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Surface polishing method application to reduce micotoxin content of mill wheat
Published November 13, 2012

The fungi causing the infection and most of the harmful toxins they produce are concentrated in the bran of the grain, thus the intensive surface cleaning, the so-called debranning operation could allow the reduction of contamination in the milling technology. The essence of the PeriTec technology – originally developed by SATAKE, a Japanese, to clean rice – is that it gradually removes the bran layers of the grain by mechanical means before further processing. We modeled the PeriTec technology with a laboratory size, batch-operating, horizontal debranning machine by SATAKE. The flour, milled grain after grinding 40 sec, the initial toxin content was only a small proportion (~15–20%) measured. The results showed that below the limit of DON toxin contaminated wheat (DON: 1.15 mg kg-1) during the grinding surface of the detached bran toxin contamination shows a very high (6.16 mg kg-1). The 40 seconds debranning before grinding shows lower DON toxin content than without debranning. So it is importance before the grinding. The toxin contamination of the bran fractions is significantly reduced, which is importance to the feeding point.  As a result of debranning, the toxin content of the grinding fractions decreased, which justifies that that PeriTec method is suitable for the reduction of toxin contamination. 

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Wheat cleaning and milling technologies to reduce DON toxin contamination
Published December 15, 2019

Mycotoxicosis caused by Fusarium fungi holds a huge risk considering economic and food safety issues worldwide. By applying milling technologies, we attempted to reduce the concentrates of DON toxin, as it is the most often found toxin in wheat.

The processes of sieving, aspiration and combination had been used on wheat with high DON concentration. As a next step, grains were sorted using a horizontal cylinder separator, assorted by an optical and a gravity separator, and finally, the products were scoured and ground. The contamination level of the wheat and flour samples were defined by the HPLC-MS method.

Regarding the results, it can be stated that toxin concentration was most effectively reduced by optical separation and scouring, and by applying these milling techniques, food safety can be increased significantly.

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A tárolási feltételek hatása a kukorica Fusarium fertőzöttségére és toxin szennyezettségére
Published March 27, 2002

Corn samples harvested in 1997, 1998 and 1999 from different soil types were stored at different conditions (temperature, moisture content, state of kernels) for six months. The Fusarium contamination was examined by plate dilution method and the amount of mycotoxins (F-2, T-2, HT-2, DON, DAS) were determined applying HPLC method immediately af...ter harvesting and in the third and sixth month of storage. The aim of our study was to find correlation between the ecological factors, storage conditions and the examined parameters mentioned above, as well as to prove them statistically. According to the examinations carried out after harvesting we could state that the soil type had no effect on the parameters. Analysing the effect of the years we found considerable differences. The Fusarium infection of corn samples in 1998, while the toxin contamination in 1999 was the highest. The results of storage experiment show that year (number of microscopic fungi, F-2, T-2, HT-2, DAS and total toxin content) and moisture content of kernels (F-2, T-2, and total toxin content) have a significant effect on the examined parameters. We could prove the effect of temperature on the T-2 content (samples with natural moisture content) and DON content (samples with 14% moisture content). Higher values were found at higher temperature storage. The ratio of damaged kernels influenced the DON concentration in the non- wetted samples and the number of microscopic fungi in the wetted samples.

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Utility of Winter Wheat According to Visual and Microbiological Fusarium Infection, as Well as Toxin Examination
Published November 26, 2003

In 1998 the Fusarium infection was studied visually and microbiologically and also F-2, T-2, HT-2, DAS and DON contamination were measured using 22 winter wheat samples. The correlation between the different parameters of 22 wheat samples were determined by regression analysis. According to our results we can state the following.
There is no... significant connection between the results of visual, microbiological and toxicological examinations. This means that no certain conclusion can be drawn about the toxin contamination of samples – which is a determining factor of its utility – based on the visual symptoms and the plate dilution method.
Our results indicate however – though it is not proven statistically – that those samples in which the Fusarium infection did not exceed the limit of the standard, also had low toxin contamination, therefore they can be used as components of forage.
It is a considerable problem, however, that according to the visual qualification, such samples are excluded from the later utilisation, wherein the toxin contamination does not justify such action. Therefore, it is necessary to examine the toxin content of those samples which show high infection by visual symptoms. To reducing the number of expensive toxin examinations it would be advisable to change the currently used 0,5% limit which is indicated in the standard for a higher value of infection, for example to 2%, as recommended by Mesterházy (1998).

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Ecotoxicological impact of DON toxin on maize (Zea mays L.) germination
Published February 25, 2014

Fusarium graminearum is one of the most significant arable pathogen in Hungary, and various types of trichothecene mycotoxins (mostly DON, deoxynivalenol) are detected most commonly in cereals (Biró et al., 2011). Fusarium infection and mycotoxin production could not be eliminated, and infected maize by Fusarium sp. cannot be exploited as food..., seed, or animal feed. However it can be raw material of biogas production. In this research we would like to investigate the content and effect of the toxin in the end product of biogas production on plant germination. The Fusarium sp. can cause mildew and seedling mortality in seed of maize (Zea mays L.), so we examine the effect of this on germination. In preliminary examination Fusarium sp. was not detected in the bioreactor of the Institute after the retention time (30 day), however it can be assumed that during the hydrolysis of the fungus growth and mycotoxin production also increased exponentially. There were no appropriate tools to detect the toxin in the end product of biogas production so modelling of anaerobic hydrolysis was necessary. The effects of hydrolyzed product for germination were also detected.

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