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Management of phytopathogens by application of green nanobiotechnology: Emerging trends and challenges
15-22Views:522Nanotechnology is highly interdisciplinary and important research area in modern science. The use of nanomaterials offer major advantages due to their unique size, shape and significantly improved physical, chemical, biological and antimicrobial properties. Physicochemical and antimicrobial properties of metal nanoparticles have received much attention of researchers. There are different methods i.e. chemical, physical and biological for synthesis of nanoparticles. Chemical and physical methods have some limitations, and therefore, biological methods are needed to develop environment-friendly synthesis of nanoparticles. Moreover, biological method for the production of nanoparticles is simpler than chemical method as biological agents secrete large amount of enzymes, which reduce metals and can be responsible for the synthesis and capping on nanoparticles.
Biological systems for nanoparticle synthesis include plants, fungi, bacteria, yeasts, and actinomycetes. Many plant species including Opuntia ficus-indica, Azardirachta indica, Lawsonia inermis, Triticum aestivum, Hydrilla verticillata, Citrus medica, Catharanthus roseus, Avena sativa, etc., bacteria, such as Bacillus subtilis, Sulfate-Reducing Bacteria, Pseudomonas stutzeri, Lactobacillus sp., Klebsiella aerogenes, Torulopsis sp., and fungi, like Fusarium spp. Aspergillus spp., Verticillium spp., Saccharomyces cerevisae MKY3, Phoma spp. etc. have been exploited for the synthesis of different nanoparticles. Among all biological systems, fungi have been found to be more efficient system for synthesis of metal nanoparticles as they are easy to grow, produce more biomass and secret many enzymes. We proposed the term myconanotechnology (myco = fungi, nanotechnology = the creation and exploitation of materials in the size range of 1–100 nm). Myconanotechnology is the interface between mycology and nanotechnology, and is an exciting new applied interdisciplinary science that may have considerable potential, partly due to the wide range and diversity of fungi.
Nanotechnology is the promising tool to improve agricultural productivity though delivery of genes and drug molecules to target sites at cellular levels, genetic improvement, and nano-array based gene-technologies for gene expressions in plants and also use of nanoparticles-based gene transfer for breeding of varieties resistant to different pathogens and pests. The nanoparticles like copper (Cu), silver (Ag), titanium (Ti) and chitosan have shown their potential as novel antimicrobials for the management of pathogenic microorganisms affecting agricultural crops. Different experiments confirmed that fungal hyphae and conidial germination of pathogenic fungi are significantly inhibited by copper nanoparticles. The nanotechnologies can be used for the disease detection and also for its management. The progress in development of nano-herbicides, nano-fungicides and nano-pesticides will open up new avenues in the field of management of plant pathogens. The use of different nanoparticles in agriculture will increase productivity of crop. It is the necessity of time to use nanotechnology in agriculture with extensive experimental trials. However, there are challenges particularly the toxicity, which is not a big issue as compared to fungicides and pesticides.
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Assessment of Environmental Susceptibility/Vulnerability of Soils
62-74Views:195Soils represent a considerable part of the natural resources of Hungary. Consequently, rational land use and proper soil management – to guarantee normal soil functions – are important elements of sustainable (agricultural) development, having special importance both in the national economy and in environment protection.
The main soil functions in the biosphere are as follows: conditionally renewable natural resource; reactor, transformer and integrator of the combined influences of other natural resources (solar radiation, atmosphere, surface and subsurface waters, biological resources), place of „sphere-interactions”; medium for biomass production, primary food-source of the biosphere; storage of heat, water and plant nutrients; natural filter and detoxication system, which may prevent the deeper geological formations and the subsurface waters from various pollutants; high capacity buffer medium, which may prevent or moderate the unfavourable consequences of various environmental stresses; significant gene-reservoir, an important element of biodiversity.
Society utilizes these functions in different ways (rate, method, efficiency) throughout history, depending on the given natural conditions and socio-economic circumstances. In many cases the character of the particular functions was not properly taken into consideration during the utilization of soil resources, and the misguided management resulted in their over-exploitation, decreasing efficiency of one or more soil functions, and – over a certain limit – serious environmental deterioration.
