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Physiological and biochemical evolution of peach leaf buds during dormancy course under two contrasted temperature patterns
15-19.Views:114Budbreak anomalies in temperate fruit trees grown under mild conditions have often been described. However, only few authors approached the physiological evolution of leaf buds all along the dormancy period according to the temperature pattern. The aim of this study was to characterize the evolution of peach leaf bud dormancy through some physiological and biochemical parameters under temperate winter conditions and under total cold deprivation after the endodormancy onset. Two treatments were applied in peach trees cv. Redhaven: (i) Regular Chilling Amounts — RCA and (ii) Total Chilling Deprivation — TCD. Buds were sampled periodically from different parts of the stem (terminal, medium and basal ones). We recorded the evolution of: carbohydrate concentrations (glucose, fructose, sucrose, sorbitol and starch), respiration rate, water contents and energy metabolism (ATP and ADP ratio). The dynamics of these parameters were compared and correlated with dormancy evolution ("one node cuttings" test) and budbreak patterns in plank:. The endodormancy intensity of terminal buds was significantly lower than those of median and basal buds in early October. Under RCA treatment, this gradient faded and the bud endodormancy release was completed at the same time in all positions along the stem. Thereafter, the "cuttings" test indicated that terminal buds grew slightly faster than median and basal buds, and, consistently, budbreak in planta started with the terminals buds, followed by the medians and then by the basal ones. The carbohydrate contents showed a transitory change only when the buds began to grow after the endodormancy was released under RCA. Respiration, water content and ATP/ADP changed dynamics only under RCA and only after the end of the endodormancy (their respective changes were very parallel). The dynamics of none of the tested parameters could be related with the endodormancy dynamics, but respiration, water content and ATP/ADP could be consistent markers of the actual bud growth before bud break (in this respect, ATP/ADP could not show differences between the terminal and axillary buds while respiration and water content could).
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Sampling experience in a cherry plantation
21-28.Views:196Experiments in a cherry plantation were performed to find out that samples taken from various parts of the foliage of the tree what extent represent the whole tree. One tree from three different cherry varieties was selected. In the selection, we tried to form a good representation of the foliage of the orchard. 8 sampling locations were designated on each tree, in two different heights and four different directions according to the points of the compass.We measured the main sizes of the fruits in three orthogonal dimensions with a digital slide gauge with an accuracy of 0.01 mm; the mass of each cherry by an analytical balance, with an accuracy of 0.001 g; the mass of the stones with the same analytical balance, and then calculated the stone-pulp ratio. The measured and calculated data are used to characterize the sample in question.
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Fruit Quality of Sweet Cherry Cultivars Grafted on Four Different Rootstocks
59-62.Views:175A rootstock trial planted at the Szigetcsep experimental station in 1989 involved the study of two cultivars- `Germersdorfi FL 45' and 'Van' -grafted on four different rootstocks — 'Colt', `MxM 14 — Brokforest', `MxM 97 — Brokgrow' and 'Saint Lucie 64' as a control. The trees were trained to the "Modified Brunner-spindle" system and came into bearing in 1993. The yield per tree, fruit weight and fruit diameter have been measured each year since then. The refractivity, the acid content of the fruit juice, fruit cracking after four hours' dipping in water and stone weight ratio have also been measured since 1995. In 1997 and 1998 these parameters as well as fruit cracking after 24 hours' dipping were measured. Fruit firmness and fruit colour were also estimated. In almost every observed parameter significant differences were found between the scion cultivars. Yield efficiency was significantly higher on 'NUM 14' and 'Saint Lucie 64' than on the other two rootstocks. As regards fruit weight, in both cultivars and over an average of six years, it was found that trees on low yielding tree on 'Colt' rootstock had the highest fruit weight values and on heavy producing `MxM 14' the smallest. Soluble solids content was higher on 'Colt' and `MxM 97'. No significant differences between the rootstocks were found in acid content of the fruit juice. There were significant differences between the rootstocks in fruit cracking after 4 and 24 hours' dipping in water. Seemingly with respect to cultivars and rootstocks the year has a considerable effect on fruit cracking.
