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Preliminary studies on in vitro sensitivity of Venturia inaequalis populations to some fungicide active ingredients
165-168Views:127Aim of this study was to investigate the sensitivity of conidia of Spilocaea pomi to main fungicide active ingredients (cyprodinil, pirimethanil, fluquinconazole, tebuconazole, difenoconazole, dodine és trifloxistrobin) at dosages of 0.5, 1 and 2 times incubated in vitro at 24 and 48 hours collected from three integrated apple orchards. Results showed that degree of conidial germinations at the fungicide treatnemts decreased in the order of dosages of untreated, 0.5, 1 and 2 times. Considerable conidial germination was observed at higher dosages of some fungicides (e.g. groups of strobilurines and EBI) which confirmed the possibilities of sensitivity reduction of Spilocaea pomi to some funigicides in the sampled Hungarian apple orchards.
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Leaf and root evaluation of bioactive compounds of different beetroot varieties
135-139Views:220The importance of beetroot lies in the numerous health benefits, attributed to its wide-ranging array of minerals, vitamins and bioactive compounds which play a key role in the human diet. Generally, beetroot is consumed for its root, however, its leaves also contain a good amount of bioactive compounds. The experiment was carried out with four beetroot varieties in order to compare the amount of bioactive compounds in the root and the leaf. The analyzed data were total dry matter content, total polyphenol, flavonoid and vitamin C.Results have shown that roots contain the highest amount of bioactive compounds. Nevertheless, leaves can be also considered a significant source. Therefore, consumption of the young leaves is highly recommended due to their availability after thinning or at maturity stage (60 days after the germination). Of the examined varieties, ‘Libero’ demonstrated the highest total polyphenol, flavonoid and vitamin C values, although it was found to be statistically insignificant. -
Risk effects of the spread route of mycotoxins
90-95Views:162In Hungary the mycotoxin is a great problem, because there are many natural toxins in wheat and maize. These cereals can be found on
considerable proportion of the country’s sowing area, and they are deterministic food for the population. The direct human and animal
utilization of the contaminated cereals mean a serious risk in the food chain. In Hungary’s climate the soil is contaminated with pathogen
moulds, particularly Fusarium species, which increase by respective temperature and moisture content in cereals. The Fusarium can
decrease the quality of the wheat in different ways: decrease the germination capability and cause visible discoloration and appearance of
mould, reduces the dry material and nutrient content of the grain. From the toxins produced by the Fusarium genus, the trichotecene (T-2,
HT-2, deoxinivalenol, nivalenol, diacetoxyscxirpenol, Fusarenon-X) and the estrogenic zearalenon (F-2) are the most common in Hungary.
The fumonisins (FB1, FB2, FB3) first identified in 1988, relatively newly discovered, are also important. Major proportion of mycotoxins in a
healthy organization is metabolized by the enzyme system of liver and intestinal bacteria. The toxicity is reduced or even leaves off.
However, more toxic and biologically active compounds can be formed. For the reduction of mycotoxin-contamination several possibilities
are available in the case of storage, processing and feeding. -
Mitigation of environment impact of mycotoxins produced by Fusarium species
159-164Views:183The Fusarium fungi hazards the grain quality of cereals, therefore significantly affects their utilization as animal feed or consumable product. The Fusarium can decrease the quality of wheat in different ways: decreases the germination capability, causes visible discoloration, mould may appear, reduces the dry material and nutrient content of the grain, causes mycotoxin infection – as a result given by its by-product. Micotoxins produced by Fusarium genus, as the trichotecenes (T-2, HT-2, deoxynivalenol, nivalenol, diacetoxyscirpenol, Fusarenone-X) and the zearalenone (F-2) are the most common in Hungary. Occurrence of fumonisins first discovered in 1988 are must be identified carefully. About 20–30% of the overall worldwide production of cereals is infected with Fusarium and its toxins, which situation is similar in Hungary. This infection causes serious yield-losses in cereal production. In the case of cereal products, which non-utilizable as forage seems, an optimal solution is utilizing as biogas raw material, but it is also important to examine the effect of the infected cereal on the anaerobe digestion process.
