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Effect of year and irrigation on yield and quantity of different potato varieties
7-14Views:369dramatically in the last few decades. We are lagging behind Western European countries as regards yields. The ecological conditions are not favourable for potato, but the low yields other causes: the use of non-suitable seed, the low level of irrigation and nutrient supply and out of date machinery. The competitiveness of production is further decreased by the great alternation in yields from year to year, unpredictable market conditions, poor consumption habit, and often the lack of quality products.
The experiment was carried out at the experimental site of the University of Debrecen, Farm and Regional Research Institute, at Látókép. In our experiment, we examined the yield and selected quality parameters of 9 medium-early varieties in large parcels. Of the examined varieties, 3 are of Dutch, and 6 are of Hungarian breeding.
The experiment was set up in 2004 and 2005, on 50 m2 parcels on calcareous chernozem soil after winter wheat (2004) and two rowed barley (2005) as a forecrop. The varieties were examined in 4 repetitions in randomized blocks, of which two repetitions were irrigated, and two were non-irrigated. We examined the yields of the varieties, the distribution of tubers according to size and their percentages and changes, and selected parameters of quality and chemical composition due to irrigation. We studied the dry matter content, the starch content, the underwater mass, the amount of reducing sugars, and the colour index of frying of the tubers.
Summary, it can be stated that among the agrotechniques, year effect, variety and irrigation factors have considerable impact on potato yield quality and quantity. However, the impact of irrigation depends on the year effect. -
The Effect of Mg Foliage Fertilization on Beetroot (Beta vulgaris ssp. esculenta var. rubra) Morphology, Quality Traits and their Relations
67-72Views:260Mg-treatment increased solid soluble content in roots probably due to the larger assimilation area.
The applied foliage fertilization affected inner colour intensity and uniformity in roots positively.
The 2% Mg-sulphate solution affected pigment quantity favourably depending on varieties.
Close correlation (r=0,71) was found between the yellow (BX) and red (BC) pigments which indicates a similar biosynthetic pathway of the two compounds.
The Mg-sulphate foliage fertilization improves quality considerably but the individual reaction of varieties needs further studies. -
Mitochondrial DNA-based diversity study of Hungarian brown hares (Lepus europaeus Pallas 1778)
23-29Views:352The brown hare being an important game species which is widespread across the European continent has been in focus of many population genetic studies. However only a few comprising researches can be found on the diversity of Central-European populations.
The aim of our large scale long term ongoing study is to fill this gap of information on the species by describing the genetic history and structure of the brown hare populations of the area using both mitochondrial DNA markers and genomic skin and hair colour regulating genes.
This article gives forth a part of our results concerning the mitochondrial DNA diversity of Hungarian brown hares based on amplification of a 512 bp long D-loop sequence. N=39 tissue or hair samples have been collected from 15 sampling sites on the Hungarian Great Plain. We have described a high level of haplotype diversity (Hd=0.879±0.044) based on a 410 bp alignment of our sequences. We have found 17 haplotypes within our sample set with the nucleotid diversity of π=0.01167±0.0022. Our ongoing research shows high genetic diversity for the brown hare in the studied region and a second alignment with 156 sequences downloaded from GenBank indicates a geographic pattern of haplotypes among the studied populations though these results need confirmation by our further analyses.
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Experiment of quality properties of dehydrated fruits
7-15Views:257The lyophilization is the joint application of freezing and drying. It is an up-to-date conserving procedure, the point of which is that the humidity existing in the frozen humid material is transferred from the solid state directly into the gaseous state at a temperature below 0 oC under vacuum. Out of the procedures applied nowadays, this is the most tolerant drying process.
With regard to the high investment and operational costs, freeze drying is applied only for valuable, heat-sensitive materials when the technological aim is to preserve such properties as aroma, taste and colour as well as such components as proteins and vitamins. This procedure is suitable for drying and conserving certain foodstuffs, stimulants, organic chemicals, medicines and similar sensitive and valuable materials.
