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Cytochrome b diversity of Hungarian Botrytis cinerea strains
18-21Views:365In the mitochondrion of eukaryotes, cytochrome b is a component of respiratory chain complex III. Cytochrome b is encoded by the
cytochrome b (CYTB) gene located in the mitochondrial genome. The fungicidal activity of QoIs relies on their ability to inhibit mitochondrial respiration by binding at the so-called Qo site (the outer quinol-oxidation site) of the complex III. Since their introduction, QoIs (like azoxystrobin) have become essential components of plant disease control programs because of their wide-ranging efficacy against many agriculturally important fungal diseases like grey mould on various crops. QoI resistance primarily arises from a target-site-based mechanism involving mutations in the mitochondrial CYTB. As the management of grey mould is often dependent on chemicals, the rational design of control programs requires the information about the diversity of genes connected with resistance in field populations of the pathogen.
Monospore B. cinerea field isolates has been collected during 2008-2009 from different hosts in Hungary. PCR fragment length analysis
indicated the high frequency presence of type large intron in the isolates while in a few strains G143A substitution could also be detected.
These results indicated the heterogeneity of CYTB in the Hungarian B. cinerea populations, which possibly involve the heteroplasmy of this
mitochondrial gene, moreover indicates the existence op azoxystrobin resistant populations in Hungary.
This work was supported by NKFP-A2-2006/0017 grant. Erzsébet Fekete is a grantee of the János Bolyai Scholarship (BO/00519/09/8). -
Plant growth analysis of wheat populations in a long-term field-experiment
147-151Views:542The experiments were conducted as part of the long-term trial adjusted, in triculture (pea-wheat-corn) and biculture (wheat-corn), at three nutrition levels, with the use of one crop protection technologie (conventional) at the Látókép Research Site of the Centre of Agricultural Scienses, University of Debrecen, on a chernozem soil. The wheat variety used in the long-term trial was GK Csillag, which was sown at 5,8 million germs/ha.
The effect of pre-crops and nutrient-supply levels on some growth-parameters (LAI, HI, LAD), just as SPAD-values and yield amounts of winter wheat has been investigated in this experiment. We tried to find out the extent of relationship between the different parameters, so we determined the relationships between different nutrient-supply levels, yield amounts, LAI- SPAD- and LAD-values – measured in the crop-year of 2010–2011 in different crop rotation systems – by using correlation analysis. It has been stated both in case of bi- and tri-culture crop-rotation systems that different fertilizer dosages had significantly affected the leaf area index dynamics and its maximal value, and that increasing N+PK fertilization has
significantly increased the duration of leaves, as well. The highest SPAD-values were measured during the flowering and grain filling stages. However, we haven’t revealed significant differences between all fertilizer treatments. In case of the bi-culture crop-rotation system harvest index values showed an increasing tendency parallel to the increasing nutrient-supply levels, while in case of the tri-culture system this tendency was rather decreasing. However, these differences were
not significant. Parallel to the increasing fertilizer dosages yield results were increased in a significant extent. At the same nutrient supply-levels 2088–4615 kg ha-1 higher yields were measured in the tri-culture than in the bi-culture system. The correlation analyses have confirmed that all of the investigated parameters (yield amount, LAI, SPAD, LAD) had almost in all cases close positive correlation to the nutrient-supply level and the yield amount in both crop-rotation systems. These results have confirmed that the leaf area, the leaf duration, the SPAD-values and the fertilization have altogether resulted in the production of maximum grain yields. -
SSR based characterization of peach (Prunus persica L.) and apricot (Prunus armeniaca L.) varieties cultivated in Hungary
