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Effect of sowing technology on the yield and harvest grain moisture content of maize (Zea mays L.) hybrids with different genotypes
17-22Views:162From the aspect of the efficiency of maize production harvest grain moisture content shall be considered beside the amount of harvested grain yield. Hybrids with different genotypes and vegetation period length lose their moisture content different that is affected by row spacing and plant density – among agrotechnical production factors – depending on the given crop year. In the present research work three crop years with different weather conditions were studied (2013, 2014, and 2015). The small-plot field experiment was set up at the Látókép Field Research Centre of the University of Debrecen, Centre for Agricultural Sciences with four replications on a chernozem soil type. The effect of three factors was analysed in the experiment on yield amount and its moisture content. Factors were row spacing (45 and 76 cm), plant density (50, 70 and 90 thousand plants ha-1), while hybrids were of very early (Sarolta: FAO 290), early (DKC 4014: FAO 320, P 9175: FAO 330, P 9494: FAO 390) and medium (SY Afinity: FAO 470) ripening.
In the crop year of 2013 the highest yield was produced – regarding the average of the hybrids – by the application of a row spacing of 45 cm (4.5%, 673 kg ha-1), however there was no significant difference between the yield of the populations of different row spacings. Significant difference (14.9%, 1751 kg ha-1; 6.3%, 583 kg ha-1) could be found in case of yield between different row spacing applications in 2014 and 2015. The effect of insufficiently distributed low amount of precipitation and lasting heat days in 2015 could be revealed in yield amounts and harvest grain yield moisture content results that were lower than in the previous years. In 2015 grain yield moisture content varied between 10.3 and 13.9% in case of a row spacing of 45 cm, while by 76 cm between 11.0 and 13.9%.
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Effect of Ferilizer on the Yield of Maize (Zea mays L.)
40-46Views:144The effect of fertilization on the yield of maize was examined on chernoem soil with lime deposits at the experimental station at Látókép of the Center for Agricultural Sciences, University of Debrecen. The yields of maize were evaluated using quadratic regression function, in three years – between 2000 and 2002 – in non-irrigated and irrigated treatments. After calculating the regression equations, by derivation of the functions, we have determined the amount of fertilizers needed for maximum yield.
In the non-irrigated treatments, maximum yield and the active substance amount of fertilizer was as it follows: in 2000, yield of 9,133 t/ha with the application of 384 kg/ha mixed active substance, while in 2002 a yield of 6,289 t/ha with the application 236 kg/ha NPK active substance was achieved. In 2001, due to the favourable precipitation, a yield of 9,864 t/ha was achieved with the application of 245 kg/ha fertilizer. In the case of maximum yield, compared to the unfertilized control, the yield increase was 2,5-5 t/ha. The average increase for 1 kg of NPK fertilizer was 13-19 kg.
We also determined the necessary fertilizer dosage for maximum yield in irrigated treatments. In 2000, 10,003 t/ha with a dosage of 423 kg/ha, in 2001, 11,542 t/ha with a dosage of 277 kg/ha and in 2002, 8,596 t/ha of maximum yield could be achieved with a fertilizer treatment of 277 kg/ha in the examined three years. The yield increase, in irrigated treatments, varied between 3,9-5,9 t/ha so it was greater than in the case of non-irrigated experimetal plots. The yield increase for 1 kg fertilizer varied between 12-21 kg. -
Effect of NPK fertilization on the yield and yield stability of different maize genotypes
101-104Views:151The yielding capacity and quality parameters of 11 maize hybrids were studied in 2011 on calcareous chernozem soil in a 25-year long-term fertilization experiment in the control (without fertilization), in the base treatment of N 40 kg ha-1, P2O5 25 kg ha-1, K2O 30 kg ha-1 and in five treatments which were the multiplied doses of the base treatment. The N fertilizer was applied in the autumn and in the spring, while P and K fertilizers were applied in the autumn.The sowing time was 17–18 April, the time of harvest was 8 October. The 30-year average of precipitation (April–Sept) was 345.1 mm, the amount of precipitation did not differ greatly from that, however, its distribution was very unfavourable.
