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Heterobeltiosis and combining ability among Egyptian cotton genotypes under well irrigated and water deficit conditions
5-18Views:336Two field experiments were carried out during the 2014 and 2015 seasons to evaluate certain genotypes of Egyptian cotton under well irrigated and water stress conditions. The cross combination Giza 69 x Australy recorded the best values for better parent heterosis for all physiological measurements and earliness index under well irrigated and deficit conditions. Of the female parents, Giza 86 was found to be a good general combiner for most yield characters under both normal and stress conditions, followed by Giza 94. Data illustrated that Giza 45 was the best general combiner for most fiber quality traits under both conditions. The cross combinations Giza 86 x Dandra and Giza 69 x Pima S6 showed significant positive SCA values for seed cotton and lint yield/plant under well irrigated and water deficit conditions. However, the combinations Giza 77 x PimaS6 and Giza 94 x 10229 recorded significant positive SCA effects for seed cotton yield/plant under stress. Whilst, the cross Giza 68 x 10229 recorded significant desirable SCA effects for most fiber quality properties. In this context, the estimates significant positive general and specific combining ability effects indicated that the epistasis and/or dominance effects for F1 hybrid in cotton could be important to a certain extent.
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Application of AFLP-Method in Plant Sample Identification
207-213Views:350One possible method for the determination of DNA-polymorphism is the PCR-based AFLP (Amplified Fragment Length Polymorphism). This method had been succesfully introduced to the Department of Botany at University of Debrecen in 2000-2001 with the examination of hay saffron (Crocus sativus L.) and its allies. Hay saffron is grown as a spice for some thousand years producing the most expensive spice in the world. This plant is sterile, triploid reproduces only vegetatively with no fertile seeds. However its origin is unknown it exists only in cultivation and it is a mutated variety of another species or an artificial or natural hybrid. Usual methods for the systematic examination are restricted hence it seemed to be reasonable to apply molecular biological methods in its case. Results of this work include the introduction and many fold application of the method beside ensuring the consequences of science literature with determining the C. cartwrightianus to give the most similar genetical pattern to C. sativus.
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The effect of water supply and crop year on the yield potential of sweet maize (Zea mays L. convar. saccharata Koern.) hybrids with different genotypes
203-210Views:384The successfulness of crop production is significantly affected by not only the the average yields that provide cost effectiveness, but also the success of striving for yield safety, therefore, varieties and hybrids tolerant to environmental stress factors are worth being included into the sowing structure. Our aim was to further the decision making of producers in prepaering the right sowing structure by the evaluation of sweet maize hybrids’ tolerance to excess rainfall.
We performed our examinations in an extremely wet year (2010) on chernozem soil on three sweet maize hybrids (GSS 8529, GSS 1477, Overland) in 12 replications. Comparing the yields of 2010 with those that can be expected under optimal rainfall conditions, we showed that the examined hybrids react to the amount of rainfall higher than their needs with yield depression. The excess rainfall tolerance of the examined hybrids is different in the case of each hybrid. -
Impact of environmental changes resulting from different sowing dates on maize yield
99-104Views:337Three Debrecen maize hybrids of different genotypes (Debreceni 285, Debreceni 377 and Debreceni 382) were examined on chernozem soil in a field experiment. During the two years of the experiment (2009–2010), we wanted to get to know how the examined hybrids reach to different sowing dates and what impact early, optimal and late sowing has on yield.
In 2009, balanced soil and air temperature resulted in steady emergence. However, the low temperature in early April and the cooling down in mid-May 2010 caused a delayed emergence.
The grain moisture content at harvesting and the high yield showed a strong crop year effect. In 2010, yield was much lower (1.664 t ha-1) and grain moisture was significantly higher (34%)than in 2009.
In 2009, early sowing resulted in yield decrease (P<0.05), but it also significantly reduced grain moisture at harvesting (P<0.05). Although late sowing slightly increased yield (not significantly), but grain moisture at harvesting increased by 9.2%. In 2010, optimal sowing date was shown to be the best alternative from the aspect of yield, but there was no significant difference in comparison with early and late sowing. Grain moisture at harvesting greatly increased (13.3%).