17-24Views:532The SSR (Simple Sequence Repeat) markers allow the discrimination of the cultivars and determination its specific DNA fingerprints. The aim of this research was to evaluate fifteen apricot (Prunus armeniaca L.) and fifty-one peach (Prunus persica L.) genotypes cultivated in Hungary to obtain their DNA fingerprints in 6 SSR (Simple Sequence Repeats) loci by allele numbers and sizes.DNAs were extracted from leaves. PCR was carried out with CY-5 fluorescent labeled Prunus microsatellite markers and the products were separated on polyacrylamide gel with ALF (Automated Laser Flourometer)-Express II.According to our results, in the case of peach genotypes, all 6 SSRs were able to amplify alleles. UDP 96 005 was the most informative marker and UCDCH 17 was the least due to its monomorphic pattern. Regarding the apricot samples BPPCT 041 did not amplify any allele. In the case of P. armeniaca UDP 96 005 had the highest heterozygosity index as well and the highest number of alleles. The least informative marker was the UCDCH 17. Since the 6 SSR were not enough to discriminate the apricot and peach genotypes, it is suggested to use more SSR primers. -
Biomass production estimation of processing tomato using AquaCrop under different irrigation treatments
131-136Views:571The wiser usage of irrigation water is inevitable in the future. Irrigation has very high input cost; therefore, farmers must carry out irrigation with care. Also, the effect of irrigation on crops has a big role in decision making. Modeling provides a possibility to evaluate this effect. AquaCrop, as a crop production simulation model has great potential in this field. The accuracy of tomato biomass yield prediction of the model was tested in this research. For collecting the necessary data, a field experiment was conducted at Szarvas on processing tomato with different water supplies, such as 100% (I100), 75% (I75), 50% (I50) of potential evapotranspiration and a control with basic water supply (C). The relation of the simulation and actual biomass yields was evaluated during the season. Very good correlation was found between the modelled and the actually harvested data. The data for the control and I100 treatments showed higher correlation than the I75 and I50. The relationship for all of the data was moderately strong. Miscalculations occur mostly when the dry biomass yield reaches
7 t ha-1. The accuracy of the model was evaluated with the use of mean absolute error (MAE) and root mean squared error (RMSE) values. The least error was found in the C treatment, which means 0.34 MAE and 0.45 t ha-1 RMSE. The simulation resulted in higher errors in the I75 and I50 treatments. -
Relation of availability and barley uptake of some potentially toxic elements
7-10Views:268A small-plot microelement load field trial was set up on brown forest clay soil with eight elements (Al, As, Cd, Cr, Cu, Hg, Pb, Zn), on 3 levels each (0/30, 90, 270 kg element ha-1). The soil was treated with soluble salts of elements once at initiation (1994). In the seventh year of the experiment (2001) winter barley was the test plant. The total element content was determined in plant samples (shoot, straw, grain) after microwave digestion using cc.HNO3+cc.H2O2. The element composition of the prepared samples was determined using ICP-MS technique. In the experiment toxic effects of treatments and yield loss could not be observed. Zn and As contents in barely shoots were only moderately increased by increasing microelement loads. Effects of Cr, Cu, Hg, Pb and Al treatments could not be observed. On the other hand, Cd accumulation was significant in the shoot. Cd content was also increased both in straw and grain. Results of this experiment prove that Cd remains mobile in the soil-plant system for a long time. Its accumulation can be observed both in vegetative and reproductive parts of plants without toxic symptoms and yield loss.
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Management of phytopathogens by application of green nanobiotechnology: Emerging trends and challenges
15-22Views:636Nanotechnology is highly interdisciplinary and important research area in modern science. The use of nanomaterials offer major advantages due to their unique size, shape and significantly improved physical, chemical, biological and antimicrobial properties. Physicochemical and antimicrobial properties of metal nanoparticles have received much attention of researchers. There are different methods i.e. chemical, physical and biological for synthesis of nanoparticles. Chemical and physical methods have some limitations, and therefore, biological methods are needed to develop environment-friendly synthesis of nanoparticles. Moreover, biological method for the production of nanoparticles is simpler than chemical method as biological agents secrete large amount of enzymes, which reduce metals and can be responsible for the synthesis and capping on nanoparticles.
