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Mitochondrial DNA-based diversity study of Hungarian brown hares (Lepus europaeus Pallas 1778)
23-29Views:146The brown hare being an important game species which is widespread across the European continent has been in focus of many population genetic studies. However only a few comprising researches can be found on the diversity of Central-European populations.
The aim of our large scale long term ongoing study is to fill this gap of information on the species by describing the genetic history and structure of the brown hare populations of the area using both mitochondrial DNA markers and genomic skin and hair colour regulating genes.
This article gives forth a part of our results concerning the mitochondrial DNA diversity of Hungarian brown hares based on amplification of a 512 bp long D-loop sequence. N=39 tissue or hair samples have been collected from 15 sampling sites on the Hungarian Great Plain. We have described a high level of haplotype diversity (Hd=0.879±0.044) based on a 410 bp alignment of our sequences. We have found 17 haplotypes within our sample set with the nucleotid diversity of π=0.01167±0.0022. Our ongoing research shows high genetic diversity for the brown hare in the studied region and a second alignment with 156 sequences downloaded from GenBank indicates a geographic pattern of haplotypes among the studied populations though these results need confirmation by our further analyses.
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The Effect of Year and Irrigation on the Yield Quantity and Quality of the Potato
12-16Views:101In Hungary, the growing area of potato area reduced dramatically in the last few decades, additionally we are lagging behind the Western European countries as regards yields and the competitiveness of production is further decreased by the great alternation in yields from year to year, the unpredictable market conditions, bad consumption habits and many times unfortunately the lack of quality products.
The ecological and climatic conditions of Hungary are not everywhere suitable for potato, in the area of Debrecen the amount of rainfall was lower, and the monthly average temperature was higher than the requirement of potato in its growing season in 2002 and 2003.
The experiment was carried out at the experimental site of the University of Debrecen, Farm and Regional Research Institute, at Látókép. In our experiment we examined the yield and some quality parameters of 8 and 9 medium-early varieties in large parcels in 2002 and 2003 respectively. Out of the examined varieties 3 are of Dutch, and 6 are of Hungarian breeding.
The experiment was set up on 49.5 m2 parcels on calcareous chernozem soil after winter wheat as a forecrop in both years. The 9 varieties were examined in 4 repetitions in randomized blocks, out of which two repetitions were irrigated, and two were non-irrigated.
We examined the yields of the varieties, the distribution of tubers according to size and their percentages, and the changes in specific parameters of quality and inner content due to irrigation. We studied the dry matter content, the starch content, the under-water mass, the amount of reducing sugars, the colour index of frying and the element contents of tubers.
Summing up, it can be stated that among the agrotechnical year effect, variety and irrigation factors have considerable impact on potato yield quality and quantity. On the basis of our results, it can be stated that in potato production variety should be chosen in accordance with the aim of production and technology should be adapted to that specific variety. -
Effect of Irrigation on the Yield and Quantity of Potato Varieties
53-61Views:97In Hungary, the growing area of potato area dropped dramatically in the last few decades. Additionally not only are we lagging behind Western European countries as regards yields, but the competitiveness of production is further decreased by the great alternation in yields from year to year, unpredictable market conditions, poor consumption habits and, often the lack of quality products.
The experiment was carried out at the experimental site of the University of Debrecen, Farm and Regional Research Institute, at Látókép. In our experiment, we examined the yield and some quality parameters of 9 medium-early varieties in large parcels. Of the examined varieties, 3 are of Dutch, and 6 are of Hungarian breeding.
The experiment was set up in 2003 and 2004, in two years of significantly different precipitation, on 50 m2 parcels on calcareous chernozem soil after winter wheat as a forecrop in both years. The 9 varieties were examined in 4 repetitions in randomized blocks, from which two repetitions were irrigated, and two were non-irrigated.
We examined the yields of the varieties, the distribution of tubers according to size and their percentages, and the changes in specific parameters of quality and inner content due to irrigation. We studied the dry matter content, the starch content, the underwater mass, the amount of reducing sugars, and the colour index of frying of the tubers.