The Debreceni 382 maize hybrid reacted to sowing dates flexibly, neither early, nor late sowing affected its yield significantly and the grain moisture at harvesting showed 12% increase in the case of the late sowing date. In 2009, maize hybrids Debreceni 285 and Debreceni 377 reached their highest yield in the case of the sowing date which was shown to be optimal (23rd April), while the different sowing dates had no effect on yield in 2010.
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Effect of different N doses on maize yield and quality
97-101Views:408The effect of N doses on the yield and nutritional values of the Sushi (FAO 340) maize hybrid were analysed in three years (2018, 2019, and 2020). The analyses were performed at the Látókép Experimental Station of the University of Debrecen on calcareous chernozem soil, in a striped, small-plot, non-irrigated long-term field experiment. In the experiment, in addition to the non-fertilized treatment (A0), the N-fertilizer doses were applied as basic fertilizer and top dressing. The 60 and 120 kg N ha-1 dose (A60, A120) applied as spring basic fertilizer were followed by two phases of top-dressing in V6 (V690, V6120) and V12 (V12150, V12180) phenophases; the amounts were +30 and +30 kg N ha-1.
Maize yields were affected to varying degrees by crop year. The highest yields in 2018 and 2020 were recorded in the same V6150 treatment, while in 2019 the highest yield was obtained in the A120 treatment.
Increasing the N doses resulted in an increase in the protein content of the maize kernel. Depending on the fertilizer treatments and the crop year effect, the protein content of maize kernels varied between 6.2–10.2 g x 100 g-1. In all three years, the protein content was the lowest in the control treatment (A0) and the highest in the V6150 treatment.
The starch content ranged from 70.7 to 77.9 g x 100 g-1 in the average of the three years. In 2020, it was significantly higher in all nutrient treatments than in the other examined two years. The highest starch content - except for 2020 (A120, 77.9 g x 100 g-1) - was recorded in the A0 treatment (74.2, 72.3 g x 100 g-1).
The oil content of maize kernels varied between the values of 3.8 and 5.2 g x 100 g-1 in the average of three years. In terms of oil content, the results for 2018 and 2019 can be considered the same, while in 2020 it was significantly lower. Fertilizer treatments did not significantly affect the oil content of maize in any of the years.
The fertilizer dose applied in the V12 phenological phase was not effective in terms of yield and nutritional content (protein, starch and oil content).
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The effects of water supply on the physiological traits and yield of tomato
25-30Views:322The SPAD value, the chlorophyll fluorescence and the canopy temperature of the leaves and fruits of tomato hybrid “H1015” were investigated under non-irrigated (I0), deficit irrigated (I50) and well-irrigated (I100) conditions. The aim of the experiments was to show which treatment effect on the examined traits affected photosynthesis, leaf temperature and yield quantity, as well as quality under water scarcity. In the control treatment (I0), the canopy temperature increased, but the SPAD decreased compared to the other two treatments (I50 and I100). Chlorophyll fluorescence produced a fluctuating result. In the end, the number of the fruits was high, but the number of the sick and green berries was increased.
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Influence of H-FABP gene polymorphisms on slaughter value of hybrid pigs
55-60Views:605The H-FABP gene was defined as a potential candidate gene influencing the fat deposition traits, primarily the intramuscular fat content. The aim of this study is to define whether the previously reported gene mutations are connected with the slaughter traits measured in a standard slaughterhouse. The study included data from 405 gilts and barrows from 2 different samples. The two chosen mutation (HFABP1: c. 103 T>C, HFABP2: c. 1970 T>C) were detected in one reaction with PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Lenght Polymorphism) method with HinfI restrictoin enzyme. The allel frequencies are as follows: 103T(H)=0.75; 103C(h)=0.25, 1970T=0.32; 1970C=0.68. A HFABP1 mutation has significant effect on backfat thickness and lean meat % at stable 1 (sample 1), but there were no effect at stable 2 (sample 2). The analysis of values of production traits, depending on HFABP2 genotype did not reveal significant differences. Based on this study we can’t get a clear conclusion on the impact of polymorphisms on production parameters. In the examined flock the allele frequency of mutation in 5 'UTR is identical to the literature data, i. e. the more favorable variant regarding the intramuscular fat content is predominant in the population.
