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Qualitative detection of genetically modified organisms in plant samples
309-313Views:93We analysed the GMO content of corn samples by polymerase chain reaction following the appropriate optimization of the reaction. The analysis included two main steps: extraction of DNA from the sample, and detection of the GMO content by polymerase chain reaction. The polymerase chain reaction is an in vitro method to multiply chromosomatic or cloned DNA (cDNA) sequences through the enzymatic pathway. The reaction is sensitive enough to produce DNA in sufficient amount for the analysis from a single DNA. We identified the PCR products by agarose gel electrophoresis. When optimizing the reaction, the MgCl2 concentration, reaction time and temperature have to be taken into consideration. The temperature of the anellation has to be increased until the highest specificity and yield is reached. If the temperature of the anellation is too high, the primer is linked to non-specific sites as well; in the gel visualization, more lines can be seen at one sample. If the temperature of the anellation is too high, the primer is insufficiently linked or is not linked at all (too few lines in the gel visualization). After optimization, the GMO content in the unknown sample can be determined along with the appropriate positive and negative controls.
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Estimation of the vine-shoot yield in the Gyöngyös district
21-26Views:108Every year, a significant amount of vineyard prunings is generated in Hungary. Instead of utilization them, it is burned directly on the field in spite of the limitations of legalization highly pollute the environment in recent decades. The vineyard pruning itself or with other by-products from pomology and forestry can be significant amount of fuel source. However, before the planning to utilization the essential task is to estimate the potential. This study examine that how many vineyard is generated in the micro-region of Gyöngyös in every year on appointed vineyard.
In order to vine-branch potential calculate more accurate data was necessary so the products had to be determined for all plant. Thus, the biomass resource and the utilization become plannable. As the result of the experiment we can estimate that 1,5 t/ha vineyard pruning are generated. It means more than 9 thousand tons pruning in the micro-region of Gyöngyös. This amount can be convert into fuel source more than 150 thousand GJ heating value.
The results evince that the vineyard pruning is important fuel source in every year in the micro-region of Gyöngyös. Further usage is suggested for other regions in other size.
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Effects of the development of production factors on productivity
13-18Views:119In every economic sector, the enacted and expected quality of the products strongly affects its market entry and subsistence. The aim of management is success, competitiveness and subsistence that require adequate human and material resources and enough production stock. We must also notice that the quality of tools and instruments significantly influences work productivity. This applies to both plant production and animal husbandry.
The author researched milk production factors with methodical observation, document analysis and interviews. Data were collected with a ten-year interval, thus this assay can highlight the field, volume and sources of investments during this period and that the production stock increased in most farms and decreased in few. The data also shows the influence of investments on work productivity - there are differences between farms of equal size, so there are still ways of development.
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Agronomical and economic evaluation of different soil cultivation systems
47-52Views:58In the interest of profitable plant production and environmental protection, we have to make an effort to protect and improve the productivity of our soils while moderating production limiting factors. Due to different soil cultivation methods, the quantity of yield and required expenses also differ.
We examined the production costs in four different production technology systems. Overall, it can be said that farming standards are good, since cost prices were low (2001: 14-15 HUF/kg, 2002:15-21 HUF/kg, 2003: 39-49 HUF/kg) in the case of all main products per 1 kg. Cost prices were lowest in the case of direct sowing, probably due to low machinery costs.
All economic indicators have to be compared when choosing the most suitable production technology in a specific farming environment. -
Agronomical and Economic Evaluation of Different Soil Cultivation Systems
17-22Views:72In the interest of profitable plant production and environmental protection we have to make an effort to protect and improve the productivity of our soils while moderating production limiting factors. Due to different soil cultivation methods, the quantity of yield and required expenses also differ.
We examined the production costs in four different production technology systems. Overall, it can be said that farming standards are good, since cost prices were low (2001: 14-15 HUF/kg, 2002: 15-21 HUF/kg, 2003: 39-49 HUF/kg) in the case of all main products per 1 kg. Cost prices were lowest in the case of direct sowing, probably due to low machinery costs.
All economic indicators have to be compared when choosing the most suitable production technology in a specific farming environment. -
What does precision crop production hold for the future of soil science and plant nutrition?
