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Development of seed analyses by means of various matrix solutions and the MALDI-TOF MS technique
53-57Views:935The earth's population is growing steadily, currently accounting for about 7.3 billion people. Population growth causes food demand to rise, approximately 36 million people die each year due to starvation or related diseases. One solution to this problem is the continuous examination and development of the agricultural economy. In this study, matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI -TOF MS) were used to analyse of sunflower, soybean and hemp. In order to analyse the protein of maize, this method has already been applied. However, for sunflower, soy and hemp, it is necessary to develop a sample preparation method. Choosing the optimal matrix solution for ionization the traget molecule is an essential part of developing the method. Our aim is to compare two different matrix solutions (α-HCCA, SA matrix), based on the properties (intensity, noise ratio, value of spectra) of the spectra.
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Flesh firmness examination of scab-resistant apple varieties in a storage experiment
93-98Views:363Of the different physical characteristics of fruits, one of the most essential qualities is flesh firmness, as is an important parameter when selling fresh apples, processing the fruit (processing industry) and during its storage. In the scope of our research, we examined five apple varieties originating from the Derecske horticultural site of KITE cPlc. in 2016. The plantation was planted to be suitable for intensive apple production. Samples were collected at the time of maturity (August-October) of the given variety. The five examined apple varieties (Gaia, Isaaq, Modí, Smeralda and Fujion) are all resistant to apple scab (fungal disease caused by Venturia inaequalis). The two-month storage experiment was conducted at 16-17 °C, which had an aggressive effect on our stored apples. Flesh firmness, weight and diameter were measured on three dates for each of the five apple varieties. Our results were evaluated using IBM SPSS Statistics 20 software. We aimed to compare flesh firmness, weight and diameter of the five apple varieties and their changes during storage. Isaaq (86.34 N/cm2), Modí (94.06 N/cm2) and Fujion (84.90 N/cm2) had outstanding initial flesh firmness results. The Modí apple variety provided the best storage parameters.
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Development of the antioxidant indexes (FRAP, TFC, TPC) of scabbing resistant apple varieties in storage
89-94Views:321One of the most important groups of bioactive substances in apples are antioxidants, which have a health-preserving effect. The amount of these compounds varies not only during the ripening of the apple fruit, but also continuously during post-harvest storage. A growing group of health-conscious consumers are looking for apple varieties with a nutritional value that satisfies their needs.
In the scope of our research, we examined 5 apple varieties originating from the Derecske horticultural site of KITE cPlc. in 2016. The plantation was planted to be suitable for intensive apple production. Samples were collected at the time of maturity (August-October) of the given variety. The 5 examined apple varieties (Gaia, Isaaq, Modí, Smeralda and Fujion) are all resistant to apple scab (fungal disease caused by Venturia inaequalis). The 2-month storage experiment was conducted at 16–17 °C, which had an aggressive effect on our stored apples. Total polyphenol (TPC) and flavonoid (TFC) content as well as FRAP values of the apples were measured. Measurements were taken immediately after harvest and after 1 and 2 months of storage. Our results were evaluated by using the IBM SPSS Statistics 20 software. Our aim was to compare the antioxidant results of 5 scab-resistant apple varieties, which change continuously during storage.
The antioxidant content of our apple varieties increased during 2 months storage. The best result was achieved by the Modí apple variety (FRAP: 467.32 mg/100g; TFC: 317.76 mg/100g; TPC: 1771.97 mg/100g). Consumers may want to consume apples stored for a longer period of time if they are to absorb large amounts of antioxidants.
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Effect of sowing technology on the yield and harvest grain moisture content of maize (Zea mays L.) hybrids with different genotypes
17-22Views:322From the aspect of the efficiency of maize production harvest grain moisture content shall be considered beside the amount of harvested grain yield. Hybrids with different genotypes and vegetation period length lose their moisture content different that is affected by row spacing and plant density – among agrotechnical production factors – depending on the given crop year. In the present research work three crop years with different weather conditions were studied (2013, 2014, and 2015). The small-plot field experiment was set up at the Látókép Field Research Centre of the University of Debrecen, Centre for Agricultural Sciences with four replications on a chernozem soil type. The effect of three factors was analysed in the experiment on yield amount and its moisture content. Factors were row spacing (45 and 76 cm), plant density (50, 70 and 90 thousand plants ha-1), while hybrids were of very early (Sarolta: FAO 290), early (DKC 4014: FAO 320, P 9175: FAO 330, P 9494: FAO 390) and medium (SY Afinity: FAO 470) ripening.
In the crop year of 2013 the highest yield was produced – regarding the average of the hybrids – by the application of a row spacing of 45 cm (4.5%, 673 kg ha-1), however there was no significant difference between the yield of the populations of different row spacings. Significant difference (14.9%, 1751 kg ha-1; 6.3%, 583 kg ha-1) could be found in case of yield between different row spacing applications in 2014 and 2015. The effect of insufficiently distributed low amount of precipitation and lasting heat days in 2015 could be revealed in yield amounts and harvest grain yield moisture content results that were lower than in the previous years. In 2015 grain yield moisture content varied between 10.3 and 13.9% in case of a row spacing of 45 cm, while by 76 cm between 11.0 and 13.9%.
