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The effect of different fat content fish feed on the production parameters and meat quality of the hybrid striped bass
33-37Views:160The interspecific ’hybrid striped bass’ is the result of cross breeding. Their offspring have got good chances of growth and tolerate extreme water temperatures and the content of dissolute oxygen more than their parents. Furthermore, they are more resistant to diseases. Therefore, the hybrid is perfectly suitable for intensive fishery production.
Our experiment lasted for seven weeks. We analysed the indices of growth and production of the hybrid as the consequence of nurturing them with two feeds of high nutritive value during this period. We examined the content of fat and of fatty acids in the flesh of these fish. There were no considerable differences in terms of weight gain, feed conversion ratio and uneven growth between the two groups, while survival was less likely among the the fish which had consumed more fat. We demonstrated, examining the flesh of the individuals which had consumed feed containing lower fat content, that it was fattier and contained more dry matter than those that had been fed of higher fat content. Besides, the content of omega-3 and omega-6 fatty acids was higher in the fish that had consumed less lipids.
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Investigation of the effect of allithiamine-enriched feed on the poultry gut microbiome composition and resistome
149-155Views:66Over the past 20–25 years, the poultry industry has evolved into a specific protein production system. However, the stress resulting from intensive rearing practices has led to numerous negative consequences, making the optimisation of livestock gut microbiome composition crucial for mitigating these effects. Advancements in modern molecular biology methods have brought attention to the impacts of nutrients on gut microbiota. In our study, we extensively investigated the changes induced by feed formulations rich in phytonutrients on the gastrointestinal microbiota of livestock using targeted 16S rRNA amplicon sequencing. Our objective is to examine how the developed feed prototype affects the composition of core microbiomes in raised poultry, community diversity, and the resilience of complex microbial networks. We seek correlations between biological livestock and environmental samples to identify which community constituents, in what proportions and occurrences, may play a role in the development of specific diseases. Based on our measurement results, it can be asserted that allithiamine positively modulated "beneficial" community constituents. Beyond the impact of allithiamine-enriched feed rich in phytonutrients, the composition of the microbial community in the poultry gastrointestinal tract is significantly influenced by the age of the birds. Furthermore, due to the presence of multi-drug-resistant pathogens in environmental samples from livestock facilities, appropriate transmission risk management measures are of paramount importance.
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The response of sunflower hybrids to different plant densities on a chernozem soil
123-128Views:147In our experiment the reaction of six sunflower hybrids of different genotypes (NK Oktava, ES Biba, ES Diagora, ES Ballistic, EGH 8925, PR 64 H 42) towards plant density has been investigated by different fungicide treatments in the crop-year of 2011 on a chernozem soil.
In the crop-year of 2011 sunflower populations were infected by a significant Diaporthe helianthi disease. The extent of this infection was significantly enhanced by the increment of plant density. However, regarding the average of the hybrids and plant densities the two times executed fungicide treatment has decreased the infection rate by 22%. The most susceptible hybrid was the ES Biba. Contrarily, the hybrid ECH8925 proved to be the most resistant hybrid towards this
disease according to our results. According to the results of the Pearson’s correlation analysis it has been revealed that stalk breakage and Diaporthe infection stand in a very close (r=0.782**) and middle close (r=0.523**) correlation resp. with plant density. The relationship between fungucude treatments and stalk breakage, just as Diaporthe infection showed to be middle and close respectively. Our results demonstrate the role of stalk and plate diseases (among them Diaporthe) in causing stalk breakage, for we have found a close positive correlation between stalk breakage and Diaporthe infection (r=0.624**) in our analysis.
