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Application of the Collision Cell (CCT) in the ICP-MS Analytical System
120-125Views:164Our laboratory has seen a sharp rise in the number of requests for the analysis of smaller and smaller concentrations of elements from foods, plants, soil, organic fertiliser, irrigation and ground water, sewage, sewage sludge, raw material of food, as well as human and animal origin samples. From the above elements the inductively coupled plasma mass spectrometer is capable of analysing the smallest concentration of elements. Our method of analysis is to use our ICP-MS instrument, together with a ICP-MS with Collision Cell Technology (CCT). The CCT method has better detection limit, with 1-3 magnitudes, compared to the normal ICP-MS analytical method. The CCT has better detection limits mainly for the following elements in the periodical system: analysis of arsenic, selenium, germanium, vanadium and chromium. Additionally a collision cell can be applied for the analysis of silicium, sulphur, zink, copper, iron, calcium, magnesium and potassium in smaller concentrations.
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Investigation of the production parameters, nutrient and mineral composition of mealworm (Tenebrio molitor) larvae grown on different substrates
129-133Views:226During the rearing of mealworm (Tenebrio molitor) larvae, the optimisation of the growing substrate has a particular importance. The application of the appropriate substrate is a fundamental pillar for intensive and safe production. The requirements for substrate include the lack of toxins, high nutrient and micro-macro element content. The aim of this study was the evaluation of the effects of different substrates on the production parameters, nutrient and mineral composition of the mealworm larvae. The experiment lasted through 14 days. 5 treatments were set up at, where the variable was the substrate. The test system consisted of 25 units (5 treatments and 5 replicates). 10 mealworm larvae per unit, (total of 250 larvae) were used at the beginning of the experiment. Regarding the substrates, our study included sweet potato (SP), may turnip tuber (MT) and may turnip leaf (ML). In addition to the plant by-products, wheat flour (WF) and wheat semolina (WS) were used as control substrates. Trace element uptake and production parameters of the larvae were determined at the end of the experiment. Regarding the production parameters, it can be stated that the wheat semolina (0.081 ± 0.005 g) and wheat flour (0.069 ± 0.007 g) substrate used as control gave the best results for the final body weight. In terms of plant raw materials and by-products, sweet potatoes (0.063 ± 0.007) can only be recommended as substrate, while may turnip tuber and may turnip leaf produced significantly lower final body weight results (MT=0.034 ± 0.002 g ; ML= 0.036 ± 0.002 g). The nutrient composition of the larvae was not affected by the substrate, these results confirmed the high protein and fat content reported in the literature. The results with the production parameters were contradicted by the micronutrient content. Larvae reared on may turnip leaf (ML) and tuber (MT) showed the highest values for most of the macro- and microelements (potassium, calcium, magnesium, iron, zinc, copper) tested.
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Changes of some soil chemical and microbiological characteristics in a long-term fertilization experiment in Hungary
253-265Views:288Agricultural management practices – directly or indirectly – influence soil properties.
Fertilization rates and crop rotation can strongly affect soil pH, soil nutrient supply and soil organic matter content due to the changes of microbial processes. The objective of this study was to compare the effects of different fertilization doses in monoculture and tri-culture of maize (monoculture: only maize grown since 1983, tri-culture: it is a three-year crop rotation system: pea – winter wheat – maize) on selected soil characteristics. The long-term fertilization experiments were set up in 1983 in Eastern Hungary. These experiments are situated west of Debrecen in Hajdúság loess region, on calcareous chernozem (according to WRB: Chernozems).
The test plant was maize (Zea mays L.). One-one pilot blocks were selected from monoculture and tri-culture of the long-term experiments. The observed soil samples were taken in the 30th year of the experiment, in 2013. The doses of NPK fertilizers increased parallel together, so the effects of N-, P- and K-fertilizers cannot be separated.