It was found that the largest yield increment (as compared to the control) was in the treatment N 40 kg ha-1, P2O5 25 kg ha-1, K2O 30 kg ha-1 in the long-term experiment. The largest yields were obtained for the hybrids P9494, PR37N01 and PR35F38 (13.64–13.71 t ha-1). Due to the dry period at the end of the summer – beginning of autumn, the grain moisture content at harvest was favourably low, 12–18% depending on the treatment and the growing season.The N fertilization significantly increased the protein content of the kernel, but the starch content of the kernel decreased (significantly in several cases) with increasing fertilizer doses and yields as compared with the control.
The highest protein content was measured in hybrids GK Boglár and Szegedi 386. The oil content was above 4% for GK Boglár, but the two hybrids were not among the best yielding hybrids in spite of their good inner content. The starch content was around 75 % without fertilization, it decreased with fertilization.
For the tested hybrids, the fertilizer dose N 120 kg ha-1, P2O5 75 kg ha-1, K2O 90 kg ha-1 can be recommended with respect to efficacy and environmental considerations. -
Growth regulators influence on stability of shoots and ascorbic acid content at cadmium and nickel joint action
9-12Views:135It is shown that for a maize the most effective protector of cadmium and nickel influence was zeastimulin, for a pea – agrostimulin.A protective function of emistim C for both cultures was insignificant. It is set that zeastimulin is influential in the increased metals absorption of a root system, however substantially (on 15% for a nickel and twice for a cadmium) reduces their translocation to above-ground part of plants. Previous treatment of pea seed by agristimulin also intensified the accumulation of nickel roots on 60% and did not influence on cadmium absorption and toxicants translocation to tissues of assimilatory organs. The protector role of growth regulators to cadmium and nickel joint influence shows up in the increase of ascorbic acid maintenance in the roots cells of both species to 34%, where as in leaves – at a maize resulted in the lowering of vitamin С amount (on 28%), but at a pea – increase on 20%.
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Correlation analysis of relative chlorophyll content and yield of maize hybrids of different genotypes
211-214Views:154In 2021, correlation between relative chlorophyll content and yield in three maize hybrids of different genotypes was examined. The data were collected at the Látókép Experimental Station of the University of Debrecen located on the Hajdúság loess ridge in Hungary. The soil of the small plot field strip plot trial, which was set up in 2011, was calcareous chernozem. Apart from the control treatment (without fertilisation), N fertiliser is applied in the form of base and top dressing. The base fertiliser containing 60 and 120 kg ha-1 N of nutrient applied in spring was followed by top dressing containing +30–30 kg ha-1 N in V6 and V12 phenophases. SPAD values measured at different phenological stages of the growing season increased by an average of about 28% up to 10 leaf stage for all three hybrids. In the pre-silking period (Vn), the relative chlorophyll content decreased by 8% on average. After an average increase of 14% in the tasselling and silking period, SPAD decreased by an average of about 29% at full maturity (R6).
For the different fertiliser treatments, higher N doses resulted in higher yields. In the basal fertiliser treatment, the A 60 N dose resulted in an average 34% increase in yield, and the A 120 N dose resulted in an average 94% increase in yield compared to the control. The 60 kg ha-1 N basal fertiliser (A60) increased in the V6 phenophase with an additional 30 kg ha-1 N resulted in an average yield increase of 26%. When 120 kg ha-1 N of basal fertiliser (A120) was increased by an additional 30 kg ha-1 N in the V6 phenophase, only the Merida hybrid showed a significant yield increase (7%). No further yield increase was observed when V690 and V6150 treatments were increased by an additional 30 kg ha-1 N in the V12 phenophase. The yield of the Armagnac hybrid decreased by almost 20%, the yield of Fornad by 3% and the yield of Merida by 1%.
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Effects of production factors on the yield and yield component of winter wheat
26-31Views:90The effect of major production factors (forecrop, fertilisation, irrigation, soil cultivation and soil preparation) on the yield components and yield of winter wheat were studied in a long-term experiment set up at the Látókép Experimental Nursery of the Agricultural Sciences Centre of the University of Debrecen. The results of regression analysis led to the following conclusions:
• In our experiments in 2000, after using maize as a forecrop –based on the results of analysis of regression – fertilisation determined the yield.
