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Development of an InDel marker set to establish hybridization between wild boar and domestic pig (Sus scrofa) breeds
21-25Views:534Wild boar and domestic pig breeds belong to the same species (Sus scrofa), so they can easily have viable offspring. This could be a problem in preserving the genetic lines of wild boars, keeping clean the food industry from lower-grade hybrid boar meat, and „producing” ethically questionable trophies, too. The aim of our study was to develop a cost-efficient, fast, easy and accurate marker set which can separate the wild boars from hybrids and domestic pig breeds.
The InDel markers were developed using 59 full pig genomes of 17 different breeds (e.g. Duroc, Large White, Landrace, Mangalica, wild boar). Sequence differences between the genomes of wild boars and domestic breeds were identified in variant call files, and verified using the IGV software. Wild boar, mangalica and duroc specific primers to amplify the chosen InDel regions were designed using Primer3.
After preliminary tests five markers were chosen, three wild boar specific, one Mangalica specific and one Duroc specific one. Fluorescently labelled primers were used to make the valuation easier and more accurate with capillary electrophoresis instead of gel-electrophoresis. The markers were optimised individually and in multiplex conditions and tested in samples of 11 breeds.
In conclusion, a new, faster and cheaper set was developed to separate the wild boars from the hybrids and domestic breeds. Based on the preliminary testing on wild boars, duroc and mangalica breeds zero samples resulted false negative, so it is 100% accurate. In addition, it is a much more cost- and time-effective way than testing every single sample with STR sets.
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Estimation of direct and maternal genetic parameters for weaning weight in Hungarian Simmental cattle
17-22Views:364The aim of the current research was to estimate variance components and genetic parameters of weaning weight in Hungarian Simmental cattle. Weaning weight records were obtained from the Association of Hungarian Simmental Breeders. The dataset comprised of 44,278 animals born from 1975 to 2020. The data was analyzed using the restricted maximum likelihood methodology of the Wombat software. We fitted a total of six models to the weaning weight data of Hungarian Simmental cattle. Models ranged from a simple model with animals as the only random effect to a model that had maternal environmental effects as additional random effects as well as direct maternal genetic covariance. Fixed effects in the model comprised of herd, birth year, calving order and sex. Likelihood ratio test was used to determine the best fit model for the data. Results indicated that allowing for direct-maternal genetic covariance increases the direct and maternal effect dramatically. The best fit model had direct and maternal genetic effects as the only random effect with non-zero direct-maternal genetic correlation. Direct heritability, maternal heritability and direct maternal correlation of the best fit model was 0.57, 0.16 and -0.78 respectively. The result indicates that problem of (co-)sampling variation occurs when attempting to partition additive genetic variance into direct and maternal components.
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Using of e-learning in agricultural training programs
117-124Views:165We use the Moodle at the University of Debrecen, Businessand Agricultural Department since January 2007. The Moodle is an open source Learning Management System. Learning Management System (or LMS) which is a software package, which enables the management and delivery of learning content and resources to students. Most LMS systems are web-based to facilitate „anytime, anywhere” access to learning content and administration. LMS tracks student progress in a course and indicates completions. At the least, learning management systems
track individual student progress, record scores of quizzes and tests within an online learning program, and track course completions. The Moodle has more and more function at our Department in education. In January we worked to introduce Moodle in our Faculty. Therefore, we took lessons for the tutors about the usage of the Moodle. Our aim was to develop such a learning system, which is an integral part of educational process,
to improve the efficiency of agricultural higher education. -
Using integrated remote sensing methods in the Nagyerdő Natura 2000 area
19-24Views:458The more widely use of GIS, remote sensing technology provides appropriate data acquisition and data processing tools to build several national and international biodiversity monitoring system of environmental protection and natur conservation. The ChangeHabitats 2 is a similar international project, which uses airborne hyperspectral and airborne laser scanning (airborne LiDAR) sources beyond traditional data collection methods to build a monitoring system of Natura 2000 habitats. The goal of our research, on one hand, was to separate the most typical species of trees which can be found in the largest coverage in the research plots of Debreceni Nagyerdő Nature Reserve from field and airborne remote sensing data, use image classification that based on spectral and geometry (height) characteristics of the trees. On the other hand our goal was to evaluate the efficient use of the integration of mobilGIS, airborne hyperspectral and airborne LiDAR data collecting methods to complement or substitut of the traditional, field data collecting methods. We used ArcGIS 10.2 and Exelis 5.0 GIS software for data evaluation, in which the mosaicing, the selection of plots and the spectral image processing were carried out.