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Simplified volume equations for grey poplar (Populus × canescens Smith.) standing trees
27-31Views:318Grey poplar (Populus× canescens Smith.) is a natural hybrid of white poplar (Populus alba L.) and Eurasian aspen (Populus tremula L.). It can be found throughout Europe, where both parents are present. The above mentioned species of poplars (under the term of ‘domestic poplars’) cover approximately 5% of the forests of Hungary. Of these species, grey poplar holds significance in forestry, and its role in afforestation shows a growing tendency. For this reason, improving the growing technology of grey poplar is a timely topic. In this paper we introduce algorithms which help estimate grey poplar tree volumes without having to use volume tables. Based on the performed evaluations, both equations can be used for single tree volume estimation with an error of less than 5%.
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Test of the Plant Density Reaction of Genotype Sunflower Hybrids
113-119Views:534In order to produce sunflower in Hungary today it is important to develop hybrid-specific cropping technologies. The ever widening number of hybrids makes the constant examination of genotypes necessary from the viewpoint of genotype-environment interactions and critical elements. Plant density as a complex factor puts strain on the pathological features, yield and quality of sunflower. The experiment’s main objective is to find the optimal plant density for both the genotype and external factors.
As a result it can be stated that the optimal crop density is between 45,000-75,000 plant/ha. In 2001 the optimal density was 55,000 plant/ha. The Aréna PR and the Alexandra PR hybrids produced the greatest yields (3511 kgha-1; 3338 kgha-1). In the growing season of 2002, the yields were higher than in the previous year and the optimal crop density was 45,000-65,000 plant/ha. The best yields were produced by the Aréna PR and Alexandra PR hybrids in this year again (4102 kgha-1; 4267 kgha-1) and in 2003, 45,000-65,000 plant/ha proved to be the best crop density. The highest yield was produced by the Alexandra PR.
Analyzing the growing seasons of 2001, 2002 and 2003 it can be declared that as a result of dry climate of the three years yields were higher. It can be stated that the yield is decreased by higher than average of precipitation in the growing season. -
Micropropagation of Leuce-poplar clones and its role in selection breeding
43-48Views:370Leuce-poplars (mostly white poplar and its natural hybrid grey poplar) are native tree species trough Hungary. They are covering more than 4.0 per cent of the Hungarian forested area (77 000 ha). The white (grey) poplars play a significant role in the forestation under sandy soil site conditions as well as they are of importance from nature conservation point of view as well.Long-term selection breeding work is going on at the National Agricultural Research and Innovation Centre Forest Research Institute (NARIC FRI), involving selection of fast-growing Leuce-poplar clones under dry site conditions.Micropropagation technology is relatively quickly spreading in forestry. In vitro multiplication of trees is applied mainly in fruit growing in Hungary, in case of forestry it is used mainly for selection breeding.This paper presents a short overview on the micropropagation trials with different Leuce-poplar clones and the early evaluation of the seedlings growing of the micropropagated clones/varieties. -
Genetic progress in winter wheat quality and quantity parameters
71-75Views:407Wheat production is significant branch of Hungarian crop production (with about 1 million hectares of sowing area). Weather anomalies resulted by climate change have increased the importance of biological basis in wheat production. Yield quality and quantity parameters of three wheat genotypes sown on chernozem soil type after maize pre-crop were studied in a long-term field experiment. Yield amount of the studied genotypes varied between 2894 and 8074 kg ha-1 in 2017 and between 5795 and 9547 kg ha-1 in 2018 depending on the applied treatments. Based on our results it can be stated that in both studied crop years the highest yield increment was realized by the application of the nutrient supply level of N30+PK. As the result of the application of the optimum mineral fertilizer level – in contrast to the control – resulted in significant yield increment in both crop years. The results of the long-term field experiment prove that water utilization of the studied wheat varieties / hybrids was improved by the application of the optimal nutrient supply. Furthermore, the water utilization of the latest genotypes was more favorable by both the control and the optimum nutrient supply level treatments. Analyzing the quality parameters of winter wheat using the NIR method it has been stated that the quality results of the well-known genotype (GK Öthalom) were better than those of the new genotypes. A negative correlation between winter wheat quality and quantity parameters has also been confirmed. As the result of the mineral fertilizer application protein and gluten content of winter wheat increased to a significant extent.