• After using pea as a forecrop, a N50 P35 K40 kg/ha fertiliser rate led to an economical increase in the yield of winter wheat.
• None of the determinative yield components varied significantly for winter wheat produced after using pea as a forecrop.
• There is a closed, significant correlation between plant height, spike length, plant and spike mass, the number of spikelets and grains per spike after using maize as forecrop. The thousand grain mass is different from the other yield components, because it is not part of the relation system of
those yield components.
• The increased yield of winter wheat after maize has been used as a forecrop is due to the positive change in grain number per spike yield component. -
Comparison of Added Value between Bioethanol Production and the Most Important Animal Production Branches Based on Concentrated Fodder, as Potential Competitors
111-115Views:90There are an enormous amount (2-3 million t/yr) of corn surplus is available year by year in Hungary. Inland utilization is an unsolved problem, whereas export facilities of raw (unprocessed) material could not be regarded as optimal way because of logistical barriers and the very low producer’s price. There are two basic opportunities for the export of the surplus of maize with reduced transportational costs and higher value:
animal production and process of bio-ethanol. In Hungarian conditions both of them demand the same raw material so they should compete with each other for maize. Both need financial aid at least for the investment in order to reach profit. Decision makers are influenced by several factors in allocating of national supports between the differential branches, one of them could be the added value developing in the given vertical change. I will introduce and analyze the expectable added values of the abovementioned competitive activities. -
Studying the effects of traits in the genotype of three maize hybrids in Hungary
97-101Views:370In this study, we selected three hybrids (Armagnac, Loupiac, and Sushi) for evaluation of the effect of traits in genotype in Debrecen. In 2017, the total rainfall from May to October was 314 mm in Debrecen, which was 236 mm in the winter period before sowing. The obtained results showed that there was a positive correlation between the weight of the cob maize and the rate of seed/cob, number of rows with number of seeds in column and outer diameter ear with weight of cob and number of rows in grain per ear and the rate of seed / cob; Also, there was a negative correlation between grain weight in ear with seed/cob rate, outer diameter ear and the rate of seed/cob and outer diameter ear with the number of leaves. There was a positive correlation between stem diameter, Seed/cob rate and the number of nodes by GGE biplot. In addition, there are traits of weight of all seeds and outer ear diameter that had the highest effect on average yield. Moreover, the number of seeds per row showed the least effect on the average yield of hybrids.
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Role of Sowing Time in Maize Production (Review)
36-39Views:93Many authors, both in Hungary and abroad, have reported on experiments carried out to determine the role of sowing time in maize, but the results are often contradictory. This is hardly surprising, since the maize plant exhibits enormous genetic variability and the hybrids created through selection and inbreeding may have very specific requirements as to sowing date. The year effect, too, often complicates the efforts of scientists to provide clear guidance to farmers on the optimal sowing date for each hybrid.
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Establishing biotic stress tolerance of maize (Zea mays L.) by measuring hydroxamic acid contents
107-112Views:206Cyclic hydroxamic acids are the most considerable secondary metabolites in grasses and their main task is to protect these species from pathogens and pests. The cyclic hydroxamic acid content and common smut susceptibility were examined in our experiments. 27 maize hybrids were used for experimental plants in a climate room, where the plants were grown on a nutrient solution. An infiltration method was used for the inoculation of the plants. The total quantity of cyclic hydroxamic acids was determined and the ratio of infected plants and the ratio of inhibition was determined, too. Based on our results, on the basis of all hybrids’ data, the total hydroxamic acid content of the infected plants was higher than in the control. On the level of individual hybrids, only 9 of them had higher cyclic hydroxamic acid content in the case of infection. Increase in cyclic hydroxamic content induced by the fungus in this case is a tool for the fungus to suppress other pathogens and pests. Amongst the hybrids’ cyclic hydroxamic acid contents, significant differences were detected in the control and in the infected treatment, too. The so-called “sweetcorn” hybrids showed high level of cyclic hydroxamic acid content. According to the differences amongst hybrids, homogenous groups were created which groups differed in the case of control and infected treatment, because of the difference in increase of cyclic hydroxamic acid content. The examined hybrids showed different levels of infection and different rate of growth inhibition for the effect of inoculation. According to the infection caused damage hybrids were ranked. Infection caused notable damage for hybrids Prelude, Desszert 73, DKC5276 and DK440.
