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Sequence stability at SSR, ISSR and mtDNA loci of common millet (Panicum miliaceum) from the middle ages
10-19Views:321Seed remains of medieval millet, recovered from a 15th century layer (King’s Palace, Budapest, Hungary), showed reddish yellow grain color after rehydrating on tissue culture medium that was close to grain color of modern cultivar Omszkoje. aDNA of medieval c. millet was extracted successfully, analyzed and compared to modern common millets by ISSR, SSR, CAPS and mtDNA. Analyses of fragments and sequences revealed
polymorphism at seven ISSR loci (22 alleles) and at the 5S-18S rDNA locus of mtDNA. CAPS analysis of the 5S-18S rDNA fragment revealed no SNPs in the restriction sites of six endonucleases TaqI, BsuRI, HinfI, MboI, AluI and RsaI. Sequence alignments of the restriction fragments RsaI also revealed
consensus sequence in the medieval sample compared to a modern variety. Morphological characterization of twenty common millet (Panicum miliaceum L., 2n=4×=36) cultivars and landraces revealed four distinct clusters which were apparently consistent with the grain colors of black, black and brown, red, yellow, and white. In the comparative AFLP, SSR and mtDNA analysis modern millet cv. ‘Topáz’ was used. AFLP analysis revealed that extensive DNA degradation had occurred in the 4th CENT. ancient millet resulting in only 2 (1.2%) AFLP fragments (98.8% degradation),
compared to the 15th CENT. medieval millet with 158 (40%) fragments (60% degradation) and modern millet cv. ‘Topáz’ with 264 fragments (100%). Eight AFLP fragments were sequenced after reamplification and cloning. Microsatellite (SSR) analysis at the nuclear gln4, sh1, rps28 and rps15 loci of the medieval DNA revealed one SNP (single nucleotide polymorphism) at the 29th position (A to G) of rps28 locus compared to modern millet.
Mitochondrial (mtDNA) fragment (MboI) amplified at the 5S-18S-rDNA locus in the medieval millet showed no molecular changes compared to modern millet. The results underline the significance of survived aDNA extraction and analysis of excavated seeds for comparative analysis and molecular reconstruction of ancient and extinct plant genotypes. An attempted phenotype reconstruction indicated that medieval common millet showed the closest morphological similarity to modern millet cultivar Omszkoje. -
Fast neutron irradiation long term effect on Ocimum basilicum germination and initial vegetative parameters
23-31Views:231The goal of this study is to assess the long-term (3-year) effects of rapid neutron irradiation using an Americium-Beryllium source on two generations (M0 and M1) of Ocimum basilicum commercial trait. Seeds of each variety and generation were irradiated with 0, 7.5, 20, 40, and 50 Gray in 2018, and then seeded in 2021 – with their corresponding controls – to see how the different irradiation treatments affected germination and early vegetative metrics three years after irradiation. In the first generation, no significant changes between the treatments were seen. Increased irradiation dosage resulted in a significant decrease in germination % in the second generation (M1), but a significant increase in seedling height. The M0 generation seedlings, on the other hand, were substantially shorter than controls. In the M0 generation, the higher dosages (40 and 50 Gray) were deadly, and seedlings were terminated 7–10 days after germination. The irradiation dose of 20 Gray was shown to be the most successful in inducing viable and beneficial alterations for phenotypic characteristics in basil. When compared to control plants, the lower (7.5 and 20 Gray) bombardment generated leaf anomalies such as smaller leaves and internodes, a split apex, and a ribbon-like stem.
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Molecular Biological Approach of Crocus sativus L. and its Allies
112-115Views:616The hay saffron (Crocus sativus L.) is a sterile triploid plant, known in human culture only, with no fertile seeds produced. The origin of saffron is still a mist, however it is assumed to be an autopoliploid mutant or a hybrid. The recent classification and most of the former taxonomic publications define C. sativus to be derived from C. cartwrightianus, a wild species. Because of the sterility of hay saffron it seemed to be reasonable to apply molecular biological methods to complete classical taxonomic studies in examining its relations. The DNA polymorphism based AFLP method has confirmed the close relationship between these species.
