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The Effect of Soil Coverings on Cellulose Decomposition Activity of Sandy Soil
15-20Views:182The purpose of our experiments is to discover the effect of different soil cover matter (agroszövet and black polyethylene) on actual (under field circumstances) and potential (under laboratory circumstances) cellulose decomposition activity. In our field research, the Unger-test was used, and in laboratory research, the Petkov-Markova method was applied. In the first year of the experiment (2000) actual cellulose decomposition activity was significantly higher in covered than in the uncovered soil both in spring and autumn. The difference between the two treatments was significant only in spring. In the spring of 2001 black polyethylene showed significantly the lowest, activity, while in autumn the agroszövet (a porous black polyethylene) showed significantly the highest activity. In the autumn of 2001 the soil covered by black polyethylene gave non-significant,and the soil covered by agroszövet gave a significant higher activity value than the control. Averaging the two experimental years (2000-2001), the actual cellulose decomposition activity was significantly higher in covered soils both in spring (with 30-39%) and in autumn (with 34-69%). Moreover, in autumn a significantly higher value was detected under agroszövet than in any other treatment. The difference between the effect of treatments was not significant. In 2000, the potential cellulose decomposition activity was the highest in soil covered by agroszövet in spring, but in autumn higher activity value was detected in every covered soil than in the control. In the spring of 2001, every covered soil showed a lower, but in autumn a higher, potential cellulose decomposition rate than the control. The activity decreased significantly 27 (agroszövet) and 45 (black polyethylene) percent in spring, and increased no- significantly 8 (agroszövet) and 4 (black polyethylene) percent in autumn. During the two experimental years, we observed on average lower potential cellulose decomposition activity (15-60%) in spring and a higher one (14-43%) in autumn. Neither was significant. The dynamic of potential cellulose decomposition activity averaging 2000 and 2001 showed a slight, the actual cellulose decomposition activity an explicit non-significant upward tendency. There was a strong (r=0,189) correlation obtained between the actual and potential cellulose decomposition activity of soil, and a medium-strong (r=0,673) relationship between the soil moisture content and actual cellulose decomposition activity.
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Effect of Soil Covering on CO2 Production and Cellulose Decomposition Activity
198-201Views:124In our experiments the effect of different soil cover matters (agroszövet and black polyethylene) on the CO2 production capacity and on the soil cellulose decomposition activity was studied. We also examined the relationship between the CO2 production and the cellulose decomposition activity. The trial was carried out in an apple plantation in Újfehértó. The CO2 production were determined after Witkamp with 5 days’ incubation period, the cellulose decomposition activity was measured according to Unger. Results showed that CO2 production and cellulose decomposition activity was higher in control soil almost in every cases. In respect of CO2 production the black polyethylene gave better values than the agroszövet.
In 2000 the cellulose decomposition activity was significantly higher under covered soil than under uncovered every time. In spring of 2001 the controll but in summer and autumn agroszövet was significantly better. In summer and autumn black polyethylene prove non-significantly better than control. In average of two experimental years significantly higher cellulose decomposition activity was detected in covered soils than in control.
In the most occassion a close connection was observed between cellulose decomposition activity and CO2 production. In spite of this the correlation was not significant in controll at all. Significant relationship was only found in spring and summer of 2001 under agroszövet, in spring and autumn of 2000 and in summer of 2001 under black polyethylene. -
Comparative examination of a mineral fertiliser and a bacterial fertilizer on humic sandy soil
111-116Views:315In our pot experiment, the impact of a bacterial fertilizer, Bactofil® A10 and a mineral fertilizer Ca(NO3)2 applied in different rates was studied on some soil chemical and microbiological characteristics of a humic sandy soil (Pallag). Perennial rye-grass (Lolium perenne L.) was used as a test-plant. Samples were collected four and eight weeks after sowing in each year. The experiment was set up in 2007-2009 in the greenhouse of
the UD CASE Department of Agrochemistry and Soil Science. The available (AL-extractable) nutrient contents of soil, among the microbial parameters the total number of bacteria, the number of microscopic fungi, cellulose-decomposing and nitrifying bacteria, the sacharase and urease enzyme activity, as well as the soil respiration rate were measured.