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Violation prooxidative-antioxidant stability at maize shoots at different level of accumulation of cadmium and nickel
89-94Views:75Joint influence of cadmium and nickel was investigated on the feature of their accumulation by the vegetative organs of 10-days' old maize shoots. It was established that most intensively noted metals are taken in by the roots of shoots in the first 7 hours stressing influencing, while in leaves they appear only after a 7-hour long exposition. It was stated that the absorption process of the noted metals by a root system is carried by two-phase character. The indexes of inner-tissue contamination are calculated. Activating by the cadmium and nickel ions of lipid peroxidation as marker of the stressing influencing, and also was shown the proper increase of intensity of functioning of ascorbate peroxidase as the antioxidant enzyme protection of cell.
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The effect of water supply and crop year on the yield potential of sweet maize (Zea mays L. convar. saccharata Koern.) hybrids with different genotypes
203-210Views:201The successfulness of crop production is significantly affected by not only the the average yields that provide cost effectiveness, but also the success of striving for yield safety, therefore, varieties and hybrids tolerant to environmental stress factors are worth being included into the sowing structure. Our aim was to further the decision making of producers in prepaering the right sowing structure by the evaluation of sweet maize hybrids’ tolerance to excess rainfall.
We performed our examinations in an extremely wet year (2010) on chernozem soil on three sweet maize hybrids (GSS 8529, GSS 1477, Overland) in 12 replications. Comparing the yields of 2010 with those that can be expected under optimal rainfall conditions, we showed that the examined hybrids react to the amount of rainfall higher than their needs with yield depression. The excess rainfall tolerance of the examined hybrids is different in the case of each hybrid. -
Ecotoxicological impact of DON toxin on maize (Zea mays L.) germination
35-40Views:211Fusarium graminearum is one of the most significant arable pathogen in Hungary, and various types of trichothecene mycotoxins (mostly DON, deoxynivalenol) are detected most commonly in cereals (Biró et al., 2011). Fusarium infection and mycotoxin production could not be eliminated, and infected maize by Fusarium sp. cannot be exploited as food, seed, or animal feed. However it can be raw material of biogas production. In this research we would like to investigate the content and effect of the toxin in the end product of biogas production on plant germination. The Fusarium sp. can cause mildew and seedling mortality in seed of maize (Zea mays L.), so we examine the effect of this on germination. In preliminary examination Fusarium sp. was not detected in the bioreactor of the Institute after the retention time (30 day), however it can be assumed that during the hydrolysis of the fungus growth and mycotoxin production also increased exponentially. There were no appropriate tools to detect the toxin in the end product of biogas production so modelling of anaerobic hydrolysis was necessary. The effects of hydrolyzed product for germination were also detected.
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The impact of climatic factors on the relative chlorophyll content and yield of a maize hybrid in a long-term experiment
71-77Views:234The impact of the climatic factors of crop year on the relative chlorophyll content of maize was examined for three years. The examinations were carried out on the Látókép Experiment Site of the University of Debrecen on calcareous chernozem soil in a small-plot, non-irrigated long-term field experiment with strip plot design. In addition to a non-fertilised (control) treatment, nitrogen (N) fertiliser doses were applied as base and top dressing. The 60 and 120 kg N ha-1 base dressing doses were followed by two top dressing doses at the V6 and V12 phenophases.
Averaged over the different fertiliser treatments, SPAD readings increased in all three years as the growing season progressed. The highes SPAD value increase was observed in the average crop year (2017) at the V12 phenophase (11.8), which further increased at the R1 phenophas, by 3,7. No significant Spad value difference was observed between the average (2017) and the dry year (2018) at the V6 growth phase. However, in the wet crop year (2016), the V690 treatment provided the statistically highest relative chlorophyll content (46.8). At the V12 phenophase, the base dressing dose of 120 kg N ha-1+30 kg N ha-1 (V6150) showed to be successful in two years (2016 and 2018), while in 2017, the base dressing dose of A60 was successful. The impact of crop year on relative chlorophyll content can be clearly shown at the R1 growth stage. In all three years, the significantly highest relative chlorophyll content could be achieved at different nutrient levels: A60 in 2016, V6150 in 2017 and V690.