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Comparative analysis on the fertiliser responses of Martonvásár maize hybrids in long-term experiments
111-117Views:232The results of experiments carried out in the Agricultural Research Institute of the Hungarian Academy of Sciences clearly show that in the case of hybrids grown in a monoculture greater fertiliser responses can be achieved with increasing rates of N fertiliser than in crop rotations. In the monoculture experiment the parameters investigated reached their maximum values at a rate of 240 kg/ha N fertiliser, with the exception of 1000-kernel mass and starch content. In both cases the starch content was highest in the untreated control, gradually declining as the N rates increased. Among the parameters recorded in the crop rotation, the values of the dry grain yield, the 1000-kernel mass, the protein yield and the starch yield were greatest at the 160 kg/ha N fertiliser rate, exhibiting a decrease at 240 kg/ha. Maximum values for the protein content and SPAD index were recorded at the highest N rate. It is important to note, however, that although the N treatments caused significant differences compared to the untreated control, the differences between the N treatments were not significant.
In the given experimental year the values achieved for the untreated control in the crop rotation could only be achieved in the monoculture experiment at a fertiliser rate of 160 kg/ha N, indicating that N fertiliser rates could be reduced using a satisfactory crop sequence, which could be beneficial from the point of view of environmental pollution, crop protection and cost reduction.
The weather in 2006 was favourable for maize production, allowing comparative analysis to be made of the genetically determined traits of the hybrids. Among the three hybrids grown in the monoculture experiment, Maraton produced the best yield, giving maximum values of the parameters tested at a fertiliser rate of 240 kg/ha N. The poorest results were recorded for Mv 277, which could be attributed to the fact that the hybrid belongs to the FAO 200 maturity group, while the other hybrids had higher FAO numbers. Maraton also gave the highest yields in the crop rotation experiment at the 160 kg/ha N level. All three hybrids were found to make excellent use of the natural nutrient content of the soil.
It was proved that the protein content of maize hybrids can only be slightly improved by N fertilisation, as this trait is genetically coded, while the starch content depends to the greatest extent on the ecological factors experienced during the growing season. -
Evaluation of dry matter accumulation of maize (Zea mays L.) hybrids
35-41Views:1019The increase of the grain yield of maize is closely correlated with its seasonal dry matter accumulation. Dry matter is accumulated into the grain yield during the grain filling period. The following maize hybrids were involved in the experiment: Armagnac FAO 490, Loupiac FAO 380 and Sushi FAO 340. In order to determine dry matter content, two samples per week were taken on the following days: 22nd, 25th, 28th, 31st August, 4th, 7th, 14th, 18th, 22nd, 25th, 29th September and 2nd, 6th, 9th, 13th October. In the course of sampling the weight of 100 grains from the middle section of 4 ears was measured in 4 replications. Dry matter content was determined after drying to constant weight in a drying cabinet at 60 °C. Harvesting was performed on 13th October 2017.
The daily precipitation sum was determined by local measurements, while the daily radiation and temperature data were provided by the Meteorological Observatory Debrecen of the National Meteorological Service in Budapest. Among the agrometeorological parameters, an analysis was made of the precipitation during the growing season, effective heat sums during the vegetative and generative phase, and the water supplies. The daily heat sums were determined using the algorithm proposed.
The amount of precipitation in the winter period before the 2017 growing season was 210 mm. The soil was saturated until its field capacity. The rather dry and warm March and April had a favourable effect, but there was no worthy amount of precipitation until May (51 mm) due to the condition of the dried seedbed. Sowing was performed on the 5th of May 2017 in a randomised small plot experiment. There was favourable precipitation and temperature during the growing season, thereby providing ideal conditions for maize development, growth and yield formation. There was near average amount of precipitation in each year. The total amount of precipitation in the summer period is 342 mm. Temperature was mostly above the average, but there was no long and extremely warm period.