In our institute, we have been conducting freeze drying experiments with regional fruits and vegetables since the year 2005. During the first phase, we examined the heat- and material transfer as well as the abstraction of humidity, while during the second phase we analysed the rehydration ability and nutrient content of the freeze-dried materials as compared to those dried with the method of convection. Moreover we have conducted penetration measurements with a portable hardness tester.
To sum up the results gained so far, we can state that the quality of the lyophilized materials is better than those dried in the traditional way. It originates partly in the fact that the temperature and pressure applied for the freeze drying are smaller and the drying period is far longer than for the convection drying.
In contrast to convection-dried materials, freeze dried materials set in close to their original water-content, keep their original shape and size after being rehydrated. The reason of it the porous, spongy structure (flexible cell wall) of the lyophilized products which is able to take up moisture quickly. In addition, the lyophilized products can be rehydrated faster than those dried in the traditional way.
Regarding the results of the chemical analyses, the following conclusion can be drawn: the vacuum freeze drying results a small decrease of nutrient content and nutritive value for the lyophilized products.
The results of the hardness tests support the statement that the majority of agricultural materials cannot be considered as an ideal flexible body, because during the experiment the flexibility coefficient changed when going from the surface of the material inwards. In addition, the penetration tests also confirm that the surface of the convection-dried vegetables is at least 1.5-3 times harder than that of the freeze-dried products. The reason of it that it takes place during the drying denaturation processes.
The article summarizes the results of our research work listed above, in accordance with our experiments conducted by using the characteristic fruits (apple, plum) of the Nyírség Region. -
A simple method for preparing elemental selenium nano- coating inside a silicone surface
35-43Views:567Selenium nanoparticles (SeNPs) with a bright red colour have aroused worldwide attention due to their unique properties in selenium supplementation because of their low toxicity and favourable bioavailability. A simple method was developed for making a red selenium nanolayer on the inner surface of Polyvinyl chloride (PVC) and silicone tube. The selenium nanoparticles were produced by the reaction of sodium selenite and ascorbic acid. Red amorphous selenium nanoparticles have been successfully synthesized by the reaction of 500 mg dm-3 Se (sodium selenite) solution with 10 g dm-3 ascorbic acid solution at room temperature, and morphology was confirmed by X-ray diffraction analysis (XRD). The coating density was compared on PVC and silicone surfaces by using Scanning Electron Microscopy (SEM) and Energy-dispersive X-ray (EDS) analysis. The nanolayer with about 16 µm thickness on the silicone surface significantly evenly distributed compared to the PVC surface. The selenium coated silicone tube could be a good source of selenium for a continuous, low-level selenium supplementation of farm animals via drinking water.
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Effect of the media on morphology of Cryphonectria parasitica (Murr.) Barr isolates and their Vegetative Compatibility Groups
38-42Views:477The most dangerous pathogen for the European and American chestnuts is the blight fungus Cryphonectria parasitica (Murrill) Barr. Short after its introduction a big number of chestnut trees were destroyed on the infested area. The control could be really complicated, because of the numerous vegetative compatibility groups of the fungus. There is a type that carries a mycovirus viz. hypovirus in the cytoplasm. We are able to control effectively this pathogen by using mycovirus-carrying strains (called hypovirulent fungal strains also). In laboratory it is easy to multiply the virulent and the hypovirulent strains of the fungus but do not easy to differentiate colonies visually on simple PDA medium. During our research, we tested different types of media, based on potato and chestnut bark extract respectively. It was observed that on potato medium the virulent strains produce more orange pigments. So it is more easy to differentiate virulent or hypovirulent isolates of chestnut blight fungus based on colony colour and morphology.
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The analysing of value measuring features of Hungarian speckled hen
17-23Views:158Our College has been dealing with the crossbreeding of two Hungarian speckled hen breeds, the bare-neck and the featheredneck, to maintain the breeds since 1977. Apart from the gene preservation, we endeavour to find the best way for the production-purpose utilisation of the speckled hen stock. On the basis of our experiments the laying hens can be used in small-scale egg production. As for meat production, these pure-breds cannot compete with the various types of the colour-feathered hybrid constructions available in Hungary. On the basis of the results of the cross-breeding with cock-lines of various hybrid types for direct production purposes we can conclude that the Hungarian speckled hen is suitable as a mother line to produce meat-type chickens in alternative breeding systems.
