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Detection of DNA mutations by PCR-TTGE method
21-25Views:619In our study PCR-temporal temperature gelelectrophoresis (TTGE) and MeltINGENY bioinformatic program were used to analyse the mutations in the genes of melanocortin-1 receptor (MC1R) and pituitary adenylate-cyclase activating polypeptide (PACAP) in cattle. Amplification of target DNA by PCR was performed with GC-clamp primers and non-GC-clamp primers in simplex PCR reactions. The fragments were separated by denaturing polyacrylamide gelelectrophoresis (denaturing agents: high temperature, urea) after PCR reactions.MC1R homozygous individuals were used for the reaction.
We concluded that MeltINGENY program makes the decision and detection system easier, and more simple as the melting profile of target sequence is determined by the software. In case of MC1R gene, PCR-TTGE method is appropriate for SNP detection, however PACAP gene polymorphism can not be identified by the method, because PACAP mutations are not included in melting domains, therefore PCR-TTGE cannot detect them.
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Genetic and phenotypic basis of goat adaptability across agro-ecological zones: Implications for breeding and conservation
51-58Views:104Goats are among the most adaptable livestock species that can survive in varied agro-ecological zones globally. This resilience is shaped by the interactions between genetic and phenotypic traits. This review assesses the available information on morphology, physiology, and molecular characteristics that enable them to adapt and their implication for breeding and conservation. Phenotypic characteristics, including variation in coat color, the type and density of hair, body size, skin color, and thermoregulation behavior, were observed to be measures of adaptation to heat, cold, and feed scarcity. The review also observed some key candidate genes at the molecular level, including HSP70, EPAS1, FGF5, and MC1R, among others, with pathways that are responsible for heat tolerance, hypoxia response, and metabolic efficiency. The link between environmental pressures and phenotypic variation is examined as a driver for genetic differentiation among local goat populations. Incorporating these phenotypic and genetic insights forms a basis for breeding strategies that are climate-resilient and for safeguarding adaptive genetic resources. This will ensure that goats stay productive and diverse over time, thereby contributing to food security and the current climate change.