411-421Views:206The concept of precision agriculture is straightforward at the scientific level but even basic goals are blurred at the level of everyday practice in the Hungarian crop production despite the fact that several elements of the new technology have already been applied. The industrial and the service sectors offer many products and services to the farmers but crop producers do not get enough support to choose between different alternatives. Agricultural higher education must deliver this support directly to the farmers and via the released young graduates. The price of agricultural land must be higher if well-organized data underpin the production potential of the fields. Accumulated database is a form of capital. It must be owned by the farmers but in a data-driven economy its sharing will generate value for both farmers and the society as a whole.
We present a methodological approach in which simple models were applied to predict yield by using only those yield data which spatially coincide with the soil data and the remaining yield data and the models were used to test different sampling and interpolation approaches commonly applied in precision agriculture. Three strategies for composite sample collection and three interpolation methods were compared. Multiple regression models were developed to predict yields. R2 values were used to select among the applied methods.
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Weed control possibilities of „energy willow” (Salix viminalis L.)
108-112Views:108In Spring 2005, the owner of the Szalka-Pig Ltd. of Mateszalka, decided to import the willow species Salix viminalis L. and to create a plantation for energy production purposes on the humid arable lands in his ownership. In 2006, he enlarged this area by 43 hectares. The owner further decided not only to plant this species on the more adherent (KA 70), but also onto the incoherent structured sand (KA 30-35). His main argument was that the cost of coal tar derivatives as energy sources was rapidly increasing in Hungary, so he needed to find a cheaper energy source for the drying of his products and for the heating of his buildings. He also planned to change his gas and oil heating equipment.
The willow’s cropping technology is being established in our country. One of our tasks is to work out an adequate weed control plan. The professional and safe use of herbicides can increase the success of production. In our paper, we discuss the data collected on treatments applied in the pre-emergent stage. The applied herbicide combinations (terbutilazin+S-metolaklór, mezotrion+ S-metolaklór, pendimetalin+S-metolaklór, oxyfluorfen+ S-metolaklór) yielded good results in large scale experiments. -
The role and impact of N-Lock (N-stabilizer) to the utilization of N in the main arable crops
51-55Views:214The nitrogen stabilizer called N-Lock can be used primarily with solid and liquid urea, UAN and other liquid nitrogen, slurry and manure. In corn it can be applied incorporated before sowing or with row-cultivator or applied with postemergent timing in tank-mix. In postemergent timing need precipitation for long effect. In oil seed rape and autumn cereals the N-Lock should be applied with liquid nitrogen in tank mix late winter or early spring (February-March). The dose rate is 2.5 l/ha. N-Lock increases the yield of maize, winter oil seed rape, winter wheat and winter barley 5-20 %. The yield increasing can be given the thousand grain weight. In case of high doses of nitrogen it can be observed higher yield. The quality parameter also improved, especially the oil content of winter oil seed rape and protein and gluten contents of winter wheat. The use of N-Lock increases the nitrogen retention of soil and reduces nitrate leaching towards the groundwater and the greenhouse effect gas emissions into the atmosphere. The degradation of the applied nitrogen is slowing down and the plant can uptake more nitrogen in long period. The effect of N-Lock the nitrogen is located in the upper soil layer of 0-30 cm and increasing the ammonium nitrogen form. The product can be mixed with herbicide products in main arable crops.
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The possibilities of biomass utilization in the field of spatial development
59-63Views:63By joining the European Union, Hungary made a commitment to increasingly utilize renewable energy sources. Keeping in view the agricultural circumstances of Hungary, we can state that biomass utilization has, overall, the greatest range of possibilities to use its products as ‘energy sources’. Biomass raw materials are useful in meeting emission control regulations for environment protection and to reduce climate change. The role of biomass production in spatial and rural development and in rural employment, and also in the decrease of Hungary’s energy dependence, supports development in biomass energy use. My aim in studying biomass utilization is the creation of a micro-regional level, decentralised, agro-energetic system-model for the use of plant and animal biomass for energy purposes, whether naturally or by anthropogenic activity, on agro- and sylvicultural areas, and on animal breeding farms. This model simulation method serves as a planning base for policy-makers during the spatial planning processes. The examination is presently in the stage of data collection. The collection of the basic data of the examination area and the purchase of the necessary equipment, materials and maps has already started. Contact has been made with local stakeholders, municipalities, municipality associations and micro-region managers.