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Black walnut as a valuable stand-forming tree species in Hungary – a growing technology review
129-132Views:413In Hungary black walnut (Juglans nigra L.) is one of the most valuable exotic tree species, mainly because of its excessively liked wood for furniture industry, that has fine tissue and singular colour. Generally it is established by seeding, it can be regenerated by coopice shoot well, but not by seeding in natural way. Black walnut crops regurarly from the age of 25–30 years and its rotation age is 75–85 years. Prosperous decorative shape of stem allows its using in tree plantings out of forests, too. In the future its role can increase mainly on good.
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Comparing skull formation of the Hungarian (Hortobágy) Zackel sheep breed by geometrics morphometrics
29-33Views:347This work seeks to explore the morphological changes of the Hungarian (Hortobágy) Zackel sheep's skull, which occurred in the past 50–70 years. In this study, we compared individuals skull forms by geometric morphometric methods. The origin of the breed is not known, we do not know when entering the Carpathian Basin. Therefore, the comparison involved the only known early archaeological findings. We have shown that there is no difference between each period colour variations, but over time change has occurred in the skull formation of the breed.
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Inland and foreign Erwinia amylovora isolates by carbohydrate utilization
29-33Views:165Fire blight, a plant disease caused by the bacterium Erwinia amylovora, produces serious losses in apple and pear orchards all over the world. Since the appearance of fire blight in Hungary (Hevesi, 1996) Erwinia amylovora isolates were collected in different years, from different hosts and areas in order to establish gene bank for future epidemiological studies. We had isolates from foreign countries as well. The aim of our research was to compare all of the Hungarian and foreign isolates by carbohydrate utilization.
In our experiments effect of carbohydrates on E. amylovora multiplication was determined using API 50 CH strip (bioMérieux, France). By the API 50 CH strip method we checked a number of unstudied carbohydrates. The results of the tests shows colour changes. Based on utilization of 49 carbohydrates of API 50 CH kit by E. amylovora isolates, two groups of carbohydrates can be defined: “Utilized” - and “Not utilized” carbohydrates. All isolates utilized 20 different carbohydrates after 164 hour incubation. Conversely, isolates also could be divided into four groups (1, 2, 3, 4) by arbutin and raffinose utilization. In group 1.-isolates utilize arbutin; 2.- utilize raffinose; 3.- utilize both arbutin and raffinose; 4. -utilize neither arbutin nor raffinose. Presumably carbohydrate content of nectar could play an important role on invasion of the (E. amylovora) bacterium via flower.
It could be concluded that the carbohydrate utilization – completed with genetic analysis – can be used for characterization of Erwinia amylovora isolates. -
Investigations on Mud on Heavy Metal Contaminated Flood-Plain of Tisza
96-101Views:788At the beginning of the year 2000 subsequently to a mine accident high heavy metal content mud entered the catchment area of the Tisza and was transported through the whole Hungarian section of the river. The majority of the heavy metals had been bounded to the floating sediment that was deposited on the flood-plain soil during flood forming a new, 5-10 cm thick layer. In the mud samples collected after the flood there was a clearly visible dark grey layer with significantly higher heavy metal content that was formed by the pollution wave and it was sorruonded by a light layer. The upper layer of flood-plain soils are formed from this mud layer during the soil development process, so the amount of Lakanen-Erviö soluble heavy metals that correlate with bioavailable heavy metal content was examined as well. In this case only the lead content was significantly higher in the dark layer.
New mud samples were collected after the 2001 flood. Separate layers could not have been identified, their colour was similar to those of the previous year’s light layers’. Comparing to this light layer the total Zn and Lakanen-Erivö soluble metal content was significantly lower in the mud samples of the year 2001. While the proportions of total and Lakanen-Erviö soluble metal concentrations were equal in both of the layers regarding the elements, these ratios have significantly changed next year regarding Pb and Zn: the amount of Lakanen-Erviö soluble metals considerably decreased.