Statistical analyses were made by means of the measurements deviation, LSD values at the P=0.05 level and correlation coefficients were calculated. Results of our experiment were summarised as follows:
− The readily available nutrient content of humic sandy soil increased as affected by the treatments, in case of the available (AL-extractable) phosphorus and potassium content the higher value was measured in high-dosage artificial fertilizer treatment.
− The treatments had also positive effect on several soil microbial parameters studied. The higher-dosage mineral fertilizer treatments had a beneficial effect on the total number of bacteria, cellulose-decomposing and nitrifying bacteria. No significant differences were obtained between the effect of treatment in case of the total-number of bacteria, the number of microscopic fungi and nitrifying bacteria.
− On the sacharase enzyme activity the artificial fertiliser treatments proved to be unambiguously stimulating, the urease activity significantly increased on the effect of the lower-dosage Ca(NO3)2 artificial fertilizer treatment.
− The soil respiration increased in all treatments in related to the amounts applied, significantly increased in the highest rate of Ca(NO3)2 fertilizer addition.
− Some medium and tight positive correlations were observed between the soil chemical and microbiological parameters studied in case of both nutrient sources.
Summarizing our results, it was established that the organic and all the mineral fertilizer treatments had beneficial effects on the major soil characteristics from the aspect of nutrient supply. In majority of the examined soil parameters (AL-extractable phosphorus- and potassium, total number of bacteria, number of cellulose-decomposing and nitrifying bacteria, activity of sacharase enzyme) the high rate of Ca(NO3)2 mineral fertilizer treatment proved to be more stimulating, but at the same time the high rate bacterium fertilizer resulted in significant increases in
the nitrate-N content, the AL-potassium content of soil, the total number of bacteria, the number of cellulose-decomposing and nitrifying bacteria and the urease enyme activity.
Our examinations showed that the mineral fertilizer treatments proved to be more stimulating on most of the soil parameters studied but according to our results, it was established that Bactofil is efficiently applicable in the maintenance of soil fertility and the combined application of
mineral fertilizer and bacterium fertilizer may be a favourable opportunity – also in aspect of the environmental protection – in maintaining soil fertility. -
The effect of bentonite on the quantity change of soil microorganisms, the CO2-production and the activity of saccharase enzyme
287-293Views:245We examined the impact of bentonite – the perspective improving material of sandy soils – and treatments of livestock manure composted with bentonite on sandy soils, within the framework of a small-plot experiment.
The adjustment of the experiment was made on the Experiment Site of the Nyíregyháza Research Centre of the University of Debrecen, Centre of Agricultural Sciences (UD CAS). We collected soil samples from parcels treated with increasing bentonite doses (5, 10, 15, 20 t/ha) on the one hand, and from the parcels treated with livestock manure composted with increasing doses of bentonite, on the other.
We performed laboratory research in the soil microbiology laboratory of the Soil Science Faculty of UD CAS DAS, during which we determined the total number of bacteria, the quantity of microscopic fungi, the number of cellulose-decomposing bacteria, the CO2-production of the soil and the activity of saccharase enzyme.
During the evaluation of the examinations, we made a statistical analysis using SPSS 9.0. We determined the average of measurements, the standard deviation of controls, the standard deviation, the significance value and we also performed a correlation analysis.
Concerning the impacts of bentonite treatment and the treatment of livestock manure composted with bentonite on the examined microbiological features of sandy soil, we can summarize the following:
• Our results prove that the microbiologic activity of the soil has increased owing to the impact of bentonite treatments regarding total number of bacteria and the quantity of microscopic fungi. ”Pure bentonite” treatments – although not significantly in every case – increased these values, but larger doses decreased them. The treatments of livestock manure composted with bentonite resulted in a larger increase regarding both parameters.
• The number of cellulose-decomposing bacteria was increased by the low doses of both series, and was decreased by the higher doses. Higher bentonite doses decreased it in a higher – significant – degree than those of treatments of livestock manure composted with bentonite, whose low dose caused salient number of bacteria.
• Regarding the carbon-dioxide formation, we have experienced an increase even in the case of low dose treatments (nevertheless, the increase did not prove to be significant), but – similarly to the quantitative changes in the number of cellulose-decomposing bacteria – the large doses of both series of treatment decreased the CO2-production of the soil.
• Bentonite also increased the activity of saccharase enzyme significantly. We learned that”pure bentonite” treatments increased the activity of the enzyme to a higher degree than composted treatments. Moreover, it can be stated that the treatments of larger doses of both bentonite and livestock manure composted with bentonite have decreased the enzyme activity – not significantly, though.