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Abnormalities of the stigma of sour cherry cultivar
31-33.Views:233The objective of this study was to evaluate the ratio of blackness of the surface of stigma of sour cherry cultivars. At the full bloom time of sour cherry 100 new opened flowers were marked in the internal (Inside), external (outside), bottom and upper parts of the crown of each cultivars including sour cherry cultivars ‘Érdi bőtermő', `Debreceni bőtermő', `Kántorjánosi', 'R. clone', 'Petri', Pándy', and 'D. clone'. The trees were replicated four times. The numbers of flowers with black stigma were counted and the percentage of dead stigma was calculated. In addition, tissues of black stigmata were investigated for blossom pathogens by microscopy. After flowering time the fruit set of the marked flowers counted and then percentage fruit set was calculated. Numbers of counted flowers were between 300 and 980 depending on the four position of the tree. Black color of stigma could be seen only on three cultivars (`Debreceni bőtermő', Érdi bőtermő' and 'Petri') out of seven assessed cultivars. The highest numbers of black colored stigma were found on cultivar ‘Érdi bőtermő' which ranged between incidences of 12 and 21%. Black stigma was never able to produce a fruit set. Microscopic examination revealed no pathogens associated with black stigma. Different part of the tree resulted different amount of black stigma. Black stigma was the largest on the outer part of the tree on cv. 'Érdi bőtermő' but also bottom part of the tree also produced larger number of black stigma on cvs. `Debreceni bőtermő' and ‘Érdi bőtermő'. Though symptoms were not typical to frost damage, we believe that black stigma is probably due to environmental factors during flowering. This might be associated with late spring cold coming from the soil surface as the bottom and outer part of the tree was more suffered from the disease.
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Pollen morphology of fruit species
49-57.Views:1037Size and surface morphology of pollen has been studied in 87 twit varieties of 10 fruit species during the period of 1990-1995. No preceding work of that type came to our knowledge, yet.
The samples comprised a wide variety of cultivars included male sterile, self-incompatible, partially self-fertile stone fruits, diploid and hexaploid plums, diploid and triploid apples.
The large number of species and varieties facilitated the comparison of items within and between the respective species.
It was stated that the size, shape and surface morphology of pollen is genetically determined and those data, combined with other variety characters, are suitable for the classification and distinction of varieties.
In assessment of pollen size and shape, their moisture content is crucial. The major diameter of the swollen pollen as well as the length and width of the dry grains are characteristic to species and/or to variety.
The width and shape changes largely with moisture content. Large grains are proper to quince, apricot, peach and almond, medium sizes are found in apple, sweet cherry, sour cherry, European plum, whereas small size is typical to Japanese plums.
The low number of varieties studied does not allow conclusions concerning differences within pears, quinces and almonds as species. In the rest of species, valid differences have been registered as between varieties.
Within species, as apple and plum, the effect of ploidy (i.e. number of chromosomes) was expressed in the size of their pollen. In stone fruit species, the correlation between size. of anthers and size of pollen grains was positive.
Genetic relations between the self-fertile sour cherry varieties of the Pándy type (Debreceni bőtermő, Kántorjánosi, Újfehértói fürtös) as well as the self-incompatible apricots of "giant" fruit size are supposed to be analysed by pollen studies but there did not turn out any decisive conclusion, yet. Other characters also should be considered.
The assembly of pollen characters is decisive in the determination of the variety. The ratio of empty pollen grains, the grain size and the density as well as the size of the pits on the surface are best suited to distinguish pollen lots.
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Relation between the germination and infection ratio on Sida hermaphrodita L. Rusby seeds under hot water treatment
117-121.Views:174Sida hermephrodita or virginia mallow is a perspective perennial herb in the Malvaceae family able to yield a biomass crop through between ten and twenty years. Additionally, the plants have a lot of uses and benefits for instance it can use it as a fodder crop, honey crop, ornamental plant in public gardens. It has favorable features like fast growing and resistance against the disease and climatic fluctuations, etc. Sida is in base stage of domestication therefore has a serious disadvantage the low and slow germination as a big part of wild plants. Due to the expressly low germination percent the need of seed showing of driller is should tenfold, 200 thousand seed/acre instead of 10-20 thousand what is not available and expensive Therefore practical purposes of our research of seed physiology was to increase the seed germination percent in a disposable ,basically wild Sida population. We examined two factors relating to seed germination percent and seed germination power during our research: the influence of hot water treatment and the effect of exogenus or endogenus infection of seed. However, in our germination tests, utilizing scarified seeds with hot water (65, 80 and 95 oC), 29,33 to 46% germinated of the seeds collected from the population of S. hermaphrodita in Debrecen. The average germination for all season was 5-10 % wihitout treatment and rised using hot water up to almost 50%. When physically scarified used, the oldest seeds showed the best germination (46 %) after the hot water operation in spite of the previus studys (Spooner 1985; Chudik et al. 2010; Doliński R. 2009.). We discovered that there are a distinguished close relationship between the seeds collecting time and the infection, as well as germination percentage. Thus, 2009 season was the most favourable in case of contamination (control:17,33 and 80 oC treatment:0%) as well as germination percent. It could be concluded that, the best season for our findings was 2009 due to autumn harvest of Sida seeds. In our oppinion, the autumn harvesting should be the best time to overcome the problem of the low germination and high infection percentage.
