Interactive effects of crop models, crop rotation and fertiliser application on the quality parameters of winter wheat grain yield in a long-term experiment on calcareous chernozem soil in the growing season of 2023. The results of our research showed that different intensity of technological models (different fungicide use, foliar fertilisers and growth regulators) had no or only partial (tri-culture) significant effects on the quality parameters (protein, starch, dry and wet gluten content, Zeleny index, grain hardness) of wheat. Protein and starch content showed a negative correlation. Higher values of protein, dry and wet gluten, Zeleny index and grain hardness were measured in the tri-culture rotation than in the biculture rotation. Of the quality indicators, only starch content was higher in biculture. The highest significant effect of fertilisation on the quality parameters was observed. The protein, dry and wet gluten contents increased with increasing fertiliser rates in the intervals 7.58–13.42%, 4.48–11.32%, 12.06–31.10% in biculture and 7.93–14.41%, 5.45–12.85% and 13.86–34.79% in triculture, respectively. In biculture, in the N200+PK (highest) fertiliser treatment, the wet gluten content reached the milling category I (31.13–32.10%), which is of good quality, while in triculture, the milling category I (31.22–32.62%) was already reached in the N100+PK fertiliser treatment, and in the N200+PK treatment, these values (34.55–34.79%) were in the premium quality category. In triculture crop rotation, protein, dry and wet gluten contents were significantly increased in the control (without fertiliser and foliar fertiliser) treatment due to the physiological activating effects of foliar fertilisers when super intensive technology was applied. Zeleny index and grain hardness values were lower in biculture (4.66–34.51 ml and 10.9–86.1, respectively) than in triculture (12.86–39.61 ml and 29.0–93.1, respectively), and significantly increased with fertiliser application in both rotations.