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  • Examinations of the carbon dioxide emission of the soil in the case of different tillage methods in a field experiment
    209-212
    Views:
    593
    Today's global challenge is the increasing concentration of carbon dioxide (CO2) and other greenhouse gases in the air. The level of CO2 emissions may be significantly affected by the agriculture and, more specifically, the applied tillage method, even though to a lesser extent than industrial production. On a global scale, the CO2 emission of an agricultural area is insignificant in comparison to that of a large-scale plant in an area of the same size, but areas under cultivation, including arable land, have a large global area. In this paper, we investigated the relationship between applied soil tillage methods and carbon dioxide emissions in the case of different fertiliser treatments. In our experiment we examined four types of tillage with five different fertiliser effects. Comparing fertiliser treatments and tillage methods, it was found that their interaction significantly affected carbon dioxide emissions, the lowest value was obtained in the case of the 210 l ha-1 Nitrosol+N-LOCK – tillage radish treatment. Strip and tillage radish methods have relatively homogeneous, low value.
  • Effect of Moringa leaf extract and set size on the bulb weight, diameter and yield of onions (Allium cepa L.)
    127-131
    Views:
    719

     Studying the use of natural extracts or biostimulants in improving vegetable plants is the current needs as an alternative way to the use of chemical products. This experiment was carried out to evaluate the effect of Moringa oleifera leaf extract (MLE) as a natural growth regulator on bulb diameter, weight and yield of onions ‘Texas Early White’ cultivar. Therefore, three concentrations (control, 2%, 4% and 6%) of MLE and three different onion set sizes [small (3±1 g/set), medium (6±1 g/set), and large (10±1 g/set)] were prepared in a randomized complete block design with three replications and plant spacing 20×15 cm. MLE was applied as a foliar spray directly onto the plants with the amount of 25 ml/plant every two weeks from sprouting to maturity. The achieved results indicate that the interaction of foliar spray with moringa leaf extract 4% and large set size of 10±1 g/set gave the highest value of the total yield 4802.7 g m-2, while the lowest yield 1531 g m-2 was recorded in the interaction of control and the smallest set size of 3±1 g/set. Also, a significant difference was recorded in bulb diameter separately. Thus, the largest bulb diameter 68.90 mm was obtained from the planting of the largest set size 10±1 g/set with 4% of MLE while the smallest bulb diameter 38.40 mm was recorded from the smallest set size 3 ±1 g/set with control treatment. Similarly, a significant effect was recorded in both set size and MLE separately. The highest bulb diameter 58.71 mm was achieved in the planting of the largest sets 10±1 g/set, while the lowest diameter was 42.12 mm in the planting of the smallest set size 3 ±1 g/set. Similar results were recorded for the bulb weight and total yield. The concentration of 4% MLE produced the highest bulb weight 99.74 g/bulb and yield 3324.5 g m-2, while the lowest bulb weight and yield was obtained in the control treatment 55.61 g/bulb, and 1869.3 g m-2. Consequently, the results indicate the positive effect of Moringa leaf extract which can be applied as a natural substance in the form of foliar spray at critical growth stages to improve the growth and yield of dry onions.

  • Distribution of family farms according to estate size and land usage in Hajdú-Bihar County
    130-136
    Views:
    189

    In harmony with European tendencies, the role of agriculture and its share in GDP output, as well as in employment, is continuously decreasing in Hungary and Hajdú-Bihar County. At the same time, according to the specialized literature, the role of agriculture is still extremely important in the income of the rural population and in easing the present social tensions, and this will not change in the future. The economic and social processes of the last one and a half decades caused radical changes in agriculture. The above-mentioned processes resulted in new property and organizational structure in the field of leasehold and land structure. The rational land concentration which came to pass in the last few years can be mentioned as a favourable tendency that improves the efficiency of agricultural activities, as well as the more effective land usage accompanied by this process. In addition, it supports the integration with principles formulated in the Common Agricultural Policy. In this study, I survey the effects of established processes and the change of land usage in the case of individual family enterprises in Hajdú-Bihar County. The choice of the examination area was motivated by the higher proportion of agricultural area in comparison with the national average and the fact that this sector has great importance today, too.

  • Definition antioxidant activity of selenium-enriched food sprout, as well as their microbiological analysis
    25-30
    Views:
    606

    In this present study, we prepared selenium-enriched food sprouts, where the antioxidant capacity was analysed, we also determined their microbiological status. We took into account the fact, we choose micronutrients to our treatment, that selenium can be delivered to the body by a small amount with the most widely consumed food.

