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• Investigation of the cellular internalization of hydroxypropyl-beta-cyclodextrin and its effects on lysosomes

  57-64

  Ágnes Rusznyák

  Katalin Réti-Nagy

  Judit Váradi

  Ildikó Bácskay

  Miklós Vecsernyés

  Ferenc Fenyvesi

  Views:

  57

  Cyclodextrins are widely used excipients to increase water solubility and bioavailability of lipophilic drugs. Our research group previously showed that cyclodextrins are able to enter Caco-2 intestinal epithelial cells by endocytosis, however, the cellular internalization and intracellular effects in the case of different cells have not been investigated yet. In addition, 2-hydroxypropyl-beta-cyclodextrin (HPBCD) is a potential molecule for the treatment of Niemann Pick syndrome type C due to its cholesterol complexing properties. The aim of our work is to investigate and compare the cellular internalization of HPBCD and its effect on lysosomes on Caco-2 intestinal epithelial, HeLa cervical epithelial and hCMEC/D3 brain endothelial cells.

• Role of cyclodextrins in therapeutic strategies of COVID-19

  Katalin Réti-Nagy

  Views:

  116

  Since the emergence of the SARS-CoV-2 virus in late 2019, several therapeutic possibilities have appeared to treat patients who are often in severe condition due to the pathogen. As more information  becomes available about the virus, the therapeutic strategies can be more specific. Cyclodextrins are well known molecules in the pharmaceutical industry. Due to their special structure they can be widely used in drug development. One of our main research topic is the application of cyclodextrins, so considering the current pandemic situation, it was relevant for us to summarize the role of cyclodextrin molecules in the therapy of COVID-19 disease. In the course of our literary research, we have found many interesting theories and promising developments.

• Formulation of Novel Liquid Crystal (LC) Formulations with Skin-Permeation-Enhancing Abilities of Plantago lanceolata (PL) Extract and Their Assessment on HaCaT Cells

  Zoltán Ujhelyi

  Views:

  113

  Exposure to reactive oxygen species can easily result in serious diseases, such as hyperproliferative skin disorders or skin cancer. Herbal extracts are widely used as antioxidant sources in different compositions. The importance of antioxidant therapy in inflammatory conditions has increased. Innovative formulations can be used to improve the effects of these phytopharmacons. The bioactive compounds of Plantago lanceolata (PL) possess different effects, such as anti-inflammatory, antioxidant, and bactericidal pharmacological effects. The objective of this study was to formulate novel liquid crystal (LC) compositions to protect Plantago lanceolata extract from hydrolysis and to improve its effect. Since safety is an important aspect of pharmaceutical formulations, the biological properties of applied excipients and blends were evaluated using assorted in vitro methods on HaCaT cells. According to the antecedent toxicity screening evaluation, three surfactants were selected (Gelucire 44/14, Labrasol, and Lauroglycol 90) for the formulation. The dissolution rate of PL from the PL-LC systems was evaluated using a Franz diffusion chamber apparatus. The antioxidant properties of the PL-LC systems were evaluated with 2,2-diphenyl-1-picrylhydrazyl (DPPH) and malondialdehyde (MDA) assessments. Our results suggest that these compositions use a nontraditional, rapid-permeation pathway for the delivery of drugs, as the applied penetration enhancers reversibly alter the barrier properties of the outer stratum corneum. These excipients can be safe and highly tolerable thus, they could improve the patient’s experience and promote adherence.