• Based on the correlation analysis, it can be stated that as an impact of the treatments, the microbiological activity of the soil has also increased with the increase of the number of soil microbes, as in both treatment series we have experienced a tight positive correlation (r=0.81-0.82) between the change of total number of bacteria and the CO2-production of soil. In both treatments, there was a medium correlation between the total number of fungi and soil respiration (r=0.63-0.63). Furthermore, it can be stated that the usage of organic manure had a positive effect on the activity of cellulose-decomposing bacteria, as in this treatment series there was a positive correlation (r=0.65) between this physiological group and CO2-production. Both treatments prosperously impacted the activity of saccharase enzyme, because there was a medium correlation (r=0.62-0.64) between the activity of the enzyme and soil respiration. -
Development opportunities of biomass-based ethanol production in relation to starch- and cellulosebased bioethanol production
71-75Views:298The biomass is such a row material that is available in large quantities and it can be utilizied by the biotechnology in the future. Nowadays the technology which can process ligno cellulose and break down into fermentable sugars is being researched. One possible field of use of biomass is the liquid fuel production such as ethanol production. Based on the literary life cycle analysis, I compared the starch-based (first generation) to cellulose-based (second generation) bioethanol production in my study considering into account various environmental factors (land use, raw material production, energy balance). After my examination I came to the conclusion that the use of bioethanol, independent of its production technology, is favorable from environmental point of view but the application of second generation bioethanol has greater environmentally benefits.
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Comparative examination of a bacterium preparation (BACTOFIL® A10) and an artificial fertilizer [CA(NO3)2] on calcareous chernozem soil
75-80Views:368In a small-pot experiment a bacterium preparation, Bactofil® A10 and an artificial fertilizer containing Ca(NO3)2 in different dosages were studied on calcareous chernozem soil, concerning the readily available nutrient content of soil (nitrate-nitrogen, AL-phosphorus, ALpotassium content of soil, some soil microbial characteristics (total number of bacteria and fungi, cellulose-decomposing and nitrifying bacteria, CO2-production of soil), and the amount of the plant biomass.
The readily available nutrient content of the calcareous chernozem soil increased due to the treatments, except for the change in the soil nitrate-nitrogen content, which did not measure up to the control due to the effect of high-dosage Bactofil.
The treatments also influenced the examined microbial characteristics of the soil positively. The artificial treatments significantly increased the total number of bacteria and the number of cellulose-decomposing and nitrifying bacteria. The low-dosage Bactofil significantly increased the number of cellulose-decomposing bacteria and both Bactofil dosage significantly increased the number of nitrifying bacteria. The measure of the soil respiration grew in all treatments, but significantly only in Ca(NO3)2 fertiliser treatments.
The quantity of the plant biomass was grew in a low-dosage Bactofil and significantly in the artificial fertiliser treatments. The highest plant biomass quantity was measured in the high-dosage artificial fertiliser treatment.
In the correlation analyses we found some medium positive correlation between the soil chemical, microbiological parameters examined, and the plant biomass in the case of both treatment-forms.
Based on our results Ca(NO3)2 artificial fertiliser treatments on calcareous chernozem soil proved to be more stimulating regarding the
examined soil characteristics and the amount of the plant biomass, but the low-dosage Bactofil also positively influenced the majority of the
soil characteristics examined in terms of nutrient supply. -
The impact of different fertilization methods on some microbiological soil characteristics
119-126Views:318In our experiment, we studied the impact of an organic fertilizer, Bactofil® A10 (half- and full dosage applied in field practice) and an artificial fertilizer of Ca(NO3)2 content in different dosages (20-40 mg kg-1) – in addition to control treatments – on two different soils (calcareous chernozem, humus sandy soil) in 2005-2006, the experiment was complemented with treatments applying 250% dosage (100 mg kg-1 N, Bactofil® A10 2.5 times the field dosage) and a compost from urban sewage (25 g kg-1 compost) was also tested on these two soil types. In the
experiment, several soil microbial parameters were studied. The experiment was set up at the Department of Agrochemistry and Soil Science using 1-kg pots.
Our laboratory experiments were performed at the soil microbiology laboratory of UD CAS Department of Agrochemistry and Soil Science, the total number of bacteria, microscopic fungi, nitrifying and aerob cellulose-decomposing bacteria were determined together with the CO2-production of soil, N content of the biomass and urease enzyme activity.
