer treatments

Triticale is likely used in many countries in human consumption, due to its advantageous agronomical and nutritional properties mostly in blends. The baking quality of blends depends not just on the proportions of the used flours but also on their individual quality what can be influenced by fertilizer treatments.

22 flour blends were p...repared with commercial wheat flour and triticale wholemeal flour in proportions from 0% to 100%. The triticale was treated with different amount of fertilizers (N30P30K30, N60P0K0). Changes of wet gluten contents and extensograph parameters of the blends were determined. The quality of blends significantly depends on the fertilizer treatment of triticale beside the proportions of the flours. When the N60P0K0 treated triticale was used in blends, wet gluten and extensibility values were significantly higher, but in case of resistant to extensions (Rmax) the N30P30K30 treated samples gave higher values. The measured values of wet gluten and extensibility were above the expected values (synergism), while in the case of resistance to extension the expected values were higher than the measured values (antagonism).

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Growth regulators influence on stability of shoots and ascorbic acid content at cadmium and nickel joint action

It is shown that for a maize the most effective protector of cadmium and nickel influence was zeastimulin, for a pea – agrostimulin.A protective function of emistim C for both cultures was insignificant. It is set that zeastimulin is influential in the increased metals absorption of a root system, however substantially (on 15% for a nickel an...d twice for a cadmium) reduces their translocation to above-ground part of plants. Previous treatment of pea seed by agristimulin also intensified the accumulation of nickel roots on 60% and did not influence on cadmium absorption and toxicants translocation to tissues of assimilatory organs. The protector role of growth regulators to cadmium and nickel joint influence shows up in the increase of ascorbic acid maintenance in the roots cells of both species to 34%, where as in leaves – at a maize resulted in the lowering of vitamin С amount (on 28%), but at a pea – increase on 20%.

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Examination of the interval between litters (IBL) of different genotype HLW sows using survival analysis

In this study our aim was to find out if there is a difference between the genotypes determined for the previously identified mutations of seven genes of the Hungarian Large White in terms of the time spent in production. We identified the previously determined alleles of the seven genes (BF, EGF, ESR, FSHβ, H2AFZ, LEP, PRLR) related to prolif...eration that were and performed the survival analysis between breeds indicating the risk of culling and the time spent in production on the given farm. Based on the results of survival analysis by Log-rank test, Breslow (Generalized Wilcoxon) and Tarone-Ware test we concluded that they indicated a significant difference in case of the genes BF (Breslow and Tarone-Ware tests) the EGF (Log-rank and Tarone-Ware tests) and ESR (Log rank test) based on which the curves of the survival of the certain genes varied form one another significantly.

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Chromatometric comparison of Eurasian Collared Dove (Streptopelia decaocto) and Feral Pigeon (Columba livia domestica) feathers

Chromatometric examination of the plumage of birds is a poorly researched topic. We have approached this issue in primarily aspect of differences in plumage of species. Moulted feathers sample collection method has been increasingly used. Reliable identification of feathers becomes an increasingly important issue, hence need for an exact measur...ement-based methodology. Eurasian Collared Dove (Streptopelia decaocto) and Feral Pigeon (Columba livia domestica) primary, secondary and tail feathers were studied. Chromatometric parameters of feathers were measured in CIELAB color system and then statistical analysis (Independent samples t-test, Descriptive Statistics, Discriminant Analysis) was performed to compare the two species. Instrumental measurements has been confirmed the high similarity between colors of the two species, however species specific differences were also found. Lightless (L*) value were significantly characteristic of particular species, while the red/green (a*) and yellow/blue (b*) value had lower Predictive Power. We identified feathers and the variables which useable to separate the two species and determined the associated Confidence Intervals of these values. Our results may draw attention to a new potential direction for exact identification of the moulted feathers during sample collection.

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Influences of different organic fertilizers on nutrients of humic sandy soil and on the growth of Spinach (Spinacia oleracea L.)

