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Comparison of chemical parameters of enzyme active and inactive malt types
161-166Views:421Nowadays there is an increasing emphasis on the use of raw materials. Typically, raw materials – in this study malt – are used in animal feeds and used in the brewing industry. However, in terms of quality (eg. high fibre content), these can be included in human nutrition, we have limited information on this possibility. The aim of our work was to compare different malt flours and examine the possibility of using malt in the baking industry. We were to investigate some of the most relevant parameters, such as dietary fibre content, crude protein content, fat content, carbohydrate content, dry matter content, moisture content, salt and energy content. In the future, we aim to conduct a research on some of these parameters with different malt types as the brewing industry uses novel ingredients different cereals, pseudocereals such as amaranth (Amaranthus spp.), oat (Avena sativa L.), quinoa (Chenopodium quinoa Willd.) in addition to the spring barley (Hordeum vulgare L.) or wheat (Triticum aestivum L.). Based on brewing studies, malt has a high fibre and protein content. Having these advantageous qualities, malt should be part of humans’ healthy diet. Using malt flour in the baking industry can be a new direction which can lead to creating a healthier lifestyle and healthier eating habits than suggested by the WHO (World Health Organization).
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Mass occurrence of a Phoma-like fungus on common ragweed (Ambrosia artemisiifolia) in Hajdúság region, East Hungary
55-60Views:432Common ragweed (Ambrosia artemisiifolia L.) is one of the most important, allergenic weed species in Hungary. A. artemisiifolia invades both a broad range of often disturbed areas (brownfields) and either undisturbed ones like waste lands, roadsides, riverbanks and railway tracks. In field crops it can cause considerable yield losses mainly in sunflower, maize, cereals and soybean. In Hungary many inhabitants suffer from allergy caused by Ambrosia pollen which results a serious human-health risk. The aim of the control is to prevent flowering and seed propagation of A. artemisiifolia. Until now the occurrence of numerous pathogenic fungi which attack common ragweed plants have been identified in Hungary, however there is not yet available biological weed control program because of shortage in acceptable effectiveness, and endangering cultural plant species. During our weed surveys in the region of Hajdúság (East-Hungary) we found numerous common ragweed plants showing heavy necrotic lesions on leaves and stems. The objective of this study was to identify the fungus which was isolated from diseased tissues of common ragweed (A. artemisiifolia). The identification of fungus based on morphological characters of colonies and features of conidia and chlamydospores developed on malt extract agar (MEA) plates. After examination of axenic cultures we revealed that the fungus isolated from the leaves ands stems of common ragweed was a Phoma-like species.
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Identification of the Slovak traditional cheese “Parenica” microflora
227-239Views:428Numerous studies have demonstrated the higher accuracy, faster time-to-results and lower costs provided by MALDI Biotyper systems compared to classical methods. In this study, the culturable population of total count of bacteria, enterococci, coliforms bacteria, lactic acid bacteria (LAB) and microscopic fungi and yeasts from cow’s dairy products was identified using the MALDI-TOF MS Biotyper. Altogether, 50 samples of the Slovak cheese “Parenica” were examined. Total numbers of bacteria were cultured on Plate count agar at 37 °C for 24–48 h, aerobically; enterococci were cultured on Enterococcus selective agar at 37 °C for 24–48 h, aerobically; coliforms bacteria were cultured on Violet Red Bile lactose agar at 37 °C for 24–48 h, aerobically. The LAB were cultured on MRS (Main Rogosa agar), MSE and APT agar at 30 °C in microaerophilic conditions. The microscopic fungi and yeasts were cultured on Malt extract agar at 25 °C for 5 days, aerobically. Isolated strains (total 669) were subjected to identification by the MALDI-TOF MS. Among total count the identified bacteria mostly were Acinetobacter baumannii, Bacillus cereus, Micrococcus luteus and Staphylococcus warneri. Escherichia coli and Enterobacter cloacae were the most abundant coliform bacteria representatives identified. Coliform bacteria included Citrobacter, Hafnia and Klebsiella. Altogether three genera belonged to the LAB – Lactobacillus, Lactococcus and Leuconostoc were identified with Lactococcus lactis, Lactobacillus plantarum, Lactobacillus coryniformis, L. fructivorans and Leuconostoc mesenteroides were considered as the dominated LAB species in dairy products. Among yeasts, Kluyveromyces lactis, Candida zeylanoides and Yarrowia lipolytica were among the most isolated.
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Examination of the element content of beers
117-121Views:347Beer is a complex mixture of more than 800 several components, the most important ones are the minerals from this. Minerals originate especially from water and malt. In this work we measured the mineral contents of some own-brewed beer samples. We compared the results with the mineral contents of the water used as raw material of beer.
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The Role of Trichoderma in the Rhizosphere of Tomato Plants
67-69Views:158It is well established that microorganisms are closely associated with the roots of plants can directly influence plant growth and development. Species of Trichoderma, on the other hand are primarily studied for their ability to control plant disease. The ability of species of Trichoderma to directly promote or inhibit plant growth has been noted for many years.
Tomatoes were treated with different Trichoderma strains by seed treatment and soil inoculation. The Trichoderma were grown on malt-agar medium and conidia were washed off by sterile water for making suspension which contained 107 CFU/ml (colony forming unit/ml). The suspension was used for seed treatment and for the soil inoculation by watering as well. The artificial soil inoculation was made by Trichodermas growing on grounded maize were mixed in mould and tomato seed were sown in it. Tomato seeds were also sown in bags made of close-meshed material which allowed the soil microorganisms to colonize the roots but it simultaneously protected the roots from soil contamination. Roots were put on Trichoderma selective medium to check the root colonization of the Trichoderma.
The tomato plants were bedded out in a field in four repetition. After harvesting by hand the results supported by statistics shown that there was significant differences between the yield of the untreated and treated tomato plants by Trichoderma strains.