As a result of sequential extraction the heavy metal content was rather low in the water soluble and exchangeable and NaOH-soluble fractions, so heavy metals found in the mud could be released in greater amount only in case of a heavy acidification. -
N-Phenyl-Phthalamic Acid and Fertilization Effects on Flowering, Fruit Set and Fruit Quality of Apple (Malus domestica Borkh.)
24-28Views:323On seven apple cultivars (Gala Must, Gloster, Granny Smith, Idared, Jonagold, Jonathan Csány 1, Mutsu), the authors studied the influence of N-phenyl-phthalamic (PPA) acid and fertilization on flowering, fruit set and fruit quality in the years 2003-2004. The research results showed that PPA application extended the flowering time of the most cultivars. Fruit set of apple cultivars increased in many cases when regulator was applied. The additional nutrient supply could increase the fruit set too. The increase of fruit set increased the number of fruit per tree. Quality parameters determined by fertilization and fruit number per tree. For example, when N-phenyl-phthalamic acid was applied without fertilization the fruit number per tree increased considerably however, it caused a considerable frittering away. The reason for this is that fruit set was high and fruit received not enough nutrition for growing up. The skin colour of fruits was decreased in several cases by the treatments. In one respect the enlarged fruit number was shaded the others and the fertilization increased the vegetative shading leaf area.
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Effect of Irrigation on the Yield and Quantity of Potato Varieties
53-61Views:362In Hungary, the growing area of potato area dropped dramatically in the last few decades. Additionally not only are we lagging behind Western European countries as regards yields, but the competitiveness of production is further decreased by the great alternation in yields from year to year, unpredictable market conditions, poor consumption habits and, often the lack of quality products.
The experiment was carried out at the experimental site of the University of Debrecen, Farm and Regional Research Institute, at Látókép. In our experiment, we examined the yield and some quality parameters of 9 medium-early varieties in large parcels. Of the examined varieties, 3 are of Dutch, and 6 are of Hungarian breeding.
The experiment was set up in 2003 and 2004, in two years of significantly different precipitation, on 50 m2 parcels on calcareous chernozem soil after winter wheat as a forecrop in both years. The 9 varieties were examined in 4 repetitions in randomized blocks, from which two repetitions were irrigated, and two were non-irrigated.
We examined the yields of the varieties, the distribution of tubers according to size and their percentages, and the changes in specific parameters of quality and inner content due to irrigation. We studied the dry matter content, the starch content, the underwater mass, the amount of reducing sugars, and the colour index of frying of the tubers.
In Summary, it can be stated that among the agrotechniques, year effect, variety and irrigation factors have considerable impact on potato yield quality and quantity. However the effect of irrigation depends on the crop year. In a draughty year, like in 2003, irrigation could increase the yield by 10%, while in a more favourable wet year, the improving effect of irrigation was low. -
Studies on the Suitability of Different Mould Media Compositions for the Mycological Evaluation of Hay Samples
34-38Views:163t evaluating mould contamination of hay samples in more than the half of the cases Mucor spp. (and Trichoderma spp. in a smaller extent) overgrew slower developing moulds, spoiling the assessment of total mould CFU and the detection of fastidious organisms, among others the toxinogenic Fusaria. In parallel microbiological evaluation of hay samples comparing to the ISO 7954 medium as reference a) the overgrowth inhibiting effect of Rose Bengal (ISO 7954-RB); b) the combined inhibiting effect of Rose Bengal and Dichloran (dichloro-mononitroaniline) in DRBC; c) the inhibition of Dichlorane in ISO 7954 (ISO 7954-DC); d) the lowering of nutrient levels to 1/4 (1/4 N ISO 7954, 1/4 N-DRBC and 1/4 N-DC); e) the inhibiting effect of dinitro – salicylic – acid (DSA), a reducing sugar binding compound, as a potential growth inhibitor (RBC-DSA) were studied. The results showed, that 1) MSZ-ISO 7954 medium codified by the official method was unsuitable for the detemination of mould count and for the detection of toxinogic spp. in hay samples. In half of the cases the overgrowth of Mucor has spoiled CFU enumeration and recognition of toxinogenic moulds; 2) Inhibitor supplemented DRBC medium (King et al., 1979) enabled early CFU enumeration by uniforming colony sizes and by efficient suppression of Mucor, but the pink background colour of the medium was disturbing the observation of tints of conidia, which were characteristical to toxinogenic moulds like Fusaria. The hypha-staining property of Rose Bengal did not prove very important; 3) According to the recent stage of our studies, the ISO 7954-Dichloran combination can be recommended for the mycological evaluation of hays and dried roughages. CFU can be enumerated early, Mucor suppressed in the same extent as with DRBC and colours are easily observable; 4) 2-4-Dinitrosalicylic-acid (DSA) proved unsuitable as inhibitor, for its poor efficiency and for its intense yellow background coulour.