Statistical analysis of the data was done using the program SPSS 13.0, means of the measurements, deviation and significance values were calculated.
In 2005-2006, the effect of the different dosages of Bactofil® A10, and the Ca(NO3)2 fertilizer on the examined microbial parameters of calcareous chernozem and humus sandy soils can be summarized as follows:
• Concerning the total number of bacteria, both treatments were effective on calcareous chernozem soil, the higher (significant) increment in bacteria number was observed in the artificial fertilizer treatments, while in the humus sandy soil Bactofil treatments had a beneficial effect. The number of microscopic fungi also increased in both treatments, higher numbers were observed in the average of two years in the Bactofil treatments.
• The number of nitrifying bacteria was 2.5 times higher in both high-dosage treatments on calcareous chernozem soil, while on humus sandy soil a slight (not significant) increment was observed only int he high-dosage Bactofil treatment. The amount of aerob cellulose-decomposing bacteria significantly increased on calcareous chernozem soil in both the highdosage artificial fertilizer and the small-dosage Bactofil treatment, however, on humus sandy soil no significant increase was observed in either treatment.
• The CO2-production increased in both soil types, although it was not significant in either treatment. A higher (though not significant) soil respiration was observed in the Bactofil treatments in both soil types.
• The microbial biomass N values were significantly higher in the high-dosage Bactofil treatments, however, the high-dosage artificial fertilizer treatment also increased these values significantly on calcareous chernozem soil.
• On calcareous chernozem soil, urease activity was significantly increased and reduced by high-dosage artificial fertilizer treatments and Bactofil treatments, respectively. On humus sandy soil, urease activity was also reduced except for the high-dosage artificial fertilizer treatment. In 2007, the pot experiment with 250% dosages was complemented with the application of compost rich in organic matter, the results of these treatments are sumnmarized as follows:
• In the case of the total number of bacteria, all three treatments resulted in a significant increase on calcareous chernozem soil with the highest values in the Bactofil treatment. The Bactofil treatment was the most effective on the humus sandy soil, but the artificial fertilizer treatment also
resulted in a significant increment. In the case of the total number of fungi, Bactofil treatments resulted in the highest values on both soils, but the compost treatment also increased the number of fungi in calcareous chernozem significantly.
• The number of nitrifying bacteria was increased most (significantly) by the Bactofil and compost treatments on both soil types. The amount of cellulose-decomposing bacteria was significantly increased by he compost treatment on calcareous chernozem soil, while its effect was not significant on humus sandy soil. The number of these bacteria was increased significantly by the Bactofil treatment on humus sandy soil.
• On calcareous chernozem soil, all three treatments significantly increased CO2-production, while the compost treatments had the resulted in the largest increment in soil respiration on both soil types.
• The soil biomass N content was significantly increased in both soils by the compost treatment, while in the case of the humus sandy soil, the Bactofil treatment also resulted in a significant increment.
• Urease enzyme activity was significantly increased by the artificial fertilizer treatment on both soils. In calcareous chernozem soil, the Bactofil treatment resulted in a slight (not significant) reduction in enzyme activity. In humus sandy soil, the Bactoful treatment also resulted in a slight reduction, while the compost treatment increased (though not significantly) the urease activity.
Based on our results, it can be stated that all three treatments were effective with respect to the studied soil microbial parameters. For both the calcareous chernozem and the humus sandy soil, the organic fertilizer Bactofil and the compost with high organic matter content had a stronger effect on some soil microbial parameters than the artificial fertilizer. -
The effect of different herbicide on the number and activity of living microorganisms in soil
76-82Views:258Sustainable plant growth, considering the difficulties of weed elimination, cannot be effective without the application of herbicides. However, these chemicals have enormous ecological implications, including effects on the microbiological communities of soils. It is advisable to use herbicides that have minimal secondary effects on the environment and soil-living microorganisms. In contrast, herbicides with prolonged growth stimulating or inhibiting effects are not suitable, because both types have strong influences on the number and activity of bacteria, thus causing changes in the ecological equilibrium.
Preceding small plot experiments, laboratory tests were carried out to study the effect of herbicides used in maize cultures on the number of bacteria and growth of microscopic fungi.