In Summary, it can be stated that among the agrotechniques, year effect, variety and irrigation factors have considerable impact on potato yield quality and quantity. However the effect of irrigation depends on the crop year. In a draughty year, like in 2003, irrigation could increase the yield by 10%, while in a more favourable wet year, the improving effect of irrigation was low. -
Investigations on Mud on Heavy Metal Contaminated Flood-Plain of Tisza
96-101Views:111At the beginning of the year 2000 subsequently to a mine accident high heavy metal content mud entered the catchment area of the Tisza and was transported through the whole Hungarian section of the river. The majority of the heavy metals had been bounded to the floating sediment that was deposited on the flood-plain soil during flood forming a new, 5-10 cm thick layer. In the mud samples collected after the flood there was a clearly visible dark grey layer with significantly higher heavy metal content that was formed by the pollution wave and it was sorruonded by a light layer. The upper layer of flood-plain soils are formed from this mud layer during the soil development process, so the amount of Lakanen-Erviö soluble heavy metals that correlate with bioavailable heavy metal content was examined as well. In this case only the lead content was significantly higher in the dark layer.
New mud samples were collected after the 2001 flood. Separate layers could not have been identified, their colour was similar to those of the previous year’s light layers’. Comparing to this light layer the total Zn and Lakanen-Erivö soluble metal content was significantly lower in the mud samples of the year 2001. While the proportions of total and Lakanen-Erviö soluble metal concentrations were equal in both of the layers regarding the elements, these ratios have significantly changed next year regarding Pb and Zn: the amount of Lakanen-Erviö soluble metals considerably decreased.
As a result of sequential extraction the heavy metal content was rather low in the water soluble and exchangeable and NaOH-soluble fractions, so heavy metals found in the mud could be released in greater amount only in case of a heavy acidification. -
Effect of year and irrigation on yield and quantity of different potato varieties
7-14Views:84dramatically in the last few decades. We are lagging behind Western European countries as regards yields. The ecological conditions are not favourable for potato, but the low yields other causes: the use of non-suitable seed, the low level of irrigation and nutrient supply and out of date machinery. The competitiveness of production is further decreased by the great alternation in yields from year to year, unpredictable market conditions, poor consumption habit, and often the lack of quality products.
The experiment was carried out at the experimental site of the University of Debrecen, Farm and Regional Research Institute, at Látókép. In our experiment, we examined the yield and selected quality parameters of 9 medium-early varieties in large parcels. Of the examined varieties, 3 are of Dutch, and 6 are of Hungarian breeding.
The experiment was set up in 2004 and 2005, on 50 m2 parcels on calcareous chernozem soil after winter wheat (2004) and two rowed barley (2005) as a forecrop. The varieties were examined in 4 repetitions in randomized blocks, of which two repetitions were irrigated, and two were non-irrigated. We examined the yields of the varieties, the distribution of tubers according to size and their percentages and changes, and selected parameters of quality and chemical composition due to irrigation. We studied the dry matter content, the starch content, the underwater mass, the amount of reducing sugars, and the colour index of frying of the tubers.
Summary, it can be stated that among the agrotechniques, year effect, variety and irrigation factors have considerable impact on potato yield quality and quantity. However, the impact of irrigation depends on the year effect. -
Effect of the media on morphology of Cryphonectria parasitica (Murr.) Barr isolates and their Vegetative Compatibility Groups
38-42Views:159The most dangerous pathogen for the European and American chestnuts is the blight fungus Cryphonectria parasitica (Murrill) Barr. Short after its introduction a big number of chestnut trees were destroyed on the infested area. The control could be really complicated, because of the numerous vegetative compatibility groups of the fungus. There is a type that carries a mycovirus viz. hypovirus in the cytoplasm. We are able to control effectively this pathogen by using mycovirus-carrying strains (called hypovirulent fungal strains also). In laboratory it is easy to multiply the virulent and the hypovirulent strains of the fungus but do not easy to differentiate colonies visually on simple PDA medium. During our research, we tested different types of media, based on potato and chestnut bark extract respectively. It was observed that on potato medium the virulent strains produce more orange pigments. So it is more easy to differentiate virulent or hypovirulent isolates of chestnut blight fungus based on colony colour and morphology.
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Experiment of quality properties of dehydrated fruits
7-15Views:106The lyophilization is the joint application of freezing and drying. It is an up-to-date conserving procedure, the point of which is that the humidity existing in the frozen humid material is transferred from the solid state directly into the gaseous state at a temperature below 0 oC under vacuum. Out of the procedures applied nowadays, this is the most tolerant drying process.
With regard to the high investment and operational costs, freeze drying is applied only for valuable, heat-sensitive materials when the technological aim is to preserve such properties as aroma, taste and colour as well as such components as proteins and vitamins. This procedure is suitable for drying and conserving certain foodstuffs, stimulants, organic chemicals, medicines and similar sensitive and valuable materials.
