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Sequence stability at SSR, ISSR and mtDNA loci of common millet (Panicum miliaceum) from the middle ages
10-19Views:108Seed remains of medieval millet, recovered from a 15th century layer (King’s Palace, Budapest, Hungary), showed reddish yellow grain color after rehydrating on tissue culture medium that was close to grain color of modern cultivar Omszkoje. aDNA of medieval c. millet was extracted successfully, analyzed and compared to modern common millets by ISSR, SSR, CAPS and mtDNA. Analyses of fragments and sequences revealed
polymorphism at seven ISSR loci (22 alleles) and at the 5S-18S rDNA locus of mtDNA. CAPS analysis of the 5S-18S rDNA fragment revealed no SNPs in the restriction sites of six endonucleases TaqI, BsuRI, HinfI, MboI, AluI and RsaI. Sequence alignments of the restriction fragments RsaI also revealed
consensus sequence in the medieval sample compared to a modern variety. Morphological characterization of twenty common millet (Panicum miliaceum L., 2n=4×=36) cultivars and landraces revealed four distinct clusters which were apparently consistent with the grain colors of black, black and brown, red, yellow, and white. In the comparative AFLP, SSR and mtDNA analysis modern millet cv. ‘Topáz’ was used. AFLP analysis revealed that extensive DNA degradation had occurred in the 4th CENT. ancient millet resulting in only 2 (1.2%) AFLP fragments (98.8% degradation),
compared to the 15th CENT. medieval millet with 158 (40%) fragments (60% degradation) and modern millet cv. ‘Topáz’ with 264 fragments (100%). Eight AFLP fragments were sequenced after reamplification and cloning. Microsatellite (SSR) analysis at the nuclear gln4, sh1, rps28 and rps15 loci of the medieval DNA revealed one SNP (single nucleotide polymorphism) at the 29th position (A to G) of rps28 locus compared to modern millet.
Mitochondrial (mtDNA) fragment (MboI) amplified at the 5S-18S-rDNA locus in the medieval millet showed no molecular changes compared to modern millet. The results underline the significance of survived aDNA extraction and analysis of excavated seeds for comparative analysis and molecular reconstruction of ancient and extinct plant genotypes. An attempted phenotype reconstruction indicated that medieval common millet showed the closest morphological similarity to modern millet cultivar Omszkoje. -
Effect of agrotechnological treatment on nutritive value of common millet
65-69Views:162The cultivation of common millet shows an increasing tendency due to its adaptability to extreme poor circumstances. In addition, millet do not contain gluten forming proteins such as gliadin and glutenin, therefore people with gluten intolerance could consume it. The vitamin B and mineral content increase the importance of millet. The aim of our experiment was to measure the quality of flour of two millet varieties treated with different nitrogen fertilizer doses.
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Production technology development of millet at different ecological conditions
63-67Views:167The millet is a very special plant with good adaptation that gives the possibility for the late sowing and secondary production. However the effects of late sowing modifies the efficiency of the agrotechnological elements. The exainations – focused on this aspect – was conducted in the DU RINY and DU RIK in small plots in four replications in 2014. Among the examined factors (sowing time, nutrient supply, growing area) sowing time had the largest effect. The effectiveness of the agrotechnological elements decreases under unfavourable circumstances caused by the late sowing.
The agrotechnical elements modifies the yields in the examined genotype. Sowing time had the biggest effect on the yields of millet. The genotype „Maxi” had the highest yield in the different treatment variations.
The yield differences were significant between the sowing time and plant density variations, but the nutrient supply had not significant effect (the rate of precipitation was unfavourable in 2014 season).