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Chemical characteristics of nectarine genitors used for breeding program in Romania
41-43.Views:117The geographic location of Bucharest area, the nature of its soil and its climate offer remarkably favourable conditions for nectarine growing, with juicy and well flavoured fruit, good for fresh consumption and processing.
The five years' investigation (1996-2000) has helped to identify some nectarine cultivars and hybrids (NJN 58, ARK 165, ARK 139, Morton, ARK 107, Romamer I, ARK 125, Firebrite) with exceptional fruit quality (dry matter content, titratable acidity, vitamin C content, overall sugar, and amount of pectic substances).
Dry matter content varied over the three years from 8.3 to 18.5% (NJN 68), meanwhile titratable acidity varied between 0.26 and 1.08% (Romamer I). Sugar / acidity ratio was within the limits of 8.6 and 36.5 (Nectared 7). Nectarines have high vitamin C content, over 10 mg/100g (Fairlane, NJN 67, Regina, Harko), glucides were found in Firebrite, Romamer I varieties and NJN 21, CIR1T127, HFSR3P4, HNA hybrids.
Some cultivars have been recommended for planting in this southern area of Romania (Crimsongold, ARK 125, Harko, Hardyred, ARK 134), others used as quality genitors (ARK 85, Nectared 7, NJN 21, ARK 21, ARK 134, Fantasia) in breeding projects.
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Yield and composition of supercritical fluid extracts of different Lamiaceae herbs: Satureja hortensis L., Ocimum basilicum L. and Melissa officinalis L.
75-79.Views:170In our work the effectiveness of supercritical carbon dioxide extraction was studied on volatile and nonvolatile components of Lamiaceae herbs. The aim of our investigations was to determine the optimal extraction parameters yielding high amount of volatiles and nonvolatiles in a desirable composition. As plant materials, dried and powdered cude drugs of Satureja hortensis, Ocimum basilicum and Melissa officinalis were chosen. Pressure (8-50 MPa), temperature (35-60 °C), time (5-60 min) and the ratio of modifier (5-50% methanol) of extraction were regulated. Results obtained by supercritical fluid extraction (SFE) were compared to that of the conventional extraction procedure, hydrodistillation (HD). In the case of Satureja hortensis, Ocimum basilicum and Melissa officinalis extract yield of SFE was comparable to the essential oil amount obtained by hydrodistillation from the same drug. Essential oil rich extracts were analysed by GC-FID, while extracts rich in nonvolatile compounds were analysed by HPLC. We have found that yield and quality of SFE extracts highly depend on the conditions of extraction.
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Evaluation of sour cherry varieties grown with environmental technology
39-41.Views:425The evaluation of a produce is an important moment of predicting its success on the market. The general impression, which is decisive, when a consumer chooses to purchase the commodity, should be interpreted in more objective, measurable terms. Primary data have been collected on fresh fruits derived from different growing technologies by organoleptic tests and the data filled up in the forms are processed with correlation analysis. The components were: taste, aroma, flesh firmness. The coefficients of correlation showed that the rest of characters as the appearance, juiciness, and the sugar/acid ratio.
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Effect of frost damage on leaf macronutrient status of eight apple cultivars in integrated apple orchard in Eastern-Hungary
37-40.Views:204The year of 2007 was critical for fruit growers in the region of Easter-Hungary. Several orchards were suffered frost damages. Significant frost damage was also happened in the orchard of Tedej Rt., which caused total fruit failure in the orchard. Our study was conducted in the integrated apple orchard (Malus domestics Borkh.) established on a lowland chemozem soil in East-Hungary, to investigate how frost changes the mineral content of different apple species. Leaf samples were collected 100 days after full bloom both in 2006 and 2007. The concentrations of nitrogen, phosphorous, potassium, calcium and magnesium were measured in leaves. The absence of fruits caused a smaller accumulation of nitrogen and magnesium in leaves. Falling down of flowers and fruit sets hindered the translocation of the uptaken phosphorous, potassium and calcium towards fruit sets. Due to fruit failure the vegetative processes became dominant. Leaves larger amount of phosphorous, potassium and calcium stored. Besides the absolute element content, the ratio of the different elements was also determined. Majority of calculated ratios were removed from optimal values due to frost. Both absolute content of nutrients and their ratios pointed out that the frost damage significantly affected the uptake of nutrients and their storing processes.
