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Effect of fish feed containing two Chinese herbal extracts (Ganodema Iucidum and Lonicera japonica) on the non-specific immune system of Nile tilapia, Oreochromis niloticus (preliminary results)
9-14Views:132The effect of two Chinese herbs (Ganoderma lucidum and Lonicera japonica) on non-specific immune response of tilapia was examined. In addition to the control (no herbs), three diet variations were used. These contained 1.0% of Lonicera, 1.0% of Ganoderma and a mixture of Ganoderma (0.5%) and Lonicera (0.5%). The respiratory burst and phagocytic activity of blood leukocytes were monitored. Three weeks after feeding, the fish were infected with Aeromonas hydrophila. The results of this study showed that feeding tilapia with Ganoderma and Lonicera alone or in combination enhanced the phagocytosis of blood leukocytes, but not the respiratory burst activity. Both herbs, when used alone or in combination, reduced mortality after challenge with A. hydrophila. The highest mortality was observed in the control fish – 56.66%, and fish fed with Lonicera extract – 43.33% while 30% of fish died in the group fed with Ganoderma and the lowest mortality (20%) was observed when the fish were fed with a combination of the two herbs. It can be concluded that the herb extracts added to this diet act as immuno-stimulants and appear to improve the immune response and disease resistance of tilapia.
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The Evaluation of Grazed Grasslands on the Hortobágy
50-54Views:138The sward composition of different grasslands on Puszta Hortobágy has been developed according to prevailing abiotic and biotic factors. The abiotic conditions have been more or less constans for long periods of time, and the abiotic factors are determined by ecological conditions (climate, soil, topography). Among biotic factors grazing of herbivores was important in the development of Hortobágy grasslands for centuries (Sipos and Varga, 1993). Result of three-year investigations on the sward composition of grasslands utilised in different ways are presented. Data on ground cover, number of plant species, representation of different plant groups (grasses, sedge and bent-grass, herbs, legumes) and weeds are reported from six different grazed grassland types from Puszta Hortobágy.
In 1999-2001 a sward composition survey was conducted. Sample areas of 2x2 m2 were marked out in three replicates: on temporarily waterlogged grassland grazed by cattle (A), on dry grassland grazed by cattle (B), on dry grassland grazed by sheep (C), on dry grassland grazed by buffaloes (D), on dry grassland grazed by buffaloes and geese (E), on dry grassland cut for hay in May then grazed by geese (F). On the sample areas sward composition of grasslands was estimated according to Balázs (1949).
The average ground cover of different grasslands ranged between 60 and 100% (Table 2). The lowest value was found for grasslands C and E, which are grazed by sheep (C) and buffaloes and geese alternately (E). In these grasslands were some open spaces, on the other grasslands completely closed swards covers were observed.
The species diversity of these natural grasslands are high (Table 2). The grassland F, which were cut for hay in May had the lowest diversity (17-21). The highest number of species was found on grassland A and B (32-51), on other grazed grasslands (C, D, E) had 29-48 species.
The different plant groups had different representation in the total ground cover (Table 3). The number of herbs was always higher then that of grasses, but the cover of herbs was lower then that of grasses. The legumes and the sedge and bent grasses were present in high abundance in grassland A, but in the other grasslands were not.
The composition of herbs should be a warning for future utilisation systems on some grasslands of Hortobágy. Some species of herbs, e.g. Achillea millefolium, Artemisia vulgaris, Carduus acanthoides, Cirsium arvense, Cirsium vulgare Eryngium campestre, Galium mollugo, Galium verum, Ononis spinosa, Rumex crispus, Verbascum phlomoideus, Phragmites australis can be invasive on short grasslands. -
Microbial assessment of potential functional dairy products with added dried herbs
59-63Views:133The market of dairy products is a dynamically developing sector of the food industry. Potential, functional dairy products, made by adding herbs or spices, will have antimicrobial and antioxidant effect due to the active biochemical agents of the plant additives. Furthermore, these active components will widen the storage life of food products and enhance their organoleptic properties too. We worked out a technology for creating fresh cheeses using a gentle pasteurizing method by treating the mixture of raw milk and 1.5% fat contained in commercial milk. As herb additives, we used citronella (Melissa officinalis), and peppermint (Mentha x piperita) harvested by us and dried them via Tyndall-method in convective dryer on 40 °C for 5.5 hours per day. The drying period took three days. We bought dried citronella and mint from the supermarket, which were dried by ionizing radiation, to compare the microbiological pollution with the herbs dried by us.
The main target of this research was to create a microbiologically stable, potential functional dairy product. However, because of the bad quality of the raw milk and the gentle heat treatment we used for sterilizing bulk milk, or else, cheeses were not safe for human consumption. As a consequence, we need further studies to modify our technology and get a microbiologically stable product.
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The effect of herbs on the microbiological stability and nutritional quality of pariser
101-104Views:101The purpose of this study was to evaluate the functional properties of dried herbs (Thymus vulgaris L., Origanum vulgare L., Salvia officinalis L.) to improve the quality characteristics and microbiological stability of Hungarian meat product, pariser. The addition of herbs did not affect the microbial properties of the product. According to the Decree No. 4 of 1998 of the Ministry of Health, pariser can be considered safe. The nutritional quality of the different forms of pariser was also acceptable. However, the addition of oregano, sage and thyme changed the flavor characteristics of the parisers and the higher meat content would increase the final price of the product.
