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Development of a new measurement method to determine plant antioxidant status
105-112Views:214The antioxidant capacity of 12 cultivar that were harvested in 2014, were determined by FRAP (Ferric Reducing Ability of Plasma), DPPH (1,1-diphenyl-2-picrylhydrazyl radical-scavenging activity) TEAC (Trolox Equivalent Antioxidant Capacity) and photochemiluminescence method. In sour cherry, the most antioxidant effects of natural bioactive compounds are anthocyanins. Our results show that the photochemiluminescence method is the most suitable to determine the antioxidant capacity of red soft fruits and tart cherries
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The significance of sour cherry in iron supplementation
101-104Views:187The iron concentration of ’Csengődi csokros’, ’Debreceni- and ’Érdi bőtermő’ , ‘Éva’, ’Kántorjánosi’ ,’Petri’ and the ’Újfehértói fürtös’ cultivars was determined by ICP-MS. Furthermore the Vitamin C, L-Malis acid and Citric acid concentration of samples were measured.
Our results show that large amount Fe2+ (average 20.5 mg kg-1) accumulates in the pulp of sour cherry. Besides, the concentration of Vitamin C, L-Malis acid, that increase the absorption of iron, are high. Based on these results, the sour cherry and the products of sour cherry play an important role in the forming of iron content in body.
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Sour cherry seed as an industrial gamma tocopherol source
27-33Views:192In our experiments sour cherry kernels were investigated. The kernels of different sour cherry cultivars were originated from Újfehértói Gyümölcstermesztési Kutató és Szaktanácsadó Nonprofit Közhasznú Kft. Tocopherol profile, oil yields and nutritional value were stood in focus of examinations.
We started processes preparing of sour cherry kernels. After the preparation recovered non-polar fraction was investigated. We worked with formerly developed chromathographic system, which is capable to separate different tocopherol isomers efficiently. We measured concentration of each tocopherol isomers and registered those and their relation in different oil samples.
According to received data, alpha-, gamma- and delta-tocopherol appeared in samples, but beta-tocopherol could not be detected. It became clear that level of gamma-tocopherol is far higher than alfa- or gamma-isomer, it is concentration exceeded 1–1.5 mg ml-1.
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Sour cherry as a functional food
41-47Views:190The antioxidant capacity of ’Debreceni bőtermő’, ’Újfehértói fürtös’ and ’Érdi bőtermő’ cultivars were determined by FRAP (Ferric Reducing Ability of Plasma), DPPH (1,1-diphenyl-2-picrylhydrazyl radical-scavenging activity) and photochemiluminescence method. In sour cherry, the most antioxidant effects of natural bioactive compounds are anthocyanins. Our results show that the photochemiluminescence method out of applied assays is ratheris suitable to determine the antioxidant capacity of red soft fruits and tart cherries. The correlation is good between the determined anthocyanin concent by this technic and pH-differential spectrophotometry. However, both FRAP and DPPH assays are inaccurate.
The anthocyanin composition of ’Debreceni bőtermő’, ’Újfehértói fürtös’ and ’Érdi bőtermő’ ’Csengődi csokros’ sour cherry varieties were analised. There are big differences between the accumulation of anthocyanan compounds of cultivars. ’Csengődi csokros’ produce melatonin in large quantity. On the evidence of the results, we can say that the hungarian sour cherry cultivars are suitable for functional food development.
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Definition antioxidant activity of selenium-enriched food sprout, as well as their microbiological analysis
25-30Views:255In this present study, we prepared selenium-enriched food sprouts, where the antioxidant capacity was analysed, we also determined their microbiological status. We took into account the fact, we choose micronutrients to our treatment, that selenium can be delivered to the body by a small amount with the most widely consumed food.
We focused during our research to determine that the increasing concentrations of selenium treatment, in which we used sprouts, knowing fully well that it has an impact on aboriginal antioxidant capacity of sprouts, which is mainly due to high vitamin content of sprouts.
Furthermore, we think it is important to make microbiological analysis, because germination conditions, for example temperature, pH, all this will create an ideal environment for the growth of microorganisms. So we had goal to determine, how the used selenium concentration affect the total plate count, coliform bacteria count and Staphylococcus aureus count of sprouts.
We determined the aboriginal water-soluble and lipid-soluble antioxidant capacity of sprout with the PHOTOCHEM chemiluminometer and we applied pour plate technique for the mapping of the mycrobiological state of sprouts.
Experimental results are evaluated, we state that increasing concentrations of selenite or selenate treatment, is primarily water-soluble antioxidant capacity of sprouts was affected. The water-soluble antioxidant capacity of wheat sprout was much higher than the measured values in pea sprout, this may be linked to what we measured. That is much higher ascorbic acid content in case of wheat sprout, which is well known as one of the most important antioxidant properties compounds of wheat sprout.
We conclude from the results of the microbiological, that the highest concentrations of selenite or selenate treatment has a relative significant anti-microbial effect in case of wheat sprouts. Coliform and total plate count showed no clear decreasing tendency, although the values of treatments in both cases obtained were below the control values.
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Effect of anthocyanin-rich Hungarian tart cherry extract on blood antioxidant status in C57BL/6J mice
335-341Views:220In this study male C57BL/6J (B6) mice were used to determine the possible effects of our Hungarian tart cherry extract in a chronic obesity mouse model on antioxidant capacity.
The control group received standard mouse chow, the high fat control group was switched to high fat diet and tap water supplemented with 5% sucrose. The high fat+anthocyanin group received the high fat and sucrose diet, but dissolved in the drinking water they received anthocyanin-rich tart cherry extract. After six weeks, the antioxidant capacity and SOD activity were measured. Antioxidant capacity was decreased with a significant elevation of SOD activity. The tart cherry extract made a significant enhancement in antioxidant capacity and SOD activity. Our results show that chronic anthocyanin intake has a potential to enhance redox status associated with obesity.