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Examination of microsatellite markers of Dorper sheep breed
57-61Views:176Number of not woolly and molty sheep exceeds 60 million throughout the world. Their numbers and their importance is growing, still they have appeared in the past two decades all over in North-America, Australia, New-Zealand and also in Europe. The South African Dorper has been a pioneer among them in Hungary. It was introduced in 2006 in the country. The Dorper sheep is the second largest breed in South Africa, which was developed from the crossing of Dorset Horn and the Blackhead Persian. The aim of the EU Member States in terms of this specific breed is increasing the small populations, improving the productive qualities, in addition to this avoiding inbreeding. However, finding appropriate breeding stock is difficult due to the small size of available populations and also to the suspected common of origin. With the help of various molecular genetic methods we could get a total view of the genetic background of these flocks. Nowadays the most commonly known and used genetic markers are microsatellites, because their applications give fast, accurate and easily reproducible results. There is no specific descriptive information on the genetic background of Dorper populations in the various EU countries , also regarding diversity between populations. Therefore in our work we want to optimize the conditions of applicability of 31 selected microsatellite reactions as a first step of mapping the entire genetic background of the different EU Dorper populations.
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Seasonal variations in somereproductive parameters of Dorper Rams in Hungary
17-20Views:235The purpose of the present study was to test the hypothesis that season has an affect on semen quality and scrotal circumference of Dorper rams. The experiment was carried out with six Dorper rams aged between 15 and 18 month. Semen samples were collected with artificial vagina and volume, concentration (x 109/ml), total sperm number/ejaculate (x 109), mass motility (0–5), progressive motility (%), scrotal circumference (cm) was observed. Significant differences (P<0.05) were observed in concentration, total sperm number/ejaculate, scrotal circumference in different seasons. Volume was the highest in autumn (1.4±0.5 ml) and the lowest in the spring (1.3±0.4 ml). Concentration of semen was lower in spring (2.6±1.5 x 109) and summer (3.3±1.5 x 109) as compared to fall (4.1±1.1 x 109) (P<0.05). Regarding total sperm number/ejaculate (x 109), scrotal circumference (cm) all the seasons differed significantly (P<0.05), although the season had no effect on mass motility and progressive individual motility (P<0.05). In conclusion the present study showed that semen quality parameters and scrotal circumference of Dorper rams were better in autumn than in the other sea- sons.
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Ovarian performance of Barbados Blackbelly and Dorper crossbred ewes in the mirror of body conditions
23-26Views:75The aim of examination was to detect the puberty and to follow the ovarian cycle of Barbados Blackbelly×Dorper ewes in connection with their body conditions and the effects of meteorological parameters. The blood samples had been taken from 17th of November 2008 untill 08th of June 2009 for analyzing the progesteron concentrations, establishing whether they showed perpetual ovarian performance or not. The end of examination
was timed due to releasing the ram in middle of May 2009.
The daily avarage bodyweight-growth is well featured by the bodyweight tendence. The body conditions were evaluated by ultrasound tissue examination.
During the full length of the examination the avarage daily temperature, the avarage humidity level and also the number of daily sunlighted hours were served by the Agrometeorological Observatory of the University of Debrecen.
The bodyweight tendence and the meteorological parameters were correlated to the breeding season of ewes. In conclusion the bodyweights and their tendences at the young age and the body condition parameters measured at certain anatomical areas can influence the length of the breeding season of the ewes. However the number of daily avarage sunlighted hours, the daily avarage temperature and also the humidity did not effect the cyclic ovarian perfomance of the Barbados Blackbelly×Dorper ewes in Hungary. -
The effect of genotype and the location of sampling on the mineral content of wool
157-160Views:182Mineral supplementation is very important in high producing farm animals. The estimation of exact mineral intake is very difficult in forage eating animals, like sheep. Accessing of long term mineral status seems to be possible using wool mineral analysis. However several factors can affect the results. Therefore, the aim of this study was to test the effect of breed and sampling location on the mineral content of sheep wool. 20 Dorper and 20 Tsigai sheep were chosen from the same farm. Samples were obtained from 3 locations (withers, side and quarter) and tested for 8 elements: Ca, Mg, Na, Co, Cu, P, S, Se , Zn. The samples were cleaned with ethyl alcohol from organic contamination, then after adding nitric acid were mineral analized using ultrasonic cleaning unit. The samples were analysed with ICP-OES (Perkin-Elmer, Optima 3300 DV). Statistical analyses were carried out by GLM procedure of SAS statistical analyses software. Differences between means were checked with Tukey-test. Significant breed differences were detected in the case of Mg, Na, S, Se in spite of the same feeding regime. The wool mineral content were within the reference range. The sampling location had no effect on the mineral content of wool.