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Preliminary estimation of the efficacy of Fusarium sporotrichioides Sherb. as biological control agent against common milkweed (Asclepias syriaca L.)
Published June 30, 2018

A study of fungi responsible for severe leaf spots of common milkweed (Asclepias syriaca L.) in the Hajdúság region (East Hungary), Fusarium sporotrichioides and Alternaria alternata were isolated from infested leaf tissues. F. sporotrichioides was the most virulent fungus in pathogenicity tests conducted on healthy leaves of common milkweed ...plants. Inoculation of common milkweed (A. syriaca) in different growth stages with F. sporotrichioides yielded similar symptoms as the original ones. Spray mixtures containing 1.0×106 conidia/ml gave effective control when common milkweed plants were sprayed until runoff occurred. Laboratory (wet chamber) and field experiments showed that asexual spores of the fungal pathogen, F. sporotrichioides, exhibited bioherbicidal activity against common milkweed (A. syriaca).

More efficient control efficacy was observable on elder plants (at flowering stage) than younger ones. These results initiate that this fungus may be a biocontrol agent for controlling this invasive weed but should clarify its hosts because it could infect cultivated plants as well.

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The specification of the growing temperature on PDA of chestnut blight pathogen (Cryphonectria parasitica (Murr.) Barr) to optimize timing for biocontrol treatments by hypovirulent strains
Published June 14, 2017

The most destructive pathogen for the European chestnut trees is the blight fungus Cryphonectria parasitica (Murr.) Barr. The biological control is the only effective possibility to apply in situ biocontrol by hypovirulent strains against compatible virulent (wild) fungus strains. The infested bark tissues can inoculate by drilling holes surrou...nding and putting into agar discs interwoven by the appropriate vegetative compatible group (VCG) hypovirulent fungus strains. This latest can pass those virus-like particles (VLPs) by parasexual contact (called hypha-anastomosis)which responsible to hypovirulence. A laboratory experiment was made to find the optimal times to carry out the treatments. The intensity of growth of fungal colonies were analysed on different temperatures. The growth of the fungus on low temperature were rather slow,according to our expectations. On higher temperature the colony progress were the same as on the optimal 20–25 °C. These observations and the environment determine the date of the field applications under Hungarian weather conditions. It means the optimal treatment periods can be May or end of September to middle October in Hungary.

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