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Microbiological quality of bulk tank raw milk from two dairy farms in Hajdú-Bihar County, Hungary
105-112Views:531Two main channels have been identified to be responsible for microbiological contamination of raw milk and milk products. Firstly, contamination has occurred due to udder infection from the cow or the blood which harbours most bacteria that come in contact with the raw milk. Secondly, via external factors (may include faeces, skin, contaminated water, environment etc.) which are associated with the operation of milking. There is direct contact with the milk and/or surfaces before, during or after the milking, posing public health risk and economic decline. The aim of this study was to examine the bacteriological quality of bulk tank raw milk samples collected from two different size dairy farms (Farm 1 and Farm 2) of different housing forms (cubicle loose and deep litter) in Hajdú-Bihar County, Hungary in July, 2017. Three samples were taken from each farm, and the total plate count, coliform count, Escherichia coli count, Staphylococcus aureus count, and yeast and mould count were determined in them.
The results clearly showed low level of all measured bacteria group load in Farm 1 samples in comparison to Farm 2 with the exception of coagulase-negative Staphylococcus (CNS) which represented high level in general, indicating significant difference (P<0.05). The mean value of total plate count in Farm 2 samples was higher (1.0 × 105 CFU/mL) than Farm 1 samples (2.8 × 104 CFU/mL). There was a significant difference (P<0.05) in mean count of coliforms in raw milk samples between Farm 1 and Farm 2. Similarly, results of E. coli were significantly different (P<0.05) with mean count of 1.44 × 102 CFU/mL and 2.02 × 103 CFU/mL for Farm 1 and Farm 2 respectively.
Results of Staphylococcus aureus also showed significant difference (P<0.05) with mean count of 9.7 × 101 CFU/mL for Farm 1 and 6.28 × 102 CFU/mL for Farm 2. The mean of mould count recorded was 1.07 × 102 CFU/mL and 4.93 × 102 CFU/mL for Farm 1 and Farm 2 respectively. The recorded mean of yeast count was 1.68 × 103 CFU/mL and 3.41 × 103 CFU/mL for Farm 1 and Farm 2 respectively; however, both farms showed no significant difference (P>0.05) in terms of mean of mould and yeast count. Although Farm 2 produced six times lower milk quantity than Farm 1, the measured microbial parameters were high. Both farms’ microbiological numbers were higher above the permitted limit values as stated by Regulation (EC) No 853/2004, Hungarian Ministry of Health (MoH) 4/1998 (XI. 11.).
This could be an indication of non-conformance to effective GMP, ineffective pre–milking disinfection or udder preparation, poor handling and storage practice, time and temperature abuse and inadequate Food Safety Management System Implementation. Therefore, our recommendation is as follows; establish control measures for pre- and postharvest activities involved in the milking process which would be an effective approach to reduce contamination of the raw milk by pathogenic microorganisms from these farms, strict sanitation regime and hygiene protocol be employed and applied to cows, all equipment, contact surfaces and minimize handling of the milk prior, during and after milking. This will also serve as scientific information to the producers for continual improvement in their operations.
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The effect of washing for the shelf-life parameters of spinach (Spinacia oleracea L.)
131-135Views:318Spinach is a very popular green leafy vegetable because of its versatile usage and beneficial for the health. However, spinach may contain several pathogen bacteria: Escherichia coli, Klebsiella spp., Salmonella spp., Enterobacter spp., Citrobacter spp., Shigella spp. and Listeria monocytogenes, which can cause several serious health problems. This study investigates the effects of washing with citric acid for the shelf-life parameters of spinach in comparison to the effect of washing with water and control. Washing of spinach with 0.5% citric acid solution decreased the elasticity of the spinach leaves, as well as the chlorophyll content. On the other hand, the total plate count, as well as the yeast and mold count could be decreased with this treatment, but difference was not detectable at the forth storage day. The fecal indicator E. coli did not change, indicating washing was not effective in this case. Further optimisation of treatment and storage conditions may decrease microbial risk of fresh spinach consumption without decreasing its sensory quality.