    We focused during our research to determine that the increasing concentrations of selenium treatment, in which we used sprouts, knowing fully well that it has an impact on aboriginal antioxidant capacity of sprouts, which is mainly due to high vitamin content of sprouts.

    Furthermore, we think it is important to make microbiological analysis, because germination conditions, for example temperature, pH, all this will create an ideal environment for the growth of microorganisms. So we had goal to determine, how the used selenium concentration affect the total plate count, coliform bacteria count and Staphylococcus aureus count of sprouts.

    We determined the aboriginal water-soluble and lipid-soluble antioxidant capacity of sprout with the PHOTOCHEM chemiluminometer and we applied pour plate technique for the mapping of the mycrobiological state of sprouts.

    Experimental results are evaluated, we state that increasing concentrations of selenite or selenate treatment, is primarily water-soluble antioxidant capacity of sprouts was affected. The water-soluble antioxidant capacity of wheat sprout was much higher than the measured values in pea sprout, this may be linked to what we measured. That is much higher ascorbic acid content in case of wheat sprout, which is well known as one of the most important antioxidant properties compounds of wheat sprout.

    We conclude from the results of the microbiological, that the highest concentrations of selenite or selenate treatment has a relative significant anti-microbial effect in case of wheat sprouts. Coliform and total plate count showed no clear decreasing tendency, although the values of treatments in both cases obtained were below the control values.

  • The Target Areas of Regional Development, Tool and Institutional Background, its Present and Future Prospects
    138-142
    Views:
    291

    Regional politics determines the aims of regional structures, and the tools with the institutional background on the whole necessary achieve these. Realizing the aims of regional politics is a precondition of executing the basic principles of regional development and politics. The regional institutional system for handling the decentralized financial resources has not evolved in Hungary yet. The professional institutes for preparing the programs have not been set up, and the management of execution is slowly taking shape. There are still deficiencies in the domestic application of concentration and addition principles. A number of funds are provided on a national level, that are directly or indirectly aimed at regional development. The obvious coordination among the funds is not carried out neither on a national, nor on a regional level, partly to avoid parallel consumption aims. The future of Hungarian spatial structure depends on the type of decentralization strategy the country will follow for the new resources after the accession. Total decentralization offers the most efficient solution for Hungary.

  • Changes in the herbicide tolerance of maize genotypes in wet and dry years
    124-127
    Views:
    248

    The tolerance of 15 inbred maize lines grown on chernozem soil with forest residues in Martonvásár was tested against herbicides applied post-emergence in two dry, warm years (2003 and 2011) and in two cool, wet years (2004 and 2010). The herbicides mesotrione + terbutylazine, nicosulfuron and dicamba were applied to maize inbred lines in the 7–8-leaf stage at the maximum dose authorised for practical use and at double this rate. The plants were scored for the intensity of visible phytotoxic symptoms 14 days after treatment.
    The level of phytotoxicity observed in dry, warm years was 5.14%, averaged over the lines, herbicides and rates. The intensity of visible symptoms was almost 2.5 times as great in cool, wet years (12.76 %).
    Averaged over the four years, the lines and the rates, the least damage was caused by dicamba (5.77 %), followed by mesotrione + terbutylazine (7.23 %). The most severe symptoms were induced by nicosulfuron (16.17 %). This could be attributed to the fact that some of the inbred lines were extremely sensitive to herbicides, especially those of the sulfonylurea type.
    A difference of more than 1.5 times was observed between the two doses, but the correlation between the concentration and the severity of the visual symptoms was not strictly linear. Compared to the normal dose (100 %) the double rate resulted in a 162.5% increase in symptom severity. In most cases plants treated with the normal dose were symptom-free or only exhibited a low level of phytotoxicity.