A greenhouse pot experiment was conducted to compare the effects of manure with different origin (horse, cattle), various bedding materials (straw, sawdust) and diverse doses (30 t ha-1, 60 t ha-1) and the impact of food waste compost on the plant growth and the available plant nutrient content of soil. The study was condu...cted on humic sandy soil and consisted of 9 treatments in a randomized complete block design with four replications. Spinach (Spinacia oleracea L.) was grown as the test crop. The treatments were: 1. unfertilized control; 2. horse manure with straw (30 t ha-1); 3. horse manure with sawdust (30 t ha-1); 4. cattle manure (30 t ha-1); 5 food waste compost (30 t ha-1); 6. horse manure with straw (60 t ha-1); 7. horse manure with sawdust (60 t ha-1); 8. cattle manure (60 t ha-1); 9. food waste compost (60 t ha-1). Plant growth was monitored for 4 weeks. Shoot and root weights per pot were measured, total biomass weight per pot were counted.

On the basis of the results it can be concluded, that among treatments the application of horse manure with straw enhanced spinach growth most significantly compared to other treatments and to the non-treated control, resulted the highest weights of leaves and roots of spinach. At the same time even small dose (30 t ha-1) of this fertilizer caused increased plant available nitrogen and phosphorus of soil and the higher dosage further increased these values. The horse manure with sawdust applied in lower dose did not alter the leaves and roots weights, but higher portion (60 t ha-1) caused significantly decreased plant biomass. The results proved that the bedding material may significantly alter the composition of manure and may change the plant nutrition effect of organic fertilizer. Cattle manure and food waste compost in both applied doses enhanced plant growth. Both fertilizers increased the plant available nitrogen forms and phosphorus content of soil, but cattle manure caused higher increase.

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Genetic diversity of the Hungarian draft horse assessed by mitochondrial DNA

Hungarian draft is a horse breed with a recent mixed ancestry. It was developed in the 1920s by crossing local mares with draught horses imported from France and Belgium. To genetically characterize the breed and to set up the basis for a conservation programme, we have employed a molecular marker: a 256-bp D-loop mitochondrial DNA fragment. We... analyzed 124 horses representing Hungarian draft horses to assess the maternal phylogeography of the breed. Sequence analysis of a 256-bp segment revealed a total of 34 haplotypes with thirty-four polymorphic sites. High haplotype and nucleotide diversity values (Hd=0.953±0.001; π=0.024±0.001) were detected. The average number of pairwise differences were k=5.998. This breed counts 800 mares today, and only survive due to breeding programmes, this way each haplotype frequency depends on the extent to which mares are involved into the breeding. The reduced number of surviving maternal lineages emphasizes the importance of establishing a conservation plan for this endangered breed. Due to the revealed 34 polymorphic sites we could presuppose twelve maternal linages, which could be a first step for making a breeding programme.

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Evaluation some important microbiological parameters of the carbon cycle in chernozem soils profiles

Some chemical and microbiological properties of the carbon cycle were investigated in three chernozem soil profiles. The soil profiles originated from a long term fertilization experiment (potato) of the University of Debrecen, Látókép, Kryvyi Rig Botanic Garden (grassland) and a large-scale farm (sunflower) of Ukraine. The results of the or...ganic C-content, total number of bacteria, microscopical fungi, cellulose decomposing bacteria, CO2-production, microbial biomass carbon and saccharase and dehydrogenase activities were compared and evaluated with the help of correlation analyses. Close correlation was found between the organic carbon content and the number of microscopical fungi,, saccharase and dehydrogenase enzymes’ activities, as well as close correlation was found between the dehydrogenase activity and microbial biomass-C and saccharase activity.