From the aspect of yield amount the optimal plant density varied between 45 000 and 55 000 plants per hectare. Fungucude treatments enabled not only the use of higher plants densities, but they had a yield increasing effect as well. In the crop-year of 2011 the highest yield (4 559 kg ha-1) on a chernozem soil has been measured in case of the hybrid ECH8925. -
Susceptibility of maize hybrids to corn smut diseas
39-42Views:142Corn smut disease is one of the most common maize diseases. In the previous years the disease lost from its importance, however nowadays it is becoming more and more important and widespread. The protection against it encounters great difficulties, therefore it is important to emphasize prevention and resistance based protection. The aim of the research was to investigate under laboratory circumstances the resistance of Hungary’s most commonly cultivated maize hybrids to corn smut disease. Twelve species were investigated. We distinguished two susceptibility groups within the hybrids based on the investigated parameters. The results are intended to assist the protection against corn smut disease and to make a suggestion for the farmers so that they could apply more environmental friendly and economical production.
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Effect of anthocyanin-rich sour cherry extract on the level of IL-8 in LPS-induced endothelial cell
27-30Views:196The anthocyanin content of the Hungarian sour cherry is remarkable. Nutraceutical and pharmaceutical effects of the anthocyanins and their role in disease prevention have been studied extensively. Endothelial cells are involved in the pathogenesis of several inflammatory diseases. The objective of this work was to investigate pure sour cherry extract on human umbilical cord vein endothelial cells (HUVECs) as an inflammatory model. HUVECs were treated with 100 ng/mL lipopolysaccharide (LPS) and 50 mg/mL sour cherry extract or M199 medium as control. The optimal concentration range of the sour cherry extract was investigated and selected based on MTT assay measuring the conversion of the tetrazolium salt to formazan by mitochondrial dehydrogenases. The level of interleukine-8 (IL-8), a pro-inflammatory cytokine, was measured in Luminex MagPlex assay. LPS treatment significantly increased the secretion of IL-8. The pure sour cherry extract was able to attenuate this increment indicating the potent anti-inflammatory effect of pure sour cherry extract. Our results emphasize that pure sour cherry extract could reduce the LPS-induced inflammatory response thereby may improve endothelial dysfunction.
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Effect of Ozone Exposure on Phytopathogenic Microorganisms on Stored Apples
9-13Views:93The aim of our study was to clarify the effect of ozone exposure on several phytopathogenic fungi on stored apple fruits under different storage conditions. The study was conducted at Bistrita, Romania, in the storehouse of an experimental apple orchard in 2002 and 2003. Two widely grown apple cultivars (‘Jonathan’ and ‘Golden Delicious’) were used. General microbial examination of the fruits was made during storage in order to identify the most important storage pathogens. Efficacy of six ozone treatments was evaluted on fruit decay caused by phytopathogenic fungi. Monthly observations (January, February, March and April) were made of the degree of decay and three measurements were assessed (disease frequency, disease intensity and degree of attack). Our results showed that the most important phytopathogenic fungi during storage was blue mold, caused by species of Penicillium. Disease frequency of apple fruits was very high on cv. ‘Jonathan’, much higher than on cv. ‘Golden delicious’. Ozone treatments (25 ppm ozone for 0.5 and 1.5 hours in November) caused significantly lower disease incidence on stored apple than all other ozone treatments. For longer storage, it seems that additional ozone treatments in February increased treatment efficacy. Cv. ‘Golden delicious’ seemed to be more resistant to storage diseases than cv. ‘Jonathan’ both on the untreated and treated fruits. The effect of the ozone treatments was also the most effective when 25 ppm ozone was applied for 0.5 and 1.5 hours in November.
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Disease incidence of shot-hole disease of plum in two training systems
107-110Views:24Of the foliar diseases of European plum, Wilsonomyces carpopilus is the most commonly occurring fungal pathogen. The aim of this two-year study was to investigate the susceptibility of ‘Čačanska lepotica’ plum variety to shot-hole disease (Wilsonomyces carpophilus) in two different training system with 4 x 1.5 m and 6 x 3 m tree spacings. The obtained results showed that the cultivar is susceptible to this disease and by the end of the vegetational period disease incident was above 50% in both years in both tree spacings. In 2018 disease incidence was higher in both spacing than in 2019, reaching almost 90% at the 4 x 1.5 m tree spacing plot. There were few significant differences between high and low density tree spacings. The results highlighted the importance of inoculum accumulation late in the season.