  • Effect of genotype on the hematological parameter of TETRA-SL and Hungarian Partridge coloured chickens at young age
    99-104
    Views:
    599

    Indigenous chickens are well known for their dual-purpose function and palatable meat. However, the information on their hematology parameters is lacking and hence hampering the poultry industry production of local breeds. The main objective of this study was to examine the hematological parameters of Hungarian Partridge Coloured hen (HPC) and TETRA-SL (TSL) genotype and determine the hematological reference interval values. This trial was part of the larger experiment where a total of 200 chicks (HPC + TSL) were reared. For this trial the blood samples were obtained from brachial wing veins of 8 chicks of each genotype for blood hematological analysis. The results indicated that some of the hematological parameters such as red blood cells-RBC, white blood cells-WBC, hemoglobin-Hb, hematocrit-Ht, platelets-Plt, lymphocytes-LYM, and granulocytes-GRAN were significantly affected by the genotypes (p < 0.05). The genotype did not affect the mean corpuscular volume-MCV, mean corpuscular hemoglobin-MCH, mean corpuscular hemoglobin concentration-MCHC, and GRAN (p > 0.05). The hematological reference interval values were slightly higher in the TSL genotype compared to HPC chicks. It is concluded that genotype has a significant effect on the hematological parameters. The results from this trial will be help and design the baseline reference values for the HPC genotype which will be useful in assessing the health status of these indigenous chickens.

  • Study of The Amount of Produced Animal Manure and Biogas Potential in a 5, 20 and 40 km Zone around Debrecen
    64-68
    Views:
    166

    The guiding principle for our research is that decentralized energy supply systems should be located near local consumers. One big, local market for energy from biogas plants in Hajdú-Bihar county is Debrecen. In the course of the investigation of biomass suitable for biogas production in the area of Debrecen, this study determines the quantity of biogas producible from animal manure. Municipalities around Debrecen were examined in circles with 40, 20 and 5 km radius. Livestock data of these settlements stem from the Agricultural Census in year 2000, actualized by the county trend of livestock changes. Manure quantity and biogas potential in these places are estimated with coefficients from the literature and our calculations. In the largest circle, about 1.4 million tons of manure is produced, from which round 94 million m3 biogas can be produced. Biomass produced in the settlements and producible biogas shows a remarkable trend of concentration, which supports our recommendation on the system of energy supply.

  • Az évszak és az azonos évszakban történt ismételt mintavételek hatása holstein tenyészbikák ejakulátumának minőségi- és mennyiségi jellemzőire
    33-37
    Views:
    247

    This study was carried out at the Hungarian National Artificial Insemination Centre in order to evaluate the seasonal effects on the quantitative and qualitative parameters of sperm production using sperm analyser equipment. The volume of ejaculate (VE), percentage of living sperm (PLS), spermatozoa concentration (SC), motility score (MC), percentage of motile spermatozoa (PMS), speed of movement of sperm cells (SMS) and the percentage of sperm cells moving straightforward (PSMS) were collected and observed in three seasons (summer, autumn, winter) in Holstein-Friesian breeding bulls (n = 15). The sperm collection was done for three successive weeks in every season on the same day (summer: n = 41, autumn: n=39, winter: n = 42, altogether: n = 122). The same sperm samples were measured by sperm analyser equipment (HTM version 7.0, Danvers) for PMS, SMS and PSMS. The seasonal and the number of sperm collection effects were confirmed on semen characteristics by multiple analysis of variance (two ways MANOVA, Type III) using the program package of STATISTICA 4.5. The average values of VE were similar in the three seasons (summer: from 4.42 to 6.28 cm3, autumn: from 4.08 to 6.86 cm3, winter: from 5.43 to 5.71 cm3). The average values of the MC were similar in each of the three seasons (summer: from 3.66-4.00 M; autumn: from 3.66-3.77 M; winter: from 3.86-4.07 M). The summary of all effects for season (P<0.001), repeated sperm collections in the same season (P<0.05) and interaction of two traits (P<0.01) were established on the measured characteristics. The special effects (P<0.05) of a given season were calculated, excepting the VE and MC, on all of the measured characteristics. The special effect (P<0.01) of the repeated sperm collections in the same season was verified in only one case (SMS). It is not surprised at the interaction was established on the VE (P<0.001), PMS (P<0.05), and SMS (P<10.0). The results of present study suggest that seasonal effect on sperm quality of breeding bulls cannot be eliminated even at standard feeding and keeping conditions. Considering our results, we can recommend that the average values of three successive weeks in every season be used, to take advantage of seasonal effect on the quantitative and qualitative parameters of sperm production.