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The effect of dried herbs on the souring of sunflower oil
87-89Views:139Many herbs have antioxidant activity, which can replace the use of potencionally harmful synthetic antioxidants. Sunflower oil fortified with eight different plants and stored at room temperatur in my experiment. Specified intervals with determination of peroxide value, FFA, iodine value, anisidine value and TOTOX value followed the quality of the sunflower oil.
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HPLC-MS analysis of the active ingredients of chamomile (Matricaria chamomilla) bee feeding syrup
61-65Views:303Apiarists let prepare by bee honey products containing medicinal drugs. Our aim was to prove that the active ingredients originated from the herbs are also present in the bee products. This publication focuses only to the chamomile (Matricaria chamomilla) containing feeding syrup and the difference between the various syrup phases. To fulfil this task, we developed a method analysing the non-volatile components of the syrup using the flowers of chamomile. The method involves a filtration followed by HPLC-MS analysis. The analyses and the data evaluations proved that the non-volatile components of the herb were transferred to syrup. As the characteristic components of certain herbs could be identified also in the syrup, it is obvious that the components responsible for the medicinal effects are also transferred.
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The toxic effects of aflatoxin microorganisms in plants used as spices
59-62Views:100As an extension of the analysis of black, white and capsicum peppers for aflatoxins , we have examined an additional 11 types of spices and
4 herbs for these mycotoxins. The investigations consisted of assessment of the applicability of available methods of analysis and modifications of
these, where necessary together, with a limited survey of each spice and herb for aflatoxins. The analysis of 13 types of ground spices reported
the presence of low concentrations of aflatoxins in some samples of black pepper, celery seed, and nutmeg. We decided to include in our study 5
of the spices examined by these workers (cinnamon, celery seed, coriander, nutmeg, and turmeric) for a comparison purpose. In addition we
examined ginger, mace, cumin seed, dill seed, garlic powder, onion powder, and the herbs marjoram, rosemary, thyme, and sage. -
Comparative study of special honey products and herbhoneys
117-120Views:393Honey has a positive effect on human body due to its high content of biologically active substances (e.g. monosaccharides, vitamins, enzymes, amino acids, polyphenolic compounds). The properties of honey depend on its botanical origin due to the bioactive plant components, mainly secondary metabolites that are included in honey made by bees from nectar. Herbally infused honeys are delicious products that combine the therapeutic action of herbs and honey. Additionally, herbs can provide nutrient fortified syrup for honey bees and protect them against diseases and other ecological threats.The aim of this study was to define the physicochemical properties of multifloral honey, herb enriched natural honey and herbhoney samples. We measured the moisture content, pH value, electrical conductivity and proline content. Although great diversity was observed in the basic properties of the examined products. In our study, we found that the electrical conductivity shows the significant differ between the groups. All the samples, including the herbhoneys passes the quality standards of honeys. -
The examination of the marigold’s (Calendula officinalis L.) nutrient requirement in small-plot trial
61-66Views:194During our research we investigated the marigold's (Calendula officinalis L.) nutrient requirements with different fertilization treatments in small-plot trial. We measured the harvested marigold's drug's raw and dry weight on a weekly basis from July 6th until August 17th. We were using SPME (Solid phase microextraction) and GC-MS (gas chromatograph-mass spectrometer) to examined the effects of the different fertilization settings for the herb's main active ingredients of essential oil's percentage.
It was concluded, based on the results, the N30P40K60 fertilization setting is ideal in terms of the quantity of the marigold drug. Both the raw and the dry weights' measurements of the case, this fertilization setting have the most important effect on the herb's yield. The analysis of variance didn't show significant differences between the plots with different fertilization settings. We discovered relationship between the drying loss and the increasing quantities of nutrients. We think it may be possible the Alpha-thujon's and Alpha-cadinol's production and the drying loss's data are connected, which appear to confirm the N15P20K30 treatment's data.
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Usability of vegetable extracts in the protection against Alternaria alternata
113-116Views:149In our country, wormwood ragweed (Ambrosia artemisiifolia) may cause serious problems. Nearly 5 million hectares of agricultural area was infected with ragweed (Ambrosia artemisiifolia), which is believed useless weed. Allergological point of view, most problematic weeds adventive. However, many physiologically very beneficial compound also included, those with the effects have been known also by the Indians. On this basis, herbs can be thought of as ragweed. Our goal was to present that the ragweed contains antifungal active substances as well. In this paper we tested the biological activity of the extracts against Alternaria alternata F.00750 in vitro. We related based on our examination that ragweed contains biologically active agents, by which it is hampered the reproduction of the Alternaria alternata. The minimum effective concentration was 300 mg extract in a Petri dish, which was three days inhibited the growth of fungus. Full fungicidal effect was observed over dose 525 mg.