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Characteristics of roe deer bot fly infection in the southern part of the Great Hungarian Plain
57-60Views:133I am investigating the occurance of one of the diseases caused by parasites in plain roe deer populations, namely a botfly larvae, Cephenemyiastimulator, (Clark, 1815) ranged among the Oestridae family. Nasal botfly larvae are common roe deer parasites and are generally spread nationwide. The subject of the present survey is the processing of the data gained about roe deer bucks of the year 2009 and their infestation indices. I investigated botfly larvae in 90 bucks’ nasal and pharyngeal cavity. We have found botfly larva in 18.9% of the examined bucks (prevalence), this means 17 infected specimen. I collected 218 pieces of larvae from the samples, with a 12.8 pieces average intensity. In my investigations prevalence was lower and mean intensity was higher than in previous Hungarian researches. This was manifested both in my overall data, and in our examinations by the different age groups.
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Selection of powdery mildew resistant and susceptible grapevine genotypes with molecular markers
100-104Views:108Incorporation of competitive quality and resistance against the most important fungal diseases (powdery and downy mildew) in a cultivar is one of the most important aims of grapevine breeding. In the 20th century, the most advanced results in grapevine resistance breeding were achieved by French researchers. They used resistant cultivars in more than 30% of their growing areas. In these varieties, North American wild Vitis
species were the resistance gene sources. The discovery of immunity-like resistance of Muscadinia rotundifolia opened new perspectives in resistance breeding. M. rotundifolia harbours a dominant powdery mildew gene, providing resistance in highquality cultivars after back-crosses with V. vinifera varieties. M. rotundifolia has been involved in the Hungarian grape breeding programs since 1996, thanks to a French-Hungarian variety exchange. In addition to traditional selection methods, application of MAS (Marker Assisted Selection) based on various types of
molecular markers, can provide additional tools for these efforts. Run1 locus, responsible for powdery mildew resistance, was identified in Muscadinia rotundifolia. Molecular markers closely linked to this locus are very significant in screening progenies deriving from M. rotundifolia and V. vinifera crosses, making possible the discrimination between resistant and susceptible genotypes at DNA level. In our analyses BC5 progeny of {(M. rotundifola×V. vinifera) BC4}×Cardinal (V. vinifera) tested for powdery symptoms were analysed with PCR-RFLP (GLP1- 12P1P3) and microsatellite markers (VMC4f3.1, VMC8g9). Our results proved the applicability of the linked markers and reliability of marker assisted selection. -
Testing the antimicrobial activity of essential oils
71-74Views:131The vapor phase of some essential oils proved to have antimicrobial activity. Utilization of the vapor phase of Eos is presently understood as one of the possible alternatives to synthetic food preservatives which could be used in the future. However, testing the vapor phase of EOs against microorganisms causing food-borne diseases (e.g. Salmonella enteritidis or Staphylococcus aureus) or food spoilage is relatively new. Consequently, due to the large number of known EOs, research on their antimicrobial activity is still largely in the phase of in vitro rather than in vivo testing. Moreover, no standard and reliable method for fast screening of a wide range of samples exists. Thus, the aim of this study is to show results concerning tests of the antimicrobial activity of EOs against S. enteritidis or S. aureus, which were conducted by two modifications of the disc volatilization method we developed. The lately developed method has the potential to become widely used for fast screening of EO antimicrobial activity in the vapor phase.
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Comparison of Integrated and Conventional Production of Young Nonbearing Apple Orchards
3-5Views:76The large number of pesticide applications in apple orchards creates serious problems with pesticide residues and their side effects on beneficial organisms, the environment and human health. This is the reason behind the search for new systems for apple protection.
The investigations were made in apple orchards of the Institute of Agriculture at Kyustendil, during the period from 1997-1999. Three scab resistant cultivars grafted on rootstocks MM106 were planted in 1996. The orchard was divided into four plots. Two plots were treated as „conventionally” and the other two were treated as „integrated” according to the general principles, rules and standards of integrated apple production.