  • Effect of soil-compost proportion on the abiotic and biotic parameters of soilplant system
    99-104
    Views:
    337

    The environmental awareness, coming to the front in the 21st century, motivates us to supply the plant nutrient demand (in point of the plant, the environment and the human health) with natural materials.
    Composting is known since the beginning of civilization. We came to know more the processes of composting as a result of last decades’ research, but numerous unexplained questions remained up to this day. The good compost is dark gray or brown, and it should not create an odor. It has aggregate structure, and it’s pH is neutral. Compost is soil-like (Fehér, 2001), nutrient-rich material, which contains valuable nutrients extracted from soil, so if we recycle this, we can decrease the chemical fertilizer and other (example: mineral energy) expenses.
    The reason of that we chose the more accurate cognition of compost utilization is to do more effective the site-specific nutrient supply. This increases the average yield and the quality of yield. Besides we can decrease the harmful effects, which endanger the plant, the environment, and the human body.
    During the compost utilization experiment we blended the  acid sandy soil with compost in 4 different volumetric proportions (5 treatments) than we set the pots randomized. The advantage of this method is that we can provide equal conditions for plants so we can measure the effect of  treatments correctly. Our experimental plant was ryegrass (Lolium perenne L.), that grows rapidly, tolerates the glasshouse conditions, and indicates the effect of treatments well. After the harvest of ryegrass we measured the fresh and dry weight of harvested leaves and the total C-, N-, S-content of the dry matter and of the soil, we examined the pH and the salt concentration of  soil as well. 
    Our aim was to study and evaluate the relations between the compost-soil proportion and the nutrient content of soil and plant. In our previous experiments we confirmed (based on variance analyses) that the compost has a beneficial effect on soil and increases the nutrient content of the soil (Szabó, 2009). But it’s important to appoint that the compound of compost is seasonally change: in winter the selective gathered municipal solid waste contains salt that were applied for non-skidding of roads, but salt has a negative effect to the plant. We proved that in our experiment the 25/75% compost/soil proportion was ideal for the plant. This content of compost effected 6 times higher green matter weight compared to the 100% sandy soil. 

  • Effect of arsenic treatments on physiological parameters of sunflower and maize plants
    81-84
    Views:
    679

    The environment is contaminated with heavy metals and other toxic compounds. One of the most important toxic element is the arsenic (As).

    The objective of our study was to investigate the effect of As on fresh and dry weight of sunflower and maize in the early growth phases.

    Seedlings were grown in climate room on nutrient solution which were treated with 3, 10 and 30 mg kg-1 arsenic. The plants were treated separately with As(III) and As(V). After 14 day, changes in fresh and dry weight of maize shoots and roots were recorded. In the case of sunflower these parameters were measured after 21 day.

    The applied As(III) and As(V) decreased the fresh and dry mass of the shoots and roots of seedlings, especially at concentration 30 mg kg-1. We can draw the conclusion that the treatments of the maize and sunflower roots with arsenic had negative effects on the biomass accumulation. We found that the sunflower plants are more sensitive to arsenic toxicity than maize plants, and all data demonstrate that the As(III) is more toxic to these plants than the As(V).

  • Effect of Selenium Supplementation on in vitro Radish and Green Pepper Seedlings Germination
    149-155
    Views:
    629

    Selenium (Se) is an essential trace element for animals, microorganisms and some other eukaryotes. It has become increasingly evident that Se plays a significant role in reducing the incidence of lung, colorectal and prostate cancer in humans. Although there is evidence that selenium is needed for the growth of algae, the question of essentiality of Se in vascular plants is unresolved. Therefore Raphanus sativus (Se accumulator) and Capsicum annuum (non Se accumulator) were treated with 0-200 mg/l natrium-selenate. The results showed that lower (2 mg/l) concentration natrium-selenate increased the fitomass and total antioxodant capacity in seedlings.

  • Agriculture production and rural development
    129-133
    Views:
    203

    In a national economy, the considerable part of its available territory consists of the so called rural area which is mainly used by the agricultural sector. In our days, the rural areas are characterized by the loss of their economic, administrative etc. functions. The agricultural sector is able to utilize the rural areas, though other economic activities play important role as well. To evaluate the agricultural sector in terms of the agribusiness, it is clear that the situation of the agricultural sector is not only decisive for the rural areas, but also for the whole national economy, and therefore, it is part of its balanced development.

  • The effect of irrigation on the yield and quality of maize (Zea mays L.) hybrids
    143-147
    Views:
    358

    In this study, the effect of water supply on the quality and productivity of different maize hybrids was observed.