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Impact of weather on the spring crops yield in Croatia with emphasis on climatic change and the 2014 growing season

Main field crops in Croatia are maize, soybean, sunflower and sugar beet. By these crops are covered (status 2014) close to 50% (385 234 ha) of utilized arable land. Global warming, have often adverse influence on field crop yields. Aim of this study was testing precipitation and temperature regimes on spring crops yield in Croatia in 15-year p...eriod (1999–2013) and elaboration of the 2014 growing season with aspect of climatic change.

Four growing seasons (2000, 2003, 2007 and 2012) were less favorable for maize because annual yield was bellow 5 t ha-1 (average 4.38 t ha-1), while in four more favorable years (2005, 2008, 2009 and 2010) annual yield was above 6.8 t ha-1 (average 7.32 t ha-1). Average precipitation and temperature for the April-September period in Osijek were 226 mm and 496 mm, 19.6 oC and 18.6 oC, for less and more favorable years, respectively. Yields of soybeans and sugar beet have mainly similar trend as maize yields in function of weather conditions, while sunflower is more susceptible to extremely moist growing seasons (for example, 2001 and 2005: 650 mm and 697 mm precipitation and very low yields in level 1.7 and 1.6 t ha-1, respectively). On the other side, under drought conditions of 2003, 2007 and 2012, yields of sunflower were above average in range from 2.5 to 2.7 t ha-1), while at same period yields of maize, soybean and sugar beet were drastically reduced.

Average precipitation in the April-September period of 2014 for eight selected sites of Croatia was 756 mm or for 68% higher in comparison with the long-term average 1961–1990 with variation among the sites from 520 mm in Osijek to 910 mm in Varazdin. On the other side, average air-temperature in 2014 was 17.8 oC or for 0.7 oC higher with variations among the sites from 17.2 oC in Daruvar and Varazdin to 18.2 oC in Osijek and 18.3 oC in Gradiste. Under these favorable weather conditions, annual yields of maize (8.1 t ha-1), soybeans (2.8 t ha-1) , sunflower (2.9 t ha-1) and sugar beet 63.6 t ha-1) were considerable higher than usual.

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The study of the fertilizing effect of wheat straw ash in a greenhouse experiment

The effect of wheat straw ash as a fertlizizer was studied in a pot experiment with an acidic sandy loam soil (pHKCl=4.9) with weak K and P supply. The test plant was ryegrass (Lolium perenne). The treatments were the following: 1. control untreated soil, 2. NPK fertilizer, 3. small dose of ash (1.4 g kg-1), 4 large dose o...f ash (2.8 g kg-1), 5. small dose ash completed with NP fertilizers. Soil parameters (pHH2O, pHKCl, ammoinum-lactate soluble P, K, 0.01 M CaCl2 soluble PO43--P, K, Mn, Cu, Zn ) and plant parameters (yield, P, K, Ca, Mg, Zn, Mn uptake) were investigated. Based on the analysis of the straw ash sample and the results of pot experiment it can be stated that the wheat straw ash is suitable for the fertilization of the studied soil. The small dose ash completed with NP resulted in the largest yield increment (43%). In order of the treatments the pHKCl changes to: 4.9, 4.8, 5.2, 5.8, 5.1. As the N : P2O5 : K2O ratio is 0 : 1: 3.5 in the wheat straw ash sample, to reach optimal yield ash should be completed with N and P.

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Features of forming an invertebrate fauna in technogen environment (Kriviy Rig, Ukraine)

The features of invertebrate fauna forming in conditions of technogen pressure (Kriviy Rig, Ukraine) were studied. The taxonomic composition, dominance structure and correlation of soil invertebrates’ functional groups in the large industrial enterprises were examined. It had been found that a small thickness of the soil layer as a habitat fo...r ground animals causes the depletion of taxonomic composition, species richness and decrease the total number of soil invertebrates. Gastropoda molluscs in conditions of technogen pressure are the most adapted to stresses group of invertebrates.