The key disease during the experimental period was powdery mildew, which can be controlled only with pruning of infected clusters and shoots during the first three years after planting. The key pests in the orchard during the nonbearing period were the green apple aphid and San Jose scale. In the integrated plant protection system, it is possible to reduce the number of insecticide treatments depending on the density of the main pests. -
Old-new disease is maize smut (Ustilago maydis)
43-46Views:184Corn smut disease (Ustilago maydis) is one of the most common maize diseases. In the previous years the disease lost some from its importance, however nowadays it turns into more and more important and widespread one. The control encounters great difficulties, therefore it should be emphasized the preventive and resistance-based protection.
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Dietary fibre prevalence and its role in human nutrition
9-13Views:203The role of dietary fibre and its impact on health is increasing. It is worth noting that their beneficial effects on the body are becoming more widely known, but their consumption is still below the recommended daily intake from food. Dietary fibre, as a class of compounds, includes a mixture of plant carbohydrate polymers, both oligosaccharides and polysaccharides. Dietary fibre has been linked to a number of diseases, as its consumption can reduce the risk of cardiovascular disease and type 2 diabetes. They influence the composition of the bacteria present in the human gut. Consuming the right quantity and quality of dietary fibre can play a role in the prevention of certain types of cancer. Nowadays, it is common to produce various fibre-enriched foods, called functional foods, because of the beneficial effects they have previously had on the body. In the present work we want to give a general description of dietary fibre and its role in the body.
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Examination of French bean on organic and conventional farming of Research Centre of Nyíregyháza
87-98Views:74This study presents the yield results of some French bean varieties in organic and conventional farming. This study presents the advantage of organic farming in environmental point of view and in nutrition. Sale of organic products is insured, there is solvent demand rather in abroad than in Hungary.
In Research Centre of Nyíregyháza had made organic farming since 1994, at present on 74 hectares.
In the first trial, variety comparison with 9 yellow podded French beans in organic and conventional farming was conducted. Varieties: Carson, Cherokee, Debreceni sárga, Goldmine, Héliosz, Minidor, Sonesta, Sundance és Unidor. The following parameters were observed: the time of emergence and flowering, number of plants per plot, plant height and flowing green harvest. We weighed yield of the standardized, un-standardized and diseased pod fractions. The results were evaluated statistically with SPSS and Excell softwares.
Emergence had all at once, but the plants of organic farming were 5 days earlier at flowering and maturity, than conventional farming.
The plants in organic place were more developed than in conventional ones. Emergence was more uniform, the growth and the number of plant were square. Significant difference was not detected in plant height between two places. Most of the varieties examined had better total yield in organic place, than in conventional ones. Deviation depended on variety. ‘Sonesta’ and ‘Debreceni sárga’ had the best yield in both places. In
conventional farming choice can be expanded with ‘Unidor’ and ‘Sundance’. In organic farming choice can be expanded with ‘Minidor’ and ‘Carson’.
In both places the Sonesta, Debreceni sárga and Unidor varieties had the most standardized yield per hectare. In organic place Carson variety had good pod yield because it was infected less by diseases.
In the another trial we studied inside content values of some varieties on organic and conventional places. The parameters were observed: dry matter-, starch-, crude fibre-, crude protein content and amino acid content.
The rates of asparagin or glutamine acid were the highest, which was followed by serin or histidine. In asparagin content was the most deviation between conventional and organic farming.
Significant differences were between varieties in dry matter-, starch-, crude fibre-, and crude protein content both on organic and conventional places. Each variety had significant differences between organic and conventional farming.
Starch had strong and negative correlation with dry matter, crude fibre and crude protein content.
Budai piaci and Minidor varieties and BU-16 variety candidate had higher starch content and lower dry matter, crude fibre and crude protein content on organic place. Sonesta variety had almost equal dry matter content on both places, but crude protein content was higher with 10% and starch content was lower with 6% on organic place, than on conventional place. Paridor variety had almost equal starch content, but it had higher dry matter and crude protein content on organic place.