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Effect of Soil Covering on the Soil Enzyme Activity of Integrated Orchard
20-29Views:88The purpose of our experiments is to discover the effect of different soil cover matters (agrofoil and black polyethylene) on the activity of some enzymes (phosphatase, saccharase, urease, catalase, dehydrogenase) occuring in soil. Soil samples were taken from a cider apple plantation of the Fruit Producing Research and Advisory Kht Újfehértó. The enzyme activity was measured according to Krámer and Erdei (1959a), Kuprevič and Tsherbakova (1956), Kuprevič et al. (1966), Frankenberger and Johanson (1983), Mersi and Schinner (1991). Soil moisture content was by conventional (drying chamber) method measured during every sampling and enzyme activity was transpolated to absolute dry soil. Results were estimated by mathematical methods (variation analysis, correlation counting). Soil samples were taken by trials 5 times (in every two months) a year in the vegetation period from March to November.
By recording the monthly changes of the enzyme activity we have observed the following. The activity of the phosphatase was generally the highest in May and the lowest in November. Depending on the trials, high values were also measured in March and September. The activity of the saccharase was generally the highest in November and the lowest in June, but at the same time peaks even occured in May and September. The highest urease avtivity was measured in September and November, and the lowest activity in May and July, also depending on the trials. In the year 2000, after a deep point in March, the activity of the catalase was the highest in November or by certain trials in September. In 2001, the lowest activity was also measured in March, but the highest activity appeared in November in case of one-minute trial, and in May in consequence of two-minute trial. Finally the activity of dehydrogenase was the highest in November and the lowest in July apart from the model years.
There were essential differences in rainfall of the two experimental years which was reflected in the enzyme activities. There was a poor positive significant relationship between soil moisture content and enzyme activity values in case of phosphatase, saccharase, urease (r=0,426; 0,480; 0,396) respectively. In case of catalase1 (r=0,518), catalase (r=0,556), dehydrogenase (r=0,559) we obtained a medium strong positive relationship between soil moisture content and enzyme activity values. By evaluating the effect of different trials in case of every examined enzyme significantly higher values were detected in soils covered by agrofoil (a porous black polyethylene) than in soils covered by black polyethylene or in uncovered soils. Moreover, the soil covered by black polyethylene showed significantly higher enzyme activities (besides phosphatase) than the control soil. Thus soil-covering meant statistically significant advantages in enzyme activity as opposed to uncovered soil proved. -
The variation of breast milk composition during the lactation stage for mothers from the Csík region
93-98Views:98The aim of our research was the examination of breast-milk composition from mothers living in the Csík region and to follow their milk composition variations during lactation. In this article we present the results obtained from three mothers, paying particular attention to essential components. The breast milk samples were collected from healthy mothers with similar habits and age. The milk samples were collected with a hand pump at the
same time after the feeding. The sampling period was from day 5 to the 14th weeks of lactation. The nutrition of mothers was recorded on a questionnaire, completed by the mothers themselves. Comparing our experimental results with data in the literature it was concluded that the milk protein content was very similar to the milk of mothers from other European countries, and is decreased during lactation.
The total saturated fatty acid content was lower, but the palmitic acid content was slightly higher. Regarding the essential fatty acid composition the linoleic- and the arachidonic acid contents were appropriate from a nutritional point of view. The linolenic acid was present in lower amounts, but the docosahexanoic acid was almost undetectable.
The iron and manganese contents were in good agreemen with published results, but the zinc content of the breast milk was lower and its copper content was higher. These differences in milk composition can be explained by the different nutritional habits of our subjects. -
Data on the bumblebee assemblages (Apidae: Bombus spp.) lives in lands under agri-environment commitment
31-35Views:220The goal of agri-environmental schemes (AES) and greening programs are protecting and increasing biodiversity in agricultural lands. The evaluation of effectiveness of AES needs further investigations. For the purpose of investigations, species and species groups should be selected which can indicate the effects of changes in landscape use on biodiversity. Bumblebees are good indicators for this purpose.
The role of bumblebees in pollination is well studied but in the case of different crops, much less detailed data are available. In 2018, bumblebee assemblages of 44 sites belonged to 8 different agricultural and semi-natural habitat types were studied in the surroundings of Sajószöged, Tiszaújváros and Derecske.
This study provides new distribution data of 8 bumblebee species in three 10×10 km UTM cells covering the sampling area. According to our results, the alfalfa and red clover fields and semi-natural grasslands has more species rich and abundant bumblebee assemblages than different crop fields (sunflower, oilseed radish and vegetable morrow) and can help protect bumblebee assemblages of agricultural lands. Based on the collected distribution and abundance data, the role of the bumblebees in pollination of the studied crops should be re-evaluated.