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Study on the cold tolerance of maize (Zea mays L.) inbred lines in Phytotron
41-45Views:109Maize has come a long way from the tropics to the temperate zone. In the beginning, the spreading of maize was prevented by its sensitivity to cold. Improved cold tolerance at germination is one of the most important conditions for early sowing. The advantage of cold tolerant hybrids is that they can be sown earlier, allowing longer growing seasons and higher yields, due to the fact that the most sensitive period in terms of water requirements, flowering, takes place earlier, i.e. before the onset of summer drought and heat.
In Martonvásár, continuous research is carried out to improve the cold tolerance of maize. In the present experiment, the cold tolerance of 30 genetically different maize inbred lines was investigated in a Phytotron climate chamber (PGV-36). The aim of our research is to identify cold tolerant lines that can be used as parental components to produce proper cold tolerant hybrids and/or as sources of starting materials for new cold tolerant inbred lines. After observing and evaluating changes in phenological traits under cold-test, the results of the cold-tolerance traits of interest have been used to highlight several inbred lines that could be good starting materials for further research on genetic selection for cold tolerance.
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Management of phytopathogens by application of green nanobiotechnology: Emerging trends and challenges
15-22Views:325Nanotechnology is highly interdisciplinary and important research area in modern science. The use of nanomaterials offer major advantages due to their unique size, shape and significantly improved physical, chemical, biological and antimicrobial properties. Physicochemical and antimicrobial properties of metal nanoparticles have received much attention of researchers. There are different methods i.e. chemical, physical and biological for synthesis of nanoparticles. Chemical and physical methods have some limitations, and therefore, biological methods are needed to develop environment-friendly synthesis of nanoparticles. Moreover, biological method for the production of nanoparticles is simpler than chemical method as biological agents secrete large amount of enzymes, which reduce metals and can be responsible for the synthesis and capping on nanoparticles.
Biological systems for nanoparticle synthesis include plants, fungi, bacteria, yeasts, and actinomycetes. Many plant species including Opuntia ficus-indica, Azardirachta indica, Lawsonia inermis, Triticum aestivum, Hydrilla verticillata, Citrus medica, Catharanthus roseus, Avena sativa, etc., bacteria, such as Bacillus subtilis, Sulfate-Reducing Bacteria, Pseudomonas stutzeri, Lactobacillus sp., Klebsiella aerogenes, Torulopsis sp., and fungi, like Fusarium spp. Aspergillus spp., Verticillium spp., Saccharomyces cerevisae MKY3, Phoma spp. etc. have been exploited for the synthesis of different nanoparticles. Among all biological systems, fungi have been found to be more efficient system for synthesis of metal nanoparticles as they are easy to grow, produce more biomass and secret many enzymes. We proposed the term myconanotechnology (myco = fungi, nanotechnology = the creation and exploitation of materials in the size range of 1–100 nm). Myconanotechnology is the interface between mycology and nanotechnology, and is an exciting new applied interdisciplinary science that may have considerable potential, partly due to the wide range and diversity of fungi.
Nanotechnology is the promising tool to improve agricultural productivity though delivery of genes and drug molecules to target sites at cellular levels, genetic improvement, and nano-array based gene-technologies for gene expressions in plants and also use of nanoparticles-based gene transfer for breeding of varieties resistant to different pathogens and pests. The nanoparticles like copper (Cu), silver (Ag), titanium (Ti) and chitosan have shown their potential as novel antimicrobials for the management of pathogenic microorganisms affecting agricultural crops. Different experiments confirmed that fungal hyphae and conidial germination of pathogenic fungi are significantly inhibited by copper nanoparticles. The nanotechnologies can be used for the disease detection and also for its management. The progress in development of nano-herbicides, nano-fungicides and nano-pesticides will open up new avenues in the field of management of plant pathogens. The use of different nanoparticles in agriculture will increase productivity of crop. It is the necessity of time to use nanotechnology in agriculture with extensive experimental trials. However, there are challenges particularly the toxicity, which is not a big issue as compared to fungicides and pesticides.
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Definition antioxidant activity of selenium-enriched food sprout, as well as their microbiological analysis
25-30Views:255In this present study, we prepared selenium-enriched food sprouts, where the antioxidant capacity was analysed, we also determined their microbiological status. We took into account the fact, we choose micronutrients to our treatment, that selenium can be delivered to the body by a small amount with the most widely consumed food.