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Impact of precision irrigation on the unit income of maize production
157-162Views:321The study of the economic/economic impact of precision farming should be a priority area in digital agriculture, as the results, profitability, and efficiency indicators can have a significant decision-support effect on the development of both the agronomic and the technical regions of individual farms both in the longer and shorter term. Individual firms, companies, farmers, and family farms quantify the effectiveness of their farming processes. The modern age offers the possibility of digitally recording all the elements of farming technology, making it possible to analyse the cost-effectiveness of a farm more effectively and, in some cases, to carry out more detailed analyses. Nevertheless, the number of farms demonstrating their profitability with such precise economic calculations is still minimal.
Our analyses were conducted on a 56,02 ha field of Balogh Farm-Tépe Ltd. The agricultural operations carried out were fully documented so that the inputs (seeds, fertilisers, pesticides, crop enhancers) were recorded in coordinates and kind, as well as the specific yields, grain moisture data, irrigation norms, and irrigation rotations. At the same time, the company's owner provided the data's monetary value. The main econometric indicators (yield, production value, cost of production, income, cost price) related to the evaluation of the enterprise management were evaluated along with the spatial data in the irrigated and non-irrigated tables. Our calculations show that a given year's climatic and market characteristics fundamentally determine the cost and income relations of a plot of land (and thus of an entire farm). In addition to additional inputs, introducing some elements of precision farming and intensification and increasing yields improves yield security and allows for excellent yield stability.
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Essential oil extraction from herbs and their use in the food industry
59-74Views:588Essential oil extraction of wild caraway and thyme was performed using a classical (HD) and microwave hydro-distillation (MWHD) and a laboratory supercritical fluid extraction (SFE) with a carbon dioxide as solvent. Our experiments demonstrated that the extraction yield of the essential oil performed in same conditions was influenced by the location of growth area; the maximum extraction yield of 10 ml 100 g-1 caraway was obtained from dried seeds collected from Csíkmadaras. This quantity far exceeded the yield of the Újtusnád samples. In the case of wild caraway (Carum carvi L.), the extraction method influenced thecomposition of the essential oil (carvone/limonene ratio), the highest limonene content being achieved by classical hydro-distillation. In the case of thyme, this effect was not detected, the thymol/carvacrol ratio was independent from the given extraction method. The obtained thyme essential oil possesses high antimicrobial activity demonstrated by agar diffusion test. The thyme extract provides a good protection against microorganisms collected on the surface of fresh vegetables following bacterial stains: Citrobacter portucalensis, Pseudomonas hunanensis, Pseudomonas baetica, Pseudomonas parafulva, Bacillus mojavensis and Enterobacter cloacae. Protective effect was also detected on the vegetable surface of by chitosan-based edible film coating during a 6-day-long storage period at a temperature of 4 °C. The caraway essential oil used as soft cheese seasoning with a direct, dilution-free method, proved to be unsuitable because the uneven distribution and confer a strong, unpleasant taste to the product in comparison with the ground wild caraway seed-dressed cheese.
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Soil analysis as the foundation of precision nutritive supply in the Hajdúszoboszló region
141-148Views:282Larger cultivated plots are heterogeneous from a pedological aspect. Heterogeneity causes problems during fertilization and harvest. The heterogeneity of cultivated areas can be compensated by fertilization which is based on soil analysis. We carried out research into the changes of the soil on three soil types, from 1966 to 2006, on the cultivated areas of Hajdúszoboszló.
There were no significant changes in pH on chernozem meadow soil and meadow chernozem soil, but the pH increased in 0-30 cm layer on type meadow solonetz soil. The saline content decreased in every examined soil type. Decrease was the largest on meadow solonetz soil. Decline of humus content was the largest (0.95%) on chernozem meadow soil, and the smallest (0.39%) on meadow chernozem soil. The nitrogen content decreased with 528 ppm in the 0-30 cm layer on chernozem meadow soil, and decreased by 186 ppm on meadow solonetz soil. Phosphorus and
potassium content increased in every examined soil types. Rise of phosphorus content was 188.9 ppm in the 0-30 cm layer on meadow chernozem soil. The potassium content rose by 153.7 ppm on this soil type. Phosphorus content increased with 70.8 ppm, and potassium content increased by 57.6 ppm from 1966 to 2006.