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The effect of wool staple length on the mineral content using the same sheep feeding regime
97-100Views:262The wool of sheep is suitable to test the mineral supply; however, a number of factors could affect the results. The growth rate and length of staple can be very different according to season, physiological state and individual variation. These factors are likely to affect the quantity of minerals accumulated into the wool. Therefore, the purpose of this research was to examine whether there is a difference between the mineral content of wool nearly reached the full staple length and the freshly grown wool using the same feeding regime.
10 Tsigai pregnant sheep have been selected randomly from the same farm. Wool samples were obtained from the withers, side and quarter with bended scissors. Samples were mineralized using nitric acid and hydrogen peroxide using ultrasonic cleaning unit. P, Ca, Mg, Na, S, Cu,Se and Zn content were determined by ICP-OES. Statistical analyses were carried out by SAS (SAS Institute Inc., Cary, NC) GLM procedure.Differences between means were tested by Tukey test. Significantly lower Ca, Na, P, Zn values were found in case of intensively grown wool. Sampling location did not affect the mineral content. Herd mineral supply was adequate. Our results suggest that intensively grown wool samples have to be used for mineral analyses. -
Chromatometric comparison of Eurasian Collared Dove (Streptopelia decaocto) and Feral Pigeon (Columba livia domestica) feathers
19-22Views:194Chromatometric examination of the plumage of birds is a poorly researched topic. We have approached this issue in primarily aspect of differences in plumage of species. Moulted feathers sample collection method has been increasingly used. Reliable identification of feathers becomes an increasingly important issue, hence need for an exact measurement-based methodology. Eurasian Collared Dove (Streptopelia decaocto) and Feral Pigeon (Columba livia domestica) primary, secondary and tail feathers were studied. Chromatometric parameters of feathers were measured in CIELAB color system and then statistical analysis (Independent samples t-test, Descriptive Statistics, Discriminant Analysis) was performed to compare the two species. Instrumental measurements has been confirmed the high similarity between colors of the two species, however species specific differences were also found. Lightless (L*) value were significantly characteristic of particular species, while the red/green (a*) and yellow/blue (b*) value had lower Predictive Power. We identified feathers and the variables which useable to separate the two species and determined the associated Confidence Intervals of these values. Our results may draw attention to a new potential direction for exact identification of the moulted feathers during sample collection.
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Changes in the Composition of the Milk of Racka Sheep During Lactation
24-28Views:123We examined the changes in the composition of the milk of 36 Racka sheep during lactation. As milking of Racka sheep is not performed anywhere in the country, the test was restricted to the examination of milk components. However, quantity could not be measured. We analysed the fat, protein, lactose, carbamide and somatic cell count content of the milk from sampling. By means of correlation analysis, milk components were compared to the age of ewes, then the number of lactation days. With the advancing of the lactation and by analysing the milk components, we found differences between the two colour-variants of Racka sheep. Additional trials are required to decide whether the differences between the two colour-variants may be considered as constants, or can be attributable to the relatively small number of samples.
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Storage of ram semen in gelatin supplemented extender
7-10Views:130The aim of our study was to examine how different gelatin concentrations affect ram semens viability in liquid storage at 5 oC for five days. Our hypothesis was if we add gelatin to the semen extender, than the viability of ram semen will be better in the extenders containing gelatin, than the control. We used two different semen extenders:1.5% UHT milk and 1.5% UHT milk + 5% egg yolk. We added 0; 0.5; 1.0; 1.5; 2.0% Dr. Oetker gelatin to the semen extenders. We stored the semen for five days at 5 oC and in every 24 hour we made sampling.
We stained the smears with Kovács-Foote staining and evaluated them with light-microscope. We categorized the cells in five groups like: live and intact cells, live cells with injured acrosome, dead cells, live head with dead tail and live tail with dead head. We used one-way analysis of variance (ANOVA) to assign how gelatin concentration affects the number of the categorized cells. On the fifth day, the viability was the best in the following semen extenders: 1.5% fat UHT milk + 1.0% gelatin and 1.5% fat UHT milk + 1.5% gelatin, but it was not significant (p>0.05). -
Nitrogen Content of Hungarian Soils and Nitrogen Fertilization
51-61Views:100For crop production and agricultural production, the most important natural resource is the soil that can optionally renew. Paralelly with this, soil plays a major role in the geological and biological cycle of elements. As a result of the big (geological) and small (biological) element cycles, the elements and combines neccessary for organisms can accumulate in the soil creating suitable living conditions for plants and other organisms. Soil is a heterogenous system both horizontally and vertically, and soil constituents show great variety in all the three dimensions, in addition, most of the parameters can also change between two examination dates. When talking about the factors influencing plant production, one should take into account this variation and heterogenity in time and space. When making fertilization recommendations, these factors should all be considered. In any consultation system, most of the mistakes and errors made are due to the unsatisfying soil testing and the negligence of soil heterogenity. In the practice of fertilization the biggest mistake is the improper soil sampling, then comes the methodical mistake of soil testing, which is followed by the inaccuracy of instrumental analysis and the subjectivity of result evaluation, but the latter two are negligible compared to the others. Under normal, i.e. production conditions, the quantity and distribution of nutrients in the soil are greatly dependent upon the applied technology, the amount and form of the applied natural and artificial fertilizers and the quality of fertilization.
