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Unraveling changes in the duck microbiome and inflammatory processes due to allithiamine-enriched feed
163-171Views:561The gastrointestinal tract of poultry harbors a diverse and intricate microbiome that plays a crucial role in nutrient digestion and absorption, immune system development, and enhances resistance against pathogens. Maintaining a healthy state and proper production is fundamentally determined by the symbiosis between the host and microbes. Due to genetic and technological improvements, intensive growth rate can be associated with many pathological conditions, such as increased susceptibility to infections. Intestinal inflammation in poultry industries detrimentally affects productivity by hindering nutrient absorption and the efficient allocation of nutrients for growth. The host releases different biomarkers in response to inflammation. Hence, there is an utmost interest of reliable, precise, sensitive and robust biomarkers to evaluate both the gastrointestinal health status and inflammation in poultry. The aim of this study was to determine how the developed feed prototype (allithiamine) affects the community diversity in raised duck, and the relationship between gut microbiome composition and inflammatory factor as calprotectin, using targeted 16S rRNA gene amplicon sequencing and Chicken Calprotectin ELISA Kit.
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Investigation of the effect of allithiamine-enriched feed on the poultry gut microbiome composition and resistome
149-155Views:272Over the past 20–25 years, the poultry industry has evolved into a specific protein production system. However, the stress resulting from intensive rearing practices has led to numerous negative consequences, making the optimisation of livestock gut microbiome composition crucial for mitigating these effects. Advancements in modern molecular biology methods have brought attention to the impacts of nutrients on gut microbiota. In our study, we extensively investigated the changes induced by feed formulations rich in phytonutrients on the gastrointestinal microbiota of livestock using targeted 16S rRNA amplicon sequencing. Our objective is to examine how the developed feed prototype affects the composition of core microbiomes in raised poultry, community diversity, and the resilience of complex microbial networks. We seek correlations between biological livestock and environmental samples to identify which community constituents, in what proportions and occurrences, may play a role in the development of specific diseases. Based on our measurement results, it can be asserted that allithiamine positively modulated "beneficial" community constituents. Beyond the impact of allithiamine-enriched feed rich in phytonutrients, the composition of the microbial community in the poultry gastrointestinal tract is significantly influenced by the age of the birds. Furthermore, due to the presence of multi-drug-resistant pathogens in environmental samples from livestock facilities, appropriate transmission risk management measures are of paramount importance.
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Inheritance of Plant and Ear Height in Maize (Zea Mays L.)
34-38Views:674Plant and ear height are very important characters not only for describing new varieties of maize (Zea mays L.), but for green and dry matter production, and even for grain yield. Significant positive correlations have been reported by various authors between plant height and stover yield, plant height and dry matter yield, and plant height and grain yield. The height of the main ear is also correlated to plant height. It depends on the variety or the environment, but is likely to be the same height within a population. Many environmental and agronomical factors (e.g. plant density, fertilization, pests and diseases) influence the expression of these characters, which are not quality traits. Their expression is controlled by many genes and by the interactions between these genes. The heritability of these traits is high and they show significant genotypic variability and positive heterosis, as reported in many research publications.
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Studies on the Fusarium stalk rot infection of the maize genotypes using the Findex percentage and a computerised image analysis program
45-51Views:247In a continental climate, the pathogens causing the most serious problems are species belonging to the Fusarium genus. When the pathogen attacks the stalk, the plant dies earlier, reducing grain filling and resulting in small, light ears. In addition, the stalks break or lodge, resulting in further yield losses from ears that cannot be harvested. During the three years of the experiment, 14 inbred lines were examined. The genotypes were sown in a two-factor split-plot design with four replications, with the genotypes in the main plots and four treatments in the subplots: two Fusarium graminearum isolates (1. FG36, 2. FGH4), 3. sterile kernels, 4. untreated control. The results experiments showed significant differences between the genotypes for resistance to fusarium stalk rot. Among the inbred lines the best resistance to fusarium stalk rot was exhibited by P06 and P07, both of which were related to ISSS. The precision and sensitivity of disease evaluations carried out visually and using image analysis software were compared in the experiment, and with two exceptions the CV values were lower for the image analysis. As the CV for measurements can be considered as a relative error, it can be stated that image analysis is the more precise of the two methods, so this technique gives a more accurate picture of the extent of stalk rot. The extent of stalk rot developing in response to natural infection is extremely environment-dependent, so the use of artificial inoculation is recommended for selection trials.
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Role of Sowing Time in Maize Production (Review)
36-39Views:203Many authors, both in Hungary and abroad, have reported on experiments carried out to determine the role of sowing time in maize, but the results are often contradictory. This is hardly surprising, since the maize plant exhibits enormous genetic variability and the hybrids created through selection and inbreeding may have very specific requirements as to sowing date. The year effect, too, often complicates the efforts of scientists to provide clear guidance to farmers on the optimal sowing date for each hybrid.