Soil resources are threatened by the following environmental stresses:
– soil degradation processes;
– extreme moisture regime;
– nutrient stresses (deficiency or toxicity);
– environmental pollution.
Environmental stresses caused by natural factors or human activities represent an increasing ecological threat to the biosphere, as well as a socio-economic risk for sustainable development, including rational land use and soil management.
The stresses are caused by the integrated impacts of various soil properties, which are the results of soil processes (mass and energy regimes, abiotic and biotic transport and transformation and their interactions) under the combined influences of soil forming factors. Consequently, the control of soil processes is a great challenge and the main task of soil science and soil management in sustainable development.
The efficient control of these processes necessitates the following consecutive steps:
• registration of facts and consequences (information on land and soil characteristics, land use, cropping pattern, applied agrotechnics, yields, with their spatial and temporal variability);
• evaluation of potential reasons (definition and quantification of soil processes, analysis of influencing factors and their mechanisms);
• assessment of the theoretical, real, rational and economic possibilities for the control of soil processes (including their risk-assessment and impact analysis);
• elaboration of efficient technologies for the „best” control alternatives (best management practice).
Scientifically based planning and implementation of sustainable land use and rational soil management to ensure desirable soil functions, without any undesirable environmental side-effects, require adequate soil information. In the last years such data were organized into a computer-based GIS soil database in Hungary, giving opportunities for the quantification, analysis, modelling and forecasting of the studied environmental stresses and for the efficient and scientifically based prevention, elimination or reduction of environmental stresses and their unfavourable ecological and economical consequences.
Special attention was paid to the assessment of various soil degradation processes, as: (1) soil erosion by water or wind; (2) soil acidification; (3) salinization and/or alkalization; (4) physical degradation (structure destruction, compaction); (5) extreme moisture regime: drought sensitivity and waterlogging hazard; (6) biological degradation; (7) unfavourable changes in the plant nutrient regime; (8) decrease of natural buffering capacity, (9) soil (and water) pollution.
The actions against undesirable environmental stresses and their unfavourable consequences are important elements of sustainable, efficient, economically viable, socially acceptable and environmentally sound crop production and agricultural development. These are joint tasks of the state, decision makers on various levels, the land owners, the land users and – to a certain extent – of each member of the society. -
Production of novel fermented milks
303-305Views:259The objective of this research was to test the influence of various natural substances on acid production, growth, and viability of characteristic microorganisms in yogurt and probiotic fermented dairy foods. Oligofructose, inulin, honey, and the dried biomass of Spirulina (Arthrospira) platensis were found to stimulate the growth rate and acid production activity of the major thermophilic diary cultures tested and, in addition to this, the presence of the aforementioned substrates also improved the survival of starter bacteria in fermented milk products during storage. The reduced production time of cultured milks resulted in increased production efficiency. The stimulatory and/or protective effect of oligofructose,
inulin, honey, and Spirulina on Bifidobacterium spp. is probably the most important finding of this study because bifidobacteria do not grow well in milk and they have low survival rates in conventional fermented milks. Some of the bioactive substances tested were also capable of exerting an antifungal effect on spoilage yeasts and molds, and improving the nutritional and sensory properties of the final product, thus providing a new opportunity for manufacture of functional fermented dairy foods. -
Allelopathic effect of invasive plants (Eriochloa villosa, Asclepias syriaca, Fallopia x bohemica, Solidago gigantea) on seed germination
179-182Views:510The aim of this study was to determine the allelopathic potential of invasive species woolly cupgrass (Eriochloa villosa), common milkweed (Asclepias syriaca), bohemian knotweed (Fallopia x bohemica), and giant goldenrod (Solidago gigantea Ait.) on germination crop (Lepidium sativum L.). Experiments were conducted under laboratory conditions to determine effect of water extracts in petri dish bioassay. Water extracts from fresh biomass (leaves and stem) of invasive weeds in concentrations of 4 and 8 g/100 ml were investigated. All invasive plants showed allelopathic effect on germination. In giant goldenrod stem water extract experiment, allelopathic effect was less pronounced.