Biological systems for nanoparticle synthesis include plants, fungi, bacteria, yeasts, and actinomycetes. Many plant species including Opuntia ficus-indica, Azardirachta indica, Lawsonia inermis, Triticum aestivum, Hydrilla verticillata, Citrus medica, Catharanthus roseus, Avena sativa, etc., bacteria, such as Bacillus subtilis, Sulfate-Reducing Bacteria, Pseudomonas stutzeri, Lactobacillus sp., Klebsiella aerogenes, Torulopsis sp., and fungi, like Fusarium spp. Aspergillus spp., Verticillium spp., Saccharomyces cerevisae MKY3, Phoma spp. etc. have been exploited for the synthesis of different nanoparticles. Among all biological systems, fungi have been found to be more efficient system for synthesis of metal nanoparticles as they are easy to grow, produce more biomass and secret many enzymes. We proposed the term myconanotechnology (myco = fungi, nanotechnology = the creation and exploitation of materials in the size range of 1–100 nm). Myconanotechnology is the interface between mycology and nanotechnology, and is an exciting new applied interdisciplinary science that may have considerable potential, partly due to the wide range and diversity of fungi.
Nanotechnology is the promising tool to improve agricultural productivity though delivery of genes and drug molecules to target sites at cellular levels, genetic improvement, and nano-array based gene-technologies for gene expressions in plants and also use of nanoparticles-based gene transfer for breeding of varieties resistant to different pathogens and pests. The nanoparticles like copper (Cu), silver (Ag), titanium (Ti) and chitosan have shown their potential as novel antimicrobials for the management of pathogenic microorganisms affecting agricultural crops. Different experiments confirmed that fungal hyphae and conidial germination of pathogenic fungi are significantly inhibited by copper nanoparticles. The nanotechnologies can be used for the disease detection and also for its management. The progress in development of nano-herbicides, nano-fungicides and nano-pesticides will open up new avenues in the field of management of plant pathogens. The use of different nanoparticles in agriculture will increase productivity of crop. It is the necessity of time to use nanotechnology in agriculture with extensive experimental trials. However, there are challenges particularly the toxicity, which is not a big issue as compared to fungicides and pesticides.
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Influence of temperature and variety on seed germination of soybean (Glycine max L. Merr) at different germination times
5-12Views:586Seed germination is an important stage in crop development that affects plant performance, crop yield, and quality. Many factors influence seed germination, and one of the most important factors is temperature. The closer the temperature is to the optimum, the faster germination will occur. Temperature affects seed germination in various plants and varies depending on the variety. Therefore, the effect of temperature on seed germination is necessary to investigate, also for soybean varieties. Soybean is one of the world's most valuable oil-seed crops. Generally, proper seedling establishment and germination of soybean seeds are crucial processes in the survival and growth cycle of the crop. Thus, a study was done to investigate the influence of temperature and variety on soybean seed germination at different times after sowing. The experiment was carried out at the Institute of Agronomy, Hungarian University of Agriculture and Life Sciences (MATE), Crop Production Laboratory. Seeds of two soybean varieties were subjected to three different temperatures: 15, 25, and 35 °C, and two soybean varieties, Martina and Johanna were tested. There were four germination times based on the days after sowing: Days 3, 5, 7, and 9. This experiment was set up with a completely randomized block design and four replications. According to the findings of this study, the Martina variety showed a better germination rate as well as higher vigor and viability than the Johanna variety in the first 12 days after sowing. At temperatures of 15 °C and 25 °C, both varieties achieved comparable percentages of viability, but they were more vigorous at 25 °C due to better total seedling length. Thus, the information gained from this study will indirectly determine and confirm the proper temperature for the initial growth of the Martina and Johanna varieties.
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Búzaminták fungicid szermaradvány ellenőrzése és gombafertőzöttségének kimutatása
45-51Views:213To protect crops with chemicals against different microorganisms are very important because residues of toxins could pollute infected grains. This could have the meaning of serious danger to the human and animal health. Similarly important is knowledge of details about presence or absence of any practically applied protective compounds. Experimental studies were performed on fungicide treated cereals of the Cereal Research Non-Profit Company, Szeged. Chromatographic methods were used (GC and some GC-MS) to find low level fungicide residues, microwave-assisted isolation of ergosterol was followed by HPLC to detect possible fungal infections and a more detailed microbiological analysis completed the work. Concerning the dry year 2000, no fungicides were found in the samples (by November), the ergosterol level was found to be less than 8 ppm, meaning good quality, coupled with an extremely low level of fungal infections. This was confirmed by direct microbiological testing. In comparison with the microbiological results from 1999, a negative difference can be recognized, most probably due to the rainy weather throughout the year.
