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Fruit melanotic ringspot (FMRS) – a disease of resistant Capsicum genotypes infected with Tomato spotted wilt virus (TSWV) on the fruits
64-69Views:188Etiology of pepper fruit melanotic ringspot (FMRS) disease (Salamon, 2009) was studied on fruit samples collected in forced pepper populations. It was noticed that in spite of heavy thrips (Frankliniella occidentalis) infestations and of TSWV epidemy detected in the forcing houses, FMRS occurred only in plants having healthy foliage. Symptomatological surveys strongly suggested that FMRS appeared exclusively in specific pepper genotypes. The size of melanotic ringspots has been observed to grow at room temperature during postripening of diseased fruits. A mechanically transmitted plant virus was isolated from symptomatic parts of 9 white pepper fruits affected by FMRS. On test plants each of the virus isolates caused systemic symptoms characteristic to TSWV. Using cDNA/PCR technique and TSWV N-gene specific primers a ca. 300 bp long DNA fragment has been amplified from total nucleic acid extracted from symptomatic tissues but never from asymptomatic parts of the fruits showing FMRS. Plant progenies grown from seeds of FMRS diseased fruits segregated in respect of resistance and/or susceptibility to TSWV infection. TSWV was also detected in and isolated from three fruits showed non-melanotic yellow rings (one of them was infected with a tobamovirus, too). Seedlings derived from these fruits proved to be susceptible to TSWV. Based on the above results we could conclude that the FMRS disease developed on fruits of “cecei” type white peppers that carry a TSWV resistance gene, most likely the Tsw gene in heterozygous form. These fruits were infected with thrips transmitted TSWV and FRMS appeared as a hypersentive reaction (HR) manifested in fruits.
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Impact of fertilization on production capacity and reaction to fertilization in maize hybrids
42-46Views:174New varieties and fertilization have significantly increased yields of maize in recent decades. It has to be taken into account however that the interactions and the balanced combination of cropping factors (ecological, biological and agrotechnical) are the main factors which determine yields. Weather conditions were rather unfavourable during the last decade. Extreme weather conditions occurred due to global warming; 6 of 10 years were plagued by drought. Consequently yields increased from 10-20% to 30-50%. In view of varieties the situation is advantageous, maybe the supply of hybrids is too high, nevertheless those hybrids need to be selected which are particularly well adjusted to the ecological conditions. Many technologies can be applied which vary according to intensity, but the balanced combination of cropping factors should be secured on the basis of the hybrid’s intensity. Among agrotechnical factors the compensation of nutrients and technological conditions were inadequate. These days farmers only use nitrogenous fertilizers consequently they significantly decrease the easily available P and K content of the soil which in long term leads to the deterioration of the soil’s productivity. The technological background is therefore important, because sufficient yields can only be expected if agrotechnical operations are carried out in the right time and quality.
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Technological development of sustainable maize production
83-88Views:322In our research we examined the effect of the hybrid, the nutrient supply, the number of plants and the abiotic factors (temperature, amount of precipitation) on the yield, crop quality and yield stability of maize. We devoted special attention to the natural nutrient utilization ability and fertilizer reaction of maize.
The experiment took place in Hajdúszoboszló on chernozem soil, on a nearly eight ha field. The size of one plot was 206 m2, this it was a half-industrial experiment. We tested six hybrids with different genetic characteristics and growing seasons. I analysed the correlation between the nutrient supply and the yield of maize hybrids with control treatment (treatment without fertilization) and with N 80, P2O5 60, K2O 70 kg ha-1 and N 160, P2O5 120, K2O 140 kg ha-1 fertilizer treatments. Yield increasing effect of the fertilizer also depended on the number of plants per hectare at a great extent. The number of plants of the six tested hybrids was 60, 70, and 80 thousand plants per ha.
In Hajdúszoboszló, in 2016 the amount of rainfall from January to October was 605 mm, which was more than the average of 30 years by 160 mm. The yield of hybrids without fertilization changed between 9.63–11.6 t ha-1 depending on the number of plants.
The six tested hybrids is 10.65 t ha-1 in the average of the stand density of 60, 70 and 80 thousand plants per hectare without fertilization, while it is 12.24 t ha-1 with N80+PK fertilizer treatment. That increase in the yield is 1.6 t ha-1, it is significant.