The Armagnac hybrid reached its highest dry matter mass 126 days after emergence. Physiological maturity was reached sooner (on the 119th day) in the case of Loupiac, and even sooner in the case of Sushi (116th day). The thousand grain weight of Sushi (which has the shortest ripening period) was 286 g at the time of physiological maturity, while that of Loupiac was 311 g. Compared to Sushi, Armagnac showed 12 g more dry matter accumulation (306 g). In the case of all three examined hybrids, physiological maturity was preceded by an intensive phase, when the dynamics of dry matter accumulation was rather quick. On average, Sushi gained 2.8 g dry matter per day between 103 days following emergence and physiological maturity, while the same values were 3.2 g for Armagnac and 3.3 g for Loupiac. The aim of the regression line slope is to predict the behavior of the dependent variable with the knowledge of the values and characteristics of the independent variables using the regression line equation. Furthermore, to determine how the location affected the dynamic of dry matter accumulation in the Armagnac, Loupiac and Sushi hybrids. In regression analysis, the coefficient of explanation showed that the effect of day in the Armagnac was 97%, in the Loupiac 94%, in the Sushi 90 %. The determination coefficient (R2) is useful in determing how the regression equation fits. But, as we have seen, the determination coefficient alone is not sufficient to verify the model’s accuracy, in addition to the determination coefficient (R2), the normality of the data or the residuals, the variance of the variables at different levels, the independence of the data relative to time and non-oblique. Observations are evaluated for the correctness of the fitted model.
Dry matter values decreased evenly and slightly following physiological maturity. According to our research results, it was established that physiological maturity is followed by a moderate dry matter loss. Until harvesting, Armagnac lost 40 g of its thousand mass weight in 29 days, while the same value pairs were 69 g in 36 days for Loupiac and 29 g in 39 days for Sushi. Loupiac – which had the highest weight at the time of physiological maturity – lost the most of its dry weight; therefore, Armagnac and Sushi had higher values at the time of harvesting.
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Determining elements of variety-specific maize production technology
157-161Views:303Our aim was to work out such new maize fertilizer methods and models which can reduce the harmful effects of fertilization, can
maintain the soil fertility and can moderate the yield fluctuation (nowadays 50-60 %).
The soil of our experimental projects was meadow soil. The soil could be characterized by high clay content and pour phosphorus and
medium potassium contents. In the last decade, out of ten years six years were dry and hot in our region. So the importance of crop-rotation
is increasing and we have to strive for using the appropriate crop rotation.
The yields of maize in monoculture crop rotation decreased by 1-3 t ha-1 in each dry year during the experiment (1983, 1990, 1992,
1993, 1994, 1995, 1998, 2000, 2003, and 2007). The most favourable forecrop of maize was wheat, medium was the biculture crop rotation
and the worst crop rotation was the monoculture.
There is a strong correlation between the sowing time and the yield of maize hybrids, but this interactive effect can be modified by the
amount and distribution of precipitation in the vegetation period. At the early sowing time, the grain moistures were 5-12 % lower compared
to the late sowing time and 4-5 % lower compared to the optimum sowing treatment.
There are great differences among the plant density of different maize hybrids. There are hybrids sensitive to higher plant density and
there are hybrids with wide and narrow optimum plant densities.
The agro-ecological optimum fertilizer dosage of hybrids with a longer season (FAO 400-500) was N 30-40 kg ha-1 higher in favourable
years as compared to early hybrids.
We can summarize our results by saying that we have to use hybrid-specific technologies in maize production. In the future, we have to
increase the level of inputs and have to apply the best appropriate hybrids and with respect to the agroecologial conditions, we can better
utilize the genetic yield potential. -
The effect of season and fertilizer on the LAI, the photosynthesis and the yield of the maize hybrids with different genetic characteristics
27-34Views:365The experiment was carried out in Debrecen, at the Experimental Station of the University of Debrecen Centre of Agricultural Sciences, Department of Crop Production and Applied Ecology. We tested 10 various hybrids with their own genetic characteristics for five different fertilizer doses, in addition to the parcels without fertilization. The three factors of production technology jointly determine the successfully of maize production, but in different measure. The yield and the stability of yield of maize can be increased with hybrid-specific technologies.
In 2004-2005 experiment years the favorable results reached were due to the rainy season. There were significant difference between the productivity of maize hybrids. The N 40, P2O5 25, K2O 30 kg/ha treatment caused the highest increase of yield (3-5 t/ha) compared to the control (parcels without fertilization). The reaction of hybrids to the further fertilizer doses was different. The agro-ecological optimum of NPK fertilization was N 120, P 75, K 90 kg of the most hybrids.