    Maize production is influenced by many agro-technical factors such as nutrient supply, plant density, environmental factors, water supply and temperature. Good soil quality and adequate technology significantly reduce the unfavourable effect of crop year. The impact of fertilization, crop rotation, irrigation and plant density on maize yield was greatly affected by crop year and crop rotation. The main constituent (69–75% dry matter concentration) of maize seed is starch, however, its increase can be achieved only to a limited extent, as increasing the starch content will reduce other parameters, especially protein content. Significant nutrient content improvements can be achieved by appropriate hybrid-site connection and the use of adequate technology. Protein content reduction with optimal water supply can be positively influenced by the appropriate nutrient supply for the hybrid.

    The experiments were carried out at the Látókép Experimental Site of Crop Production on calcareous chernozem soil. The weather of the examined year, which was partly favorable for maize and partly unfavourable in other respects, was also reflected in the development of maize and yields achieved.

    In 2019, we were examining the hybrids of Kamaria (FAO 370), P 9903 (FAO 390), DKC 4351 (FAO 370) and KWS Kamparis (FAO 350–400). Due to the dry soil condition, sowing was delayed, however, the hybrids emerged ideally because rain arrived soon after sowing, which facilitated initial development. Young plants evolved rapidly and dynamically in the case of all hybrids. In terms of heat-demanding bread, the month of May was unfavourable to temperatures below the annual average, but the higher amount rainfall helped the development. However, due to the drier period in early July, 25–25 mm of irrigation water was applied to half of the experimental areas on July 1 and 15.

    The aim of our research was to determine the best population density for hybrids under favourable soil conditions (calcareous chernozem soil). In both irrigated and dry conditions, a crop density of 75,000 crops were the most favourable for the Kamaria hybrid. The P9903 hybrid in the case of a crop density of 85 crops proved to be ideal and the DKC4351 had an optimal population density of 95,000.

    However, it should be taken into consideration that, in the case of soils with poor water management, the drought sensitivity of the crop stand may increase at a population density of 95,000.

    As a result of irrigation, yield increased and the difference between the examined plant numbers decreased. The yield growth was relatively moderate (341 kg ha-1 – 1053 kg ha-1), which makes the economicalness of irrigation doubtful in the given year.

  • Coherence and connection between the good pond culture practice and the environment conscious management
    60-73
    Views:
    189

    According to the data indicating the decline and restructuring during the past decade, as well as the trend in the European Union member states, it can be expected that the role of traditional agriculture and fish production in direct rural employment decrease further. This also values those strategic directions for restructuring that will lead fishculture from quantity driven to quality production along with sustainable development (i.e. environmental conscious production) and multifunctional farming. This way the economic and social tensions caused by the concentration of the production and labour output can be mitigated.
    It is laid in the 1257/1999 Act on rural Development that farmers that enrol the agri-environmental scheme should follow the “Good Agricultural Practice” on the whole managed area. In case of agri-environmental schemes this is a precondition for which no grants are given.
    The adaptation of “Good Agricultural Practice” in fishproduction,where it is called: “Good Pond Culture Practice” is considered important on the basis of the above mentioned. This programme is undertaken in co-operation with the Research Institute for Aquaculture, Fisheries and Irrigation, University of Debrecen, Faculty of Agricultural Economics and Rural Development and the Association of Hungarian Fish Farmers and Product Council.
    The European Commission proposed the formulation of the European Fisheries and Aquaculture Fund (EFAF) for the period 2007-20013, which will replace the Financial Instruments for Fisheries Guidance (FIFG), but it also consists of several new elements and will be working differently, too. According to the proposal the budget for the Fund will be nearly 5 billion EUR (4963 million EUR). The development level of aquaculture and fisheries and the social and economic significance in the given member state will be considered when distributing the Fund between the Member States.
    According to the plan the Fund is organised along five priority axes, of which the most important for the Hungarian fisheries sector is No. II: Aquaculture and the processing and marketing of aquaculture and fisheries products. The main measure areas are the followings:
    1. investment support for aquaculture;
    2. support for aquatic-environmental schemes;
    3. environmental- and animal health issues;
    4. investments in processing and marketing
    In case of accessing support under measure area No. 2 farms are obliged to meet the requirements of the scheme beyond the “good management practice” for 5 years, which is to be supervised by the approved body of the Member State. For this reason our work is considered to be substantial.
    Approval of the application of “Good Pond Culture Practice” is based on two elements: first the prevailing environmental and nature conservation regulations, as well as the list of controllable conditions in the new agri-environmental agreements are to be met. “Good Pond Culture Practice” are to be conducted on the whole farm area. Its main elements are:
    - nutrition management,
    - feeding,
    - pond maintenance,
    - stocking,
    - harvesting,
    - animal welfare (storage and over-wintering).