Da Sonka hybrid is sensitive to weather, it is able to produce 6 t ha-1 additional yield in case of favourable condition. However, it has a low stress tolerance. The most stable yields were observed at Kamaria and Pioneer hybrids. The effect of vintage is also an important factor on the yield. In average, the yield of maize was 6.81 t ha-1 in 2015, which was a drought year and 11.86 t ha-1 in 2016 that was a favourable year. -
The effect of β-glucan, carotenoids, oligosaccharides and anthocyanins on bacteria groups of excreta in broiler chickens
15-20Views:869This study was conducted to examine the effect of natural compounds, such as β-glucan, carotenoids, oligosaccharides, and anthocyanins in the diet on bacteria gropus of excreta in Ross 308 broiler chickens. Chickens were fed 5 diets: control (basal) diet, a diet supplemented by β-glucan at 0.05%, and diets supplemented by carotenoids, oligosaccharides, or anthocyanins at 0.5% of each compound. On experimental day 19, excreta were collected to determine the proportion of Lactobacillus, Bifidobacterium, Campylobacter, Clostridium, and Escherichia coli. Samples were collected aseptically and snap-frozen in liquid nitrogen. Bacterial DNA was isolated from samples, then polymerase chain reaction using primer pairs designed to the 16S rDNA of bacterial groups were applied to define the proportion of the mentioned bacteria. Another universal primer pair was used to amplify a region of 16S rDNA of all the examined bacteria. Proportion of each bacterial groups was determined relatively to the intensity of universal PCR product band by gel documenting system and ImageLab software. Based on the results, carotenoids and anthocyanins increased the proportion of Bifidobacterium, which might imply the beneficial effects of the mentioned compounds on the bacteria composition of excreta.
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Hybrid-specific nutrient and water use of maize on chernozem soil
51-54Views:218The field research was set up on chernozem soil at the Látókép AGTC KIT research area of the University of Debrecen. The study focused on yield, water utilization, nutrient reaction and the amount of yield per kg fertilizer of corn hybrid NX 47279 in 2011 and 2012. Based on the yield results it can be concluded that the largest yield in 2011 was 15 963 kg ha-1 at level N120+PK, while in 2012, the maximum yield amounted to 14 972 kg ha-1 at level N90+PK. Surplus yield per kg fertilizer proved that in 2011 level N30+PK resulted in the highest surplus yield (42.3 kg kg-1) compared to the control treatment. In 2012, yield growth was 18.0 kg kg-1 compared to the control treatment. We measured at level N60+PK 17,5 kg kg-1 compared to at level N30+PK, at the N90+PK 17,7 kg kg-1 compared to at level N60+PK. level N30+PK kg kg-1, 17.5 kg kg-1 at level N60+PK and 17.7 kg kg-1 at level N90+PK compared to the control treatment.
Results of the regression analysis showed that the amount of nitrogen fertilizer was 117 kg ha-1 in 2011 and 111 kg ha-1 in 2012 in order to reach maximum yield. Doses of fertilizers above the amounts previously mentioned resulted in yield decrease. Our results indicated that in the drought year of 2012 the hybrid used available water more efficiently than in 2011. The hybrid produced 59 kg ha-1 yield in 2012 and 51.9 kg ha-1 in 2011 at an optimum nutrition level.
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Nutrient Supply Effects on the Fruit Quality of Apple (Malus domestica Borkh.)
230-235Views:316The aim of our two year study is to research the effect of nutrient supply on apple fruit quality, and to explore the relationships between selected fruit quality parameters.
Observations were made in Kálmánháza (in the eastern part of Hungary), on a commercial apple orchard. In this experiment, we studied the nutrient supply reaction of four apple cultivars (Golden Delicious, Granny Smith, Idared and Jonathan Csány) under different N and NPK doses. The following fruit quality parameters were studied: fruit diameter, fruit height, fruit weight, flesh firmness, colour-coverage and we studied the density of foliage.
The research results showed that N fertilization has a great effect on fruit quality. This is shown in the cases of increase of fruit size (fruit diameter, fruit height, fruit weight). The increase is proportional with the N doses, accordingly the highest positive difference was observed by using 100 kg/ha N doses. It is important to note that moderate N doses (75 kg/ha) plus P and K additions also had positive effects. There approached the values of 100 kg/ha N, and even exceeded its values in the cultivar Golden Reinders. The increased N doses enlarged the standard deviation, on the other hand, this parameter was low in the cases of balanced NPK fertilization. The nutrient supply increased the vegetative area (density of foliage) in addition to the generative parts, in particular only N fertilizer. However the denser foliage hindered the growth of fruit weight and colour-coverage, and also decreased the fruit quality and the flesh firmness of cultivars, which have a negative effect on storageability.
A linear correlation was demonstrated between the fruit weight and colour-coverage, or between fruit weight and flesh firmness. The character of their relationship was similar, but the direction differed: high fruit weight was with high colour-coverage, but with low flesh firmness.