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• The identification of phylloquinone in cattle samples using T.L.C. method

  75-78

  Lucian Bara

  Views:

  93

  A case of hemorrhage of unknown origin was observed in cattle and liver samples were submitted for assay of Vitamin K or
  possible cuomarol. After evaluating normal and reverse phase Thin Layer Chromatography (TLC) plates and different solvents,
  reverse phase TLC plate with indicator and methylene chloride:methanol 70:30 were chosen for direct method of detection and
  indication of Vitamin K.Gass Chromatography and Densitometry were used to quantitate the Vitamin K present in liver samples and
  confirmation of Vitamin K was done by Mass Spectroscopy.

• Age of puberty and conception rates of Hungarian Merino, (Merino x Somali) F1 and (Merino x Barbados Blackbelly) F1 ewes

  15-19

  Gergely Gyimóthy

  Nóra Vass

  Péter Balogh

  András Jávor

  András Kovács

  Views:

  221

  The aim of our examination was to detect the puberty of the Hungarian Merino and its hairsheep crossbred ewes, and also to determine their average conception rates after having ram exposed in May and June. Hormon diagnostation was used to evaluate the exact date of the very first ovulation. Ultrasound technique was used for checking the conception rates of ewes. The pregnancy detection was applied after a month of the date of taking off the ram.
  We evaluated the ages of ewes at puberty. Statistical deviation and standard error were calculated. The results proved the Hungarian Merino x Somali (N=10), ((x ± s) = 173 ± 43) and also the Hungarian Merino x Barbados Blackbelly crossbred genotypes (N=7), ((x ± s) = 186 ± 19) have their puberty much earlier as compared to the fullbred Hungarian Merino ewes (N=10), ((x ± s) = 231 ± 95). We experienced the highest conception rate in the group of the (Hungarian Merino x Somali) F1 ewes after exposing the ram in the „out-of season” period of time.

• Investigation of the milk composition of three Tsigai sheep varieties, and the detection of cazein fractions of sheep milk by Reversed-Phase HPLC

  13-22

  Ildikó Bajúsz

  Views:

  111

  The measurements started in 2005, the author used the milk of 3 types of Tsigai Sheep that are suitable for milking, they examined the milk composition, the possible differences between the milk types, the coagulation characteristics, cheese processing, yielding characteristics and the transfer of solid content into cheese. From the large number of measurements, the results of milk composition examinations are described.
  During the research the author examined the milk coagulation property of three types of Tsigai Sheep QTS 25 is a substance investigating instrument, and its primary mission is the analysis of the substance profile. The advantage of this instrument is the representation of the survey graphs from which the part of the substance characteristics could be directly read off. By the help of the applied mathematical and statistical operations we tried to establish connection between the congealment-hardness and the specialty of the different types of Tsigai Sheep.
  In 2006 the author began the identification and qualification of the major sheep milk proteins by RP- HPLC method. She used a scientific article written by Bordin et al. The method is able to separate and quantify the seven major proteins. However Bordin’s procedure was developed using bovine milk, the author adapted it to sheep milk.

• Spectral analysis of stress symptoms caused by apple powdery mildew (Podosphaera leucotricha

  83-88

  Attila Nagy

  Péter Riczu

  János Tamás

  Views:

  243

  An orchard can be examined on the basis of spectral data, using methods with which the reflected radiation can be divided into a large number of (several hundreds) small spectral channel (some nm). Calculated on the basis of such hyperspectral data from different index numbers the water supply of foliage conditions can be well characterized.

  The research site is an intensive apple orchard, which located in University of Debrecen, Agricultural Sciences Centre, Farm and Regional Research Institute at Pallag. During my experiments the preliminary evaluation of spectral, non-invasive measurement method are carried out for detecting stress symptoms caused by Podosphaera leucotricha.

  Based on the results narrow band greenness indices (NDVI705, mNDVI705, mSR705 and REP) can be used for determination of diseased canopy and for the detection of stress symptoms of Podosphaera leucotricha,. These statements can be utilized in precision plant protection systems, since it can be a basis for such integrated active sensors with LED or laser light source, measuring reflectance at the certain spectral range, which can facilitate real time status assessment of orchards and can control precision fungicide utilization.