With the increasing fertilizer doses, the soil pH has decreased in both crop production systems and, in parallel, the hydrolytic acidity has significantly increased. A close negative correlation was proved between the pHH2O, pHKCl and hydrolytic acidity. An increased nutrient content in soil was recorded in every NPK treatment and the available phosphorus and nitrate content increased in higher proportion than that of potassium. Of the measured parameters of C-and N-cycles, fertilization has mostly had a positive effect on the microbial activity of soils. Besides the effects of fertilizer doses, correlation were looked for between soil microbiological properties. Evaluating the ratios among the measured parameters (organic carbon and microbial biomass carbon, OC/MBC ratio; carbon-dioxide and microbial biomass carbon; CO2/MBC proportion), the fertilization rate seems to be favoured by the increase of amounts of organic compounds
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The effect of nickel-contamination, nitrogen-supply and liming on the chemical composition of perennial ryegrass (Lolium perenne L.)
85-92Views:97Plant-production is determined by many production-factors. Each of these factors became subject of research-works through the years, still we state, that studying their interaction is even more important. For studying these interactions we set up a potexperiment, within that the direct effect and the interactions of four factors was inspected: soil, nitrogen-supply, nickel-loading and liming. Experiments were carried out on two soil types with extremely different characters: one was a chernozem soil with good fertility and buffering capacity, the other was a shifting sand soil with low humus-content and buffering capacity. Nitrogensupply and liming was added on two levels, while nickel on three within 12 combinations on each soil types. Plant production was cut two times within the vegetation period. The amount of production and dry matter was weighted, fractured and their element-content was measured by an ICP-detector.
Ca-content on the shifting sand soil was determined by all three factors, however the interaction between nickel-loading and liming was also significant. Nitrogen and liming increased Cauptake, that is due to appropriate nutrient-supply and improvement of a better pH-value. On the chernozem soil nitrogen and CaCO3 also increased the Ca-content. This is caused by a better nutrient supply and a higher amount of available Ca-ions.
On the shifting sand soil nickel content was increasing parallel to higher nitrogen-dosages. In presence of higher nickelamount the nickel-content of plants was also increasing, still according to liming, this increment was different. On the chernozem soil nitrogen a nickel increased Ni-uptake. However, liming also had a positive effect on Ni-content, that can be explained by the high amount on colloids in the soil, the adsorption of Ni-ions on them and in presence of liming material the replacement of Ca-and Ni-ions.
The potassium-content on the shifting sand soil was different in each liming-combination. In combinations without nickel the potassium-content of limed and not limed combinations was on the same level. In not limed combinations by adding nickel potassiumcontent was increasing, while in limed combinations no change was observed. On the chernozem soil by adding liming material the amount of uptaken potassium was decreasing, that is due to the antagonism between Ca- and K-ions. -
Microbiological preparations affecting the soil nutrient availability and growth of ryegrass in a pot experiment
49-53Views:138The effects of different bacterial fertilizers and their combinations with NPK fertilizer and wheat straw were investigated on some soil properties (chemical parameters) and on the biomass production of testplant. The applied quantities of the bacterial fertilizers were the double of the recommended dose. The experiment was set up in 2013 at the Institute of Agricultural Chemistry and Soil Science, in a three replications, in a random block design. Calcareous chernozem soil; originating from Debrecen (Látókép) was used with ryegrass (Lolium perenne L.) test plant. At the end of the experiment (after 8 week) the samples of soil and plants were determined for nitrate-nitrogen, ALsoluble phosphorus and potassium content of soil, the weight of green biomass of ryegrass per pot, the dry matter and moisture content of ryegrass. Straw treatment resulted better water and available nutrient content of soil in general. Inoculation however was not improving the biomass production over the fertilizer treatment. Interrelation with the recommended dose could be further studied.