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The importance of millet production in regional production, with special emphasis on climate change
141-146Views:185Regional production is a traditional production structure developed adjusting to the geographical, climatic, biological and soil conditions in given production regions, a certain territorial specification of agricultural production, and a type of farming that best fits the natural conditions and takes the biological needs of plant and animal species into account as fully as possible. The most probable element of risk in plant production is the changeable, extreme weather. That is the reason why the specific characteristics of the place of production and the characteristics of regional production should be considered to a greater extent. The establishment of the range of varieties appropriate for the place of production is the key issue in regional production. One of our historically grown cereal plants that perfectly fits regional production is millet. Due to its short growing season, favourable reproduction ratio and the fact that it is relatively undemanding, it used to be grown in larger quantities in the middle ages. Its good nutritional values made it an important food item, but over time, as a result of industrialisation and technological progress; it has been eclipsed by other cereal crops. In our country it is mainly used to cook porridge, but it is also used in the form of flour and as a base material in the spirit drinks sector. In the recent decades, millet has been applied only in a small area, mostly as a secondary crop in areas that dried out from drainage water in late spring, or as a replacement of extinct sowings due to its late sowing time. Water will be the most significant factor for the future of agriculture, especially considering climate change.My examinations took place in the area of the Institutes for Agricultural Research and Educational Farm of University of Debrecen, in the Research Institute of Nyíregyháza, in a small-plot experiment with four replications in 2016. -
Observation of some parameters on adult millet (Panicum miliaceum L.) individuals by pre-post herbicides controlling mostly grass type annual weeds
93-96Views:75The experiment was conducted in warmer mean daily temperature than the many years average, and almost in the same precipitation conditition as the many years average. The herbicides employed were sprayed in the 3-4 leaf stages developing phase of the millet (Panicum miliaceum L.) population by the dose of officially proposed. On the basic of the results, the herbicides didn’t effect deleteriously, in most instances, on the cultur plants in sort and long periodus at all. Tendenciously the values of parameters observed were sligtly better after the Stomp 330EC than the Dual Gold 960EC. ( The values of the parameters are significantly better at the treated variants than the control ones, are in realation with the wheather and development stile of the millet, beside the different weeding status of the plots.)
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Sorghum and millet as alternative grains in nutrition
91-95Views:273Sorghum (Sorghum bicolor L.) and millet (Panicum miliaceum L.) are the fifth and sixth most important cereal crops in the world. Gluten-free grains, therefore persons with coeliac disease could consume them also. In addition, they have a lot of positive effects due to their phenolic compounds (phenol acid, flavonoid, tannin). The total phenol content of sorghum is high, but Panicum miliaceum and Eleusine coracana have higher antioxidant activity. Fiber and mineral contents are also high, the protein contents are also higher than in standard cereals. Sorghum use is similar to corn: starch, glucose, syrup, and oil can be produced. Moreover, it can be used in preparing whole grain products, bread, pancake, dumpling, mush, cake, pasta and beer from sorghum. Broom and forage are also can be prepeared from them. Millet used such as mush, steamed food, cake, bread, alcoholic and non-alcoholic beverages.
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The impact of sowing date, production area and nutrient supply on the yield and quality parameters of millet
105-109Views:115The millet is a very special plant with good adaptation that gives the possibility for the late sowing and secondary production. However the effects of late sowing modifies to the efficiency of the agrotechnological elements. The examinations – focused on this aspect – was conducted in the DU CAS RINY in the small plots in four replications in 2013. The largest effect was recorded in the sowing time of the examined factors (sowing time, nutrient supply, growing area). The effectiveness of the agrotechnological elements decreases under unfavourable circumstances caused by the late sowing. The examined factors did not affect in the protein content and milling rate, except in the latest sowing time when protein content was significantly the highest.
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The influence of the fertilizing system on the petroleum residues biodegradation on a preluvosoil under control polluted
251-254Views:94The paper presents the partial results of researches regarding the agrochemical melioration of soils under control polluted by petroleum residues that took place at the Agricultural and Research and Development Station in Oradea, Bihor County.
The experimental device was made out of 1 m2 microparcels, spread out in subdivided parcels, in four repetitions, having tree factors: A - the pollution by crude oil from Suplacu de Barcău, B – the mineral fertilization, and C – the organic fertilization.
The experience was set out on a preluvosoil în the year 1993 and the soil was cultivated with millet in the first 3 years and with spring wheat in the last 7 years of research.
The researches carried out in Oradea had the objective of establishing the effects of the fertilizing system on the petroleum residue biodegradation on a preluvosoil under control polluted with crude oil from Suplacu de Barcău, Bihor County. -
Investigation of new seed mixtures in wildlife forage ground managem
9-13Views:204One of the most important method of habitat development is wildlife forage ground management. Many plant species can be grown on the crop fields, as mixture, too. Use of seed mixtures has not been spread on the native wildlife forage ground, however there are many advantages of them.