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Evaluation of Hungarian show-jumping results using different measurement variables
81-85Views:350The aim of the study was to compare different fitted models for show-jumping results of sporthorses and to estimate heritability and repeatability value. Show-jumping competition results collected between 1996 and 2011 were analyzed. The database contained 358 342 starts of 10 199 horses. Identity number, name and gender of the horse, rider, competition year, the level and location of the competition and placing were recorded in the database. To measure performance of horses, placing, number of starters and competition level were used. Competitions were categorized into five groups based on their difficulty level. The used repeatability animal model included fixed effects for age, gender, competition place, year of competition (and competition level in case of non-weighted measurement variables), and random effects for rider, animal and permanent environment effect. Variance components were estimated with VCE-6 software package. The goodness-of-fit of the models was low and moderate. Heritability and repeatability values were low for each measurement variables. The best goodness-of-fit model the weighted square root of placing resulted the highest heritability and repeatability value h2=0.074 and R=0.296.
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Evaluation of Hungarian Sporthorse mare performance tests
83-87Views:341Results of the Hungarian Sporthorse mare performance tests were evaluated. Data from the period of 1993-2009 were used, covering
scores of 618 3-year-old and 310 4-year-old mares, 109 of them were tested at both ages. Seventeen traits were scored on the tests, which
covered ten conformational, three free jumping performance and four movement analyses traits, respectively. Breeding value estimation was
based on BLUP animal model. Test year, age and owner were included in the model as fixed effects. Variance components were estimated
with VCE-6 software package. Heritabilities ranged from 0.32 (frame) to 0.50 (saddle region) for conformation traits, from 0.39 (jumping
style) to 0.49 (jumping ability and jumping skill) for free jumping traits and from 0.20 (walk) to 0.48 (canter) for movement analysis traits.
Breeding value indexes were constructed for each trait group. Conformation index was computed based on the weighted scores of the
breeding values of conformational traits. The conformational score scales were used as weightings. Free jumping and movement indexes
contain the proper breeding values with equal weights. A total index was also constructed using conformation index, two times the free
jumping index and two times the movement index. Each breeding values and breeding value indexes were presented with the mean 100 and
standard deviation of 20 for the easier understanding. -
Customer Relationship Systems and Business Models
99-106Views:223The object of this study is a comprehensive review of customer relationship management (CRM) systems applied increasingly in several fields of economy. The CRM is not merely a subsequent software technology and application in the field of Business Intelligence, but rather a novel, comprehensive customer-centric approach to an organization’s philosophy in dealing with its customer for the sake of realization of short- and long-range business target contrary to the traditionally widespread productcentric approach. The object of the study, following a short
synopsis of the theoretical and practical aspects of the CRM systems, is to analyse the range of the actual and potential users and to survey the CRM market. It will be analysed the scope of CRM supported business activities and processes, the terms and possible methods of launching and will be outlined a feasible model, too. The aim of conspectus of different CRM strategies (layers) is to reveal the urging need of turning approach in the field of management. -
The effect of genotype and the location of sampling on the mineral content of wool
157-160Views:609Mineral supplementation is very important in high producing farm animals. The estimation of exact mineral intake is very difficult in forage eating animals, like sheep. Accessing of long term mineral status seems to be possible using wool mineral analysis. However several factors can affect the results. Therefore, the aim of this study was to test the effect of breed and sampling location on the mineral content of sheep wool. 20 Dorper and 20 Tsigai sheep were chosen from the same farm. Samples were obtained from 3 locations (withers, side and quarter) and tested for 8 elements: Ca, Mg, Na, Co, Cu, P, S, Se , Zn. The samples were cleaned with ethyl alcohol from organic contamination, then after adding nitric acid were mineral analized using ultrasonic cleaning unit. The samples were analysed with ICP-OES (Perkin-Elmer, Optima 3300 DV). Statistical analyses were carried out by GLM procedure of SAS statistical analyses software. Differences between means were checked with Tukey-test. Significant breed differences were detected in the case of Mg, Na, S, Se in spite of the same feeding regime. The wool mineral content were within the reference range. The sampling location had no effect on the mineral content of wool.