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The marigold (Calendula officinalis L.) drug essential oil agents change under different fertilization settings in small plot trial

During our research we investigated the marigold's (Calendula officinalis L.) nutrient requirements with different fertilization setting in small-plot trial. We measured SPME (Solid phase microextraction) and GC-MS (gas chromatograph-mass spectrometer) we examined the effects of the different fertilization settings for the herb's main active in...gredients of essential oil's percentage.

Based on the results, it was concluded, the essential oil agents' percentage breakdowns significantly depending on the cropping technologies. Besides that it is possible, based on Pearson's correlation test the marigold essential oil agents relationship can also be a major factor.

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Effects of paraffine oil on leaf and berry mycobiota on two grape varieties

Application of fungicides have advantages and also some direct or indirect disadvantages, such as imbalance and/or fungicide resistance in microbe population. To avoid these problems the development of alternative, eco-friendly methods like mostly spraying with oils are in the focus nowadays. The investigations of the effects of fungicides on m...icrobiota in some cultivations can give a more complex view to this topic and developmental possibilities. In the present study, our aim was testing of the effects of paraffine oil (as alternative fungicide) on microbial properties (CFU and rate of filamentous fungi and yeasts) of Chardonnay and Kékfrankos leaves and berries.

Our results from 2014 showed that the application of paraffine oil as sole spray agent can decrease the presence of saprophytic filamentous fungi on the berries of Chardonnay (susceptible for fungal infections). In the case of Kékfrankos berries opposite properties were observed, which may be the result of the absorption of oil by the thick wax layer of this variety. The oil treatment did not affect the yeast population of Chardonnay and Kékfrankos berries contrary to negative effect of the regular pesticide treatment. The selective fungicide effect of paraffine oil against filamentous fungal population caused the accumulation of yeast cells in the mycobiota of grape berries. The careful use of this yeasts in spontaneous fermentation can improve the aroma profile of wines. The year of 2015 did no prefer the growth of fungi, therefore no interesting properties were detected in the mycobiota of grape varieties. The occurence of the harmful saprophytic filamentous fungi predicted to be increased in mild climate agricultures as the result of the climate change.

In summary, the paraffine oils are seem to be promising tools for the eco-friendly control of harmful fungi of grapes.

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The dynamics of biodiversity structure of soil microorganisms under the impact of biopreparations during potato growing season

The use of biological preparations such as Phytotsid and Planryz contributes the increase of the general number of soil bacteria population by 13.0–36.1% in the case of potato variety Scarbnytsya and by 4.5–24.6% for potato variety Oberig compared with control. It also increases the number of saprophyte microflora, which compete with plant ...pathogens, micromycetes, and causes 1.2–1.8 times reduction in the number of soil fungi – Fusarium and Alternaria. During the application of Rovral Akvaflo the Shannon ecological index of species biodiversity is lower than during the biopreparation use. The decrease of species biodiversity was observed as well as strengthening the dominance of some species (dark pigmentation in fungi).

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Survival analysis of sow longevity and lifetime reproductive performance – Review

Sow longevity plays an important role in economically efficient piglet production. Improving sow longevity results increase in the productivity and profitability of a sow herd. Longevity is a complex trait with many factors that can contribute to a sow having a long and productive life. Not only the sow’s genetics, but also nutrition, environ...ment and the management policies are important. In addition, the removal of non-productive sows along with the introduction of replacement gilts is an essential part of maintaining herd productivity at a constantly high level. The objective of this paper was to summarize the current knowledge about the sow longevity and lifetime performance values, as well as, discusses the survival analysis methods for sow longevity traits. This method can estimate the hazard rate indicating proportional risk of sows being culled at any given time, in addition, it is able to investigate the effects of different factors on longevity. Identifying factors that influence the longevity could assist many commercial pig producers in becoming more efficient.