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Essential oil extraction from herbs and their use in the food industry
59-74Views:384Essential oil extraction of wild caraway and thyme was performed using a classical (HD) and microwave hydro-distillation (MWHD) and a laboratory supercritical fluid extraction (SFE) with a carbon dioxide as solvent. Our experiments demonstrated that the extraction yield of the essential oil performed in same conditions was influenced by the location of growth area; the maximum extraction yield of 10 ml 100 g-1 caraway was obtained from dried seeds collected from Csíkmadaras. This quantity far exceeded the yield of the Újtusnád samples. In the case of wild caraway (Carum carvi L.), the extraction method influenced thecomposition of the essential oil (carvone/limonene ratio), the highest limonene content being achieved by classical hydro-distillation. In the case of thyme, this effect was not detected, the thymol/carvacrol ratio was independent from the given extraction method. The obtained thyme essential oil possesses high antimicrobial activity demonstrated by agar diffusion test. The thyme extract provides a good protection against microorganisms collected on the surface of fresh vegetables following bacterial stains: Citrobacter portucalensis, Pseudomonas hunanensis, Pseudomonas baetica, Pseudomonas parafulva, Bacillus mojavensis and Enterobacter cloacae. Protective effect was also detected on the vegetable surface of by chitosan-based edible film coating during a 6-day-long storage period at a temperature of 4 °C. The caraway essential oil used as soft cheese seasoning with a direct, dilution-free method, proved to be unsuitable because the uneven distribution and confer a strong, unpleasant taste to the product in comparison with the ground wild caraway seed-dressed cheese.
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Economic Aspects of Rural Tourism
55-71Views:86necessary to ensure the livelihoods of those leaving the agricultural sector and to supplement the incomes of those working in agriculture. I research rural development in four settlements in Hajdú-Bihar County, in Balmazújváros, Hortobágy, Tiszacsege and Egyek, all bordering Hortobágy National Park.
There are many alternative income sources in settlements in the Hortobágy area, such as organic farming, production herbs, hungaricums, small animals and arts and crafts, as well as rural tourism. Rural tourism is not for subsistence, but a supplementary income source, mainly available during the summer time. I made a survey of rural hosts in the four examined settlements, and according to my results, I constructed a model reflecting the cost-benefit relation, as well as the payment period conditions of rural tourism. I am going to show whether it is worth dealing with rural tourism, and if yes, under what conditions. Rural tourism contributes to the maintenance of rural modes of living, in this way it has significant cultural, economic, ecological and social aspects, as well. It is crucial for settlements to create the appropriate conditions needed for joining rural tourism, if it is worth joining at all, and to realise investments for all these. Rural tourism has strict requirements for the levels of environment, infrastructure and services. Studying the Western-European practices, Hungary is lagged behind in the conditions of rural tourism and rural hosts have done their activities mainly out of necessity and not to maintain traditions. Rural tourism may result in success only by co-operation and over the long-run. -
The marigold (Calendula officinalis L.) drug essential oil agents change under different fertilization settings in small plot trial
57-60Views:182During our research we investigated the marigold's (Calendula officinalis L.) nutrient requirements with different fertilization setting in small-plot trial. We measured SPME (Solid phase microextraction) and GC-MS (gas chromatograph-mass spectrometer) we examined the effects of the different fertilization settings for the herb's main active ingredients of essential oil's percentage.
Based on the results, it was concluded, the essential oil agents' percentage breakdowns significantly depending on the cropping technologies. Besides that it is possible, based on Pearson's correlation test the marigold essential oil agents relationship can also be a major factor.
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Change of antioxidant compounds of spices during drying
77-81Views:170Spices and herbs have been used by humanity for thousands of years, so they are very important plants.
In this study, the change of dry matter content and antioxidant compounds of eight spices (basil, thyme, rosemary, mint, parsley, lemongrass, chives, coriander) have been examined the raw plants and in plants preserved by three different drying methods (an oven in 50–60°C; drying at room temperature; lyophilisation between -40 and -50°C, under pressure), because we wanted to see the change of the parameters.
The water content of raw plants was very high, i.e. the dry matter content was very low. By the application of the three drying methods nearly 100% of the water has left the plants, with the exception of the lyophilized basil and rosemary.
Based on the results related to the original material, lyophilized has proved to be the best treatment for the preservation of antioxidant compounds, however air drying also showed high results for some spices.
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The changes of the purple coneflower’s (Echinacea purpurea L.) herb and radix drug yield under different fertilization conditions
79-83Views:211In this research, the purple coneflower’s (Echinacea purpurea L.) nutrient requirement was examined under different fertilization conditions in a small plot experiment. We measured the medicinal plant’s raw and dry herb and root drug mass and drying loss under different fertilization settings and meteorological factors.
From the drug’s raw and dry mass perspective, based on the results, in our opinion, the control setting exceeded all nutrient settings’ results in 2016. In 2017 and 2018, the N75P100K150 nutrient setting has the biggest herb yield. As for the root yield, it was also the N75P100K150 setting which produced the biggest yield.
The Pearson's correlation test was performed to investigate the connection between the quantity of the raw, the dried herbs, the different nutrient settings and meteorological factors.