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Comparision of growth of mature all-female and mixed-sex Common carp (Cyprinus carpio L.) stocks in RAS
65-68Views:739The common carp (Cyprinus carpio L.) is the most important fish species in Hungary, it is more than 70% of the total Hungarian fish production. The common carp production is important not only just in Hungary but in Middle-East Europe, as well as Southeast Asia. Majority of the production comes from fishpond culture. If the production sector wants to meet the increasing customer demands, there is need to intensify research on the intensive fish production opportunities for example all-female common carp technologies. The all-female technology is one of a genom-manipulation technology. Its production showed better growth rate than mixed-sex population in pond culture. Our experiment combined the recirculation aquaculture system (RAS) and the all-female common carp stocks intensification technologies. The reason for the experiment, is that there are no result about the growth of all-female common carp growth in RAS.
The experiment used the „Tatai grey scale type” common carp stocks. We propagated two all-female stocks (T2 and T3) and a control group (TK). Due to technological characteristics of RAS, the water quality parameters were the same for all treatments and corresponded to the technological tolerance of common carp.
The experimental period was from July 10, 2019 – November 20, 2019. Result of growth performance showed that the growth of mixed-sex stock was significantly higher than all-female stocks; (Control=3692.0±590.5g, T2=3438.8±415.4g, T3=3294.1±659.1g). Feed conversation ratio (Control=1.3±0.1 T2=1.5±0.2 T3=1.6±0.5) and SGR% (Control=0.8±0.0 T2=0.7±0.1 T3=0.7±0.1) were similar.
By the results it can be said the all-female common carp technology has neither advantages nor disadvantages compared to the mixed-sex stock. The all-female technology can be beneficial if the consumers need female common carps. It is worth continuing the experiment and examine how the stocks will perform above 3kg body weight.
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The effect of different fat content fish feed on the production parameters and meat quality of the hybrid striped bass
33-37Views:443The interspecific ’hybrid striped bass’ is the result of cross breeding. Their offspring have got good chances of growth and tolerate extreme water temperatures and the content of dissolute oxygen more than their parents. Furthermore, they are more resistant to diseases. Therefore, the hybrid is perfectly suitable for intensive fishery production.
Our experiment lasted for seven weeks. We analysed the indices of growth and production of the hybrid as the consequence of nurturing them with two feeds of high nutritive value during this period. We examined the content of fat and of fatty acids in the flesh of these fish. There were no considerable differences in terms of weight gain, feed conversion ratio and uneven growth between the two groups, while survival was less likely among the the fish which had consumed more fat. We demonstrated, examining the flesh of the individuals which had consumed feed containing lower fat content, that it was fattier and contained more dry matter than those that had been fed of higher fat content. Besides, the content of omega-3 and omega-6 fatty acids was higher in the fish that had consumed less lipids.
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Product orientated sewage sludge composting technologies in the practice of AKSD Ltd.
33-36Views:338In recent years the regulations of the EU unambiguously determine that the biodegradable wastes should be used for biogas production or composting. The direct use of sewage sludge in agriculture is limited by its hazardous microelement content, but it can be used effectively in composting. The limitation parameters can be extinguished with correct compost-mixture and technology variants. The exact determination of the technologies and mixtures result a stable, constant quality product. The compost, as a classified product, is friable structured. The end-product is a
homogeneous mould with good water capacity, does not contain any weed seeds and pathogenic organisms. The compost does not have a acidify effect on the soil. It is an effective fertilizer thanks to its macro and microelement content. -
Examination of the Effect of Cropyear on the Yield Potential and Yield Stability of Winter Wheat Varieties
62-67Views:293Variety selection is one of the most important, determinative elements of sustainable winter wheat production. Yield potential, and yield stability are the most important elements in the variety selection of winter wheat, but baking quality parameters play an important role, too.
Several winter wheat varieties were tested for yield and yield stability on chernozem soil in the Hajdúság (in the eastern part of Hungary), in the 2001-2002-2003-2004 cropyears. The management factors were the same for all cropyears. 15 varieties in early the maturity group, 14 varieties in the middle maturity group and 4 varieties in the late maturity group were tested in the above mentioned cropyears. The climatic conditions were average in 2001, dry in 2002, extremely dry in 2003, and very favourable in 2004.