Substances that were observed to have stronger influences were applied in small plot experiments set up in the experimental garden of the Department of Plant Protection of the University of Debrecen. We studied the effects of four herbicides (Acenit A88EC, Frontier 900 EC, Merlin SC and Wing EC) on the microbiological properties of the soil. These herbicides were used in different concentrations in maize culture, and we investigated the effects in different soil layers.
In the laboratory experiments, we determined the total number of bacteria and microscopic fungi and examined the growth of Aspergillus niger, Trichoderma sp. and Fusarium oxysporum on peptone-glucose agar containing herbicides.
During the small plot experiments, soil samples were collected 3 times a year from 2-20 cm depth. The total numbers of bacteria and microscopic fungi were determined by plate dilution method, while the method of most probable number (Pochon method) was used to determine the numbers of nitrifying bacteria and cellulose decomposing bacteria. To evaluate the microbiological activity of the soil samples we measured carbon-dioxide release (after 10 days incubation), nitrate production (after 14 days incubation) and the concentration of C and N in the biomass.
We can summarize our results as follows:
• In laboratory experiments, herbicides caused a decrease in the number of bacteria and inhibited the growth of microscopic fungi.
• Frontier 900 EC and Acenit A 880 EC had the strongest inhibiting effect on microorganisms.
• In small plot experiments, herbicide treatment decreased the total number of bacteria and microscopic fungi.
• Herbicides caused a significant increase in the number of nitrifying and cellulose decomposing bacteria.
• Different herbicides containing the same active compound had similar influences on soil microoorganisms.
• A significant increase was observed in the physiological processes of tolerant microorganisms surviving the effects of herbicides -
Microbiological and Chemical Characterization of Different Composts
106-111Views:161Composting of agricultural waste is considered particularly important from the point-of-view of environmental protection. Degradation of organic substance results in a significant reduction of waste volume.
The end product of the composting process, mature compost, can be used as soil coverage against excess loss of wastes, for mulching, for organic manure etc. The problem of composting has come into limelight in environmental studies and in agriculture.
The quality of the mature compost is determined by physical, chemical and biological parameters of the composting process which, in turn, depend on initial composition of the raw materials, the technology, e.g. regular mixing and moistening and on environmental factors. Quality is the key question in compost use.
We studied the composting process in compost windrows of different raw material composition. We measured temperature, humidity content, pH, organic substance content, nitrogen and carbon content.
We counted the number of bacteria, microscopic fungy, ammonifying and cellulose decomposing microorganisms. We directed the composting process with turning weekly (to provide oxygen) and watering (to provide humidity content 40-60%).
We set up windrows of 1 m3 volume from dry plant substances (cornstalk, pea straw, tomato stalk and crop, weeds) and cow manure not older than 1 week. The cow manure was used at ratios of 0%, 35%, 50%, 65% and 100%, respectively.
We measured changes in compost temperature relationship with outside temperature until they were almoust the same. Humidity was 40-60% in most cases.
At the beginning of the process, pH was slightly acidic-neutral; it later becomes neutral-slightly alkaline (pH: 6.93-8.02) as ammonia is liberated from proteins.
At the end of the process, pH decreased again, due to humification.
Organic substance content decreased as microorganisms mineralized them. Organic carbon content decreased gradually due to microorganisms used it as an energy.
Total nitrogen content increased until middle of july and decreased gradually until than.
The carbon/nitrogen rate were higher in the beginning, it decreased until july-august and increased by smaller degree until end of the process.
The number of bacteria was higher in the first three weeks and between june-september. The number of cellulose degrading bacteria was the highest in the first three month, the number of ammonifying bacteria was the highest from the end of may until sepember.
The number of microscopic fungy was significant in the second part of process, after july. -
Effects of cultivation methods on some soil biological parameters of a meadow chernozem soil (Vertisols)
61-66Views:173The effect of extended drought conditions on soil, the unfavourable cultivation technologies and the application of chemicals have been enhancing the processes of physical and biological soil degradation, so the fertility of soil is gradually declining.
The effects of two cultivation methods – traditional ploughing (TP) and conservation tillage (CT) – on the biological activity of a meadow
chernozem soil were examined in a long term experiment. Different parameters of the biological activity of soil were determined. These are
the numbers of total bacteria, microscopic fungi, aerobic cellulose decomposing bacteria, as well as the activities of some important soil
enzymes and CO2 production.
