This paper aims to present the measurement tool designed to examine gender differences among teacher education students and the results obtained during the research. The theoretical section of the paper describes teachers’ and parents’ influence on children’s gender role attitudes, gender socialization and career orientation. To prepare the questionnaire, we analyzed the Hungarian and international literature and built on the results of our previous qualitative research. We grouped our questions into three dimensions: (1) gender socialization in the family, (2) parental involvement, (3) gender socialization at school and career orientation. According to our results, gender-neutral toys were more often chosen by male students’ parents, and mothers were more involved in their children’s school activities than fathers, setting higher expectations as well. Teachers and parents, especially mothers, played an important role in students’ career choice. In addition, students perceived that teachers handled them differently depending on their gender in terms of the evaluation of their academic progress and behavior as well as the frequency of compliments and punishment.