During the experiment, we tested the moisture loss of the five hybrids. The seed moisture content at harvest was higher than in previous years due to the rainy seasons. The seed moisture content of harvest of FAO 200-300 hybrids were about 20%. It changed between 21-24% in the case of hybrids with longer vegetation period (FAO 400), the seed moisture content of Mv Vilma (FAO 510) was 24.21-25.04% in the average of fertilizer treatments. There is an important difference between the moisture loss ability of hybrids which changed 0.2-0.6%/day. The moisture loss of hybrids changed depending on the fertilizer treatment; usually, it was more favorable in the optimal fertilizer dose (N120+PK).
In the case of tested hybrids, we measured the highest LAI and photosynthetic activity at the optimal treatment, N 120, P2O5 75, K2O 90 kg/ha in the respect of efficiency and environmental protection, and the yield was high also for this treatment. There are significant difference between the LAI, the photosynthetic activity and the yield of hybrids, and these values could change depending on the treatment of fertilization. -
Development of an InDel marker set to establish hybridization between wild boar and domestic pig (Sus scrofa) breeds
21-25Views:586Wild boar and domestic pig breeds belong to the same species (Sus scrofa), so they can easily have viable offspring. This could be a problem in preserving the genetic lines of wild boars, keeping clean the food industry from lower-grade hybrid boar meat, and „producing” ethically questionable trophies, too. The aim of our study was to develop a cost-efficient, fast, easy and accurate marker set which can separate the wild boars from hybrids and domestic pig breeds.
The InDel markers were developed using 59 full pig genomes of 17 different breeds (e.g. Duroc, Large White, Landrace, Mangalica, wild boar). Sequence differences between the genomes of wild boars and domestic breeds were identified in variant call files, and verified using the IGV software. Wild boar, mangalica and duroc specific primers to amplify the chosen InDel regions were designed using Primer3.
After preliminary tests five markers were chosen, three wild boar specific, one Mangalica specific and one Duroc specific one. Fluorescently labelled primers were used to make the valuation easier and more accurate with capillary electrophoresis instead of gel-electrophoresis. The markers were optimised individually and in multiplex conditions and tested in samples of 11 breeds.
In conclusion, a new, faster and cheaper set was developed to separate the wild boars from the hybrids and domestic breeds. Based on the preliminary testing on wild boars, duroc and mangalica breeds zero samples resulted false negative, so it is 100% accurate. In addition, it is a much more cost- and time-effective way than testing every single sample with STR sets.
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Production of corn in Serbia in the light of climate change
305-322Views:346This paper presents the basic elements of maize production technology in Serbia. For their good knowledge, it is necessary to be familiar with the basic biological requirements of the species, phenology and its particular requirements for water, heat, soil conditions and mineral nutrition. A special emphasis in this paper is given to recent research by the author regarding the correction of production technology (selection of hybrids, time and density of sowing, etc.) from the aspect of knowing the weather conditions of the year to the moment of sowing and the amount and position of available nitrogen in the soil profile during the spring before sowing. In the light of predicted climate change conditions for the Republic of Serbia, the authors of this paper have proposed and appropriated adaptation measures. The general conclusion is that there are no general recipes for the correct production technology of this plant species, but should be adapted to each specific year, field and hybrid.
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The Effect of Tillage Treatments on Soil Temperature at Planting and on Corn (Zea mays L.) Yield
40-44Views:511The effect of soil temperature was evaluated on the yield of the Occitan corn hybrid at a depth of 5 cm. We examined this effect on the time required from planting to emergence for three average durations: five, ten and fifteen days, all calculated from the day of planting. Winter plowing (27 cm), spring plowing (23 cm), disc-till (12 cm) treatments and 120 kg N per hectare fertilizer were applied. As a result of our analysis, we determined the post planting optimum soil temperatures for various time periods. The average soil temperature for a time period of 15 days post planting is the most usable for determining actual yields, followed by ten days, with five days proved to be the least usable (winter plow R2 = 0.86, spring plow R2 = 0.87, disc-till R2 = 0.64).