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Reactions of some cucumber (Cucumis sativus L.) lines and hybrids to zucchini yellow mosaic virus (ZYMV) and selection of tolerant breeding lines
66-68.Views:128In the past years zucchini yellow mosaic virus (ZYMV) has been appeared as a new pathogen of cucurbitaceous plants in Hungary. It caused severe disease outbreaks on the fields of pickling cucumber hybrids which are highly tolerant to cucumber mosaic virus (CMV). Preliminary inoculation tests have showed that all of the important pickling cucumber hybrids produced in Hungary were susceptible to ZYMV-P. However, a selection of Chinese Long and the breeding line I- KS 10C proved to be highly tolerant. The Fl plants of the crosses between the tolerant lines and the susceptible genotype H I were susceptible to ZYMV-P. F2 populations segregated for susceptible and tolerant individuals at a ratio of 3:1. The results strongly suggest that the resistance to ZYMV in our sources is controlled by a single recessive gene. Inoculations of the ZYMV and CMV susceptible cultivar Budai csemege and the CMV tolerant hybrid Perez Fl with the complex of ZYMV+CMV resulted extremely severe symptoms (strong mosaic and necrotic spotting of the leaves) on both cultivars. On the Chinese Long line, which is tolerant to ZYMV and CMV, respectively, the complex of the two viruses caused mild symptoms. The results show a synergistic pathological effect of ZYMV and CMV on differrent cucumber genotypes. More detailed studies on the interactions among the plant genotypes, viruses and virus strains are needed to develop cucumber hybrids that are highly resistant to the ZYMV+CMV complex.
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Determination of quality in stored pear fruits by chemical analysis and sensorial judgement
27-31.Views:241Aim of this research/project to determine the external and internal preferences of pear using descriptive sensory analysis, consumer preference. The research was performed on local consumers. Destructive measurements included fi rmness by puncture tests, soluble solids content (SSC), titrated acidity (TA). While there is a general positive trend for increasing preference with fi rmness, some consumers will prefer softer apples, and some will dislike the fi rmest pear. Sensorial judgement is able to classify the attractiveness, preference of properties specifi c for pear. Tests were performed by 13 persons on 4 pear varieties (Bosc kobak, Conference, Packham’s Triumph, Dessertnaia) checking 11 sensorial properties on a scale of 1 to 100 points. Relations of measurements and judgements were processed by correlation analysis. For analysis, the objects were furnished in 2011 from different growing sites (Csenger, Mérk and Nagykanizsa), taking from the store (in January) immediately. Among those the best notes were given to Bosc kobak and Conference coming from Mérk and to Packham’s Triumph grown at Nagykanizsa. It was stated that the success of sensorial judgement depends on the state of maturity, which is diffi cult to guarantee to be synchronous among samples of different varieties. Mature fruits are more praised as a sample of Bosc kobak taken from a chain of department store proved to be of balanced composition regarding its sugar/acid ratio (0.12) and the optimal fi rmness (5.75 N/cm2). Results of the correlation analysis suggest that the thickness of the skin is a decisive component of preference (r= 0.857), the typical pear flavour (r= 0.948), the taste as sweetness and acidity (r= 0.930 and r= 0.813). At the same time, no valuable relation could be detected between the data raised in the laboratory and the preference expressed by the sensorial tests, which should signalise that the opinion of consumers does not depend on any individual parameter obtained in the laboratory (sugar- or acid content, fi rmness) but rather on the complexity of several decisive components together (sweetness, acidity, fl avour, skin, etc.). The consumers’ preference cannot be measured objectively without the aid of adequate expertise and a prosperous surrounding, samples of optimal maturity and a thoughtfully edited judging form.
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The inheritance and durability of scab resistance in apple progenies
39-46.Views:128In order to select the appropriate parent cultivars and maintain the durability of resistance, it is important to clarify the mechanisms of inheritance of scab resistance depending on the parents. It has been known that the progeny segregation ratios based on scab-resistance do not depend only on the genotype of the resistance locus but also on the genetical makeup of the donor and recipient parents as well as on the susceptible parent.