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Lack of polymorphism of the agouti signaling protein (ASIP) gene among four different brown hare (Lepus europaeus Pallas 1778) populations
81-85Views:380The brown hare (Lepus europaeus Pallas 1778) is a common palearctic and a popular game species therefore it has been an obvious subject for population genetic studies since the second part of the 20th century. Among the several mitochondrial DNA studies some have been carried out concerning nuclear genes as well. The agouti signaling protein gene (ASIP) is involved in regulating the synthesis of eumelanin and pheomelanin in melanocytes of mammals. Though many studies focused on it in relation with several mammalian species, minimal information is available on this topic concerning the brown hare.
Here we present a short communication concerning the agouti signaling protein (ASIP) gene in four different country’s L. europaeus populations, namely Lithuania, Hungary, Serbia and Georgia. N=45 tissue samples have been investigated from overall 17 sampling sites of the different countries. There has not been found any polymorphism among the sequences. In an alignment with other Leporid species’ partial ASIP sequences downloaded from ENA we have found that based on a 178 base pairs long DNA sequence the haplotype of our samples contains three other Lepus species as well. This is concordant with the findings of a previous study focusing predominantly on the European rabbit (Orycto lagus cuniculus Linnaeus 1758) and the several mutations of its ASIP gene.
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The Effect of Year and Irrigation on the Yield Quantity and Quality of the Potato
12-16Views:458In Hungary, the growing area of potato area reduced dramatically in the last few decades, additionally we are lagging behind the Western European countries as regards yields and the competitiveness of production is further decreased by the great alternation in yields from year to year, the unpredictable market conditions, bad consumption habits and many times unfortunately the lack of quality products.
The ecological and climatic conditions of Hungary are not everywhere suitable for potato, in the area of Debrecen the amount of rainfall was lower, and the monthly average temperature was higher than the requirement of potato in its growing season in 2002 and 2003.
The experiment was carried out at the experimental site of the University of Debrecen, Farm and Regional Research Institute, at Látókép. In our experiment we examined the yield and some quality parameters of 8 and 9 medium-early varieties in large parcels in 2002 and 2003 respectively. Out of the examined varieties 3 are of Dutch, and 6 are of Hungarian breeding.
The experiment was set up on 49.5 m2 parcels on calcareous chernozem soil after winter wheat as a forecrop in both years. The 9 varieties were examined in 4 repetitions in randomized blocks, out of which two repetitions were irrigated, and two were non-irrigated.
We examined the yields of the varieties, the distribution of tubers according to size and their percentages, and the changes in specific parameters of quality and inner content due to irrigation. We studied the dry matter content, the starch content, the under-water mass, the amount of reducing sugars, the colour index of frying and the element contents of tubers.
Summing up, it can be stated that among the agrotechnical year effect, variety and irrigation factors have considerable impact on potato yield quality and quantity. On the basis of our results, it can be stated that in potato production variety should be chosen in accordance with the aim of production and technology should be adapted to that specific variety.