In a wet year (2016), higher yield could be achieved as a result of the 60 kg N ha-1 base dressing and 30 kg N ha-1 at the V6 growth stage (V690) as top dressing in comparison with 2017 and 2018, when higher fertiliser dose (120 kg N ha-1 base dressing and 30 kg N ha-1top dressing at the V6 growth stage) was needed to achieve a significant yield surplus.
Altogether, averaged over the different treatments, the highest yield (12.48 t ha-1) was observed in the wet year, when the relative chlorophyll content was also the highest (50.6).
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Changes of relative chlorophyll content at maize smut inoculated hybrids
55-58Views:285The leaf chlorophyll content analysis is important for several reasons. The natural or anthropogenic stressors directly effect on the chlorophyll content. Through the measurement of the chlorophyll content it is possible to obtain data concerning the physiological status of the plant, moreover the chlorophyll content is closely related to the nitrogen content, so it is linked to photosynthesis and the photosynthetic activity which determine biomass production.
One of the most common symptoms of plant diseases is the larger and smaller interveinal chlorotic areas. These might be local, or expand to the whole plant. There are multiply reasons of chlorosis such as reduction of chlorophyll content, unfavorable effects on the chlorophyll content,disorders regarding function of chloroplasts or ultimately destruction of the chloroplasts. Although such a chlorotic deviancy can contribute to significant losses in photosynthesis; however the underperformance photosynthesis of the sick plants is a more complex process.
As we unambiguously experienced during our investigations on common smut that the infected maize plants most common accompanying symptom was chlorosis on the leaves, so it is especially important to examine how the infection influenced on the chlorophyll content of different hybrids. -
Computer simulation modeling of Leaf Area Index (LAI) in maize
5-8Views:159This study presents a PHP-based model capable of calculating maize leaf area index. The model calculates LAI from emergence to 75% silking. The basis of calculation is represented by the daily average temperature values. The usability of the model was tested using three years' temperature and LAI data series from the values obtained by the weather station set up at the Látókép Experiment Site of the University of Debrecen, Centre for Agricultural Sciences between 1994 and 1996. During the running of the model, it was observed that temperature affects the intensity of leaf development to a various extent.
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Susceptibility of maize hybrids to corn smut diseas
39-42Views:189Corn smut disease is one of the most common maize diseases. In the previous years the disease lost from its importance, however nowadays it is becoming more and more important and widespread. The protection against it encounters great difficulties, therefore it is important to emphasize prevention and resistance based protection. The aim of the research was to investigate under laboratory circumstances the resistance of Hungary’s most commonly cultivated maize hybrids to corn smut disease. Twelve species were investigated. We distinguished two susceptibility groups within the hybrids based on the investigated parameters. The results are intended to assist the protection against corn smut disease and to make a suggestion for the farmers so that they could apply more environmental friendly and economical production.
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Effect of cultivation factors on the yield and yield security of maize
263-265Views:150Cultivation factors have a significant effect on the yield and yield security of maize. Ensuring a suitable green crop is important. Tricultural crop rotation (pea–wheat–maize) in the average of 25 years provided a 2 t ha-1 higher yield compared to monocultural cultivation. A harmonious NPK nutriment supply determines yield and yield security, which can be especially realized by means of the application of precision cultivation technologies. Under average circumstances N 80 kg ha-1, P2O5 50 kg ha-1, K20 60 kg ha-1 active ingredient is the agro-ecological dosage of artificial fertilizer.
Plant density is a factor that determines yield. Optimal plant density – beside the genetic characteristics of the hybrid – is mostly influenced by the level of water and nutriment supply. -
Examination of the impact of sowing technology models on the ear, constiuent and yield parameters of the yield formation elements of maize hybrids of different genotypes
17-23Views:141Production year 2012 has been characterised by climatic extremities. The weather of this year can be considered very contradictory in terms of maize production. The droughty conditions of the winter and spring months had a negative effect on both germination and starting vigour. The favourable weather of May-July created ideal conditions for intensive growth and generative processes; however the lack of precipitation in August and September had a damaging effect on the development of yield composing elements and grain saturation processes as well. Under such circumstances, the sowing date models caused significant differences in the yield and quality of the hybrids belonging to different growth periods. The growing period of the maize hybrids has been shortened as a result of the unfavourable climatic conditions.