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Rural Entertainment in the Püspökladány sub-region
110-117Views:147The Püspökladány statistical sub-region is geographically located in Hajdú-Bihar county’s south-west corner, in the area called Nagy-Sárrét. The tourism of the sub-region has been highly determined by the proximity of Hortobágy National Park and the presence of thermal water, one of the most important resources of the region. As Hajdú-Bihar county – especially Debrecen, Hajdúszoboszló and Hortobágy – possesses these attractive factors as well, in the Püspökladány sub-region rural tourism could be an important means of catching the interests of – mostly Hungarian – tourists.
In the course of this research, my objectives were to introduce the rural hosts of the sub-region, the main features and problems of rural hospitality as an enterprise and the characteristics and activities of rural tourist – from the view of the hosts.
I chose questionnaires as the method of the investigation, and evaluated them with the help of SPSS and Microsoft Excel software. During the evaluation, I calculated distribution, arithmetic mean, deviation and carried out significance-analysis.
The investigations confirmed my presumption, i.e. rural tourism has not gained ground yet in the Püspökladány sub-region. This statement is proved by the fact that I completed only 8 questionnaires in a sub-region with 13 settlements, where rural host activity concentrates on 3 settlements. The results of the questionnaire-evaluation prove the aforesaid. -
Applicability of precision weed detection technologies
163-168Views:458In an agricultural field or horticultural plantation, weeds compete with cultivated plants for water and nutrients. The transpirated water by the weeds is needed to be replaced, which saddles surplus costs on the farmer, which could reduce the profitability of crop production. The aim of the precision plant protection system is to protect cultivated plants by applying site-specific technologies and optimized herbicides combination and methods, without environmental damage. The first step of precision weed control is the scouting for weeds. Traditional and modern (passive and active remote sensing) weed surveying technologies are available to detect weeds. The examination was carried out in an intensive apple orchard with drip irrigation system, protected by hail net of the Study and Regional Research Farm of the University of Debrecen near Pallag. The spectral-based weed detection was worked out by the Tetracam ADC broadband multispectral camera and the GreenSeeker 505 vegetation indexmeter. A strong correlation observed between vegetation indices and weed coverage. Based on the collected data, weed maps are created in appropriate software environment, thus the spatial distributions of the weeds are determined. The species level discrimination and the recognition of weed structural parameters were executed based on the 3D point cloud data by Leica ScanStation C10 laser scanner.
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Flesh firmness examination of scab-resistant apple varieties in a storage experiment
93-98Views:291Of the different physical characteristics of fruits, one of the most essential qualities is flesh firmness, as is an important parameter when selling fresh apples, processing the fruit (processing industry) and during its storage. In the scope of our research, we examined five apple varieties originating from the Derecske horticultural site of KITE cPlc. in 2016. The plantation was planted to be suitable for intensive apple production. Samples were collected at the time of maturity (August-October) of the given variety. The five examined apple varieties (Gaia, Isaaq, Modí, Smeralda and Fujion) are all resistant to apple scab (fungal disease caused by Venturia inaequalis). The two-month storage experiment was conducted at 16-17 °C, which had an aggressive effect on our stored apples. Flesh firmness, weight and diameter were measured on three dates for each of the five apple varieties. Our results were evaluated using IBM SPSS Statistics 20 software. We aimed to compare flesh firmness, weight and diameter of the five apple varieties and their changes during storage. Isaaq (86.34 N/cm2), Modí (94.06 N/cm2) and Fujion (84.90 N/cm2) had outstanding initial flesh firmness results. The Modí apple variety provided the best storage parameters.