In our institute, we have been conducting freeze drying experiments with regional fruits and vegetables since the year 2005. During the first phase, we examined the heat- and material transfer as well as the abstraction of humidity, while during the second phase we analysed the rehydration ability and nutrient content of the freeze-dried materials as compared to those dried with the method of convection. Moreover we have conducted penetration measurements with a portable hardness tester.
To sum up the results gained so far, we can state that the quality of the lyophilized materials is better than those dried in the traditional way. It originates partly in the fact that the temperature and pressure applied for the freeze drying are smaller and the drying period is far longer than for the convection drying.
In contrast to convection-dried materials, freeze dried materials set in close to their original water-content, keep their original shape and size after being rehydrated. The reason of it the porous, spongy structure (flexible cell wall) of the lyophilized products which is able to take up moisture quickly. In addition, the lyophilized products can be rehydrated faster than those dried in the traditional way.
Regarding the results of the chemical analyses, the following conclusion can be drawn: the vacuum freeze drying results a small decrease of nutrient content and nutritive value for the lyophilized products.
The results of the hardness tests support the statement that the majority of agricultural materials cannot be considered as an ideal flexible body, because during the experiment the flexibility coefficient changed when going from the surface of the material inwards. In addition, the penetration tests also confirm that the surface of the convection-dried vegetables is at least 1.5-3 times harder than that of the freeze-dried products. The reason of it that it takes place during the drying denaturation processes.
The article summarizes the results of our research work listed above, in accordance with our experiments conducted by using the characteristic fruits (apple, plum) of the Nyírség Region. -
Studies on the Suitability of Different Mould Media Compositions for the Mycological Evaluation of Hay Samples
34-38Views:74t evaluating mould contamination of hay samples in more than the half of the cases Mucor spp. (and Trichoderma spp. in a smaller extent) overgrew slower developing moulds, spoiling the assessment of total mould CFU and the detection of fastidious organisms, among others the toxinogenic Fusaria. In parallel microbiological evaluation of hay samples comparing to the ISO 7954 medium as reference a) the overgrowth inhibiting effect of Rose Bengal (ISO 7954-RB); b) the combined inhibiting effect of Rose Bengal and Dichloran (dichloro-mononitroaniline) in DRBC; c) the inhibition of Dichlorane in ISO 7954 (ISO 7954-DC); d) the lowering of nutrient levels to 1/4 (1/4 N ISO 7954, 1/4 N-DRBC and 1/4 N-DC); e) the inhibiting effect of dinitro – salicylic – acid (DSA), a reducing sugar binding compound, as a potential growth inhibitor (RBC-DSA) were studied. The results showed, that 1) MSZ-ISO 7954 medium codified by the official method was unsuitable for the detemination of mould count and for the detection of toxinogic spp. in hay samples. In half of the cases the overgrowth of Mucor has spoiled CFU enumeration and recognition of toxinogenic moulds; 2) Inhibitor supplemented DRBC medium (King et al., 1979) enabled early CFU enumeration by uniforming colony sizes and by efficient suppression of Mucor, but the pink background colour of the medium was disturbing the observation of tints of conidia, which were characteristical to toxinogenic moulds like Fusaria. The hypha-staining property of Rose Bengal did not prove very important; 3) According to the recent stage of our studies, the ISO 7954-Dichloran combination can be recommended for the mycological evaluation of hays and dried roughages. CFU can be enumerated early, Mucor suppressed in the same extent as with DRBC and colours are easily observable; 4) 2-4-Dinitrosalicylic-acid (DSA) proved unsuitable as inhibitor, for its poor efficiency and for its intense yellow background coulour.
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Black walnut as a valuable stand-forming tree species in Hungary – a growing technology review
129-132Views:163In Hungary black walnut (Juglans nigra L.) is one of the most valuable exotic tree species, mainly because of its excessively liked wood for furniture industry, that has fine tissue and singular colour. Generally it is established by seeding, it can be regenerated by coopice shoot well, but not by seeding in natural way. Black walnut crops regurarly from the age of 25–30 years and its rotation age is 75–85 years. Prosperous decorative shape of stem allows its using in tree plantings out of forests, too. In the future its role can increase mainly on good.