The aim of this study has been to demonstrate what factors in the Vf, Vr and VA scab-resistant cultivars — combined with susceptible and resistant parents — affect the inheritance and durability of resistance in seedlings in their first 4 year's growing four years' growth. After inoculating apple seedlings sown in 2001 with the suspension of Venturia inaequalis (Cke.) Wint. in the greenhouse, we studied the segregation ratios of the progenies into reaction classes. Seedlings showing resistance in the greenhouse were also evaluated for scab-resistance after they had been moved to the field and had naturally been infected with the pathogen in 2002 and 2004.
The majority of our results obtained in the greenhouse test, similarly to earlier experiences, have not justified monogenic inheritance at the phenotypic level. The effect of susceptible parent cultivars on the segregation ratio of progenies have become apparent again. The high infection rate of seedlings in the field trials, which had previously exhibited varying degrees of resistance in the greenhouse test, has raised concern. Our data has raised further doubts, concerning the durability of Vf resistance in Hungary. It is assumed that the composition of natural field populations of Venturia inaequalis in Szigetcsép has changed. The complexity of Vf resistance has been confirmed. The high infection rate in the progenies derived from Vf resistant cultivars draws the attention to the importance of utilizing additional sources of resistance.
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Characterization of quince (Cydonia oblonga Mill.) cultivars using SSR markers developed for apple
7-10.Views:309Quince (Cydonia oblongaMill.) is a minor fruit crop, which is primarily used for marmalade, jam and sauce.Very few quince cultivars are known all over the world and in many cases similar names are used for presumably different cultivars. The aim of the present study was to evaluate and characterize the genetic diversity of 36 quince cultivars and selections with SSR markers. Seven out of 8 SSR markers designed from apple sequences could successfully yield amplification also in quince cultivars. Number of alleles per locus ranged from 2 to 3 alleles. These allele numbers are quite low when compared to apple. It is supposed to be the consequence of a genetic bottleneck. In spite of the low allele number per locus, the 36 quince cultivars formed 30 different genotypes. The ratio of homozygosity was low, which might be coupled with the self-(in)compatibility phenotype of quinces. SSR markers proved unable to differentiate putatively closely related cultivars (e.g. ‘Bereczki’ and ‘Bereczki bôtermő’). In general, the level of polymorphism among the tested quince genotypes was much restricted due to the low allele number detected. However, it must be considered that the number of analysed SSR loci is not enough high to estimate the overall heterozygosity of the quince genome. Further experiments are needed and the SSR markers proved to be a reliable and useful tool for such analyses.
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The effect of rootstock on the nectar production of apple cultivar `Idared'
15-25.Views:128Approaches based on the daily rhythm of apple flowers provide a new stage in nectar research, where the synchronous functioning of sexual organs is studied. In the flower biological studies the insect attraction of flowers was also studied. The two most important factors of insect attraction are the pollen- and nectar production of the flowers. From 1993 to 1998 we studied the food, that flower had to offer for the pollinating insects at different times of the day.
Studies were carried out on 'Idared', one of the hybrids of 'Jonathan' apple cultivar. The fruit of 'Idared' is bigger than the fruit of `Jonathan'. It is bright red, transportable, has a bigger productivity and is not subject to Jonathan-spots. Concerning its inner characteristics, it is juicy, the flesh consistency is better than that of 'Jonathan', but its acid/sugar ratio is worse (Sansavini et al. 1981).
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Effect of pre-harvest spray of calcium nitrate, boric acid and zinc sulphate on yield and quality of Nagpur mandarin (Citrus reticulata Blanco)
23-28.Views:261An investigation was conducted on uniform, healthy, eight year old trees of Nagpur mandarin (citrus reticulata Blanco) at Fruit Research Farm, Department of Fruit Science at College of Horticulture and Forestry, Jhalawar during 16 September, 2014 to 1 March, 2015. Various doses of calcium nitrate (1.0%, 2.0% and 3.0 %), boric acid (0.2 %, 0.4 % and 0.6 %) and zinc sulphate (0.2 %, 0.4 % and 0.6 %) were sprayed before harvesting and compared with untreated ones. The results obtained indicated that the trees sprayed with T27 i.e. (calcium nitrate 3.0 % + boric acid 0.6 % + zinc sulphate 0.6 %) showed maximum increase diameter of fruit, fruit weight, fruit volume, number of fruits per plant, fruit yield per plant, estimated yield per hectare, reducing sugar, non reducing sugar, total sugar, juice per cent, sensory score and reduced peel thickness over control. Further, T24 treatment combination (calcium nitrate 3.0 % + boric acid 0.4 % + zinc sulphate 0.6 %) has also significantly increased number of segments per fruit, TSS, TSS: Acid ratio, ascorbic acid content, and reduced number of seeds per fruit and acidity percent of fruits.