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• Effect of basal and foliar application of boron and zinc on yield attributes in Solanum melongena L.

  97-99.

  S. V. S. Chauhan

  A. Rana

  S. Chauhan

  Views:

  286

  Field experiments were conducted during 1999-2000 and 2000-2001 to study the effect of basal application (BA) of zinc sulphate (5 and 10 mg kg^-i soil) or boric acid (1 and 2 mg kg^-1 soil) alone or foliar applications (FA) of water or 1% ZnSO₄ or 1% H₃ B0₃ or the combinations of both BA and FA of either ZnSO₄ or H₃ B0₃ on yield attributes (number of flowers and fruits/ plant, fruit weight, size and total yield). On the basis of the two-year data, it was found that all the treatments of BA as well as FA enhanced yield attributes but the combination of BA of 10 mg kg^--1 soil ZnSO₄ and FA of 1% H₃ B0₃ gave highest yield as compared to other treatments and control plants.

• Studies on the alkaloid production of genetically transformed and non-transformed cultures of Lobelia inflata L.

  65-71.

  E. Szőke

  I. Bálványos

  L. Kursinszki

  A. Krajewska

  A. Mészáros

  A. Neszmélyi

  Views:

  304

  The investigations of the growth and alkaloid production of cell suspension-, callus-, organized- and hairy root cultures from Lobelia inflata L. proved that these cultures are able to synthesize the characteristic piperidine alkaloids of the intact plant. Alkaloid precursor amino acids (Phe, Lys) and plant growth regulators affect not only the growth and differentiation of tissue cultures but also their secondary metabolism. The synthetic regulator Sz/I I combined with Phe increased the total alkaloid content considerably in callus- and organized cultures; regulator Sz/28 especially increased the lobeline content (in organized cultures in response to Lys, in callus tissues as a result of Phe application). With the aim of optimizing growth and alkaloid production of the genetically transformed hairy root cultures of Lobelia inflata L. we studied the effect of some growth regulators (NAA, IAA, kinetin) and precursor amino acids (Lys, Phe). The kinetin had inhibiting effect on the growth and lobeline production of the hairy roots. The IAA and NAA increased the biomass formation and lobeline production. The highest lobeline level was detected in tissues cultivated on hormone-free medium containing Phe.