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Examination the effects of different herbicides on the soil microorganisms of a calcareous chernozem
121-126Views:74Pesticides play a key role in fighting weeds, pests and parasitic fungi. According to surveys, pests reduce the yield of agricultural crops by 35% worldwide. Pests, fungi and weeds account for 14%, 12% and 9% yield loss, respectively (Gáborjányi et al., 1995). Chemicals have contributed to increasing and maintaining the yields of crop production for decades. Today, agricultural production (in spite of many efforts) is unthinkable without the use of pesticides (herbicides, insecticides and fungicides). On the other hand, these chemicals contribute to the pollution of the atmosphere, surface and underground waters, and agricultural soils, especially if they are applied improperly.
The sustainable agricultural production pays attention to environment-friendly cultivation-technologies; but at the same time it makes an effort to produce good quality and economical products. The examination of the herbicides’ secondary effects, fits into this chain of idas namely, how the herbicides affect – stimulating or inhibiting – the soil microbiological processes, prevention of soil fertility.
In the course of the experimental work the effect of herbicides on soil biological properties were examined in different maize (Zea mays) cultures. We wanted wished to know that how the herbicides affect the quantity change of soil microorganisms, the life of different physiological groups of bacteria and the activity of microorganisms. A small pot experiment was set up in 2008 with the application of two herbicides - Acenit A 880 EC and Merlin 480 SC – in the breeding house of the Department. The moisture content and nutrient supply were at optimal level in the experiment.
On the basis of results the following can be stated:
1. It can be stated that the two herbicides and all their doses affected negatively the number of total soil bacteria, the
inhibiting effects were significant. The quantity of microscopical fungi increased by the effect of Merlin 480 SC and decreased in the treatments of Acenit A 880 EC.
2. The Acenit A 880 EC had stimulating effect on the nitrate mobilization. The CO2-production was stimulated by the basic doses of herbicides; the other treatments did not influence the CO2-production significantly.
3. The quantity of microbial biomass-carbon –except for only one treatment- decreased significantly by the effect of herbicides. Besides it, the quantity of microbial biomass-nitrogen increased significantly in the treatments of Acenit A 880 EC.
4. The biomass of test plant decreased in the treatments of herbicides, their quantities were smaller than in the control. In the pots treated by Merlin 480 SC, parallel with the increase of doses decreased the quantity of plant-biomass. -
Evaluation of sweet sorghum and sudangrass varieties by the viewpoint of bioethanol production
57-61Views:122Bioenergy and biofuels are very important in today’s energy policy. These kinds of energy resources have several advantages against fossil fuels. Environmental protection is a cardinal point of widespreading these technologies but the economic considerations are important as well. In order to improve the rate of the renewable energy in the energy consumption, the European Union settled down a program which determines a minimum ratio of renewable energy in the energy consumption for each member country of the EU. To fulfil the requirements bioenergy and biofuels should be produced. This production procedure needs adequate stocks which are commonly agricultural products.
One of the promising stocks is sorghum. This plant fits for bioethanol production due to its juice content being rich in sugar. In this study six sweet sorghum hybrids, two sudangrass hybrids and a sudangrass variety have been evaluated to determine their theorical ethanol production capacity.
On the score of the results of the year 2009 it can be set that sudangrasses have a lower theorical ethanol capacity than sweet sorghums have. In the case of sweet sorghums 1860.29–2615.47 l ha-1 ethanol yields had been calculated, while the sudangrasses had only 622.96–801.03 l ha-1. After that throughout three years (2011–2013) the sweet sorghum hybrids have been evaluated in order to determine the fluctuations of the ethanol production capacity caused by the impact of the years. As a result 2425.44–4043.6 l ha-1 theorical ethanol capacities have been calculated, which means that sweet sorghums can be an adequate stock to produce bioethanol.