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Influence of Foliar Treatments on the yield and quality of Sugar Beet
119-123Views:99The yield and quality of the sugar beet are mainly determined by the plant production system, thus we studied the effect of mineral fertilization. Our field trials were carried out in 2005 in Hajdúböszörmény, at two sites. We studied the effect of sulphur (Cosavet DF), copper (Kelcare Cu) and two foliar nutrients with high active agents. Treatments were replicated four times.
We took root samples at 4 week intervals, starting in August. The quality of root (sucrose, potassium, sodium and alfa-amino N content) was determined from filtrated beet broth, by an automatic beet laboratory system, called VENEMA.
We found that the crop and the sugar yield were significantly influenced by the foliar treatments -
The effect of NPK fertilization and the plant density on maize yield and bioethanol production
13-18Views:100For industrial (bioethanol) production of maize, a new production technology is needed. I tested and selected hybrids appropriate for this purpose and set up fertilization and plant density experiments. The experiment were set up on chernozem soil in 2008.
In bioethanol production, the selection of a high-yielding hybrid with high starch content, a slight reduction of N, increase of potassium, the application of the highest plant densities of the optimum interval, harvest at full maturity (when starch content is the highest compared to protein content) are of great importance. -
The irrigation influence under the soil, microclimate and plants in maize from Crişurilor Plain
180-186Views:94The paper is based on the researches carried out in the long term trial placed on the preluvosoil from Oradea in 1976, for establishing
the soil water balance. In the irrigated variant the soil moisture was determined 10 to 10 days for maintaining the soil water reserve on
irrigation depth (0 – 50 cm for wheat and bean, 0 – 75cm for maize, sunflower, soybean, sugarbeet, potato and alfalfa 1st year and 0 – 100
cm for alfalfa 2nd year) between easily available water content and field capacity. Thus, an average irrigation rate of 2560 m³/ha was used
in the 9 experimental crops. The average of the annual rainfall for the 1976 – 2008 period was of 625.0 mm. The technologies used were
correlated with the needes of the crops, such as melioration crop rotation, chemical fertilizers in accordance with the chemical export on the
yield, manure (40 t/ha) was used in potato and sugarbeet. After 33 years of the irrigation use the soil structure degree (38.62%) did not
decrease when compared to the unirrigated maize – wheat crop rotation (37.01%). Bulk density, total porosity, penetration resistance and
hydraulic conductivity have worse values than the ones in the unirrigated variant. The humus content is very close to the humus content
determined in 1976, the phosphorus and the potassium content increased very much in comparison with the initial content (117 ppm vs 22.0
ppm); (180.0 ppm vs 102 ppm). The use of the adequate fertilization system and of the irrigation water with a good qaulity did not determine
a decrease of the pH value of the soil. The irrigation determined the improve of the microclimate conditions, the increase of the plant water
consumption, yield gains very significant statistically and higher protein content of the maize grains. -
Behavior of some Hungarian wheat varieties to seed soaking in gibberellic acid under salt stress
7-17Views:202Various abiotic stresses including high salinity strongly affect seed germination. Three Hungarian wheat landraces Gamási, Fóti and Kartali have been tested for seed germination, seedling growth and chemicals parameters when seeds were soaked in gibberellic acid (GA3) at the concentrations of 0, 75 and 150 ppm and at four levels of salinity stress 0, 5, 10 and 15 dSm-1. A laboratory experiment took place at Research Institute of Nyíregyháza. Factorial Experiment based on Randomized Complete Block Design (RCBD) in four replications has been conducted. Seed soaking in GA3 before sowing significantly affected germination characteristics, seedling properties and the results of chemical analysis. The highest germination as well as K+ content of seeds were observed at seed soaking into 150 ppm GA3. However, Na+ and proline contents in seedling were decreased. The behavior of the tested Hungarian wheat varieties was genotype-dependent. Increasing salinity stress up to 15 dSm-1 significantly affected germination characteristics, seedlings parameters and chemical analysis. Fóti variety turned out to be the best at 150 ppm GA3 compared to the other two varieties. Moreover, it had the lowest Na+ and proline contents and highest K+ content.