The aim of the research is to examine the role of the different plant species, seed mixtures in the game feeding, the savouriness of the plants of crop fields, as well as ascertain which seed mixtures are more suitable for wildlife forage ground management. On the basis of the investigation it can be find out that big amount of forage can be produced on mixed crop fields. Buckwheat, corn, rape, millet and white lupine proved to be the most beloved plants. It is suitable to make such mixed forages and use widely in the native wildlife forage ground management,
– which assures better feed conditions for the game,
– which are more attractive for the game and can draw away from other areas, so prevent, reduce game damage in forestry sector and in
agriculture that is in the forest manager’s, agricultural and game manager’s interests, too.
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Weed control with herbicide incorporation in sunflower
73-76Views:185During the last decade certificate registration of 13 active ingedients were removed by European Union from sunflower herbicide market, including the basis for the incorporating technology, the trifluralin active ingredient as well. Its relative, the benfluralin active ingredient, which include the Balan 600 WDG herbicide product in sunflower, will be sold again from 2015 spring in Hungarian pesticide market. It has a broad-spectrum and lond residue besides it has very high level selectivity on sunflower. It has very good effect against annual monocotweeds such as common barnyadgrass, foxtail species, large crabgrass and wild proso millet, dicotyledonous weeds such as common lambsquarters,and redroot pigweed. It has significant side-effect against common ragweed, black nightshade, wild buckweed and prostrate knotweed. The long effect residue provide the weed-free til harvest. Benfluralin is totally selective on sunflower, as no colouring, any deformation or growth inhibition was not observed during the entire growing season. It should be sprayed 3-4 days before sowing within 1 hour and to be incorporated into the soil in 4-6 cm depth with tillage equipment. It can be used in tank mix with fluorochloridon in incorporated technology against annual dicotyledonous weeds. After the Balan incorporation can be used postemergence timing imazamox and tribenuron-metil active substances against hard kill and deeply germinating weeds. The products can not be used in tank mix with bacterial products.
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Variation of boza nutrition parameters based on raw materials
67-70Views:178Boza is a ceral based fermented drink. Raw material of boza could be rise, millet, wheat and rye. Because of the diversity of raw materilas and the method of fermentation boza might show varied quality and nutrition value.
Nowadays healthy and conscious nutrition are playing more and more important roles in our lives. People pay an ever growing attention to the nutrition value of products, such as protein- and sugar content, components with antioxidant properties, and the mineral content of the products. In our research we wanted to know how the protein,-, sugar-, total polifenol-, mikro- and macro element content of products change.
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Examination of feral pigeon populations at animal breeding farms
52-56Views:120In the course of our examination, we assessed biometric data that determine gender, we evaluated sexual activity, measured the nourishment and necropsies of two feral pigeon populations feeding and nesting at animal breeding farms. The changes in the body weight of the different genders were significant on both farms (p<0.05).
The body weight and the crop volume of the birds were not significantly different at the examined farms.
On both farms, the elemental food source of the birds was the fodder and corn kernels given to cattle, and was supplemented by that of the pigeons living at the Debrecen farm with food from surrounding farms and with other seeds (pea, millet, rye).
By the examination of the genitals, the birds collected from the Nyírbátor farm showed sexual activity from the beginning of spring, and in the summertime decreasing activity is characteristic, while the pigeons from the Debrecen farm are only active from the summer months. In the course of the examinations in the Veterinary Institute of Debrecen, the birds proved to be free of parasites and the results of the Newcastle disease analysis were negative as well. The bacteriologic analyses showed a different proportion of E.coli, Pasteurella multicoda, haemolysing Staphylococcus bacteria at the two cattle farms. During the examinations for the statement of Salmonellas, this causative agent was stated only in case of birds that spend nights and nested in the town, which allude to human origin. We demonstrated Eimeria oocysts during the parasitological examination of the fecal samples in 5 cases at the Debrecen, and in 1 case at the Nyírbátor farm. -
Impact assesment of soil conditioners on a high clay content soil
137-141Views:170Our measurements were done in a soil conditioner experiment started in 2014 which was set in conventional tillage system at the Karcag Research Institute where a soil conditioner was used from 2010. Effect of two different soil conditioners on compaction, moisture content of the soil and on CO2-emission was studied. Measurements were done after sowing of maize and millet, and on stubble after harvesting. It can be established that less degree of compaction was characteristic to the soil of the plots treated for several years with the soil conditioner during the vegetation period than in case of untreated plots. Higher CO2-emission values were observed on the plots treated for several years than on the control plots. This effect can’t be established in case of soil conditioners used for first time in this year.