Based on the trial results, it is verifiable that short growing period hybrids can be securely sown in draughty years even with a later sowing date, however using a later sowing date in the case of longer growth period hybrids may result even in a yield loss of 2–3 t ha-1. In the case of early and average sowing dates, with given yearly conditions the hybrids of the observed FAO 370-390 hybrid group provided the best result (12.40 t ha-1, 10.99 t ha-1), while in the case of the third, late sowing date the yield dominance of the FAO 290-350 hybrid group is the most significant (10.08 t ha-1).
The analysis of the yield composing elements found that the P9578 hybrid has the highest shelling ratio, while its cob is the shortest. The P9494 hybrid has a high yield and the highest thousand grain weight, while the DKC 4983 has the longest cob and its thousand grain weight is above 300 g.
The results confirm the fact that DKC 4590 has the highest yield potential and starch content, while in terms of oil and protein content the Szegedi 386 and NK Octet hybrids are the most important.
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Evaluation of reduced tillage technologies in corn production based on soil and crop analyses
47-54Views:177Despite new cultivation methods, the proportion of conventionally cultivated land is still very high in Hungary.
Although these technologies demand more time, labour and fuel, they are still attractive to users because they require less professional skill and simple machinery. In Hungary, conventional tillage methods usually lead to soil deterioration, soil compaction and a decrease in organic content. These side effects have caused gradually strengthening economic and environmental problems.
The technologies for those plants which are dominant on Hungarian arable lands use (winter wheat, maize, sunflower and barley) need to be improved both in the interest of environmental protection and the reduction of cultivation costs.
The Department of Land Use at Debrecen University is cooperating with KITE Sc. to carry out soil tillage experiments at two pilot locations to prove tillage technologies already used in the USA.
The aim of our examination is to adapt new technological developments and machinery, and to improve them on Hungarian soil for local environmental conditions. With these improved machines, the field growing of plants could be executed by less manipulation and better suited to economic and environmental needs. The most significant task is to investigate and improve the conventional cultivation replacing, new soil-protecting tillage technologies, and to apply no-till and mulch tillage systems.
On the basis of the experiments’ survey data, we established that the looseness and moisture content of the soil using reduced tillage is more favourable than after using conventional technologies. The results of no-till and shallow spring tillage are behind those of winter plough or disk ripper cultivation in corn yield and production elements.
To preserve moisture content in the soil, the ground clearing and sowing while simultaneously performing no-till method presents the most favourable results. The surplus moisture gained using no-till technology is equal to 40 mm precipitation.
Regarding the yield of winter wheat we established that the tillage methods do not affect plant yield. Both disk ripper and conventional disc cultivation showed nearly the same harvest results (5.55 or 5.5 t/ha), where the difference is statistically hardly verifiable from the no-till method. From the individual production of corn and the number of plants planted in unit area, calculated results prove that no significant difference can be detected between the production of winter plough and disk ripper technology. Although the yield achieved with the no-till method is less than with the previously mentioned technologies, the difference is only 9-10%. We received the lowest production at shallow spring tillage.
Evaluations have shown a 1.1 t/ha (13%) difference in the yield of maize, between winter tillage and the disk ripper method, in this case the traditional method resulted in higher yield. In winter tillage, the yield of maize was 1.9-2.1 t/ha (23-25%) higher than in the case of direct sowing and cultivator treatments. No significant difference could be noted between the yields of direct sowing and cultivator treatments.