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Lack of polymorphism of the agouti signaling protein (ASIP) gene among four different brown hare (Lepus europaeus Pallas 1778) populations

The brown hare (Lepus europaeus Pallas 1778) is a common palearctic and a popular game species therefore it has been an obvious subject for population genetic studies since the second part of the 20th century. Among the several mitochondrial DNA studies some have been carried out concerning nuclear genes as well. The agouti signaling... protein gene (ASIP) is involved in regulating the synthesis of eumelanin and pheomelanin in melanocytes of mammals. Though many studies focused on it in relation with several mammalian species, minimal information is available on this topic concerning the brown hare.

Here we present a short communication concerning the agouti signaling protein (ASIP) gene in four different country’s L. europaeus populations, namely Lithuania, Hungary, Serbia and Georgia. N=45 tissue samples have been investigated from overall 17 sampling sites of the different countries. There has not been found any polymorphism among the sequences. In an alignment with other Leporid species’ partial ASIP sequences downloaded from ENA we have found that based on a 178 base pairs long DNA sequence the haplotype of our samples contains three other Lepus species as well. This is concordant with the findings of a previous study focusing predominantly on the European rabbit (Orycto lagus cuniculus Linnaeus 1758) and the several mutations of its ASIP gene.

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Effect of N, P and K fertilisers and their interactions in a long-term experiment on winter barley (Hordeum vulgare L.)

The aim of this work was to analyse the effect of K, P and N supplies on the yield of winter barley in a long-term mineral fertilisation experiment with clearly distinct soil nutrient supply levels in order to develop fertilisation guidelines for winter barley growers. The experiment was set up in 1989 on a chernozem meadow soil calcareous in t...he deeper layers, applying all possible combinations of 4 levels each of N, P and K fertiliser, giving a total of 64 treatments.

The results of analyses performed in 2011 and 2012 can be summarised as follows:

  1. In 2011, when rainfall supplies were deficient in the shooting phase, improved K supplies (324 mg kg-1 AL-K2O) increased the grain yield, but in 2012, when rainfall supplies were more evenly distributed, K supply levels in the range 210–335 mg kg-1 AL-K2O had no significant influence on the yield of winter barley.
  2. An analysis of the P treatments revealed that, compared to the 119–133 mg kg-1 AL-P2O5 level (P0), better P supplies (186–251 mg kg-1) led to a significant increase in the grain yield.
  3. In both years rising N rates significantly increased the yield up to an annual N rate of 160 kg ha-1.

      4. A K×N interaction could only be detected in the nutrient supplies of winter barley in 2011. The yield-increasing effect of N fertiliser was more    pronounced at better K supply levels, while K fertiliser led to higher yields in the case of better N supplies.

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Fusarium culmorum isolated from rhizosphere of wooly cupgrass (Eriochloa villosa) in Debrecen (East Hungary)

Wooly cupgrass (Eriochloa villosa) is an East-Asian originated weed species and it has been spreaded worldwide by now. The first occurrence of this species in Hungary was observed and published in 2008 nearby Gesztely village (Borsod-Abaúj-Zemplén county, North-East Hungary) than in the summer of 2011 a significant population was discovered n...ext to Debrecen city (Hajdú-Bihar county, East Hungary).

In 2013 this weed was also reported from Szentborbás village, Somogy county (South-West Hungary). These observations of spreading and its biological features (production of stolons and large number of seeds, moreover herbicide tolerance) indicate that wooly cupgrass (E. villosa) has a great potential of invasiveness, so it may become a hazardous weed not only in Hungary but in all over the world.

The objective of this study was to identify the fungus which was isolated from wooly cupgrass (E. villosa) root residue samples which were collected after maize harvesting on arable land in late autumn, near Debrecen. The identification of the fungus based on morphological characters of colonies and the features of conidia developed on potato dextrose agar (PDA) plates. After the examination of axenic culture we revealed that the fungus from rhizosphere of wooly cupgrass was Fusarium culmorum. Pathogenicity and/or endophytic relationship between the fungus and wooly cupgrass is still uncertain so pathogenicity tests and reisolations from plants are in progress.

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