Vol. 12 No. 4 (2006)
Articles

New simple and efficient method of DNA isolation from pear leaves rich in polyphenolic compounds

Published September 26, 2006
Authors
K. Bokszczanin
Department of Pomology, Faculty of Horticulture and Landscape Architecture, Warsaw Agricultural University, 159 Nowoursynowska Str., 02-776 Warsaw, Poland
A. A. Przybyla
Department of Pomology, Faculty of Horticulture and Landscape Architecture, Warsaw Agricultural University, 159 Nowoursynowska Str., 02-776 Warsaw, Poland
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DOI
https://doi.org/10.31421/IJHS/12/4/673
Keywords
pear DNA isolation, polyphenolic compounds, tannins, copper (II) acetate, Pyrus
Dates
Published date: 2006-09-26
How to Cite

APA

Bokszczanin, K., & Przybyla, A. A. (2006). New simple and efficient method of DNA isolation from pear leaves rich in polyphenolic compounds. International Journal of Horticultural Science, 12(4), 21–24. https://doi.org/10.31421/IJHS/12/4/673
License

This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Abstract

This study aimed to establish a new protocol for DNA isolation from the Pyrus genus to get high quality DNA that is suitable for the generation of molecular markers, such as RAPD and AFLP. This method is based on modified CTAB extraction procedure (Aldrich & Cullis, 1993). For isolation of high-quality DNA we used copper (II) acetate treatment that enabled fixation and removing of tannins, present in abundance in Pyrus. DNA yield from this procedure is high. DNA is completely digestible with restriction endonucleases and amplifiable in the PCR, indicating freedom from common contaminating polyphenolic compounds.

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