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Impact possibility of hedgerows on plant protection and on the yield of winter wheat
95-101Views:328Agricultural hedgerows have various impacts on the surrounding cultivated area and on the landscape. They serve as a windbreak, help manage humidity and precipitation, and are also sources of plant species richness which can contribute to the animal biodiversity. One of their beneficial effects is the yield improvement of agricultural crops. Our investigation tries to prove this hypothesis in Hungarian circumstances in the case of several winter wheat fields near Debrecen. Regarding the results, it seems that the majority of data supports the relevant, mainly German and Austrian information, but in some cases, the correlation has not been significant.
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Practical experiences of a designing and operating a pilot aquaponic system
27-32Views:900Aquaponics is the combination of fish farming (aquaculture) and the soilless cultivation of plants (hydroponics). The aquaponics system is an artificial, recirculating ecosystem, in which bacterial processes convert the waste materials in the water used for fish rearing into plant nutrients, and therefore with the generated heat it is suitable for culturing economically valuable plants, and thus it mitigates the nutrient laden and quantity of the intensive fish producing systems’ effluent water.
The primary goal of our 12 separate unit’s aquaponics system was to gain experience. We would like to find the right plant species, which are fit for that medium, and their crop can be sold. Besides the plants, our attention focused on the fish. Two fish species were included in the experiments, the common carp and barramundi. It was difficult to create them a perfect living space, besides a constantly changing conditions temperature. Apart the above mentioned we had a problem with the number of individuals per tank, the deformity of the fish body and the too high volume of pH (we registered continuous values above 8.4). We get by carps 4.7 grams of weight gain during 15 weeks, because of the bad conditions.
The main problems at the plants are caused by aphesis and protection against sunburn. Even so we have got the multiples of field yields for each plant species. At salad has grown twice of field yields, tomatoes one and half, kohlrabi than 3.5 times more. The causes of multiple yields are the continuous balanced water and the nutrient uptake of plants. Each plant species fit for cultivating in aquaponics and their crops are delicious, chemical -free, safe and marketable. The plants should be more concentrated. After the experiment, it has been determinated that the carp is suitable for aquaponics, but greater weight gain could be achieved with optimal selection of size of rearing units.
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The effects of drought stress on soybean (Glycine max (L.) Merr.) growth, physiology and quality – Review
19-24Views:526Abiotic stresses are one of the most limiting factors inhibit plant's growth, leading to a serious production loss. Drought stress is one of the most destructive abiotic stresses and is still increasing year after year resulting in serious yield losses in many regions of the world,
consequently, affecting world’s food security for the increasing world population. Soybean is an important grain legume. It is one of the five major crops in the world, an essential source of oil, protein, macronutrients and minerals, and it is known as the main source of plant oil and protein. Harvested area of soybean is increasing globally year after year. However, soybean is the highest drought stress sensitive crop, the water deficit influences the physiology, production and seed composition of this crop. We introduce a review for literatures concerning the changes of the above traits of soybean exposed to drought stress, with past explanations for these changes. -
Establishing regional cultivating districts on the basis of the Kreybig practical soil mapping system
20-25Views:572With the help of this report evaluating the current situation of the region, characteristics of the development in agricultural production and regional differences can be clarified. By mapping out the regional soil, land use and climatic conditions and organizing these into a geographical information system, one can easily determine which plants are the most ideal to cultivate in that particular region. Moreover, it is a useful tool that enables us to
establish the most favorable land use structure suited to ecological demands and also helps to determine the methods of soil protection.
During our work, we chose administrative units in Szabolcs-Szatmár-Bereg County, based on the latest aspects of regional cultivation.
Our pilot areas are: the small regions of Nyíregyháza, Nyírbátor, Nagykálló, Mátészalka and Csenger.
Using the database, we separated and uncovered the soil conditions of the pilot areas: the chemical and physical properties of the soil layer which is exploited by the roots of the plants, the humus content, the nutrient supply, the thickness of the cultivated layer and the water management conditions.
We separated the districts of regional cultivation, where the basic elements of the traditional Kreybig color systems were applied (light yellow, dark yellow, light brown, dark green, blue, pink, red, gray, greenish brown, reddish purple, light purple, dark purple, light green).