  • Changes in the Sulphur Content of Winter Wheat in a Field Experiment
    85-88
    Views:
    201

    The use of superphosphate as P-containing fertiliser decreased in the last years in many countries in accordance with strict air pollution laws, and the S-deposition decreased from the atmosphere to the soil as well.
    Winter wheat is the one of the S-demanding plants. Recently, the gradually increasing S absence endanger the formation of required average yield of winter wheat, and has bad effect on its quality.
    We examined the effect of treatments on the sulphur-, nitrogen content and the N/S ratio of winter wheat in the whole upperground plant and in the grain and straw at harvest in a arable land sulphur fertilization experiment on brown forest soil (Agricultural Company of Felsőzsolca).
    We analysed the samples from spring to harvesting, in the critical phenophases. In this study we discuss only the values from the stooling and stalking and the results of analysis of grain and straw in the harvest.
    We experienced that the concentration of sulphur in the whole upperground parts of winter wheat showed increase to the end of vegetation independently of fertilization. The N/S ratio was between 8% and 12% in the beginning of the growth period in the whole upperground plant, while the ratio in the grain at harvest was between 13 and 14%. When we examined the whole upperground plant, stalk and leaf at stalking, we got the highest sulphur content in the leaf. Mostly the middle level sulphur fertilization dose (4 l/ha) increased the sulphur accumulation in the green plant. At total maturing, the greatest part of accumulated sulphur is in the grain, but then the effect of fertilization is less glaring.

  • Testing a biological active plant extract’s antifungal effect against soil fungi
    247-252
    Views:
    241

    In Hungary today is about 5 million hectares of agricultural land contaminated with ragweed. The ragweed problem a year is about 60 billion HUF to be paid, of which 30 billion are used to reduce the agricultural damage. Experiments with ragweed pollen has mainly been carried out in connection with terms of allergy. The other biochemical experiments and studies with this plant, have so far been the scientific horizons of public life, boosted the edge. We wanted to demonstrate that the ragweed, which is a weed, containsbiological active (for example: antifungal) compounds. For our experiments in the previous cycle of flowering, plants were collected manually, with its roots and with each plant part. The extraction of the substance from dry plant – meal was carried out using appropriate solvents. The biological activity of ragweed-extracts were tested against fungi isolated from soils and meadow with different mode of cultivation. Our results suggest that ragweed contains biologically active substances, which inhibit the growth of fungi, depending on the concentration of active ingredients of the plant.

  • Assessment and comparison of selenium-enriched maize with sodium selenite and sodium selenate
    11-15
    Views:
    550

    Selenium is an element of environmental interest owing to the narrow range between its nutritionally required and toxic concentrations in many organisms. Its mobility and bioavailability differ greatly depending on individual Se species. In this regard, in present study, the uptake and distribution of Se, the changes in Se content, and the effects of different concentration of Se in two forms of sodium selenite and sodium selenate on maize plants were measured in nutrient solution experiments to clarify their response to the two forms of Se. The results revealed that the Se content in shoots and roots of maize plants significantly increased as the Se level increased. Two Se forms behaved differently and the effects of toxic damage in samples which had been treated with selenite were much more than in the selenate treatments.

  • The comparison of sperm motility and density in four different goldfish (Carassius auratus) types
    135-140
    Views:
    353