We obtained 5298-6183 kgha-1 yield from early maturity varieties, 5683-6495 kgha-1 from middle, 5694-6031 kgha-1 from late ones in the average of four years. The cropyears had strong influence on the yields, even on chernozem soil, and were characterized by excellent water – and nutrient – husbandry. Averaging of cropyears and genotypes, we obtained 6984 kgha-1 in 2001 (average cropyear), 5452 kgha-1 in 2002 (dry cropyear), 3120 kgha-1 in 2003 (extremely dry cropyear) and 8400 kgha-1 in 2004 (optimum cropyear), respectively. The yield differences between the minimum and maximum yields were 885 kgha-1 in early varieties, 812 kgha-1 in middle and 337 kgha-1 in late maturity varieties, respectively. The varieties characterized by high yield potential and the varieties characterized by good yield stability were different, so in variety selection we have to take both genetic traits into consideration. There were positive, significant correlations among the yields of winter wheat varieties (early, middle, late), the temperature of spring months. (March-April), and the rainfall of spring months (March-April) (R2=0,703**-0,768** and R2=0,681**-0,749**, respectively). We found a high negative correlation between the temperature of early summer months (May-June) and the yields of wheat varieties (R2= -0,856**- -0,918**).
According to the results of our experiment, it is very important to harmonize yield potential and yield stability in the variety selection of winter wheat. -
The effect of hybrid, nutrient-supply and irrigation on the grain moisture content at harvest and the starch-content of maize (Zea mays L.)
89-95Views:192Maize is a worldwide dominant plant. According to nowadays plant production principles it is important to investigate and optimize the site-specific nutrient-supply and other production factors, such as hybrid and irrigation, in the case of this plant as well.
At the Research Institute of the University of Debrecen, Center of Agricultural Sciences and Engineering, at Látókép the effect of nutrient-supply and irrigation on the quantity and quality parameters of different hybrids were investigated in a small plot long-term field experiment. In this paper we introduce the results regarding the corn moisture-content and the starch content of the yield.
We have chosen three maize hybrids – that have been bread in Martonvásár – for our investigations. The effect of macronutrients is investigated in this experiment on five levels. The half of the experimental area can be irrigated during the vegetation period – whenever it is needed – by linear irrigation equipment, but on the other half only the water amount originating from the precipitation can be used by plants.
In the year 2008 the hybrid affected the grain moisture content at P=0.1% level, while nutrient-supply had an effect at P=10% significance level. We haven’t revealed either any effect of irrigation or of interrelationship between production factors. It can be stated that there are differences between the hybrids on each nutrient-supply and on both irrigation levels. The grain moisture content increased parallel to the longer vegetation
periods.
The starch content of maize is mostly affected by the hybrid,
so on P=0.1% significance level. Regarding our results, it can be
stated, that the starch content shows a decreasing tendency
parallel to the longer vegetation periods. -
Early evaluation of use of fermented chicken manure products in practice of apple nutrient management
195-198Views:506According to the Green Deal efforts, the importance and relevance of organic fertilization will increase in the near future. Therefore, the investigation of the effects of different organic fertilizers on soil productivity and nutrient supply is a priority area of agricultural research. Organic fertilizer experiment was conducted in an eight-year-old apple (Malus domestica Borkh.) orchard at Debrecen-Pallag. In the trial Pinova cultivar was used. In this study, two different fermented chicken manure products were added to the soil (in 20 cm depth) to test their effects on soil nutrient status, plant uptake and fruit quality. It was found that the applied treatments slightly increased the pH and nutrient levels in almost all cases, but significant effect was not observed in all treatments compared to the control. Leaf nutrient contents (N, P, K, Ca and Mg) were measured in the experiment. Leaf nutrient status was not affected by the fermented chicken manure treatments. However, used treatments had strong effects on the fruit characteristics and inner parameters, such as fruit diameter and Brix value. Moreover, it was established that the applied organic fertilizers increased the yield significantly.