Conservation tillage seemed to be a more favourable cultivation method for the majority of microorganisms, the activities of urease and
dehydrogenase enzymes and CO2 production, compared to the traditional ploughing system. These parameters increased significantly,
especially in the upper layer of conservation tillage plots. Concerning the plant cultures, the majority of microbiological parameters were
higher in the soil of vetch (Vicia sativa L.) depending on the cultivation methods, so involving the pulses to the crop-rotation seems to be
very important in this soil type.
According to the ninth year’s results, the importance of conservation tillage as a means of protecting the soil biological activity in meadow
chernozem (Vertisols) can be established; it was proven by microbiological investigations. -
The application of bentonite and zeolite for soil amelioration in acidic sandy soil
131-137Views:356In a pot experiment, we have studied the effect of bentonite and zeolite in different dosages [control; 5; 10; 15; 20 g kg-1] on acidic (pHH2O=5.65) humus sandy soil. The experiment was set up in 2007 and 2008 in the greenhouse of the UD CASE Department of Agrochemistry and Soil Science. As a test plant, perennial ryegrass (Lolium perenne L.) was used.
In laboratory examinations, pH(H2O), pH(KCl), hidrolytic acidity, nitrate-N content, readily available phosphorus and potassium content were determined. Among soil microbial parameters, the total number of bacteria, the cellulose-decomposing bacteria, the carbon-dioxide production, the microbial biomass-C content of soil, and the saccharase enzyme activity were measured. In the experiment the biomass of the test plant was determined.
The effect of bentonite and zeolite in different dosages can be summarized as follows:
− The pH increased under the effect of low dosages. With the increasing of the pH the hydrolytic acidity - at the bentonite treatments significantly – decreased.
− Regarding the readily available nutrient content of the soil, low and medium dosages proved to be effective. High dosages of bentonite treatments reduced the nitrate-N content, the readily available phosphorus, and potassium content of soil, by zeolite treatments the high dosages reduced the nitrate-N content of soil.
− Regarding the measured soil microbial parameters in both treatments low and medium dosages proved to be also effective, but the high dosages didn’t cause decreasing at the total number of bacteria, and by zeolite treatments the biomass-C content of soil.
− Also the bentonite and zeolite treatments enlarged the biomass of the test plant. We experienced significant increasing by bentonite treatments by the effect of medium and high dosages, while in zeolite treatments only the high dosage caused significantly increasing in plant biomass. The largest dosages decrease the plant biomass.
− Under the statistical analysis we found many medium and tight correlation between the studied parameters. -
Plant production possibilities on a heavy metal contaminated soil with the purpose of biorefinery
215-222Views:257Significant part of not cultivated area of Hungary is not suitable for agricultural utilization because of industrial
pollution. Technologies of biorefinery make reutilization of contaminated areas possible. Biomass of plants
produced on polluted soils can be raw material of valuable products. Applicability of biorefinery was tested on a
heavy metal polluted soil, where the contamination originated from previous mining activity. Complete biomass
utilization was aimed to obtain cosmetic ingredients, pharmaceutical agents, and precursors. During our research
work 88 plant species and varieties were produced and tested for potential utilizable components. Levels of
possible contaminants in these plants were monitored, and amounts of carbohydrates, protein, organic acid and
cellulose were determined as well. Different plant extracts were tested as potential sources of biologically effective
components or as raw materials for lactic acid fermentation. Our results show that biorefinery is a real possibility
for utilization of polluted areas. Numerous plants could be cultivated on contaminated areas without increased
levels of contaminants in their tissues, thus they can be sources of valuable compounds. -
Evaluation some important microbiological parameters of the carbon cycle in chernozem soils profiles
33-39Views:415Some chemical and microbiological properties of the carbon cycle were investigated in three chernozem soil profiles. The soil profiles originated from a long term fertilization experiment (potato) of the University of Debrecen, Látókép, Kryvyi Rig Botanic Garden (grassland) and a large-scale farm (sunflower) of Ukraine. The results of the organic C-content, total number of bacteria, microscopical fungi, cellulose decomposing bacteria, CO2-production, microbial biomass carbon and saccharase and dehydrogenase activities were compared and evaluated with the help of correlation analyses. Close correlation was found between the organic carbon content and the number of microscopical fungi,, saccharase and dehydrogenase enzymes’ activities, as well as close correlation was found between the dehydrogenase activity and microbial biomass-C and saccharase activity.