    Different goldfish types play an important role both in ornamental fish farming and science. Considering its historical background, the goldfish is a suitable model animal for the study of artificial selection as well as for developmental biological studies. Sperm motility and cell density is an important parameter in determining sperm quality. The aim of our study was to examine the effects of different goldfish types on the sperm quality. Several sperm motility parameters (progressive motility (pMOT, %), straight line velocity (VSL, μm s-1), curvilinear velocity (VCL, μm s-1), linearity (LIN, %), amplitude lateral Head Displacement (ALH, μm), Beat Cross Frequency (BCF, Hz)) of four different goldfish types (Common goldfish-“wild type” N=5, Shubunkin N=4, Black Moor N=4, Oranda N=5) was compared during 60 hours (at 12-hour intervals) at refrigerated storage (4 °C). The variability of sperm density was also investigated in all types. A similar cell concentration was determined in the four goldfish variants (Common goldfish 2.01*1010 ± 3.46*109; Shubunkin 1.71*1010 ± 3.25*109; Black Moor 1.66*1010 ± 3.02*109; Oranda 1.56*1010 ± 5.83*109). Statistically significant difference between the 4 goldfish types in the motility parameters and cell density was not noted. However, a decreasing tendency in Black Moor sperm motility parameters (pMOT, VCL and VSL) was observable, as well as a reduced spermatozoa density in Oranda was also recorded. Our results can contribute to the improvement of the common hatchery propagation of goldfish. Future studies can add more evidence of the possible effects of artificial selection on the reproduction in different goldfish types.

     

  • A tárolási feltételek hatása a kukorica Fusarium fertőzöttségére és toxin szennyezettségére
    28-32
    Views:
    1262

    Corn samples harvested in 1997, 1998 and 1999 from different soil types were stored at different conditions (temperature, moisture content, state of kernels) for six months. The Fusarium contamination was examined by plate dilution method and the amount of mycotoxins (F-2, T-2, HT-2, DON, DAS) were determined applying HPLC method immediately after harvesting and in the third and sixth month of storage. The aim of our study was to find correlation between the ecological factors, storage conditions and the examined parameters mentioned above, as well as to prove them statistically. According to the examinations carried out after harvesting we could state that the soil type had no effect on the parameters. Analysing the effect of the years we found considerable differences. The Fusarium infection of corn samples in 1998, while the toxin contamination in 1999 was the highest. The results of storage experiment show that year (number of microscopic fungi, F-2, T-2, HT-2, DAS and total toxin content) and moisture content of kernels (F-2, T-2, and total toxin content) have a significant effect on the examined parameters. We could prove the effect of temperature on the T-2 content (samples with natural moisture content) and DON content (samples with 14% moisture content). Higher values were found at higher temperature storage. The ratio of damaged kernels influenced the DON concentration in the non- wetted samples and the number of microscopic fungi in the wetted samples.

  • Effect of ethephon levels and amino acids on the growth characteristics of oat crop
    57-61
    Views:
    261

    A field experiment was conducted during the 2021-2022 season in an agricultural field in Basra Governorate. The aim was to study the effect of spraying ethephon and amino acids on the growth characteristics of oats (Avena sativa L.) Shifa cultivar. The experiment involved three levels of ethephon (0, 0.240, and 0.600 kg ha-1), represented by the symbols E0, E1, and E2 respectively, and two spray levels of amino acids (0 and 5 ml L-1), represented by the symbols A0 and A1. A factorial experiment was applied using the R.C.B.D (randomized complete block design) in a split plots arrangement with three replications. The results revealed significant differences in the levels of ethephon for most of the studied characteristics. The level of 0.240 kg ha-1 (E1) was superior in terms of chlorophyll content, crop growth rate, and the number of tillers, with increases of 39.07%, 39.26%, and 16.36%, respectively, compared to the control treatment (E0). Regarding the amino acids treatments, spraying at a concentration of 5 ml L-1 (A1) demonstrated significant superiority, resulting in the highest plant height, flag leaf area, chlorophyll content, and crop growth rate. This study concludes that amino acids play a crucial role in plant growth, and the combination of 5 ml L-1 amino acids with 0.240 kg ha-1 of ethephon significantly increased chlorophyll content and crop growth rate.

  • Application of the Collision Cell (CCT) in the ICP-MS Analytical System
    120-125
    Views:
    715

    Our laboratory has seen a sharp rise in the number of requests for the analysis of smaller and smaller concentrations of elements from foods, plants, soil, organic fertiliser, irrigation and ground water, sewage, sewage sludge, raw material of food, as well as human and animal origin samples. From the above elements the inductively coupled plasma mass spectrometer is capable of analysing the smallest concentration of elements. Our method of analysis is to use our ICP-MS instrument, together with a ICP-MS with Collision Cell Technology (CCT). The CCT method has better detection limit, with 1-3 magnitudes, compared to the normal ICP-MS analytical method. The CCT has better detection limits mainly for the following elements in the periodical system: analysis of arsenic, selenium, germanium, vanadium and chromium. Additionally a collision cell can be applied for the analysis of silicium, sulphur, zink, copper, iron, calcium, magnesium and potassium in smaller concentrations.