Our research so far has proved the industrial application of reduced tillage methods in crop cultivation technologies. -
Comparison of Variability among Irradiated and Control Inbred Maize Lines via Morphological Descriptions and Some Quantitative Features
70-73Views:78Knowledge of genetic diversity in breeding material is fundamental for hybrid selection programs and for germplasm preservation as well. Research has been done with nine irradiated (fast neutron) and four non-treated inbred lines. The aims of this study were (1) to investigate the degree of genetic variability detected with morphological description (based on CPVO TP/2/2) in these materials, (2) to compare the genetic changes among irradiated and non-irradiated maize inbred lines (based on some quantitative features). The irradiation did not change any of the characteristics clearly in positive or negative way, which can be related to the fact that the effect of induced mutation on genetic structure cannot be controlled. From the irradiated lines we have managed to select plants with earlier ripening times and better phenotypes. We could distinguish 3 main groups by the morphological features; these results match our expectations based on pedigree data. Markers distinguishable on the phenotypic level (e.g. antocyanin colouration, length of tassels) were significant in all lines.
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Comparative analysis of soil analysing datas on different sempling-plots
85-90Views:86Hibrid maize is cultivated on larger plots, therefore the sown areas of hibrid maize are heterogeneous from a pedology aspect. Heterogenity causes problems during tasseling, chemical plant protection and harvest. The heterogenity of sown areas can be compensated by fertilization which is based on soil analysis. We carried out research into change of the soil on four soil types from 1987 to 2005.
There were no significant changes in pH, hydroiodic acidity, CaCO3-content, humus-content on meadow chernozem soil. We detected equalization of salin content in the examined soil layers. There were no significant changes in the measured values on chernozem meadow soil and solonetz meadow soil in 2005. We discoverd equalization of saline content on chernozem meadow soil, but the changes were not as obvious as the changes on meadow chernozem soil. We found salinization in the 30-60 cm soil layer on type meadow soil that may be due to water movement. -
Comparison of RAPD and AFLP Analysis in Some Maize (Zea mays L.) Lines and Hybrids
3-7Views:114The use of molecular markers to enhance plant breeding efforts is being widely studied. DNA-based fingerprinting technologies (RAPD and AFLP) have proven useful in genetic similarity studies. We estimated different maize (Zea mays L.) inbred lines and hybrids originated from mutant ones based on their genetic differences.
We carried out RAPD analysis with different primers and the 707 (CCCAACACCC) and 792 (CAACCCACAC) primers with 50% similarities provided quite good DNA fragments. By applying the DNA based-AFLP technique, we had very dense DNA fingerprinting. We differentiated 15-32 polymorphic bands, the highest number of bands were found in P-T/H-CA (32). AFLP seems to be the more efficient method of comparing genetic similarities/differences among different genotypes. -
Germination and sugar content alteration in maize grain caused by Fusarium contamination
42-44Views:74Healthy and Fusarium affected ears were collected in Fészerlak, Somogy County at the end of vegetation cycle of maize (25-30 August). Each pattern contains 25-25 ears. We compared the samples on the basis of visual image of Fusarium affection. The ears were shelled and two 0,5 kg samples were formed: healthy and Fusarium contaminated. After surface sterilisation the uniform sized seeds were soaked in sterile distilled water for 24 hours and there were germinated for 7 days. The α-amylase activity was measured with Phadebas- α-amylase test. Seeds were extracted one by one three times under reflux using 10 cm3 boiling water for 15 minutes. During our investigation germinating activity was detected to measure glucose, fructose, sucrose content and α-amylase activity. In the first seven days of germination the highest values were detected in control seeds followed by the affected seeds. Our results clearly show that stress conditions applied altered not only the saccharide content but decreased their germinating activity as well in the case of maize grain.
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Development of a New Maize (Zea mays L.) Breeding Program
25-30Views:113Genetic manipulation may not replace any conventional method in crop breeding programs, but it can be an important adjunct to them. Plant regeneration via tissue culture is becoming increasingly more common in monocots such as corn (Zea mays L.). In vitro culturability and regeneration ability of corn decreased as homozigosity increased, which suggested that these two attributes were controlled primarily by dominant gene action. Pollen (gametophytic) selection for resistance to aflatoxin in corn can greatly facilitate recurrent selection and screening of germplasm for resistance at a much less cost and shorter time than field testing. Integration of in vivo and in vitro techniques in maize breeding program has been developed to obtain desirable agronomic attributes, speed up the breeding process and enhance the genes responsible for them. The efficiency of anther and tissue cultures in most cereals such as maize and wheat have reached the stage where it can be used in breeding programs to some extent and many new cultivars produced by genetic manipulation have now reached the market.