We focused during our research to determine that the increasing concentrations of selenium treatment, in which we used sprouts, knowing fully well that it has an impact on aboriginal antioxidant capacity of sprouts, which is mainly due to high vitamin content of sprouts.
Furthermore, we think it is important to make microbiological analysis, because germination conditions, for example temperature, pH, all this will create an ideal environment for the growth of microorganisms. So we had goal to determine, how the used selenium concentration affect the total plate count, coliform bacteria count and Staphylococcus aureus count of sprouts.
We determined the aboriginal water-soluble and lipid-soluble antioxidant capacity of sprout with the PHOTOCHEM chemiluminometer and we applied pour plate technique for the mapping of the mycrobiological state of sprouts.
Experimental results are evaluated, we state that increasing concentrations of selenite or selenate treatment, is primarily water-soluble antioxidant capacity of sprouts was affected. The water-soluble antioxidant capacity of wheat sprout was much higher than the measured values in pea sprout, this may be linked to what we measured. That is much higher ascorbic acid content in case of wheat sprout, which is well known as one of the most important antioxidant properties compounds of wheat sprout.
We conclude from the results of the microbiological, that the highest concentrations of selenite or selenate treatment has a relative significant anti-microbial effect in case of wheat sprouts. Coliform and total plate count showed no clear decreasing tendency, although the values of treatments in both cases obtained were below the control values.
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Study of plant physiological parameters in winter oilseed rape (Brassica napus var. napus f. biennis L.) production on chernozem soil
111-115Views:259We made plant physiology examinations in Arkaso winter oilseed rape hybrid substance: relative chlorophyll content (SPAD) and leaf area index (LAI) measurements. The experiment was set in University of Debrecen Agricultural Sciences Center at Látóképi Experimental Station in four replications, in two different sowing times (I. sowing date on 08/22/2014 and II. sowing date on 09/09/2014 sowing againhappened because of the incomplete germination in the second subtance 01/10/2014) Three different plant density 200, 350 and 500 thousand ha-1, under the same nutrient supply, 45 cm row spacing. The experiment was green crop of winter wheat. The relative chlorophyll content (SPAD) and leaf area index (LAI) measurements were made in seven different times. We measured the maximum value of chlorophyll content in the first sowing time at 500, and the second sowing time at 350 thousand ha-1 plant density. The measurement results proved that there was a linear relationship between the number of plants and the LAI. The maximum leaf area index values we measured in both the sowing time at 500 thousand ha-1 reached.
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Examination of the impact of sowing technology models on the ear, constiuent and yield parameters of the yield formation elements of maize hybrids of different genotypes
17-23Views:140Production year 2012 has been characterised by climatic extremities. The weather of this year can be considered very contradictory in terms of maize production. The droughty conditions of the winter and spring months had a negative effect on both germination and starting vigour. The favourable weather of May-July created ideal conditions for intensive growth and generative processes; however the lack of precipitation in August and September had a damaging effect on the development of yield composing elements and grain saturation processes as well. Under such circumstances, the sowing date models caused significant differences in the yield and quality of the hybrids belonging to different growth periods. The growing period of the maize hybrids has been shortened as a result of the unfavourable climatic conditions.
Based on the trial results, it is verifiable that short growing period hybrids can be securely sown in draughty years even with a later sowing date, however using a later sowing date in the case of longer growth period hybrids may result even in a yield loss of 2–3 t ha-1. In the case of early and average sowing dates, with given yearly conditions the hybrids of the observed FAO 370-390 hybrid group provided the best result (12.40 t ha-1, 10.99 t ha-1), while in the case of the third, late sowing date the yield dominance of the FAO 290-350 hybrid group is the most significant (10.08 t ha-1).
The analysis of the yield composing elements found that the P9578 hybrid has the highest shelling ratio, while its cob is the shortest. The P9494 hybrid has a high yield and the highest thousand grain weight, while the DKC 4983 has the longest cob and its thousand grain weight is above 300 g.
The results confirm the fact that DKC 4590 has the highest yield potential and starch content, while in terms of oil and protein content the Szegedi 386 and NK Octet hybrids are the most important.