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The effect of diet composition, a probiotic and a symbiotic treatment on the ileal microbiota composition of one-week-old broiler chickens
213-220Views:293A healthy microbiota present in the small intestine contributes significantly to small intestinal function, including digestion, nutrient absorption and health. The current study investigated the effects of a prebiotic, a probiotic and a symbiotic supplementation on ileal microbiota composition of broilers at 7 days of age. In a total of 574 male Ross 308 day-old chickens were divided into four groups using six replicate pens and 24 chickens per pen. A maise-soybean based control diet (C), a control diet supplemented with probiotics (Broilact; Br), a control diet supplemented with symbiotic (inulin, yeast, Bacillus subtilis; Sy) and a wheat based diet supplemented with wheat bran (W) were formulated. On day 7 of life, two chickens per pen were slaughtered and ileal chymus samples were collected. For microbiota analysis 16S rRNA (V3-V4 region) gene targeted Illumina MiSeq sequencing was used. Feeding all diets supplemented increased the diversity to varying degrees compared to the control (C) diet (p=0.006). As a conclusion, all supplementation substantially influenced ileal microbiota of broiler chickens at an early age. All these results could offer some information for the future study on the relationship between early intestinal microbiota and the compounds of the feed.
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The effect of sous-vide cooking on the antioxidant properties of oyster mushroom (Pleurotus ostreatus L.)
177-184Views:513Oyster mushrooms (Pleurotus ostreatus L.) are renowned for their antioxidant, antimicrobial, and prebiotic properties. This study explores the antioxidant characteristics, activity, and β-glucan content in freeze-dried mushroom samples, investigating the influence of sous-vide cooking. Uncooked freeze-dried P. ostreatus and three pre-cooked freeze-dried samples (70, 80, 90 °C through 4 hours) were analysed for Total Polyphenol Content (TPC), Total Flavonoid Content (TFC), Radical Scavenging (DPPH), Ferric Reducing Antioxidant Power (FRAP), and β-glucans content via HPLC and Total Dietary Fiber (TDF) via enzymatic gravimetric method. Results indicate that uncooked mushroom powder exhibited superior antioxidant capabilities compared to cooked samples. The sous-vide cooked (80 °C) mushrooms displayed the highest total phenolic and flavonoid content. Moreover, pre-cooked (70 °C) mushroom powder demonstrated the highest β-glucan content, significantly surpassing the uncooked control sample. Notably, pre-cooked groups (80, 90 °C) demonstrated significantly higher TDF levels compared to uncooked sample. This research offers valuable insights into the potential use of mushrooms as high-antioxidant, antimicrobial, and prebiotic food or feed supplements, with broad implications across various fields.
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Evaluation of the synergistic effect of yeast and Chicory-inulin on rumen fermentation parameters and estimation of methane emission
13-18Views:221Probiotics and prebiotics are feed additives that have been extensively utilised in animal nutrition for a considerable duration. However, only some studies have investigated their synergistic effects on rumen fermentation parameters and their role in minimizing methane emission. Therefore, this study examined the impact of combining chicory-inulin with yeast (Saccharomyces cerevisiae) on rumen fermentation parameters and methane emissions in weaned dairy goats. The feeds were formulated into eight diets and offered to 24 Saanen×Toggenburg crossbred weaned female dairy goats weighing 14±0.5 Kg in a Completely Randomized Design with a (4x2) factorial arrangement. The diets were Rhodes grass hay and chicory supplementation at four levels: 0, 10, 20, 20, and 30% as the main effects and with (+) and without yeast (-) yeast as interaction levels. The findings indicated that the inclusion of yeast and Chicory had a significant effect (p<0.05) on rumen pH, ammonia nitrogen concentrations, production of volatile fatty acids), and estimated methane emission. The highest pH was recorded in T1- with 7.27. It was followed closely by T1+ with 6.73. T1+ recorded the highest methane (39.67 mmol/L), while T4- had the lowest (32.42 mmol/L). This study concludes that Chicory-inulin has prebiotic properties by maintaining pH levels and affecting amounts of ammonia nitrogen. The lack of a significant interaction effect between yeast and Chicory in methane emission implies that their combined influence may not significantly affect methane emissions in the current study's experimental settings.