Search
Search Results
-
Modern methods to determine vitamin E in foods and feeds
53-58Views:104Highly specific and sensitive, the proposed fluorometric method for determining α-tocopherol is robust and fairly fast. It
has been tested in parallel with a conventional thin layer chromatographic method on foods and feeds. The only necessary
cleanup is the usual saponification. The unsaponificable fraction can be extracted with ethyl ether or, preferably, with
Extrelut columns. Reagents and their solvents are added to the isooctane solution before each successive reaction and are
then eliminated by partition with water. The α-tocopherol (α-T) derivative always remains in isooctane. The first step is
nitrosation and elimination of tocopherols and tocotrienols other than α-isomers. α-T is then oxidized to α-tocored (α-TR)
with a mixture of sulfuric acid, ferric chloride, and iodine bromide. Α-TR is then condensed to a new reagent: 4,5-dimethylo-
phenylenediamine. The phenazine formed is strongly fluorescent. Iodine and bromine add to the double bonds of α-
tocotrienol present and quench the fluorescence of its phenazine. -
Influence of H-FABP gene polymorphisms on slaughter value of hybrid pigs
55-60Views:229The H-FABP gene was defined as a potential candidate gene influencing the fat deposition traits, primarily the intramuscular fat content. The aim of this study is to define whether the previously reported gene mutations are connected with the slaughter traits measured in a standard slaughterhouse. The study included data from 405 gilts and barrows from 2 different samples. The two chosen mutation (HFABP1: c. 103 T>C, HFABP2: c. 1970 T>C) were detected in one reaction with PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Lenght Polymorphism) method with HinfI restrictoin enzyme. The allel frequencies are as follows: 103T(H)=0.75; 103C(h)=0.25, 1970T=0.32; 1970C=0.68. A HFABP1 mutation has significant effect on backfat thickness and lean meat % at stable 1 (sample 1), but there were no effect at stable 2 (sample 2). The analysis of values of production traits, depending on HFABP2 genotype did not reveal significant differences. Based on this study we can’t get a clear conclusion on the impact of polymorphisms on production parameters. In the examined flock the allele frequency of mutation in 5 'UTR is identical to the literature data, i. e. the more favorable variant regarding the intramuscular fat content is predominant in the population.
-
The Effect of Sowing Time and Plant Density on the Yield of MaizeHybrids
95-104Views:81The crop technology of maize has two important elements, sowing time and plant density. In 2003 and 2004 we studied the effect of these two factors on the growth and production of maize in an experiment carried out near Hajdúböszörmény.
The soil of the experimental plots was meadow soil.
Weather in both years was differed greatly. 2003 was drought. Neither the distribution nor the quantity of the precipitation were suitable in the growing season for maize. This fact basically determined the results.
In 2004, we could talk about a favorable and rainy season. The distribution and quantity of precipitation was suitable between April and September. The average temperature was also suitable for maize.
Results of the sowing time experiment:
In 2003, we tested seven hybrids at four sowing times. Hybrids in the early maturity group gave the highest yield at the later sowing time, while the hybrids of the long maturity group gave it at the earlier planting time. The yield of PR34B97, PR36N70, PR36M53 hybrids was the best at every planting time. The moisture loss of hybrids in the late maturity group was faster in the maturity season, but the seed moisture content was higher than the hybrids with early sowing time. The seed moisture content was very low due to the droughty year. In two hybrid cases, this value was higher than 20% only at the fourth sowing time.
In 2004, we examined the yield and seed moisture content of nine hybrids. In the favorable crop year, the yield of every hybrid was the highest at the second and third sowing time. Yields of PR34H31 and PR38B85 hybrids were significant. The seed moisture content at harvest was higher than the previous year due to the rainy season. In the case of hybrids sown later, this value was higher by 30%. However, we noticed that this value was lower at the earlier sowing time than at the later.
The crop year had a more dynamic effect on maize than the sowing time. First of all, the quantity and distribution of precipitation played an important role in respect to yield safety.
Results of the plant density experiment:
We tested the reaction of hybrids at four plant densities (45,000, 60,000, 75,000 and 90,000 stock/ha) every two years. In 2003, the tested seven hybrids reached the highest yield at the 90,000 stock/ha in the face of a droughty year. The effect of forecrop and favorable nutrients caused these results. In the rainy 2004 year, the yield grew linear with the growing plant density. The yield of the best hybrids were 14-15 t/ha at the 90,000 stock/ha.
Such a high plant density (90,000 stock/ha) couldn’t adaptable in farm conditions in rainy season. It is practical to determine the interval of plant density besides the optimum plant density of hybrids which gave correct yield. The farmers have to use the low value of this interval due to the frequent of the droughty years. -
Investigation of the Resistance Against the Fusarium solani at Different Pea Varieties and Combinations of Breeding
157-160Views:33The Fusarium species are soil and polyphage parasits, and the rate of damage, caused by them, highly depend on interactions between climatic and edaphic factors and also on sensitivity of cultivars. Even though about 70-80 percent of the widely grown green peas cultivars is resistant to Fusarium oxysporum f. sp. pisi 1. race, the rate of Fusarium infections and severity of symptoms increased in the latest years. It is supposed that another Fusarium sp. the Fusarium solani has been spreading. The most exact way to study the cultivars in a provoking garden established in natural environment, where the pathogen is artificially enriched to a level, at which the cultivars can be distinguished according to their susceptibility. In the provoking garden the reaction against to Fusarium of our breeding lines and our registered cultivars and cultivars existed on the National List (including cultivars with well-known susceptibility as standards) are examined year by year. In our experiments we could found two green peas cultivars to be resistant to Fusarium solani (Early sweet (13,36%) and Lora (16,9%)) The breeding lines Margit and 8607/75-3-2 proved to be the most susceptible to Fusarium solani (94,4% and 73,1% infected plants, respectively).