  • Comparison of the sample preparation methods worked out for the examination of the element content of wine
    77-82
    Views:
    428

    The examination of the potentially toxic elements content of the wines is not easy task, because the most elements are in little concentration (mg kg-1 or μg kg-1) in the wine and the wines contain great amount of organic matrix. The efficient sample preparation is essential for the accurate determination of element content. The eim of our research was to determine which sample preparation method will be the most efficient in examination of wines with ICP technology. The examined wine sample was a 2008 Chardonnay from the Eger wine region. We did the sample preparation and analysis examination in University of Debrecen, Centre for Agricultural and Applied Economic Sciences, Institute of Food Science, Quality Assurance and Microbiology.
    We did the analysis examinations with ICP- MS (inductively coupled plasma mass spectroscopy). We always did the sample preparations and the examinations in three times rehearsal. The applied sample preparation methods: dilution with distilled water, open digestion and microwave digestion. 
    We were able to measure B, Al, Mn, Fe and Zn with only dilution and open sample preparation. In the smaller quantity present Sr and Ba were measurable in the wine in the case of all three methods well. We were able to measure the Co with dilution and open digestion method,  while Cr, Ni, and Te with only dilution method. In the case of arsenic we were not able to measure reliable result with dilution and open digestion method because of organic matrix and other components
    (alcohols, monosaccharides, polysaccharides, polyalcohols and inorganic salts). On the whole we are able to say that in the case of certain elements (B, Mn, Fe, Zn, Sr, Ba) the open digestion and dilution sample preparation is applicable well, however, in the case of certain elements (As, Al, V, Cr, Se, Mo, Cd, Hg, Pb) we have to develop the methods. It may be development of one of the way, if we develop sample preparation methods to examined element specifically and not
    to wine generally.

  • The recent state of cryopreservation techniques for ex-situ gene conservation and breeding purposes in small ruminants: A review
    81-87
    Views:
    540

    The viewpoint of the recent cryopreservation techniques (CT) suggests the use of a reduced volume of cryopreservation solution, high concentration of cryoprotectants and ultra-rapid cooling and warming rates help to reduce cryo-injury and maximize the viability of the preserved animal genetic resources (AnGR). The CT had now become widely accepted as one of the best methods of choice for the ex-situ conservation of AnGR due to its high success rate recorded and no-invasive nature as compared to the conventional slow rate freezing (CSRF). Rapid advances and wide acceptability of the use of assisted reproductive technologies (ART’s) particularly artificial insemination (AI) in animal breeding had resulted in a greater loss of a large number of good quality genes in virtually almost all the native breeds of animals across the globe. Small ruminant (SR) animals are not an exception in such present predicaments situation of erosion and dilution of the valuable AnGR among the native breeds. As a result of this, 148 and 16 breeds of sheep and goats respectively have already become extinct in Europe and the Caucasus. In view of the aforementioned situation, the present review aimed at exploring some of the current states of development, roles played and potentials of CT in the conservation of SR genes and genome for the immediate and future breeding purposes for sustainable development. It basically covers; animal genetic resource, the need to conserve AnGR, tools for ex situ in vitro conservation of AnGR and recent developments in breeding and cryopreservation of SR AnGR.

    Cryopreservation is playing a pivotal role in ex-situ gene conservation of AnGR. Decline in genetic diversity among SR breed population was high in Europe and the Caucasus. There is therefore, need for improvent on current stringent measures on conservation of AnGR in this region of the world.

  • Pre-treatment of wastes containing keratin in industrial conditions
    59-64
    Views:
    552

    The aim of the research was a development of a featherdegradation technology in industrial circumstances. During the experiments we determined the parameters of the critical environmental and technological limitation factors. Because of the high keratin-content the degradation (and composting) of the feather is difficult. With the developed technology huge mass of feather can be used fast and easily in biogas production and in the adjustment of the optimal element ratio of biomass. The industrial experiments were implemented in a 6m3 heatable double-walled
    tank with stirring-shovels and aeration-system. The degradation process was followed with extinction measurements.
    According to the experiments that were done the best results were given at the case which was heat-treated at 70°C, was injected with 1% bacteria concentration, and where 1:3 feather:water ratio was set if we consider the effectiveness of degradation and the hydraulical retention time.