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Comparing skull formation of the Hungarian (Hortobágy) Zackel sheep breed by geometrics morphometrics
29-33Views:144This work seeks to explore the morphological changes of the Hungarian (Hortobágy) Zackel sheep's skull, which occurred in the past 50–70 years. In this study, we compared individuals skull forms by geometric morphometric methods. The origin of the breed is not known, we do not know when entering the Carpathian Basin. Therefore, the comparison involved the only known early archaeological findings. We have shown that there is no difference between each period colour variations, but over time change has occurred in the skull formation of the breed.
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Inland and foreign Erwinia amylovora isolates by carbohydrate utilization
29-33Views:78Fire blight, a plant disease caused by the bacterium Erwinia amylovora, produces serious losses in apple and pear orchards all over the world. Since the appearance of fire blight in Hungary (Hevesi, 1996) Erwinia amylovora isolates were collected in different years, from different hosts and areas in order to establish gene bank for future epidemiological studies. We had isolates from foreign countries as well. The aim of our research was to compare all of the Hungarian and foreign isolates by carbohydrate utilization.
In our experiments effect of carbohydrates on E. amylovora multiplication was determined using API 50 CH strip (bioMérieux, France). By the API 50 CH strip method we checked a number of unstudied carbohydrates. The results of the tests shows colour changes. Based on utilization of 49 carbohydrates of API 50 CH kit by E. amylovora isolates, two groups of carbohydrates can be defined: “Utilized” - and “Not utilized” carbohydrates. All isolates utilized 20 different carbohydrates after 164 hour incubation. Conversely, isolates also could be divided into four groups (1, 2, 3, 4) by arbutin and raffinose utilization. In group 1.-isolates utilize arbutin; 2.- utilize raffinose; 3.- utilize both arbutin and raffinose; 4. -utilize neither arbutin nor raffinose. Presumably carbohydrate content of nectar could play an important role on invasion of the (E. amylovora) bacterium via flower.
It could be concluded that the carbohydrate utilization – completed with genetic analysis – can be used for characterization of Erwinia amylovora isolates. -
The Fattening and Slaughtering Examination at the Hungarian Racka Sheep
37-42Views:91Fattening and slaughtering characteristics of both types (black and white) of the Hungarian racka sheep were investigated at the site of the National Institute for Agricultural Quality Control in Atkár. The Hungarian merino, the dairy cigaja and the British milksheep were applied as control groups. 10 animals were examined from both sexes in each genotype. The examination was carried out according to the principles set in the Codex for sheep breeding. During the test acute diarrhoea occurred in each control group. However, only few lambs were excluded from the test from the British milksheep and the Hungarian merino groups because of significant changes in their condition. Consequently, the results obtained were not significantly influenced. No symptoms of any illness could be recognised in the Hungarian racka sheep, though. The significance of the difference between the average of the examined groups were checked by T-tests.
Daily gain was significantly less compared to the control groups, except for the white racka males. The lambs starving for 24 hours before slaughtering weighed 25.3-30.0 kgs on average. The black and white racka females weighed the least while the Hungarian merino females weighed the most. The quantity of abdominal and kidney fat was significant in the case of our native females. The quantity of abdominal fat was significantly bigger compared to each control group. On comparing the investigated groups it turned out that the white racka males had the biggest skin weight while the white Hungarian racka females had the smallest, that is the two extremes were recognised in the same colour (white) of one bred. On qualifying the slaughtered animals females had better results than males in each bred; the racka sheep were better than the dairy cigaja but were worse than the Hungarian merino and British sheep groups.
The huge differences in the carcasses of the groups were seen clearly when chopping. Examining the leg, it was noticed that the native groups proved to be smaller than the control groups, except for the black racka males. As for the short loin, the Hungarian racka and the Hungarian merino sheep proved to be the best, whereas the dairy cigaja and the British milk males had much smaller ones. The results gained when investigating the rack were similar to those of the short loin. As far as the rib and shoulder are concerned, the black racka females and the dairy cigaja females had the worst results. After chopping, the right side was also boned in every group. The small proportion of bone in the case of racka sheep was easily noticeable that resulted in the fact that the white racka females had the highest quantity of short loin compared to the other groups. When examining the back quarter, the bone proportions of the 4 racka groups proved to be significantly less than those of the control groups but it was not true for the meat proportions. As for the front quarter meat, the racka males were prominent in the great shoulder meat and in the small proportion of bone. The quantity of the first quarter bone was the least in the case of racka sheep and was the biggest for dairy cigajas. This difference proved to be significant in most cases.
In short, the Hungarian racka groups had the best results in meat quantity, which was especially significant compared to the dairy cigaja sheep. However, the quantity of external fat increased.