By using the data collected from the pilot areas, we compiled a map database, which is suitable to illustrate the plant cultivating characteristics of the region. We made recommendations to determine the most favorable plants to cultivate in the specific region with the given meteorological and soil conditions, as well as for the shifting of crops.
Our recommendations were also illustrated in a map with a resolution of 1:25000. -
Influences of water deficiency on the productivity of young plants at different sites
371-378Views:309Water deficiency has become one of the most limiting factors of crop production in Hungary as the tendency in annual amounts of precipitation shows a decreasing tendency; therefore, it has become similar to those of Southern Europe. The most significant decrease in precipitation occurs typically during spring, approximately 20% of the data expressed in the averages of the last century. Studying the relationship between water deficiency as a stress factor and nutrient supply is important in order to improve the production efficiency of crops. Nowadays, this problem receives outstanding attention presented in numerous papers both in Hungary and globally, however, there are several questions yet to be answered. Our pot experiments were carried out under controlled greenhouse conditions in order to establish new data on these relationships. Experimental soils were typical for Western Transdanubia, taken from long-term field experiments representing four different site characteristics of the region. It was concluded from the results that drought periods during the early growth stages (i.e. 4–5 weeks after emergence) of plants may result in significant decreases in both dry matter production, nutrient concentrations, nutrient uptake and shoot:root ratios. Better nutrient supply, especially potassium, plays a significant role in reducing the negative effects of water deficiency.
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Water relations composition among Egyptian cotton genotypes under water deficit
5-15Views:468Background: water shortage is one of the major factor effects on growth characters and yield of most crops. Objective: this study was conducted to get to know the reactions of some Egyptian cotton genotypes to water deficit. Methods: The genetic materials used in this study included thirteen cotton genotypes belonging to Gossypium barbadense L., from the Cotton Research Institute (CRI), which was devoted to establishing the experimental materials for this investigation. Results: the ratio of GCA/SCA was less than unity for all studied indices, indicating predominance of non-additive gene action (dominance and epistasis), which is an important in exploitation of heterosis through hybrid breeding. Results: The data showed significant reduction in water relationship characters for all parental genotypes under stress conditions. The Egyptian variety Giza 68 gave high values for most water relationship characters. Data revealed that the greater the value of tolerance index is, the larger the yield reduction is under water deficit conditions and the higher the stress sensitivity is becoming. The parental genotypes Giza 96 showed the highest reduction in yield under water deficit conditions. At the same time, the cross combination Minufy x Australy showed higher values of yield reduction followed by the combinations Giza 67 x Australy. Of the male parents, the Russian genotype 10229 recorded the best GCA values for most water relationship characters. At the same time, the female parents, the old Egyptian genotype Giza 67 recorded the best values and exhibited good general combined for most water relationship characters. The cross combinations Giza 86 x Pima S6, Giza 77 x Pima S6, Giza 94 x Dandra and Giza 96 x Australy showed significant desirable SCA effect for most characters. Conclusion: relative water content %, osmotic pressure, chlorophyll and carotenoids content indicates better availability of water in the cell, which increases the photosynthetic rate. Also, the higher level of proline accumulation in the leaves which was recorded under deficit water suggests that the production of proline is probably a common response of plant under water deficit conditions.
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Regulation in Hungary of the Use of Waste Water and Sewage Sludge in Agriculture
143-149Views:353Regulating the use of waste water and sewage sludge in agriculture in such a way as to prevent harmful effects on soil, vegetation, animals and man.
In European Union there is a Council Directive (86/278/EEC) on the protection of the environment, and in particular of the soil, when sewage sludge is used in agriculture.
In the enlargement process of the European Union the Hungarian Government created a new rule (50/2001. (IV. 3.) Government regulation) which regulate using of waste water and sewage sludge in agriculture. This Hungarian rule is legal and reconcilable with the Council Directive.
The Regulation lays down limit values for concentrations of heavy metals in the soil, in waste water, in sludge and for the maximum annual quantities of heavy metals which may be introduced into the soil.
Waste water, sludge and soil on which it is used must be sampled and analysed.
Sewage sludge must be treated for six months before being used in agriculture.
