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Perspectives of the livestock sector in the Philippines: A review
175-188Views:4220The Philippine livestock industry is a vital component in the country's agricultural sector. It contributes around 18.23 percent of the gross output value in agriculture and provides livelihood to many people living in rural areas, signifying its importance with regard to the country’s economy. The constantly increasing demand for animal protein associated with the increase of the world’s population led to the intensification of livestock production, realized through the various initiatives implemented by the government, such as the import of high producing purebreds of various species. This strategy greatly influenced the country's animal genetic resource's diversity and increased food animals' population; however, it impacted the native breeds of food animals. Understanding the characteristics, performance and value of native and exotic breeds is essential to prioritize their livestock industry existence. Thus, this paper aims to characterize various native and exotic breeds of livestock in the Philippines and assess the current conservation of native animals.
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Evaluation of deep-frozen ram semen from different sheep breeds with live/dead acrosome staining
26-28Views:215It was found that the Kovács – Foote staining is properly adopted to examine deep-frozen ram’s semen. Data are appropriate for comparison. Examination of one ram’s semen per breed is not enough for drawing any conclusions; therefore, I will continue this research.
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Genetic diversity study of Hungarian honey bee (Apis mellifera L.) colonies – Previous announcement
61-64Views:616In Hungary, the Apismelliferacarnica is the native breed which is the only recognised and breedable honey bee in the country. It is assumed that there are a number of non-native and hybrid honey bee breeds in Hungary. The microsatellite and mitochondrial DNA surveys applied here will be utilised to easily and accurately identify the various sprads, and open up new ways in the research of honey bees. The isolation of the genomial DNS from 5 to 7 day old larvae samples was successfully carried out. In the future the plan is to carry out the measure with microsatellite markers. As an initial step the optimal annealing temperature was identified. In the mitochondrial DNA survey the COI-COII mytochonrial regional primer due to its low anneling temperature cannot be used with any normal sequencing methods. By using these method the aim of this research is the measurement of genetic variance.
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Coagulase-negative staphylococci in ewe udder surface and raw milk samples
221-225Views:489Coagulase-negative staphylococci (CNS) are among the major causes of subclinical mastitis in dairy ewe flocks. This has a financially significant impact on the ewe dairy sector and consumer health. The determination of the bacterial count, particularly CNS, is critical in terms of the quantity and quality of ovine milk. Thus, the purpose of this study was to quantify the CNS count in the udder surface and raw milk samples of the ewe, in addition to identifying CNS strains (n=8) collected from udder surface and individual raw milk samples by 16S rRNA gene sequencing. A total of 164 samples of udder surface and raw milk originated from four sheep farms were tested. The obtained values were compared between the different farms. Furthermore, values during 2018 and 2019 in the case of Farm I were compared. Significantly higher (p<0.05) average count was observed in udder surface samples taken from Farm I (2.8±1.0 lg CFU/cm2) than that of Farm III samples (2.3±0.6 lg CFU/cm2). In the case of individual raw milk, the higher value was observed in samples derived from Farm III (3.5±0.9 lg CFU/mL), while the obtained value was significantly lower (p<0.05) in samples originated from Farm IV (1.8±0.4 lg CFU/mL). In the bulk tank milk samples, the highest mean value was 5.3±0.4 CFU/mL, and there was no significant (p>0.05) variation between farms. Coagulase-negative staphylococci counts were decreased to a certain extent in both sample types tested during 2019 except for individual raw milk derived from the Tsigai breed. The correlation between the mean CNS counts of udder surface and individual raw milk was very weak (r=0.048). Staphylococcus simulans, Staphylococcus auricularis, and Staphylococcus equorum were identified by molecular sequencing and Staphylococcus simulans were the most frequently identified CNS species. A higher CNS count of bulk tank milk than individual raw milk indicates possible contamination during milking and storage. Therefore, further studies are required to investigate the other sources of bulk tank milk contamination to improve the hygienic quality of milk.