Fertilization recommendations are needed because in the layer which is accessible for plant roots only a part of the nutrient content is available for plants in a specific production cycle. An illustration of this is that though the upper 1 m layer of an average chernozem soil contains more than 5000 kg N, 12000 kg K2O and 1500 kg P2O5 (form of expression mostly used in Hungary), the application of fertilizer doses which are just fractions of these quantities is essential. This is due to the fact that the available amount of the total nutrient content depends from the quality of soil, the environmental factors (the physical and chemical qualities of the soil) and the specific nutrient’s qualities (solubility, adsorption). Knowledge of these processes and the examination of the factors influencing the actual nutrient content are vital for working out a fertilization practice, which does not put more strain on the environment than neccessary.All of the above mentioned should be considered when applying inputs in the fields. In a well-functioning practice that considers the economic and environmental conditions (unfortunately the present production and economic conditions do not enable an appropriate level and degree), three nutrients are supplemented generally (and were supplemented in the last decades): nitrogen, phosphorus, potassium.
Studying the nutrient balance of the Hungarian field production’s last hundred years, we can draw some interesting conclusions.
The nutrient balance became positive for nitrogen and potassium in the second half of the 1960’s, while for phosphorus it was positive from the first half of the 1960’s and this period lasted until the end of the 1980’s.
Neither before the 1960’s, nor since the 1990’s has the amount of nutrients supplemented in a specific year reached the amount of the nutrient uptake of the same year. -
The effects of the extraction of non-ferrous metals on the ichthiofauna of the Lapus river bassin
6-8Views:77Over the period 2003-2005 we made ichthyologic research in the basin of the Lapus river. During the sampling we noticed that in some area the water was polluted. In those areas fishweretotallyorpartiallymissing.
The main pollution sources are those related to nonferrous metal extraction and processing, but there is also pollution from organic substances resulted from the communal residual waters, as well as the “tuica”(alcoholic drink) distilleries.
Confronting the spots of the pollution sources with the results of the ichthyologic research.we noticed a significantcorrespondencebetween the qualitative and quantitative component of the ichthyofauna and the presence of these sources.
We could, therefore prove the effect of the process of self-purificationofthewater,aswellastheexistenceofsomespeciesoffishshowing a great degree of tolerance towards pollution. -
Data on the Orthoptera fauna of characteristic agricultural landscape in the Carpathian Lowland
25-34Views:167Orthoptera fauna and assemblages of natural and semi-natural grasslands of the Hungarian Lowland are well-known, however, little is known about assemblages living in agricultural and anthropogenic habitats such as arable lands, roadsides, hedges, and riverbanks. Due to climate change, intensification of agriculture, and change of habitat use, these habitat types become increasingly important.
To collect data on these mainly unknown habitat types, a three-year study was carried out on the Orthoptera fauna and assemblages of the firth region of the Tisza and Sajó rivers. This area was mainly unknown, and our research contributes to increasing knowledge and provides a base for further investigations.
In the 40 sampling sites of the studied region, an occurrence of 30 Orthoptera species was recorded based on 2241 sampled individuals. In this study, we provide 540 new distribution data records of orthopterans that means an almost eightfold increase of the known data. Orthoptera assemblages of different agricultural habitat types showed significant differences considering both species richness and composition. Data suggested that non-cultivated habitat patches of dirt-roads, roadsides and stubble fields and even extensively used pastures, hayfields and alfalfa, red clover, and even wheat fields can preserve relatively species-rich Orthoptera assemblages. Contrary weedy sites of these cultivars and intensively used arable lands (maize, sunflower and rape fields) showed extremely low species diversity.