The cress germination was greatly suppressed with the woolly cupgrass, common milkweed and the giant goldenrod. The experiment showed that the seed germination depended on the concentrations and the plant material used (leaves and stem).
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Renewable energy on animal farms: support system and practical application
13-17Views:224Renewable energy applications were examined in animal farms. The investment support system for animal farms – which has renewable elements – has been summarized through analyzing the legislation and future prospects. The most important in this topic is the Animal Farm Modernization Support, which had several application periods during the 2007–2013 CAP budgets. For analyzing practical application of renewable energies interviews and farm visits have done in Hajdú-Bihar County. The questions covered the general attributes of the farms and the details of the (existing or planned) renewable energy application. In terms of economic analysis saving, the investment return time and the employment effect were examined. The results show wide variety of applied renewable energy application. The investment supports plays great role in renewable energy projects by lowering the return time; and through additional points in application assessing, which is an incentive for farmers to include renewable energy element in their modernization projects.
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Effect of different sources and doses of sulphur on yield, nutrient content and uptake by spring wheat
109-115Views:337The objective of this study was to investigate the effect of two sulphur forms (sulphate and tiosulphate) in combination with three different N:S ratios on the yield of spring wheat and total N- and S-content and uptake by the aboveground biomass on chernozem and sandy soil. In the greenhouse experiment, the effects of two sulphur forms were compared: sulphate (SO42-) and thiosulphate (S2O32-). The sulphate was applied as potassium-sulphate (K2SO4) and thiosulphate as ammonium-thiosulphate ((NH4)2S2O3). Increasing doses of both sulphur forms (24, 60, 120 kg S ha-1) were used with the same nitrogen dose (120 kg N ha-1) which caused three different N:S ratios background (1:0.2, 1:0.5, 1:1). Nitrogen was supplied in the form of monoammonium-phosphate (MAP), ammonium-nitrate and ammonium-thiosulphate. Plant samples were taken in three different development stages of spring wheat based on the BBCH scale: at the stage of BBCH 30–32 (stem elongation), BBCH 65–69 (flowering) and BBCH 89 (ripening). The total nitrogen and total sulphur content of plant at different development stages and also wheat grain were measured by Elementar Vario EL type CNS analyser. The nutrient uptake by plant and grain was calculated from the yield of spring wheat and the N and S content of plant. The grain yield on chernozem soil ranged between 6.31 and 12.13 g/pot. All fertilised treatments significantly increased the grain yield compared to the control. The highest yield was obtained in the case of the application of 120 kg N ha-1 and 60 kg S ha-1in sulphate form. The grain yield on sandy soil varied from 2.53 to 6.62 g/pot. The fertilised treatments significantly enhanced the yield compared to the control. The highest yield was observed in the case of the application of 120 kg N ha-1 and 60 kg S ha-1 in thiosulphate form. On chernozem soil the increasing doses of sulphur (24, 60, 120 kg S ha-1) with the same N dose (120 kg N ha-1) increased the N-content of spring wheat at all development stages and in the grain. The treatments with different sulphur sources did not cause further changes in the N-content. On sandy soil in the most cases the N-content did not change significantly as a result of increasing sulphur doses. The treatments with sulphate form basically resulted higher nitrogen-content than treatments with thiosulphate form. The treatments with increasing sulphur doses resulted higher S-content on both of chernozem and sandy soil in the case of all development stage. Comparing the effect of the applied sulphur sources on the S-content it can be stated that at the stage of BBCH 30–31 and 65–69 the treatments with sulphate form resulted higher sulphur-content. At the stage of BBCH 89 there was no significant differences in S-content of grain as a result of different sulphur-sources.
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Magnesium uptake dynamism of maize (Zea mays L.) on prairie soil
83-89Views:145Different influence factors on the magnesium (Mg) uptake in case of three maize hybrids with different long vegetation period have been investigated at the Experimental Station of the University of Debrecen, Centre of Agricultural Sciences and Engineering, at Debrecen-Látókép. The soil of the experiment is a calcareous chernozem, based on loess, with high fertility, that is characteristic for soils of the region Hajdúság.