The use of waste water and sludge prohibited on grassland, on nature reserved areas, in ecological farming, and soil in witch fruit and vegetable crops are growing, with the exception of fruit trees.
The states soil conservation authority must keep records registering the following:
– the quantities of waste water and sludge produced;
– the composition and properties of sludge;
– the type of treatment carried out;
– the names and addresses of the recipients of the sludge and places where the sludge is to be used.
The Government every four years must prepare a consolidated report on the use of sludge in agriculture, specifying quantities used, criteria followed and any difficulties encountered. This report must be forwarded to the Commission.
Last but not least in the light of Member States reports, the Commission will if necessary submit appropriate proposals for increased protection of the soil and the environment. -
A dual infection of two microscopic fungi on common milkweed (Asclepias syriaca) in Hajdúság region (East-Hungary)
189-195Views:384Common milkweed (Asclepias syriaca L.) is one of the most noxious and invasive weed species in Hungary. A. syriaca invades arable lands, horticultural and forestry plantations, natural and semi-natural habitats too. In cases of field crops it can cause considerable yield losses mostly in maize (2–10%), soybean (12–32%) and sorghum (4–29%), but only with high rate of coverage. It can also increase these problems that the common milkweed can be serve as reservoir and host for viruses, other pathogens and pests.
Because of the importance of common milkweed and in spite of demand to develop effective biological control, until now has not been developed a proper control program against A. syriaca. The aim of our research was to identify the necrotrophic fungal pathogens, which were involved in notable disease occurrence on this weed in different parts of Hajdúság region of Eastern-Hungary in 2016.
To the isolation of fungi from leaves and their identification were based on morphological colony characters on potato dextrose agar (PDA) and Sabouraud dextrose agar (SDA). To the description of conidia features were used PDA for Alternaria and synthetic low-nutrient agar (SNA) for Fusarium species, respectively. The examination of axenic cultures revealed that the fungi isolated from the leaves of common milkweed were Fusarium sporotrichioides and Alternaria alternata. -
Effect of mineral fertilizers with different sulphur content on the yield of winter wheat in a small plot experiment
97-100Views:675We can find more and more references on the importance of sulphur and sulphur fertilization, mainly in the case of most important field crops, as cereals, maize, and the oilseeds. The traditional sulphur sources continuously lost their importance by the stringent air pollution orders and nowadays they are not able to meet the demands of plants. Besides, the application of superphosphate, as phosphorus mineral fertilizer, decreased in several countries. Sulphur fertilization is required in most cases to reach the required yields and quality parameters. The lack of
sulphur causes decreased nitrogen utilization and yield by cereals as winter wheat, moreover, results less favourable baking parameters.
We have examined the N and S content of different parts of winter wheat plants in a small plot experiment at the University of Debrecen, Centre of Agricultural Sciences, Látókép Experimental Station. We have also studied the effect of different sulphur fertilization forms and doses on the baking quality parameters and yield. Our aim was to contribute to the development of yield and quality improving sulphur fertilization methods, adaptable by practice.
We have measured the lowest yield in the case of one of the control plots, and 100 kg/ha nitrogen, 80 kg/ha potassium and 70 kg/ha phosphorus nutrient supply resulted the lowest yield (8.7 kg/plot). The maximum yield, 10.2 kg/plot was also experienced on superphosphate fertilized plot by the application of 140 kg/ha phosphorus dose. Application the first dose of both Biofert and FitoHorm 32 S leaf fertilizers raised the yield from
9.1 to 9.9 kg/plot. ANOVA proved that Biofert and FitoHorm 32 S had no significant effect on the amount of yield, the increase is only a tendency. -
The impact of grain maize and sunflower as previous crops on the yield and ear number of winter wheat in a long-term experiment
75-79Views:297We have analyzed the effect of grain maize and sunflower forecrop on three different winter wheat genotype (GK Csillag, Mv Csárdás, Mv Toldi) regarding to the spike number and yield in different fertilization levels in the 2012/2013 cropyear. According to our data after sunflower forecrop we reached higher spike number and yield, which were influenced by the differences of the varieties. We found significant differences between the varieties in the control treatment. According to our research, GK Csillag has reached the highest yield in the examined fertilization levels and forecrop models too.