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Stand evaluation, crop estimation and yield analysis of winter wheat for the optimization of yields
103-109Views:130The authors have been carrying out stand evaluation, crop estimation and yield analysis in winter wheat since 2012. The sampling areas were assigned at the fields of the Training Farm of the Faculty of Agricultural and Food Sciences of Széchenyi István University Mosonmagyaróvár according to the structure of the cropping system. According to their observations the value of field emergence is always lower than the laboratory germination. The weak emergence is important because the lower plant density cannot be compensated by the increased tillering in spite of having larger plant growth space. It is proven by the fact that they detected strong productive tillering even at 5 and 10 mm plant spacing while there were single-spiked plants at 40-50 mm plant spacing as well. The analysis revealed that the total ear mass and grain mass of wheat plants bearing two or more ears is almost the double than that of the single-spiked plants. It was a further basic experience that the largest ear of ”multiple-spiked” plants is always heavier than the single ear of one-spiked plants. Plants with intense tillering and more ears demonstrate the importance of proper seedbed preparation and drilling and the significance of sowing good quality seeds. These are the factors that determine field germination and emergence, influence the speed and intensity of initial development and by all these factors the sufficient productive tillering. The authors emphasize the use of exact and objective methods at crop estimation, e.g. the relationship between the ear mass and the yield which is in strong correlation whilst ear length and grain mass are not suitable for a precise estimation. The authors conclude that crop estimation and yield analysis must be inevitable tools of modern crop production and will be particularly important in precision agriculture. These tools also qualify the job done by farmers and helps to identify the areas that require special attention.
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Soil Biological Activity within Integrated and Ecological Management of Soil
47-52Views:83The effects of the integrated (IS) and ecological (ES) management of soil on chosen parameters of soil biological activity were investigated in the period 1999-2000. The following characteristics were determined: biomass of microorganisms (Cmic), dehydrogenase activity (DHA), an amount of potentially mineralizable nitrogen (Nbiol), and nitrification intensity. Soil samples were collected from a stationary field experiment established in 1990 on gley brown soil at the Experimental Station of Slovak Agricultural University, Nitra. For each field with a different crop rotations two fertilization treatments were selected: (a) no fertilization and (b) use of manure for silage maize and, within IS, also mineral fertilizers. There was a statistically significant difference at α = 0.05 in the amount of biologically released nitrogen (Nbiol) between both systems and in the nitrification intensity in favour of ES. A higher amount of microbial biomass (Cmic) was noted for ES but without statistical significance. Cultivated crops and the timing of soil sampling were found to have the greatest effect on all the parameters observed in individual experimental years and within the two systems of soil management.
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Estimating Genetic Parameters using a Random Regression Model
53-55Views:119One of the most important part of the genetic evaluation using a random regression model is the estimation of variance components. This is the topic of many papers because the large computational costs. We can use restricted maximum likelihood (REML), Gibbs sampling and ℜ method for the estimation of genetic parameters. The variance components are necessary to calculate the heritabilities and repeatabilities.
The aim of our paper is to estimate the variance components using a random regression repeatability model from test day data set of Hungarian Holstein-Friesian dairy cows and to analyse the change of additive genetic and permanent environmental variance, heritability and repeatability over lactation. -
Examination of nutrient leaching dynamics of Solidago virgaurea in Hévíz Lake and Hévíz canal
207-211Views:114The different nitrogen and phosphorus elements are prime constituents of the ecosystem and can exert a major effect on aquatic species and the overall ecological cycle. During our investigation we examined the nutrient leaching dynamics in decomposing leaf litter (stem and leaf blades) of Solidago virgaurea. We chose four different sampling sites during the winter period with different water temperatures in the Hévíz Lake and Hévíz canal. The bottles containing the plant material and water were incubated at natural temperatures and the fluid was removed at particular times. Water parameters such as pH level, conductivity, nutrients (ammonium and phosphates) were measured. In the case of Solidago leaf, we obtained higher values for all parameters compared to the stem ones.
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Mitochondrial DNA-based diversity study of Hungarian brown hares (Lepus europaeus Pallas 1778)
23-29Views:153The brown hare being an important game species which is widespread across the European continent has been in focus of many population genetic studies. However only a few comprising researches can be found on the diversity of Central-European populations.
The aim of our large scale long term ongoing study is to fill this gap of information on the species by describing the genetic history and structure of the brown hare populations of the area using both mitochondrial DNA markers and genomic skin and hair colour regulating genes.
This article gives forth a part of our results concerning the mitochondrial DNA diversity of Hungarian brown hares based on amplification of a 512 bp long D-loop sequence. N=39 tissue or hair samples have been collected from 15 sampling sites on the Hungarian Great Plain. We have described a high level of haplotype diversity (Hd=0.879±0.044) based on a 410 bp alignment of our sequences. We have found 17 haplotypes within our sample set with the nucleotid diversity of π=0.01167±0.0022. Our ongoing research shows high genetic diversity for the brown hare in the studied region and a second alignment with 156 sequences downloaded from GenBank indicates a geographic pattern of haplotypes among the studied populations though these results need confirmation by our further analyses.