Upon irrigation is the experiment divided to main plots, by different hybrids into sub-plots, while treatments of five nutrientsupply levels with fixed N:P2O5:K2O rate (beside control)mean sub-subplots. Soil samples were taken from the upper, cultivated soil layer 3 times during the year 2008. Their pH has been measures in a 0.01 M CaCl2-solution and their Mg-content from the same solution and from ammonium-lactate acetic acid (AL) extract. Plant samples were taken seven times in the vegetation period, of which we measured the Mg-content. Beside this, the during the
vegetation period by maize biomass extracted Mg-amount has been calculated using fresh and dry matter weights. The effects of irrigation, hybrids and nutrient-supply levels on the soil pH and on the AL- and CaCl2-extractable Mg-amount have been studied, as well. After that I tried to find a correlation between soil pH and the Mg-content of soil determined in different extractants, beside this between the by the two solutions extracted Mg-amount. -
Evaluation of Soil Degradation Based on High Resolution Remote Sensing Data
145-148Views:268Soil salinity is the main problem of soil degradation in the Grate Plain with cultivated area of 20% affected. Its influence is accelerated on the water managed and irrigated lands. Remote sensing can significantly contribute to detecting temporal changes of salt-related surface features. We have chosen a farm where intensive crop cultivation takes place as a test site as soil degradation can be intensive as a result of land use and irrigation. In order to evaluate soil salt content and biomass analysis, we gathered detailed data from an 100x250 m area. We analyzed the salinity property of the samples. In our research we used a TETRACAM ADC multispectral camera to take high resolution images (0,2-0,5 m) of low altitude (300-500 m). A Normalized Vegetation Index was computed from near infrared (750-950 nm) and red (620-750 nm) bands. This data was compared with the samples of investigated area. Analyzing the images, we evaluated image reliability, and the connection between the bands and the soil properties (pH, salt content). A strong correlation observed between NDVI and soil salinity (EC) makes the multispectral images suitable for construction of salinity map. A further strong correlation was determined between NDVI and yield.
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Estimation of the vine-shoot yield in the Gyöngyös district
21-26Views:208Every year, a significant amount of vineyard prunings is generated in Hungary. Instead of utilization them, it is burned directly on the field in spite of the limitations of legalization highly pollute the environment in recent decades. The vineyard pruning itself or with other by-products from pomology and forestry can be significant amount of fuel source. However, before the planning to utilization the essential task is to estimate the potential. This study examine that how many vineyard is generated in the micro-region of Gyöngyös in every year on appointed vineyard.
In order to vine-branch potential calculate more accurate data was necessary so the products had to be determined for all plant. Thus, the biomass resource and the utilization become plannable. As the result of the experiment we can estimate that 1,5 t/ha vineyard pruning are generated. It means more than 9 thousand tons pruning in the micro-region of Gyöngyös. This amount can be convert into fuel source more than 150 thousand GJ heating value.
The results evince that the vineyard pruning is important fuel source in every year in the micro-region of Gyöngyös. Further usage is suggested for other regions in other size.
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The effects of bioactive feed additives for the production parameters of common carp (Cyprinus carpio) fingerlings in intensive recirculating system
35-41Views:388For the appropriate quantity and quality of fish meat the pond farmers need to use up to date nutrition and feeding technologies. Recently the intensification of the carp production is stepping up with the availability the proper quality of the artificial feeds. The using of different feed additives make a better feed conservation ratio, faster growing rates and more uniform stocks.
In our experiment what is made with common carp (duration of the experiment was 8 weeks long) we compared two different kinds of feed additives in different doses. One of them contains only natural mineral elements and the other additive is a natural origin probiotics. Both additives were used in the quantity of 0.5, 1 and 2% of the total feed. By the result of the 8 weeks experiment it could be said, that the using of the humic acid based mineral elements feed additive. Makes a better FCR comparing with the control stock, but was not as good as the using of the probiotics. At the humic acid treatments the more doses, the better FCR value, but at the probiotics it wasn’t true. The 0.5% probiotic treatment gave the best result. It is concluded that the using of the probiotic feed additives always gave a better performance of the fishes (average weight, biomass, FCR) than the humic acid treatments. The using of the feed additives hasn’t got an influence for the survival rate of the carps.