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Effect of different sources and doses of sulphur on yield, nutrient content and uptake by spring wheat
109-115Views:499The objective of this study was to investigate the effect of two sulphur forms (sulphate and tiosulphate) in combination with three different N:S ratios on the yield of spring wheat and total N- and S-content and uptake by the aboveground biomass on chernozem and sandy soil. In the greenhouse experiment, the effects of two sulphur forms were compared: sulphate (SO42-) and thiosulphate (S2O32-). The sulphate was applied as potassium-sulphate (K2SO4) and thiosulphate as ammonium-thiosulphate ((NH4)2S2O3). Increasing doses of both sulphur forms (24, 60, 120 kg S ha-1) were used with the same nitrogen dose (120 kg N ha-1) which caused three different N:S ratios background (1:0.2, 1:0.5, 1:1). Nitrogen was supplied in the form of monoammonium-phosphate (MAP), ammonium-nitrate and ammonium-thiosulphate. Plant samples were taken in three different development stages of spring wheat based on the BBCH scale: at the stage of BBCH 30–32 (stem elongation), BBCH 65–69 (flowering) and BBCH 89 (ripening). The total nitrogen and total sulphur content of plant at different development stages and also wheat grain were measured by Elementar Vario EL type CNS analyser. The nutrient uptake by plant and grain was calculated from the yield of spring wheat and the N and S content of plant. The grain yield on chernozem soil ranged between 6.31 and 12.13 g/pot. All fertilised treatments significantly increased the grain yield compared to the control. The highest yield was obtained in the case of the application of 120 kg N ha-1 and 60 kg S ha-1in sulphate form. The grain yield on sandy soil varied from 2.53 to 6.62 g/pot. The fertilised treatments significantly enhanced the yield compared to the control. The highest yield was observed in the case of the application of 120 kg N ha-1 and 60 kg S ha-1 in thiosulphate form. On chernozem soil the increasing doses of sulphur (24, 60, 120 kg S ha-1) with the same N dose (120 kg N ha-1) increased the N-content of spring wheat at all development stages and in the grain. The treatments with different sulphur sources did not cause further changes in the N-content. On sandy soil in the most cases the N-content did not change significantly as a result of increasing sulphur doses. The treatments with sulphate form basically resulted higher nitrogen-content than treatments with thiosulphate form. The treatments with increasing sulphur doses resulted higher S-content on both of chernozem and sandy soil in the case of all development stage. Comparing the effect of the applied sulphur sources on the S-content it can be stated that at the stage of BBCH 30–31 and 65–69 the treatments with sulphate form resulted higher sulphur-content. At the stage of BBCH 89 there was no significant differences in S-content of grain as a result of different sulphur-sources.
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The synthesis of selenium nаnоpаrtiсle (SeNPs) – Review
5-8Views:605Selenium is an important dietary miсrоnutriеnt required for the nоrmаl physiоlоgy and mеtаbоlism of humans and аnimаls. The biоlоgicаl prоperties оf selenium nаnо pаrticle depend оn their size аnd fоrms. Sеlenium nаnоpаrticle (SeNPs) аttrасts еvеn mоrе аttеntiоn, thanks to its high biоаvаilаbility аnd muсh lоwеr tохiсity thаn inоrgаniс аnd оrgаniс fоrms. In this review, I summаrized the infоrmаtiоn аnd wаys оf prоductiоn оf selenium nаnоpаrticle. SeNPs hаve been prоduced in chemicаl, physicаl, аnd biоlоgicаl wаys. In recent years, biоlоgicаl wаys hаve been especially important in the prоductiоn оf selenium nanoparticles.
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Biostimulant induce growth, chlorophyll content and fresh herbage yield of alfalfa (Medicago sativa L.) and variegated alfalfa (Medicago × varia Martyn) plant
19-25Views:511The use of biostimulants is associated with promoting plant growth by stimulating cell division and improving nutrient availability and uptake. A study was conducted at the University of Debrecen, Hungary, to examine the effect of biostimulants on alfalfa growth, chlorophyll content and fresh herbage yield. The experiment was arranged in a randomised complete block design with three biostimulant treatments plus control replicated three times. Data collected were subjected to analyses of variance using Genstat, where significantly different means were separated at a probability of 5% using the least significant difference. The findings show no different variation in plant height or chlorophyll content (SPAD) throughout the early stages of growth. Nonetheless, a notable impact was noted in the latter stages (28 days after biostimulant treatment application) on the growth of the alfalfa plant. Biostimulant treatments did not had effect on fresh yield for second through fourth cuts, but the fifth cut showed a significant effect, with T1 treatment recording the highest herbage yield of 19745 kg ha-1 followed by T2 (Tricho Immun plus Ino Green) and T3 (Tricho Immun), with yields of 19528 kg ha-1 and 17273 kg ha-1, respectively, while the T0 (control) recorded the lowest herbage yield of 12060 kg ha-1. However, the average mean yield indicated the application of biostimulants significantly increased fresh yield herbage by 20.5%. Correlation coefficient values suggested plant height at both 14 and 28 DAH (days after harvest) strongly correlated with fresh herbage yield (r = 0.7756 and 0.7455) which reflected in the increase in fresh herbage yield. Therefore, our results suggest that the use of biostimulants in alfalfa cultivation holds promise for improving growth and yield potential through their positive effects on chlorophyll content and the growth of alfalfa plant.